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The map label for this gene is pssA

Identifier: 218547888

GI number: 218547888

Start: 488839

End: 490194

Strand: Reverse

Name: pssA

Synonym: EFER_0488

Alternate gene names: 218547888

Gene position: 490194-488839 (Counterclockwise)

Preceding gene: 218547889

Following gene: 218547887

Centisome position: 10.68

GC content: 48.23

Gene sequence:

>1356_bases
ATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGT
CGATTTCTTTTACGCTCCCGCCGACTTCCGGGAGACGCTGCTGGAAAAAATAGCCAGCGCGAAGCAGCGCATTTGCATTG
TCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAACGGCAGCGTCCGGAA
CTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGTGCTGCGGCATCTAACACTAACGCTGA
CTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCAGTTTATGGCGTTCCAATCAATACCCGTGAGGCCC
TTGGTGTTCTGCACTTTAAAGGCTTTATCATTGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTTTACCTG
CATCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTCGAATGGGT
TACACAGAATATAATGAATGGTCGCGGCGTTAATCGTCTGGATGATGTTAATCGTCCAAAAAGCCCGGAAATCAAGAATG
ACATTCGTCTGTTCCGCCAGGAGCTGCGTGATGCCGCTTATCATTTCCAGGGTGATGCCGATAACGATCAGCTTTCTGTT
ACGCCGTTGGTAGGCCTGGGGAAATCGAGTCTTTTGAACAAGACCATTTTCCATCTTATGCCTTGTGCCGAGCAGAAACT
AACCATTTGCACACCATATTTCAACCTGCCAGCAATCCTTGTGCGTAATATCATCCAGTTACTGCGCGAAGGGAAAAAGG
TCGAAATTATTGTTGGCGATAAAACTGCGAATGACTTCTACATTCCGGAAGATGAACCTTTCAAGATAATCGGTGCTCTG
CCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGTTAGTGGTTCG
GTTATGGAAAGACGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGATGCTGATCACCGGCAATA
ACCTGAACCCACGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATTCACGATCCGCAACTGGAACTTGCGCCACAG
CGTGAGAAAGAACTGGAACTGATCCGCGAGCATACCACCATCGTTAAGCACTACCGCGATCTGCAAAGCATTGCCGATTA
TCCGGTGAAGGTTCGTAAACTTATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAA

Upstream 100 bases:

>100_bases
AATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAA
CAGAGAAGAAATGCACTGTG

Downstream 100 bases:

>100_bases
TCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACT
TTCTGGTTGCAGCCATATGG

Product: phosphatidylserine synthase

Products: NA

Alternate protein names: Phosphatidylserine synthase

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKIASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPE
LDVRVLVDWHRAQRGRIGAAASNTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLFRQELRDAAYHFQGDADNDQLSV
TPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNLPAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGAL
PYLYEINLRRFLSRLQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHDPQLELAPQ
REKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRIL

Sequences:

>Translated_451_residues
MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKIASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPE
LDVRVLVDWHRAQRGRIGAAASNTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLFRQELRDAAYHFQGDADNDQLSV
TPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNLPAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGAL
PYLYEINLRRFLSRLQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHDPQLELAPQ
REKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRIL
>Mature_451_residues
MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKIASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPE
LDVRVLVDWHRAQRGRIGAAASNTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLFRQELRDAAYHFQGDADNDQLSV
TPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNLPAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGAL
PYLYEINLRRFLSRLQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHDPQLELAPQ
REKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRIL

Specific function: Unknown

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI87082134, Length=451, Percent_Identity=100, Blast_Score=926, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25143331, Length=461, Percent_Identity=26.0303687635575, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319842, Length=343, Percent_Identity=24.7813411078717, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PSS_ECOLI (P23830)

Other databases:

- EMBL:   M58699
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_003171.1
- RefSeq:   NP_417080.4
- ProteinModelPortal:   P23830
- SMR:   P23830
- DIP:   DIP-10593N
- MINT:   MINT-1218158
- STRING:   P23830
- EnsemblBacteria:   EBESCT00000003388
- EnsemblBacteria:   EBESCT00000016003
- GeneID:   947059
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2569
- KEGG:   eco:b2585
- EchoBASE:   EB0774
- EcoGene:   EG10781
- eggNOG:   COG1502
- GeneTree:   EBGT00050000011574
- HOGENOM:   HBG296973
- OMA:   HLKGFVI
- ProtClustDB:   PRK09428
- BioCyc:   EcoCyc:PHOSPHASERSYN-MONOMER
- BioCyc:   MetaCyc:PHOSPHASERSYN-MONOMER
- Genevestigator:   P23830
- GO:   GO:0005737
- InterPro:   IPR016270
- InterPro:   IPR001736
- PIRSF:   PIRSF000850
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: =2.7.8.8

Molecular weight: Translated: 52803; Mature: 52803

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKIASAKQRICIVALYLEQ
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEEEEEEC
DDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAASNTNADWYCRMAQENPGVD
CCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCCCCCCCCCCEEEEECCCCCCCC
VPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYLHQHDKYRYDRYHLIRNRKMS
CEEEECCCCCHHHHEEEEEEEEEEECHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCHH
DIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLFRQELRDAAYHFQGDADNDQLSV
HHHHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
TPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNLPAILVRNIIQLLREGKKVEIIVGD
EEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECC
KTANDFYIPEDEPFKIIGALPYLYEINLRRFLSRLQYYVNTDQLVVRLWKDDDNTYHLKG
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEE
MWVDDKWMLITGNNLNPRAWRLDLENAILIHDPQLELAPQREKELELIREHTTIVKHYRD
EEECCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LQSIADYPVKVRKLIRRLRRIRIDRLISRIL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKIASAKQRICIVALYLEQ
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEEEEEEC
DDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAASNTNADWYCRMAQENPGVD
CCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCCCCCCCCCCEEEEECCCCCCCC
VPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYLHQHDKYRYDRYHLIRNRKMS
CEEEECCCCCHHHHEEEEEEEEEEECHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCHH
DIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLFRQELRDAAYHFQGDADNDQLSV
HHHHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
TPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNLPAILVRNIIQLLREGKKVEIIVGD
EEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECC
KTANDFYIPEDEPFKIIGALPYLYEINLRRFLSRLQYYVNTDQLVVRLWKDDDNTYHLKG
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEE
MWVDDKWMLITGNNLNPRAWRLDLENAILIHDPQLELAPQREKELELIREHTTIVKHYRD
EEECCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LQSIADYPVKVRKLIRRLRRIRIDRLISRIL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2002065; 9205837; 9278503; 1323044