Click here to switch to the map view.

The map label for this gene is bepF [H]

Identifier: 218532849

GI number: 218532849

Start: 5314402

End: 5315547

Strand: Reverse

Name: bepF [H]

Synonym: Mchl_4972

Alternate gene names: 218532849

Gene position: 5315547-5314402 (Counterclockwise)

Preceding gene: 218532850

Following gene: 218532848

Centisome position: 92.0

GC content: 67.63

Gene sequence:

>1146_bases
ATGATGCGTAGCCTCAGGCCTCTTCTTCTCGCCACACCGCTGATCGCCTCCGCGCTGCTGGGTGCCGGGCCGGCCGGCGC
GCAGATGCCCGGCGCGCCGCCGCCGGGCGTGACCGTCGCTAAGCCGGTGGTGCGGGAGATCGTCGAGCAGGACCAGTATA
CCGGCCGGTTCGACCCGATCGAATTCGTCGAGGTCCGCGCCCGCGTGACCGGCTACCTCGACAAGATCATGTTCCAGGAC
GGCGCGAACGTGAAGAAGGGCGAAGTGCTCTTCATCATCGACCGCCGCCCCTACAAGGCGGCCTTGGAGCAGGCCCAGGC
CGCGCTCACCTCGGCCAAGGCGCGGCTCTCCTTCACCCAGACGGATCTGGAGCGTGCCCAGGTGCTGTCGCGCGGCGGCA
ACATCTCCGAGCAGGTCACCGACCAGCGCCGCCAGGCCTCGCAGACCGCCCAAGCCGACGTCGACAGCGCGGAAGCGGCG
CTTCGCAACGCGCAGCTCAACTACGACTTCACGGAAGTGAAATCGCCGATCAACGGGCGGATCAGCCAGCGTCTCGTCAC
CGAGGGCAACATCGTCAGCACCGACCAGACGATCCTGGCCCGGATCGTCTCGCTCGATCCGGTCTATTTCGGCTTCACCG
TCGATGAGCGCTCGTTCCTGAAGTACCAGGGCTCGCTCGGCATCGGCATGGGCCAGATGCAGCGCGGCAAGGGCGCCCCG
GTGCAGATCGCCCTGTCCGGCGAGGAGAAGCCGACCCGCAAGGGCACGCTCGACTTCGTCGACAACCGTGTCGACAACCA
GACCGGCACGATGCTCCTGCGCGCCACCGTCGAGAACCCGGACAACTTCATCAAGCCGGGCCTGTTCGGCATCGTCGCGA
TGCCCGCGACCAAGCCGTTCCAGGGCATCCTGATCCCCGACGAGGCGGTCTCGGCCAACCAGGACAAGCGCATCGTCTAC
GTGCTCGGCGATGGCGACAAGGTCTCGGCCAGGACCGTGAAGCTCGGGCCGATCGTCGACGGCTACCGCGTGGTCCGCGA
CGGCCTGAAGGGCGACGAACTCGTCGTGGTCAACGGCGTCGCCAAGGTCCGCCCGGGCGCGCAGGTGAAGCCCGAGACGG
TCGAGCTGCCGCCGTCGAAGAAGTGA

Upstream 100 bases:

>100_bases
CAAAGCGTCGAACCGTTCCTTTCGTGACGGACCGTCATGTGCGGGAGCGCACGCCCCACTCGAGGTTGCGGGGACTAGGC
CGCGCCATGCAGGGAACCGA

Downstream 100 bases:

>100_bases
CGTCGCGGTGATGCGGCACCGTCGCGGGGGCGAGGCGGGACGCGGACAGTAAAGATTTTCGAGCGCCGACGCTCCCGGAC
CGACCGGGGATGCCGGCCTG

Product: efflux transporter RND family, MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MMRSLRPLLLATPLIASALLGAGPAGAQMPGAPPPGVTVAKPVVREIVEQDQYTGRFDPIEFVEVRARVTGYLDKIMFQD
GANVKKGEVLFIIDRRPYKAALEQAQAALTSAKARLSFTQTDLERAQVLSRGGNISEQVTDQRRQASQTAQADVDSAEAA
LRNAQLNYDFTEVKSPINGRISQRLVTEGNIVSTDQTILARIVSLDPVYFGFTVDERSFLKYQGSLGIGMGQMQRGKGAP
VQIALSGEEKPTRKGTLDFVDNRVDNQTGTMLLRATVENPDNFIKPGLFGIVAMPATKPFQGILIPDEAVSANQDKRIVY
VLGDGDKVSARTVKLGPIVDGYRVVRDGLKGDELVVVNGVAKVRPGAQVKPETVELPPSKK

Sequences:

