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The map label for this gene is pyrG

Identifier: 218533000

GI number: 218533000

Start: 5489177

End: 5490805

Strand: Reverse

Name: pyrG

Synonym: Mchl_5124

Alternate gene names: 218533000

Gene position: 5490805-5489177 (Counterclockwise)

Preceding gene: 218533001

Following gene: 218532999

Centisome position: 95.03

GC content: 67.46

Gene sequence:

>1629_bases
ATGACGCGGTACGTTTTCATCACCGGCGGCGTGGTTTCCTCCCTGGGCAAGGGCCTCGCCTCCGCCGCCCTCGCGGCTTT
GCTCCAAGCGCGCGGATACCGCGTGCGCCTGCGCAAGCTCGACCCCTATCTCAACGTCGATCCGGGCACGATGAGCCCGA
CGCAGCACGGCGAGGTGTTCGTCACCGACGACGGCGCCGAGACCGACCTGGATCTGGGCCATTACGAGCGCTTCACCGGC
CTGCCGGCCTCGCGCGCCGACAATGTGACCACGGGGCGGATCTACCTCGACATCATCACCAAGGAGCGGCGCGGCGATTA
TCTCGGCGCCACGATCCAGGTGATTCCGCACGTCACCAACGCCATCAAGGCGTTCGTGCTCGACGGCAATGACGATTACG
ACTTCGTTCTGGTCGAGATCGGCGGCACGGTCGGCGACATCGAGGGCCTGCCGTTCTTCGAGGCGATCCGCCAGATCGGC
CAGGAGCGCCCGCGCGGCAGCGTCTGCTACCTGCACCTCACGCTGCTGCCCTACATCCCGTCCGCCGGCGAGTTGAAGAC
CAAGCCGACGCAGCACTCCGTGAAGGAGCTGCGCTCCATCGGCATCCAGCCCGACATCCTGCTCTGCCGCTGCGACCGTC
CGATCCCGCAGGACGAGCGGCGCAAGCTCGGCCTGTTCTGCAACGTGCGGGAAAGCGCCGTCATCGAGGCGCGCGACGTC
GACACGATCTACGCTGTGCCGCTCTCCTACCGCGAGGCGGGACTCGACCGGGAGATCCTGGCACATTTCCAGATGGAGCC
CGAGACCGAGCCGAAGCTCGACCGCTGGCAGAACATCCTCGAGCGGGTGCGCAACCCCGAGGGCGAGGTCACCATCGCCA
TCGTCGGCAAGTATACGGGCCTGAAGGATGCCTACAAGTCGCTCACCGAGGCGCTGACCCACGGTGGTATCGCCAACAAC
GTGCGCGTCAACCTTGAATGGATCGAGGCGGAGGTGTTCGAGCGCGAGGACCCGGCGCCCTTCCTCGAAGGGCTGCACGG
CATCCTCGTGCCCGGCGGCTTCGGCCAGCGCGGCGCCGAGGGCAAGATCCGCGCGGCCCGCTACGCCCGCGAGCGCAACA
TCCCCTATTTCGGCATCTGCTTCGGCATGCAGATGGCGGTGATCGAGGCCGCGCGTTCGCTCGCCGGGATCGAAGGCGCC
AACTCTACTGAGTTCGGCGCGACCGCGGAGCCCGTGGTCGGCCTCCTGACCGAGTGGATGCGCGGCAACGAGTTGGAGCG
GCGCGCGGCCGAGAGCGATCTCGGCGGCACCATGCGGCTCGGCGCCTACAAGGCGACGCTCGGTGCGGACACCAAGATCG
CGCAGATGTACGGCGGCACCGAGATCTCGGAGCGCCACCGGCACCGCTACGAGGTCAACATGGCCTATCGCGCCTGCCTG
GAGGCCAAGGGTCTGCGCTTCTCCGGCACCTCGCCGGACGGGCTGCTGCCCGAGACGGTTGAACACGAGGGCCACCCGTG
GTTCATCGGCGTGCAATTCCACCCGGAGTTGAAGTCGCGCCCGTTCGAGCCGCACCCGCTGTTCAAGGGGTTCATTGCCG
CCGCGATCGACCAGAGCCGGCTGGTGTGA

