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The map label for this gene is ybiS [H]

Identifier: 218548340

GI number: 218548340

Start: 989324

End: 990244

Strand: Reverse

Name: ybiS [H]

Synonym: EFER_0962

Alternate gene names: 218548340

Gene position: 990244-989324 (Counterclockwise)

Preceding gene: 218548342

Following gene: 218548337

Centisome position: 21.58

GC content: 51.57

Gene sequence:

>921_bases
ATGAACATGAAATTGAAAACGTTATTCGCAGCGGCCTTCGCTGTTGTCGGCTTTTGCAGTACCGCTTCTGCGGTAACTTA
TCCCCTGCCAACCGACGGGAGTCGCCTGGTTGGTCAGAATCAGGTCGTGACCATTCCGGAAGGTAACTCTCAGCCGCTGG
AGTACTTTGCGACCGAGTACCAGATGGGCTTATCTAACATGATGGAAGCTAACCCAGGTGTGGATATCTTCCTGCCAAAA
GGCGGTACAGTACTGAATATTCCTCAGCAACTGATCCTGCCGGATACTGTTCATGAAGGTATCGTCATCAACAGCGCAGA
AATGCGTCTGTACTACTATCCGAAAGGCACCAACACCGTAATTGTGCTACCAATTGGTATTGGTCAGTTAGGTAAAGACA
CCCCAATCAACTGGACGACGAAGGTTGAACGTAAGAAAGCAGGCCCGACCTGGACGCCAACCGCCAAAATGCACGCAGAG
TACCGCGCTGCGGGCGAACCGCTTCCGGCGGTCGTTCCGGCTGGCCCGGATAACCCGATGGGGCTTTATGCATTGTATAT
CGGTCGTCTGTATGCGATTCACGGCACCAACGCTAACTTCGGCATTGGCTTGCGTGTAAGTCACGGTTGTGTGCGTCTGC
GTAATGAAGATATCAAGTTCCTGTTCGAGCAAGTTCCCGTTGGAACTCGCGTACAGTTTATTGATGAGCCAGTGAAAGCA
ACCACCGAACCAGATGGCAGCCGTTACATTGAAGTCCATAACCCGCTGTCTACCACGGAAGCGCAATTTGAAGGTCAGGA
AATTGTGCCGATCAAGCTGACGAAGAGTGTGCAGAGTGTCACCAGCCAGCCAGACGTCGATCAGGTTGTTCTGGATGAAG
CGATTAAAAACCGTTCCGGGATGCCCGTTCGTCTGAATTAA

Upstream 100 bases:

>100_bases
ACATCTGATTTCACTGTGAGCAGGAATGAACTTATAATGCGCTTCCAATACTCTAATATTCTCAACCCAATGGCCTGCCA
GGCACTAAATCTCGCATAAC

Downstream 100 bases:

>100_bases
TCTTCACTGACTGAATGTAAAAGGCGGACTGATAATCCGCCTTTTTTATTTGCCAGCCCTTACCCAGCCTTGAGTAGAAT
CAACCCATATCCTACCAGAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVVTIPEGNSQPLEYFATEYQMGLSNMMEANPGVDIFLPK
GGTVLNIPQQLILPDTVHEGIVINSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDIKFLFEQVPVGTRVQFIDEPVKA
TTEPDGSRYIEVHNPLSTTEAQFEGQEIVPIKLTKSVQSVTSQPDVDQVVLDEAIKNRSGMPVRLN

Sequences:

>Translated_306_residues
MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVVTIPEGNSQPLEYFATEYQMGLSNMMEANPGVDIFLPK
GGTVLNIPQQLILPDTVHEGIVINSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDIKFLFEQVPVGTRVQFIDEPVKA
TTEPDGSRYIEVHNPLSTTEAQFEGQEIVPIKLTKSVQSVTSQPDVDQVVLDEAIKNRSGMPVRLN
>Mature_306_residues
MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVVTIPEGNSQPLEYFATEYQMGLSNMMEANPGVDIFLPK
GGTVLNIPQQLILPDTVHEGIVINSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDIKFLFEQVPVGTRVQFIDEPVKA
TTEPDGSRYIEVHNPLSTTEAQFEGQEIVPIKLTKSVQSVTSQPDVDQVVLDEAIKNRSGMPVRLN

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=306, Percent_Identity=97.3856209150327, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI1788299, Length=296, Percent_Identity=63.8513513513513, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787356, Length=311, Percent_Identity=48.5530546623794, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1787968, Length=305, Percent_Identity=45.2459016393443, Blast_Score=238, Evalue=3e-64,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 33383; Mature: 33383

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVVTIPEGNSQPLEYFATEY
CCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCEEEECCCCCCCHHHHHHHHH
QMGLSNMMEANPGVDIFLPKGGTVLNIPQQLILPDTVHEGIVINSAEMRLYYYPKGTNTV
HHHHHHHHHCCCCEEEEECCCCEEEECCHHHCCCCCCCCCEEEECEEEEEEEEECCCCEE
IVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAEYRAAGEPLPAVVPAGPDNPM
EEEEECCCCCCCCCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCC
GLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDIKFLFEQVPVGTRVQFIDEPVKA
HHHHEEEEEEEEEECCCCCEEEEEEEECCEEEECCCHHHHHHHHCCCCCEEEEECCCCCC
TTEPDGSRYIEVHNPLSTTEAQFEGQEIVPIKLTKSVQSVTSQPDVDQVVLDEAIKNRSG
CCCCCCCEEEEECCCCCCCHHHCCCCEEEEEEECHHHHHHHCCCCHHHHHHHHHHHCCCC
MPVRLN
CCEEEC
>Mature Secondary Structure
MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVVTIPEGNSQPLEYFATEY
CCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCEEEECCCCCCCHHHHHHHHH
QMGLSNMMEANPGVDIFLPKGGTVLNIPQQLILPDTVHEGIVINSAEMRLYYYPKGTNTV
HHHHHHHHHCCCCEEEEECCCCEEEECCHHHCCCCCCCCCEEEECEEEEEEEEECCCCEE
IVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAEYRAAGEPLPAVVPAGPDNPM
EEEEECCCCCCCCCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCC
GLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDIKFLFEQVPVGTRVQFIDEPVKA
HHHHEEEEEEEEEECCCCCEEEEEEEECCEEEECCCHHHHHHHHCCCCCEEEEECCCCCC
TTEPDGSRYIEVHNPLSTTEAQFEGQEIVPIKLTKSVQSVTSQPDVDQVVLDEAIKNRSG
CCCCCCCEEEEECCCCCCCHHHCCCCEEEEEEECHHHHHHHCCCCHHHHHHHHHHHCCCC
MPVRLN
CCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]