>Translated_381_residues
MMRSLRPLLLATPLIASALLGAGPAGAQMPGAPPPGVTVAKPVVREIVEQDQYTGRFDPIEFVEVRARVTGYLDKIMFQD
GANVKKGEVLFIIDRRPYKAALEQAQAALTSAKARLSFTQTDLERAQVLSRGGNISEQVTDQRRQASQTAQADVDSAEAA
LRNAQLNYDFTEVKSPINGRISQRLVTEGNIVSTDQTILARIVSLDPVYFGFTVDERSFLKYQGSLGIGMGQMQRGKGAP
VQIALSGEEKPTRKGTLDFVDNRVDNQTGTMLLRATVENPDNFIKPGLFGIVAMPATKPFQGILIPDEAVSANQDKRIVY
VLGDGDKVSARTVKLGPIVDGYRVVRDGLKGDELVVVNGVAKVRPGAQVKPETVELPPSKK
>Mature_381_residues
MMRSLRPLLLATPLIASALLGAGPAGAQMPGAPPPGVTVAKPVVREIVEQDQYTGRFDPIEFVEVRARVTGYLDKIMFQD
GANVKKGEVLFIIDRRPYKAALEQAQAALTSAKARLSFTQTDLERAQVLSRGGNISEQVTDQRRQASQTAQADVDSAEAA
LRNAQLNYDFTEVKSPINGRISQRLVTEGNIVSTDQTILARIVSLDPVYFGFTVDERSFLKYQGSLGIGMGQMQRGKGAP
VQIALSGEEKPTRKGTLDFVDNRVDNQTGTMLLRATVENPDNFIKPGLFGIVAMPATKPFQGILIPDEAVSANQDKRIVY
VLGDGDKVSARTVKLGPIVDGYRVVRDGLKGDELVVVNGVAKVRPGAQVKPETVELPPSKK

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=375, Percent_Identity=34.4, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789929, Length=369, Percent_Identity=29.5392953929539, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789665, Length=377, Percent_Identity=30.7692307692308, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87082036, Length=323, Percent_Identity=30.9597523219814, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87081951, Length=208, Percent_Identity=25.9615384615385, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41227; Mature: 41227

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRSLRPLLLATPLIASALLGAGPAGAQMPGAPPPGVTVAKPVVREIVEQDQYTGRFDPI
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCH
EFVEVRARVTGYLDKIMFQDGANVKKGEVLFIIDRRPYKAALEQAQAALTSAKARLSFTQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEEHH
TDLERAQVLSRGGNISEQVTDQRRQASQTAQADVDSAEAALRNAQLNYDFTEVKSPINGR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHCCCCCCH
ISQRLVTEGNIVSTDQTILARIVSLDPVYFGFTVDERSFLKYQGSLGIGMGQMQRGKGAP
HHHHEECCCCEEECCHHHHHHHHHCCCEEEEEEECCCHHEEECCCCCCCCCHHCCCCCCC
VQIALSGEEKPTRKGTLDFVDNRVDNQTGTMLLRATVENPDNFIKPGLFGIVAMPATKPF
EEEEECCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEECCCCHHHCCCCEEEEECCCCCCC
QGILIPDEAVSANQDKRIVYVLGDGDKVSARTVKLGPIVDGYRVVRDGLKGDELVVVNGV
CEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCHHHHHHCCCCCCCEEEEECH
AKVRPGAQVKPETVELPPSKK
HHCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure
MMRSLRPLLLATPLIASALLGAGPAGAQMPGAPPPGVTVAKPVVREIVEQDQYTGRFDPI
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCH
EFVEVRARVTGYLDKIMFQDGANVKKGEVLFIIDRRPYKAALEQAQAALTSAKARLSFTQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEEHH
TDLERAQVLSRGGNISEQVTDQRRQASQTAQADVDSAEAALRNAQLNYDFTEVKSPINGR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHCCCCCCH
ISQRLVTEGNIVSTDQTILARIVSLDPVYFGFTVDERSFLKYQGSLGIGMGQMQRGKGAP
HHHHEECCCCEEECCHHHHHHHHHCCCEEEEEEECCCHHEEECCCCCCCCCHHCCCCCCC
VQIALSGEEKPTRKGTLDFVDNRVDNQTGTMLLRATVENPDNFIKPGLFGIVAMPATKPF
EEEEECCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEECCCCHHHCCCCEEEEECCCCCCC
QGILIPDEAVSANQDKRIVYVLGDGDKVSARTVKLGPIVDGYRVVRDGLKGDELVVVNGV
CEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCHHHHHHCCCCCCCEEEEECH
AKVRPGAQVKPETVELPPSKK
HHCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]