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The map label for this gene is ybdK [C]

Identifier: 218532992

GI number: 218532992

Start: 5482683

End: 5483810

Strand: Reverse

Name: ybdK [C]

Synonym: Mchl_5116

Alternate gene names: 218532992

Gene position: 5483810-5482683 (Counterclockwise)

Preceding gene: 218532993

Following gene: 218532991

Centisome position: 94.91

GC content: 70.04

Gene sequence:

>1128_bases
ATGGCACACGCGTACCGGTTCGGGATCGAGGAAGAGCTGTTCCTGGCCAACAGCCGCACCCGGGCGGCGCCCAGCGAGTC
GGTCTCGGCCTTCCACAAGGCGGCGCGGAGCCGGCTGGAGGGCGTCGAGCGCGAGATGCTGGAGAGCCAAGCCGAGATCT
GCTCCAAGCCCTCAACGAGCTTCGACGAGACCCACGAGGCCCTGGCCAAGCTCCGCACCGGCCTGTGCGGCATCGGCCGC
GAGCACGGCCTGAAGGTCTTTGCCGCCGGCACCCACCCGACCGCGATCTGGACCGACCAGCGCGAGACCGCCAAGGTCCG
CTACCGCAAGATGATCGAAACGCTGCAGATGGTGGGCCGGCGCTCCATCGTCAGCGGCCTGCACGTCCATGTCGAGGTGC
CCGAGCCGGACCAGCGCATCGACCTGATCAACCGGCTGATGCCGTTTCTGCCGGTGCTGCTGGCGCTCTCCACCTCCTCG
CCCTTCTACGAACGCCACCGCACCGGGCTCGCCGGCTACCGCCTGCGGGCCTATGCCGAACTGCCGCGCACCGGTCTGCC
GGAACTGTTCGACGGGGCCGACGACTTCGAGCGCTACGTGCGGGTGATGACGCGGGCGGGTGCCGTCGAGGACGCGACTT
ATTTCTGGTGGCACATCCGCCCGTCGATCCGTTACCCGACCCTAGAATTGCGGGTCGCCGACAGTTGCACCCGGCTCAAA
GACACGCTGGCCATTGCCGCGCTCTACCGATGCCTCGTGCGGCTCTGCGTGCGCCAGCCGGAACTGAACGCCCGGCCGAC
GGGCGCCTCGCGCGGCTTCGTCATGGAGAACCTGTGGCGGGCGCAGCGCGACGGCGTTCACGCCGCCCTGATCGATGAGG
CGGCGGAGGAGGCGGTGCCGGTGCGCCGCCTCGTCGAGCGCGTGCTCGACCTCGTGGCGGAGGATGCCGCGGCGTTGGGC
TGCAACGCGCAGATCGCACATGCCCTCACGATCACCGAATGCGGCTCCAGCGCCGACGGGCAGATCCGGGCCTACGAGGC
CTCGCTCGCCGCGGGCGGCAGCGAGCGCAAGGCGCTGTCCGGCGTCATCGACTGGCTCGCCCGCGAGACCGCGAACTGCG
AGGCGTGA

Upstream 100 bases:

>100_bases
GAACAACCGCCTCGATCGTATCGTTCGTTAACGCACGAACCAATTGGGCCCGTGCTTGCTTCCATCCGGACCGGGTCGGA
CCCGGAAGGTGGGACGAGCA

Downstream 100 bases:

>100_bases
CGGCGCGCCTCCGGCTCCGCCAGGAACATCGAACGCCCGCTCCGGTTGCTCCGACAGGTCCGATTCTGTCCTAGGAGACT
TCCATGAGAACGACACTCAC

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MAHAYRFGIEEELFLANSRTRAAPSESVSAFHKAARSRLEGVEREMLESQAEICSKPSTSFDETHEALAKLRTGLCGIGR
EHGLKVFAAGTHPTAIWTDQRETAKVRYRKMIETLQMVGRRSIVSGLHVHVEVPEPDQRIDLINRLMPFLPVLLALSTSS
PFYERHRTGLAGYRLRAYAELPRTGLPELFDGADDFERYVRVMTRAGAVEDATYFWWHIRPSIRYPTLELRVADSCTRLK
DTLAIAALYRCLVRLCVRQPELNARPTGASRGFVMENLWRAQRDGVHAALIDEAAEEAVPVRRLVERVLDLVAEDAAALG
CNAQIAHALTITECGSSADGQIRAYEASLAAGGSERKALSGVIDWLARETANCEA

Sequences:

>Translated_375_residues
MAHAYRFGIEEELFLANSRTRAAPSESVSAFHKAARSRLEGVEREMLESQAEICSKPSTSFDETHEALAKLRTGLCGIGR
EHGLKVFAAGTHPTAIWTDQRETAKVRYRKMIETLQMVGRRSIVSGLHVHVEVPEPDQRIDLINRLMPFLPVLLALSTSS
PFYERHRTGLAGYRLRAYAELPRTGLPELFDGADDFERYVRVMTRAGAVEDATYFWWHIRPSIRYPTLELRVADSCTRLK
DTLAIAALYRCLVRLCVRQPELNARPTGASRGFVMENLWRAQRDGVHAALIDEAAEEAVPVRRLVERVLDLVAEDAAALG
CNAQIAHALTITECGSSADGQIRAYEASLAAGGSERKALSGVIDWLARETANCEA
>Mature_374_residues
AHAYRFGIEEELFLANSRTRAAPSESVSAFHKAARSRLEGVEREMLESQAEICSKPSTSFDETHEALAKLRTGLCGIGRE
HGLKVFAAGTHPTAIWTDQRETAKVRYRKMIETLQMVGRRSIVSGLHVHVEVPEPDQRIDLINRLMPFLPVLLALSTSSP
FYERHRTGLAGYRLRAYAELPRTGLPELFDGADDFERYVRVMTRAGAVEDATYFWWHIRPSIRYPTLELRVADSCTRLKD
TLAIAALYRCLVRLCVRQPELNARPTGASRGFVMENLWRAQRDGVHAALIDEAAEEAVPVRRLVERVLDLVAEDAAALGC
NAQIAHALTITECGSSADGQIRAYEASLAAGGSERKALSGVIDWLARETANCEA

Specific function: ATP-dependent carboxylate-amine ligase [H]

COG id: COG2170

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carboxylate-amine ligase family [H]

Homologues:

Organism=Escherichia coli, GI1786795, Length=326, Percent_Identity=23.3128834355828, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011793
- InterPro:   IPR006336 [H]

Pfam domain/function: PF04107 GCS2 [H]

EC number: NA

Molecular weight: Translated: 41702; Mature: 41571

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHAYRFGIEEELFLANSRTRAAPSESVSAFHKAARSRLEGVEREMLESQAEICSKPSTS
CCCCHHCCCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FDETHEALAKLRTGLCGIGREHGLKVFAAGTHPTAIWTDQRETAKVRYRKMIETLQMVGR
HHHHHHHHHHHHHHHHCCCHHCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
RSIVSGLHVHVEVPEPDQRIDLINRLMPFLPVLLALSTSSPFYERHRTGLAGYRLRAYAE
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHH
LPRTGLPELFDGADDFERYVRVMTRAGAVEDATYFWWHIRPSIRYPTLELRVADSCTRLK
CCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEECHHHHHHH
DTLAIAALYRCLVRLCVRQPELNARPTGASRGFVMENLWRAQRDGVHAALIDEAAEEAVP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VRRLVERVLDLVAEDAAALGCNAQIAHALTITECGSSADGQIRAYEASLAAGGSERKALS
HHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCCCCCEEEEEHHHHCCCCHHHHHH
GVIDWLARETANCEA
HHHHHHHHHCCCCCC
>Mature Secondary Structure 
AHAYRFGIEEELFLANSRTRAAPSESVSAFHKAARSRLEGVEREMLESQAEICSKPSTS
CCCHHCCCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FDETHEALAKLRTGLCGIGREHGLKVFAAGTHPTAIWTDQRETAKVRYRKMIETLQMVGR
HHHHHHHHHHHHHHHHCCCHHCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
RSIVSGLHVHVEVPEPDQRIDLINRLMPFLPVLLALSTSSPFYERHRTGLAGYRLRAYAE
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHH
LPRTGLPELFDGADDFERYVRVMTRAGAVEDATYFWWHIRPSIRYPTLELRVADSCTRLK
CCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEECHHHHHHH
DTLAIAALYRCLVRLCVRQPELNARPTGASRGFVMENLWRAQRDGVHAALIDEAAEEAVP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VRRLVERVLDLVAEDAAALGCNAQIAHALTITECGSSADGQIRAYEASLAAGGSERKALS
HHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCCCCCEEEEEHHHHCCCCHHHHHH
GVIDWLARETANCEA
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA