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The map label for this gene is tpiA [H]

Identifier: 218533005

GI number: 218533005

Start: 5493067

End: 5493831

Strand: Reverse

Name: tpiA [H]

Synonym: Mchl_5129

Alternate gene names: 218533005

Gene position: 5493831-5493067 (Counterclockwise)

Preceding gene: 218533008

Following gene: 218533004

Centisome position: 95.08

GC content: 70.46

Gene sequence:

>765_bases
ATGGCAAGCGAGGGACGCCGTCCGCTGGTTGCCGGCAACTGGAAGATGAACGGTCTGCTCTCCTCGGTCCCGACCGTCGA
GGCGATCCGTGACGGGCTCTCGCCGGCGCTCGCGGATAAGATCGACGTGCTGATCTGCCCGCCCGCGACGCTGATCTCCT
CCTGCGTGGCGGCGGTCTACGGCTCCGTGGTCGCCATCGGCGGCCAGAACCTGCACGCCCGGCCGAGCGGCGCCTTCACC
GGTTCGATCTCGGCGGAGATGTTCGCCGATCTCGGCGCCACCTACGTCATCGTCGGCCATTCCGAGCGGCGCGCCTACCA
TTACGAGACCGATGACGGCGTCCACGCCAAGGCGCTCGGCGCTCGCCGCGCGGGCCTGCGCGGCATCATCTGCGTCGGCG
AGACGATGGAGGAGCGCCAGCAGGGCCGGGCGCTCGACATCGTCCGCGCGCAGCTCGCCATCGGCCTGCCGAAGGGTGCG
ACCGGCGCCGACACCGTGATCGCCTACGAGCCGGTCTGGGCGATCGGCTCGGGCCGCACGCCGACGGCGACGGAGATCGC
CGAGGTCCACGCCTCCTTGCGCGAGATGCTCGACAAGCTCGTCGGCGAGGAGGCGCACAAGATCCGCATCCTCTACGGCG
GCTCGGTGAAGCCCTCGAACGCCCGCGAACTGATGGCGGTGGAGAACGTCGACGGCGCGCTGGTCGGCGGCGCCAGCCTC
GTGGCGGAGGACTTTTTGGGGATCTGCCGCGCCTACGAGGGGTGA

Upstream 100 bases:

>100_bases
CCGGACGATCGCAAAGGTGGTTTGCGCGCCTAAGCTTGCTCTGCTAGGTCCGCCCTAGACGTTGTAAGTCCTCGAACGAG
ACACGGAGAACCGGGACGCC

Downstream 100 bases:

>100_bases
GCCGGTACGGGCCGAGCGGGCCGTCAGCCTCCTTCGATCGGGGCTGCGCGGCACCCGGCTGCTTCTATCGGCGGCCGGAA
CGATCCCCCTTCTCCTGACG

Product: triose-phosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MASEGRRPLVAGNWKMNGLLSSVPTVEAIRDGLSPALADKIDVLICPPATLISSCVAAVYGSVVAIGGQNLHARPSGAFT
GSISAEMFADLGATYVIVGHSERRAYHYETDDGVHAKALGARRAGLRGIICVGETMEERQQGRALDIVRAQLAIGLPKGA
TGADTVIAYEPVWAIGSGRTPTATEIAEVHASLREMLDKLVGEEAHKIRILYGGSVKPSNARELMAVENVDGALVGGASL
VAEDFLGICRAYEG

Sequences:

>Translated_254_residues
MASEGRRPLVAGNWKMNGLLSSVPTVEAIRDGLSPALADKIDVLICPPATLISSCVAAVYGSVVAIGGQNLHARPSGAFT
GSISAEMFADLGATYVIVGHSERRAYHYETDDGVHAKALGARRAGLRGIICVGETMEERQQGRALDIVRAQLAIGLPKGA
TGADTVIAYEPVWAIGSGRTPTATEIAEVHASLREMLDKLVGEEAHKIRILYGGSVKPSNARELMAVENVDGALVGGASL
VAEDFLGICRAYEG
>Mature_253_residues
ASEGRRPLVAGNWKMNGLLSSVPTVEAIRDGLSPALADKIDVLICPPATLISSCVAAVYGSVVAIGGQNLHARPSGAFTG
SISAEMFADLGATYVIVGHSERRAYHYETDDGVHAKALGARRAGLRGIICVGETMEERQQGRALDIVRAQLAIGLPKGAT
GADTVIAYEPVWAIGSGRTPTATEIAEVHASLREMLDKLVGEEAHKIRILYGGSVKPSNARELMAVENVDGALVGGASLV
AEDFLGICRAYEG

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=250, Percent_Identity=38.4, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI226529917, Length=250, Percent_Identity=38.4, Blast_Score=154, Evalue=9e-38,
Organism=Escherichia coli, GI1790353, Length=252, Percent_Identity=42.4603174603175, Blast_Score=181, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=41.2955465587045, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6320255, Length=248, Percent_Identity=41.9354838709677, Blast_Score=187, Evalue=1e-48,
Organism=Drosophila melanogaster, GI28572004, Length=250, Percent_Identity=42.8, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28572008, Length=250, Percent_Identity=42.8, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28572006, Length=250, Percent_Identity=42.8, Blast_Score=173, Evalue=1e-43,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 26564; Mature: 26432

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEGRRPLVAGNWKMNGLLSSVPTVEAIRDGLSPALADKIDVLICPPATLISSCVAAVY
CCCCCCCCEEECCCCCCCHHHCCCHHHHHHHCCCHHHHCCCCEEEECCHHHHHHHHHHHH
GSVVAIGGQNLHARPSGAFTGSISAEMFADLGATYVIVGHSERRAYHYETDDGVHAKALG
HCEEEECCCCCCCCCCCCEECCCHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCHHHHH
ARRAGLRGIICVGETMEERQQGRALDIVRAQLAIGLPKGATGADTVIAYEPVWAIGSGRT
HHHCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEECCCCC
PTATEIAEVHASLREMLDKLVGEEAHKIRILYGGSVKPSNARELMAVENVDGALVGGASL
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEEEEECCCCEEECCHHH
VAEDFLGICRAYEG
HHHHHHHHHHHCCC
>Mature Secondary Structure 
ASEGRRPLVAGNWKMNGLLSSVPTVEAIRDGLSPALADKIDVLICPPATLISSCVAAVY
CCCCCCCEEECCCCCCCHHHCCCHHHHHHHCCCHHHHCCCCEEEECCHHHHHHHHHHHH
GSVVAIGGQNLHARPSGAFTGSISAEMFADLGATYVIVGHSERRAYHYETDDGVHAKALG
HCEEEECCCCCCCCCCCCEECCCHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCHHHHH
ARRAGLRGIICVGETMEERQQGRALDIVRAQLAIGLPKGATGADTVIAYEPVWAIGSGRT
HHHCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEECCCCC
PTATEIAEVHASLREMLDKLVGEEAHKIRILYGGSVKPSNARELMAVENVDGALVGGASL
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHEEEEECCCCEEECCHHH
VAEDFLGICRAYEG
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]