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The map label for this gene is aes [H]

Identifier: 218532933

GI number: 218532933

Start: 5409698

End: 5410615

Strand: Reverse

Name: aes [H]

Synonym: Mchl_5056

Alternate gene names: 218532933

Gene position: 5410615-5409698 (Counterclockwise)

Preceding gene: 218532934

Following gene: 218532932

Centisome position: 93.64

GC content: 74.84

Gene sequence:

>918_bases
ATGCCGCTCGATCCGCATGTCAGCCGCTTCCTCGACATGATGGCGCTCGGAAGCTCGGACGGCATCACCGTCGACGCCCG
CCGGGAGGGGATGCGCGGGCTCGCGCGCCTCGCCGGGCTGAGCGCCGAGGGCGTCGCGACCCGCGATCTCGTCCTGCCGG
GTCCGGCCGGGCCGATTTCCGCGCGGCTCTACGCGCCGGAGGGCAGCGCAAGTGGACCCGGTCTCGTCTACCTGCACGGC
GGCGGCCTCGTGACCGGCGGCCTCGACACCCATGACGGACTCTGCCGAGTGCTGGCCGAGGCCGGCGCCCTGCGCGTCGT
CTCGGTCGATTACCGGCTCGCGCCCGAACACCCGTTCCCGGCCGCCCTCGACGACGCGCTGGCGGCGATGGATGGGGTCG
CCGCACAGGCGGACGCGCTCCGGATCGATCCGGCCCGGCTCGGGATCGGCGGCGATTCCGCGGGGGCCGGGCTCGCCGCA
CGGGTCTGCCAGGAGCGGCGCGGCGGCGCGATCCGGGCGCAGCTCCTGATCTGCCCCGTGCTCGACGTGCACGGCAGCCA
CCCCTCGCGCCGCGATTTCGCTCAAGGATACTTCCTCGATGAGGCGACGATCCGGCGCGACCTTGCGGCCTGCGGCCCCG
AGGCCGCCGGCCCGTCGCCCCGCCTCTCGCCCCTGCGCGAGGCCGATCTGACCGGCCTGCCCCCGGCCCGCATCCACATG
GCGGAATACGACCCCGTCCGCGACGAAGGCGCCGCCTATGCCGCGCGTCTGGAGCGGGCGGGCGTCGAGACCGCCCACAC
CGTTCACGCCGGCATGATCCACTTCTTCTACGGCCTCGGGCGGCTGGTGCCGCGCGGCCGGCCGATCCTGTCCGAGATCG
CCCGCGACTTCGGCGCCTGCCTCCGCGGCGCCTCCTAA

Upstream 100 bases:

>100_bases
GCCTCGCGGTGGCCCTGTTCGCCACCCACGGCGAGAGCATCGACGCGTGGCCGGCCCCGGTCCGCGCGGCGCTGAAGCTC
TGACGTCAAGGCGCCGTCCG

Downstream 100 bases:

>100_bases
CCCCACCCCTTGTCCACCCCTTGCCCAACCCAGCCTTGAGGAGAGCCACCATGTCCCTGCGTGAGACCGTCTTCGCCGAG
ATCGCCGCCATCGCCGCCGA

Product: alpha/beta hydrolase fold-3 domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MPLDPHVSRFLDMMALGSSDGITVDARREGMRGLARLAGLSAEGVATRDLVLPGPAGPISARLYAPEGSASGPGLVYLHG
GGLVTGGLDTHDGLCRVLAEAGALRVVSVDYRLAPEHPFPAALDDALAAMDGVAAQADALRIDPARLGIGGDSAGAGLAA
RVCQERRGGAIRAQLLICPVLDVHGSHPSRRDFAQGYFLDEATIRRDLAACGPEAAGPSPRLSPLREADLTGLPPARIHM
AEYDPVRDEGAAYAARLERAGVETAHTVHAGMIHFFYGLGRLVPRGRPILSEIARDFGACLRGAS

