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The map label for this gene is ilvE [H]

Identifier: 218532940

GI number: 218532940

Start: 5416525

End: 5417391

Strand: Reverse

Name: ilvE [H]

Synonym: Mchl_5063

Alternate gene names: 218532940

Gene position: 5417391-5416525 (Counterclockwise)

Preceding gene: 218532941

Following gene: 218532937

Centisome position: 93.76

GC content: 69.67

Gene sequence:

>867_bases
ATGACGACTCCCGATCAAGATACCTGGACCTTCTTCGAGGGCGCGTGGCACCCCGGTAACGTCCGGATCATGGGGCCGCG
CACCCACGGTGCGTGGCTTGGCTCGACGGTGTTCGATGGCGCCCGCGCCTTCGAGGGCGTCACGCCCGACCTCGACCTGC
ACCTCGCCCGGGTCAACCGCTCGGCCACCGCCCTCGGTCTCACGCCGAAGGTCGCGGTCGAGGAGTGGGCCCGGCTCGTC
GCCGAGGGGCTGACGCGCTTTGCGCCCGACGCCGAACTCTACATCCGGCCGATGTACTGGGCCGAGAGCGGGTTTGCCGG
GGGCGTGCGCTTCGATCCCGCCTCCACCAACTGGTGCCTGTCGCTCTACACGGCACCGATGCCGCTGCCGTCGGGCGTCA
AGGCGACGCTCTCGCCCTTCCGCCGCCCGTCGATCGAGGTCGCCCCCGTCGATGCCAAGGCCGGCTGCCTCTACCCCAAC
GGGGCGCGGGCGCTCACCGAGGCTTTATCGCGCGGCTTCGGCAATTGCCTGATGCTCGACGGGCTCGGCAACGTCGCCGA
GTTCGCCAACGCCAATGCGTTCTTCGCGCGGGACGGGGTGGTCTACACCCCGGTCCCGAACGGGACGTTCCTCGCCGGCA
TCACCCGCGCCCGCGTCGTGGCGCTGTTGCGCGGGGCCGGTGTCACGGTGGTGGAGAAGACGCTGCGCTACGAGGACTTC
CTGAGCGCCGACGAGGTCTTCACCGCCGGCAACTTCGCCAAGGTCGCCCCGGTCACCGGGCTCGATGACGTACACTTCGA
GCCAGGGCCGGTGTTTCGCCACGCACGCCAGCTTTACTGGGATTTCGCGCACGGACGGCTGTCGTAG

Upstream 100 bases:

>100_bases
TGCGCGCGGCCGGCAAGGCCAAGCGGATGCTGAGCCGCCGCTCCGGCTCCGGCAGCGCGCTCGAAGCCGCCTGAATGATA
CCCTCGAAGGCTCGGCCGCG

Downstream 100 bases:

>100_bases
CGGCACAAAGCTCAGGCTTTGCAGCCGCCACAGACTCATCTCGGTCAGCAGCTCAGACCGCGGCGCTCACGGCGACCGAA
GGTCTGGTAGTGCGCAGCTC

Product: aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTTPDQDTWTFFEGAWHPGNVRIMGPRTHGAWLGSTVFDGARAFEGVTPDLDLHLARVNRSATALGLTPKVAVEEWARLV
AEGLTRFAPDAELYIRPMYWAESGFAGGVRFDPASTNWCLSLYTAPMPLPSGVKATLSPFRRPSIEVAPVDAKAGCLYPN
GARALTEALSRGFGNCLMLDGLGNVAEFANANAFFARDGVVYTPVPNGTFLAGITRARVVALLRGAGVTVVEKTLRYEDF
LSADEVFTAGNFAKVAPVTGLDDVHFEPGPVFRHARQLYWDFAHGRLS

Sequences:

>Translated_288_residues
MTTPDQDTWTFFEGAWHPGNVRIMGPRTHGAWLGSTVFDGARAFEGVTPDLDLHLARVNRSATALGLTPKVAVEEWARLV
AEGLTRFAPDAELYIRPMYWAESGFAGGVRFDPASTNWCLSLYTAPMPLPSGVKATLSPFRRPSIEVAPVDAKAGCLYPN
GARALTEALSRGFGNCLMLDGLGNVAEFANANAFFARDGVVYTPVPNGTFLAGITRARVVALLRGAGVTVVEKTLRYEDF
LSADEVFTAGNFAKVAPVTGLDDVHFEPGPVFRHARQLYWDFAHGRLS
>Mature_287_residues
TTPDQDTWTFFEGAWHPGNVRIMGPRTHGAWLGSTVFDGARAFEGVTPDLDLHLARVNRSATALGLTPKVAVEEWARLVA
EGLTRFAPDAELYIRPMYWAESGFAGGVRFDPASTNWCLSLYTAPMPLPSGVKATLSPFRRPSIEVAPVDAKAGCLYPNG
ARALTEALSRGFGNCLMLDGLGNVAEFANANAFFARDGVVYTPVPNGTFLAGITRARVVALLRGAGVTVVEKTLRYEDFL
SADEVFTAGNFAKVAPVTGLDDVHFEPGPVFRHARQLYWDFAHGRLS

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=295, Percent_Identity=27.4576271186441, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 31209; Mature: 31077

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPDQDTWTFFEGAWHPGNVRIMGPRTHGAWLGSTVFDGARAFEGVTPDLDLHLARVNR
CCCCCCCCEEEEECCCCCCCEEEECCCCCCCHHCHHHHHHHHHHCCCCCCCEEEEEECCC
SATALGLTPKVAVEEWARLVAEGLTRFAPDAELYIRPMYWAESGFAGGVRFDPASTNWCL
CCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEECCCCCCEEE
SLYTAPMPLPSGVKATLSPFRRPSIEVAPVDAKAGCLYPNGARALTEALSRGFGNCLMLD
EEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEC
GLGNVAEFANANAFFARDGVVYTPVPNGTFLAGITRARVVALLRGAGVTVVEKTLRYEDF
CCCCHHHHCCCCEEEECCCEEEECCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LSADEVFTAGNFAKVAPVTGLDDVHFEPGPVFRHARQLYWDFAHGRLS
CCHHHEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTPDQDTWTFFEGAWHPGNVRIMGPRTHGAWLGSTVFDGARAFEGVTPDLDLHLARVNR
CCCCCCCEEEEECCCCCCCEEEECCCCCCCHHCHHHHHHHHHHCCCCCCCEEEEEECCC
SATALGLTPKVAVEEWARLVAEGLTRFAPDAELYIRPMYWAESGFAGGVRFDPASTNWCL
CCEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEECCCCCCEEE
SLYTAPMPLPSGVKATLSPFRRPSIEVAPVDAKAGCLYPNGARALTEALSRGFGNCLMLD
EEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEC
GLGNVAEFANANAFFARDGVVYTPVPNGTFLAGITRARVVALLRGAGVTVVEKTLRYEDF
CCCCHHHHCCCCEEEECCCEEEECCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LSADEVFTAGNFAKVAPVTGLDDVHFEPGPVFRHARQLYWDFAHGRLS
CCHHHEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]