Upstream 100 bases:

>100_bases
ATGAGGGGCATCGCCCCGTGCCGCCTCCCGCGTGGCCGGCGGGGTGCGGCAACCGTTGCGGTTTGGCGAATCGCTTGAGC
CCCTTTATGGACCAAAGCCC

Downstream 100 bases:

>100_bases
CGTAGGGGAAAGCACGGTTGACGACGATCTCGGGCCTTCCGGCCTTTCTCGCCTACTTCGCCGTCTCCCTCGGGCTCACC
GCCCTCTACCTCGTGACCTA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MTRYVFITGGVVSSLGKGLASAALAALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTG
LPASRADNVTTGRIYLDIITKERRGDYLGATIQVIPHVTNAIKAFVLDGNDDYDFVLVEIGGTVGDIEGLPFFEAIRQIG
QERPRGSVCYLHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRCDRPIPQDERRKLGLFCNVRESAVIEARDV
DTIYAVPLSYREAGLDREILAHFQMEPETEPKLDRWQNILERVRNPEGEVTIAIVGKYTGLKDAYKSLTEALTHGGIANN
VRVNLEWIEAEVFEREDPAPFLEGLHGILVPGGFGQRGAEGKIRAARYARERNIPYFGICFGMQMAVIEAARSLAGIEGA
NSTEFGATAEPVVGLLTEWMRGNELERRAAESDLGGTMRLGAYKATLGADTKIAQMYGGTEISERHRHRYEVNMAYRACL
EAKGLRFSGTSPDGLLPETVEHEGHPWFIGVQFHPELKSRPFEPHPLFKGFIAAAIDQSRLV

Sequences:

>Translated_542_residues
MTRYVFITGGVVSSLGKGLASAALAALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTG
LPASRADNVTTGRIYLDIITKERRGDYLGATIQVIPHVTNAIKAFVLDGNDDYDFVLVEIGGTVGDIEGLPFFEAIRQIG
QERPRGSVCYLHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRCDRPIPQDERRKLGLFCNVRESAVIEARDV
DTIYAVPLSYREAGLDREILAHFQMEPETEPKLDRWQNILERVRNPEGEVTIAIVGKYTGLKDAYKSLTEALTHGGIANN
VRVNLEWIEAEVFEREDPAPFLEGLHGILVPGGFGQRGAEGKIRAARYARERNIPYFGICFGMQMAVIEAARSLAGIEGA
NSTEFGATAEPVVGLLTEWMRGNELERRAAESDLGGTMRLGAYKATLGADTKIAQMYGGTEISERHRHRYEVNMAYRACL
EAKGLRFSGTSPDGLLPETVEHEGHPWFIGVQFHPELKSRPFEPHPLFKGFIAAAIDQSRLV
>Mature_541_residues
TRYVFITGGVVSSLGKGLASAALAALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTGL
PASRADNVTTGRIYLDIITKERRGDYLGATIQVIPHVTNAIKAFVLDGNDDYDFVLVEIGGTVGDIEGLPFFEAIRQIGQ
ERPRGSVCYLHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRCDRPIPQDERRKLGLFCNVRESAVIEARDVD
TIYAVPLSYREAGLDREILAHFQMEPETEPKLDRWQNILERVRNPEGEVTIAIVGKYTGLKDAYKSLTEALTHGGIANNV
RVNLEWIEAEVFEREDPAPFLEGLHGILVPGGFGQRGAEGKIRAARYARERNIPYFGICFGMQMAVIEAARSLAGIEGAN
STEFGATAEPVVGLLTEWMRGNELERRAAESDLGGTMRLGAYKATLGADTKIAQMYGGTEISERHRHRYEVNMAYRACLE
AKGLRFSGTSPDGLLPETVEHEGHPWFIGVQFHPELKSRPFEPHPLFKGFIAAAIDQSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=558, Percent_Identity=42.2939068100358, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI28559085, Length=556, Percent_Identity=42.4460431654676, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=556, Percent_Identity=42.4460431654676, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=556, Percent_Identity=42.4460431654676, Blast_Score=457, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=536, Percent_Identity=58.7686567164179, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=35.632183908046, Blast_Score=392, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=40.4217926186292, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319432, Length=567, Percent_Identity=39.3298059964727, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=43.75, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI21357815, Length=506, Percent_Identity=43.4782608695652, Blast_Score=403, Evalue=1e-112,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_METC4 (B7KUU9)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002423816.1
- ProteinModelPortal:   B7KUU9
- GeneID:   7118999
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_5124
- HOGENOM:   HBG597806
- OMA:   TEWLRGN
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60006; Mature: 59875

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 380-380 ACT_SITE 514-514 ACT_SITE 516-516

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYVFITGGVVSSLGKGLASAALAALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGLPASRADNVTTGRIYLDIITKERRGDYLGATIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCEECEEEEEEEECCCCCCCCCHHHHHHHHHHC
AIKAFVLDGNDDYDFVLVEIGGTVGDIEGLPFFEAIRQIGQERPRGSVCYLHLTLLPYIP
CEEEEEECCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPDILLCRCDRPIPQDERRKLGLFCNVRESAVIEARDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHCCCEEEECCCCEEEEECCC
DTIYAVPLSYREAGLDREILAHFQMEPETEPKLDRWQNILERVRNPEGEVTIAIVGKYTG
CEEEEECCCHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
LKDAYKSLTEALTHGGIANNVRVNLEWIEAEVFEREDPAPFLEGLHGILVPGGFGQRGAE
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEHHCCCCCCHHHHCCCEEEECCCCCCCCCC
GKIRAARYARERNIPYFGICFGMQMAVIEAARSLAGIEGANSTEFGATAEPVVGLLTEWM
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
RGNELERRAAESDLGGTMRLGAYKATLGADTKIAQMYGGTEISERHRHRYEVNMAYRACL
CCCHHHHHHHHCCCCCEEEECEEEEECCCCCHHHHHHCCCCHHHHHHHHEEEHHHHHHHH
EAKGLRFSGTSPDGLLPETVEHEGHPWFIGVQFHPELKSRPFEPHPLFKGFIAAAIDQSR
HHCCCEECCCCCCCCCCHHHCCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHC
LV
CC
>Mature Secondary Structure 
TRYVFITGGVVSSLGKGLASAALAALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGLPASRADNVTTGRIYLDIITKERRGDYLGATIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCEECEEEEEEEECCCCCCCCCHHHHHHHHHHC
AIKAFVLDGNDDYDFVLVEIGGTVGDIEGLPFFEAIRQIGQERPRGSVCYLHLTLLPYIP
CEEEEEECCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPDILLCRCDRPIPQDERRKLGLFCNVRESAVIEARDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHCCCEEEECCCCEEEEECCC
DTIYAVPLSYREAGLDREILAHFQMEPETEPKLDRWQNILERVRNPEGEVTIAIVGKYTG
CEEEEECCCHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
LKDAYKSLTEALTHGGIANNVRVNLEWIEAEVFEREDPAPFLEGLHGILVPGGFGQRGAE
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEHHCCCCCCHHHHCCCEEEECCCCCCCCCC
GKIRAARYARERNIPYFGICFGMQMAVIEAARSLAGIEGANSTEFGATAEPVVGLLTEWM
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
RGNELERRAAESDLGGTMRLGAYKATLGADTKIAQMYGGTEISERHRHRYEVNMAYRACL
CCCHHHHHHHHCCCCCEEEECEEEEECCCCCHHHHHHCCCCHHHHHHHHEEEHHHHHHHH
EAKGLRFSGTSPDGLLPETVEHEGHPWFIGVQFHPELKSRPFEPHPLFKGFIAAAIDQSR
HHCCCEECCCCCCCCCCHHHCCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA