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The map label for this gene is ackA [H]

Identifier: 218548254

GI number: 218548254

Start: 886936

End: 888138

Strand: Reverse

Name: ackA [H]

Synonym: EFER_0872

Alternate gene names: 218548254

Gene position: 888138-886936 (Counterclockwise)

Preceding gene: 218548255

Following gene: 218548253

Centisome position: 19.35

GC content: 49.88

Gene sequence:

>1203_bases
ATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGA
AGAGTACCTTTCTGGTTTAGCCGAATGTTTCCATCTTCCTGAAGCCCGTATCAAATGGAAAATGGACGGCAATAAACAAG
AAGCGGCTTTAGGTGCAGGCGCCGCTCACAGTGAAGCGCTTAACTTTATCGTTAACACTATTCTGGCACAAAAACCAGAA
CTGTCTGCGCAGCTGACTGCTATCGGTCACCGTATCGTTCACGGTGGTGAAAAATACACCAGCTCCGTCGTTATCGATGA
CTCTGTTATCCAGGGTATCAAAGATGCAGCCTCTTTTGCACCGCTGCATAACCCTGCACACCTGATCGGTATCGAAGAAG
CTCTGAAATCTTTCCCGCAACTGAAAGACAAGAACGTAGCGGTATTCGATACTGCGTTCCATCAGACAATGCCGGAAGAA
TCTTACCTCTACGCCCTGCCGTACAATCTGTATAAAGAGCATGGTATTCGTCGCTACGGCGCACACGGCACCAGCCACTT
CTATGTTACTCAGGAAGCGGCAAAAATGCTGAACAAACCGGTAGAAGAACTGAACATCATTACCTGCCACCTGGGTAACG
GCGGTTCTGTTTCTGCTATCCGTAACGGCAAATGTGTTGACACCTCTATGGGTCTGACTCCGCTGGAAGGCCTGGTAATG
GGTACTCGCTCTGGCGATATCGATCCGGCGATCATCTTCCATCTGCACGACACTCTGGGCATGAGCGTAGACGCCATCAA
CAAAATGCTGACCAAAGAGTCTGGTCTGCTGGGTCTGACCGAAGTGACCAGCGACTGCCGTTATGTTGAAGATAACTACG
CGACTAAAGAAGACGCGAAACGCGCAATGGACGTATACTGCCACCGCCTGGCGAAATACATCGGTGCTTACACAGCACTG
ATGGATGGTCGCCTGGATGCTGTTGTATTCACTGGTGGTATCGGTGAAAACGCAGCGATGGTTCGCGAACTGTCTCTGGG
TAAACTGGGTGTGCTGGGCTTTGAAGTAGATCACGAGCGTAATCTGGCTGCACGTTTTGGCAAATCTGGTTTCATTAACA
AAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGTCTGACTGCC
TGA

Upstream 100 bases:

>100_bases
TCTGAGTGCATGATGTTAATCATAAATGTCATTGTTATCCTGCGCTACGCTGTATGACTCCTGACGTTTTTTTAGCCACG
TATCAATTATAGGTACTTCC

Downstream 100 bases:

>100_bases
TTTCACACCGCCAGCCCTGCTGGCGGTGCTGTTTTGTAACCCGCCCACCTTCGGCGGTAACGAAAGAGGATAAATCGTGT
CCCGTATTATTATGCTGATC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPE
LSAQLTAIGHRIVHGGEKYTSSVVIDDSVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDTLGMSVDAINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTAL
MDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA

Sequences:

>Translated_400_residues
MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPE
LSAQLTAIGHRIVHGGEKYTSSVVIDDSVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDTLGMSVDAINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTAL
MDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
>Mature_399_residues
SSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPEL
SAQLTAIGHRIVHGGEKYTSSVVIDDSVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEES
YLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVMG
TRSGDIDPAIIFHLHDTLGMSVDAINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALM
DGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=99.5, Blast_Score=822, Evalue=0.0,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=44.1919191919192, Blast_Score=317, Evalue=8e-88,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43295; Mature: 43164

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAG
CCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCC
AAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDDSVIQGIKDAASFA
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHCCEEEECHHHHHHHHHHHHHC
PLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYNLYKEHGIRRYG
CCCCCHHEEEHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEEECHHHHHHCCCEECC
AHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDTLGMSVDAINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHER
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCC
NLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
CHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCC
>Mature Secondary Structure 
SSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAG
CCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCC
AAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDDSVIQGIKDAASFA
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHCCEEEECHHHHHHHHHHHHHC
PLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYNLYKEHGIRRYG
CCCCCHHEEEHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEEECHHHHHHCCCEECC
AHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDTLGMSVDAINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHER
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCC
NLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
CHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]