Sequences:

>Translated_305_residues
MPLDPHVSRFLDMMALGSSDGITVDARREGMRGLARLAGLSAEGVATRDLVLPGPAGPISARLYAPEGSASGPGLVYLHG
GGLVTGGLDTHDGLCRVLAEAGALRVVSVDYRLAPEHPFPAALDDALAAMDGVAAQADALRIDPARLGIGGDSAGAGLAA
RVCQERRGGAIRAQLLICPVLDVHGSHPSRRDFAQGYFLDEATIRRDLAACGPEAAGPSPRLSPLREADLTGLPPARIHM
AEYDPVRDEGAAYAARLERAGVETAHTVHAGMIHFFYGLGRLVPRGRPILSEIARDFGACLRGAS
>Mature_304_residues
PLDPHVSRFLDMMALGSSDGITVDARREGMRGLARLAGLSAEGVATRDLVLPGPAGPISARLYAPEGSASGPGLVYLHGG
GLVTGGLDTHDGLCRVLAEAGALRVVSVDYRLAPEHPFPAALDDALAAMDGVAAQADALRIDPARLGIGGDSAGAGLAAR
VCQERRGGAIRAQLLICPVLDVHGSHPSRRDFAQGYFLDEATIRRDLAACGPEAAGPSPRLSPLREADLTGLPPARIHMA
EYDPVRDEGAAYAARLERAGVETAHTVHAGMIHFFYGLGRLVPRGRPILSEIARDFGACLRGAS

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=294, Percent_Identity=29.2517006802721, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI157041239, Length=132, Percent_Identity=40.9090909090909, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI68299767, Length=280, Percent_Identity=28.2142857142857, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI61966717, Length=132, Percent_Identity=34.0909090909091, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI157041237, Length=99, Percent_Identity=38.3838383838384, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786682, Length=287, Percent_Identity=30.3135888501742, Blast_Score=104, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17567059, Length=144, Percent_Identity=38.1944444444444, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI72001146, Length=133, Percent_Identity=36.8421052631579, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=130, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 31753; Mature: 31621

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDPHVSRFLDMMALGSSDGITVDARREGMRGLARLAGLSAEGVATRDLVLPGPAGPIS
CCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCE
ARLYAPEGSASGPGLVYLHGGGLVTGGLDTHDGLCRVLAEAGALRVVSVDYRLAPEHPFP
EEEECCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCC
AALDDALAAMDGVAAQADALRIDPARLGIGGDSAGAGLAARVCQERRGGAIRAQLLICPV
HHHHHHHHHHHCHHHCCCCEEECHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEH
LDVHGSHPSRRDFAQGYFLDEATIRRDLAACGPEAAGPSPRLSPLREADLTGLPPARIHM
HHCCCCCCCHHHHHCCEEECHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCHHEEEE
AEYDPVRDEGAAYAARLERAGVETAHTVHAGMIHFFYGLGRLVPRGRPILSEIARDFGAC
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LRGAS
HCCCC
>Mature Secondary Structure 
PLDPHVSRFLDMMALGSSDGITVDARREGMRGLARLAGLSAEGVATRDLVLPGPAGPIS
CCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCE
ARLYAPEGSASGPGLVYLHGGGLVTGGLDTHDGLCRVLAEAGALRVVSVDYRLAPEHPFP
EEEECCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCC
AALDDALAAMDGVAAQADALRIDPARLGIGGDSAGAGLAARVCQERRGGAIRAQLLICPV
HHHHHHHHHHHCHHHCCCCEEECHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEH
LDVHGSHPSRRDFAQGYFLDEATIRRDLAACGPEAAGPSPRLSPLREADLTGLPPARIHM
HHCCCCCCCHHHHHCCEEECHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCHHEEEE
AEYDPVRDEGAAYAARLERAGVETAHTVHAGMIHFFYGLGRLVPRGRPILSEIARDFGAC
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LRGAS
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA