Click here to switch to the map view.

The map label for this gene is dapE [H]

Identifier: 218548095

GI number: 218548095

Start: 721501

End: 722628

Strand: Reverse

Name: dapE [H]

Synonym: EFER_0706

Alternate gene names: 218548095

Gene position: 722628-721501 (Counterclockwise)

Preceding gene: 218548096

Following gene: 218548094

Centisome position: 15.75

GC content: 52.93

Gene sequence:

>1128_bases
ATGTCGTGCCCGGTTATTGAGCTGACACAACAGCTTATTCGCCGCCCTTCCCTGAGTCCTGATGACGCAGGATGTCAGGC
TTTGTTGATTAAACGTTTGCAGGCGATCGGTTTTACCGTTGAACGCATGGACTTTGCCGATACGCAGAATTTTTGGGCAT
GGCGTGGGCAAGGTGAAACGTTAGCCTTTGCCGGGCATACCGACGTGGTACCGCCTGGCGACGCCGACCGTTGGATTAAT
CCGCCGTTTGAACCAACCATTCGTGACGGCATGTTGTTCGGGCGTGGTGCGGCAGATATGAAAGGCTCACTGGCGGCGAT
GGTGGTAGCAGCAGAACGTTTTGTCGCACAACATCCCAACCATACAGGTCGACTGGCATTTCTGATCACCTCTGATGAAG
AAGCCAGTGCCCATAACGGTACGGTAAAAGTCGTCGACGCGTTAATGGCACGTAATGAGCGTCTCGATTACTGCCTGGTC
GGCGAACCGTCGAGCATCGAAGTGGTTGGTGATGTGGTGAAAAATGGTCGTCGCGGATCGTTAACCTGCAACCTCACCAT
TCATGGCGTTCAGGGGCATGTGGCCTACCCACATCTGGCTGACAATCCGGTACATCGCGCAGCACCTTTCCTTAATGAAT
TAGTGGCTATTGAGTGGGATCAGGGTAATGAATTCTTCCCGGCGACCAGTATGCAGATTGCCAATATTCAGGCGGGAACG
GGCAGTAACAACGTTATTCCGGGTGAACTGTTTGTGCAGTTTAACTTCCGCTTTAGCACCGAACTGACGGATGAGATGAT
CAAAGCGCAGGTGCTTGCCCTGCTTGAAAAACATCAACTGCGTTATACGGTGGATTGGTGGCTTTCCGGGCAGCCATTTT
TGACCGCGCGCGGTAAACTGGTGGATGCGGTCGTTAACGCGGTTGAGCACTATAATGAAATTAAACCGCAGCTACTGACC
ACAGGCGGAACGTCCGACGGGCGCTTTATTGCCCGCATGGGGGCGCAGGTGGTGGAACTCGGGCCGGTCAATGCCACTAT
TCATAAAATTAATGAATGTGTGAACGCTGCCGACCTGCAGCTACTTGCCCGTATGTATCAACGTATCATGGAACAGCTCG
TCGCCTGA

Upstream 100 bases:

>100_bases
TGCAATTATCAAACGCCCATTGCTCTGCGCGCCCGGGAAGCCTATTCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAAT
TTTTCCATGAGGTGTAGTCT

Downstream 100 bases:

>100_bases
TGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGG
CGTCCTGCTGAACGTGATTA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSCPVIELTQQLIRRPSLSPDDAGCQALLIKRLQAIGFTVERMDFADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWIN
PPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVDALMARNERLDYCLV
GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGT
GSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLT
TGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA

Sequences:

>Translated_375_residues
MSCPVIELTQQLIRRPSLSPDDAGCQALLIKRLQAIGFTVERMDFADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWIN
PPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVDALMARNERLDYCLV
GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGT
GSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLT
TGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA
>Mature_374_residues
SCPVIELTQQLIRRPSLSPDDAGCQALLIKRLQAIGFTVERMDFADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINP
PFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVDALMARNERLDYCLVG
EPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGTG
SNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTT
GGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=99.4666666666667, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI1790395, Length=259, Percent_Identity=28.1853281853282, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789236, Length=385, Percent_Identity=22.5974025974026, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17551016, Length=282, Percent_Identity=26.9503546099291, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41255; Mature: 41123

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCPVIELTQQLIRRPSLSPDDAGCQALLIKRLQAIGFTVERMDFADTQNFWAWRGQGET
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEECCCCCE
LAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPN
EEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
HTGRLAFLITSDEEASAHNGTVKVVDALMARNERLDYCLVGEPSSIEVVGDVVKNGRRGS
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCE
LTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGT
EEEEEEEEECCCCEECCCCCCCCHHHHHHHHHCEEEEEECCCCEECCCCCEEEEEEECCC
GSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKL
CCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEECHHH
VDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQ
HHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHCHHHHH
LLARMYQRIMEQLVA
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SCPVIELTQQLIRRPSLSPDDAGCQALLIKRLQAIGFTVERMDFADTQNFWAWRGQGET
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCEEECCCCCE
LAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPN
EEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
HTGRLAFLITSDEEASAHNGTVKVVDALMARNERLDYCLVGEPSSIEVVGDVVKNGRRGS
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCE
LTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGT
EEEEEEEEECCCCEECCCCCCCCHHHHHHHHHCEEEEEECCCCEECCCCCEEEEEEECCC
GSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKL
CCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEECHHH
VDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQ
HHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHCHHHHH
LLARMYQRIMEQLVA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA