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The map label for this gene is folC [H]

Identifier: 218532785

GI number: 218532785

Start: 5229688

End: 5231013

Strand: Reverse

Name: folC [H]

Synonym: Mchl_4908

Alternate gene names: 218532785

Gene position: 5231013-5229688 (Counterclockwise)

Preceding gene: 218532786

Following gene: 218532784

Centisome position: 90.53

GC content: 70.81

Gene sequence:

>1326_bases
ATGGAATCCTCCGACGCGTTGATGGCGCGCTTCCTCGCCCTGCATCCGCGCACGATCGACCTGTCGCTCGGGCGCATCCA
GCGCCTGCTCGCGGCCCTGAATCATCCCGAGCGGCGGCTGCCGCCGGTGATTCACGTCGCCGGCACCAACGGCAAGGGCT
CGACCATCGCCTTCATGCGGGCAATCCTGGAGGCGGGGGGCCTAGCCGCCCACGTCTACACCTCGCCCCACCTCGTGCGC
TTCCACGAGCGCATCCGCCTCGGCGGCATCGGCGGCGGTCACTACGTCGCCGAAGACCGGCTCGCCGACGCCTTCGCCCG
GTGCGAGGCGGCCAACAAGGGCGATCCGATCACCGTGTTCGAGATCACCACCGCGGCCGCCCTGCTGCTGTTTTCCGAAT
GCCCCGCCGACGTGCTGCTGCTGGAAGTGGGCCTCGGCGGCCGGGTCGATGCCACCAATGTCATCGACCACCCGGCCTGC
GCCGTGGTCACCCCGATCGGGCGCGACCATGCCGAATATCTCGGCGACACCGTCGAGGCGGTGGCGATGGAGAAGGCCGG
CATCTTCAAGCGCGGCTGCCCGGCGGTGATCGCCGCCCAGGATTATGCCGGGGCCGATGCCGTCCTCTGCCGCCAGGCCG
AGCGGATCGGCGCGGTGCCGGTGCGGATCGGCAACCAGGACTTCTCCGTACACGAGGAGAGCGGGCGCTTCGTCTACCAG
GACGAGGTCGACCTGTTCGATCTGCCCCGGCCCCGCCTCGCCGGGCGCCACCAGCTCACCAATGCCGGCACCGCCATCGC
AGCCCTGCGCGCGGCGGGCTTCGGCGATATCGGGACGGTCGCCCTCGAAGCCGGCCTGCGCAATGTCGATTGGCCGGGCC
GGCTCCAGCGCCTCGTGCGCGGGGCGCTCGCCGAGCGGATGCCCAAGGACGCCGAGCTGTGGCTCGACGGCGGCCACAAC
GCCGATGGCGGGCGGATCCTCGCCGCCGCCATGGCCGATCTCGGCGAGCGCAGCGACGTGCCGCTGGTCCTGATTGTCGG
GCTGCTCGGCACCAAGGATGCCGAAGGCTTCTTGAAGAACTTCGTCGGCCTTGCCCGCTCACTTGTGGCGGTGCCGATCA
CCGGCCAGATGGCGGCGCGGCCCGCCGAGGAAGTGGCGGAGATCGCCCGTGAGGTCGGTCTCTCGGCCGAGGTCGCTCCG
AGCGTCGAGGCGGCGTTGGCGGCCCTGTCGGACACGGTCTTCGAGCGCCCGCCGCGAGTCCTCATCTGCGGTTCGCTTTA
TCTCGCGGGCGCCGTGCTCGAAGCCAACGGCACGATCCCGGTCTGA

Upstream 100 bases:

>100_bases
GCCGGACGCCCCAACCCGGTACCGCCCCCGAGCCGACCACACCCGAACCCCTGCCGAACGCGGCCTGAACTCAATCCGCG
ACAGAAGGTCTCCGCGCGAG

Downstream 100 bases:

>100_bases
CCGCATCCGCCGGATCGAAGCGGACATGCGACGGGCGCGGCGCAAGGGGCAGAGAATCGCGCCGCAAGGCCATCGGTGGG
CAAACCACACGAGCCGGAAG

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MESSDALMARFLALHPRTIDLSLGRIQRLLAALNHPERRLPPVIHVAGTNGKGSTIAFMRAILEAGGLAAHVYTSPHLVR
FHERIRLGGIGGGHYVAEDRLADAFARCEAANKGDPITVFEITTAAALLLFSECPADVLLLEVGLGGRVDATNVIDHPAC
AVVTPIGRDHAEYLGDTVEAVAMEKAGIFKRGCPAVIAAQDYAGADAVLCRQAERIGAVPVRIGNQDFSVHEESGRFVYQ
DEVDLFDLPRPRLAGRHQLTNAGTAIAALRAAGFGDIGTVALEAGLRNVDWPGRLQRLVRGALAERMPKDAELWLDGGHN
ADGGRILAAAMADLGERSDVPLVLIVGLLGTKDAEGFLKNFVGLARSLVAVPITGQMAARPAEEVAEIAREVGLSAEVAP
SVEAALAALSDTVFERPPRVLICGSLYLAGAVLEANGTIPV

Sequences:

>Translated_441_residues
MESSDALMARFLALHPRTIDLSLGRIQRLLAALNHPERRLPPVIHVAGTNGKGSTIAFMRAILEAGGLAAHVYTSPHLVR
FHERIRLGGIGGGHYVAEDRLADAFARCEAANKGDPITVFEITTAAALLLFSECPADVLLLEVGLGGRVDATNVIDHPAC
AVVTPIGRDHAEYLGDTVEAVAMEKAGIFKRGCPAVIAAQDYAGADAVLCRQAERIGAVPVRIGNQDFSVHEESGRFVYQ
DEVDLFDLPRPRLAGRHQLTNAGTAIAALRAAGFGDIGTVALEAGLRNVDWPGRLQRLVRGALAERMPKDAELWLDGGHN
ADGGRILAAAMADLGERSDVPLVLIVGLLGTKDAEGFLKNFVGLARSLVAVPITGQMAARPAEEVAEIAREVGLSAEVAP
SVEAALAALSDTVFERPPRVLICGSLYLAGAVLEANGTIPV
>Mature_441_residues
MESSDALMARFLALHPRTIDLSLGRIQRLLAALNHPERRLPPVIHVAGTNGKGSTIAFMRAILEAGGLAAHVYTSPHLVR
FHERIRLGGIGGGHYVAEDRLADAFARCEAANKGDPITVFEITTAAALLLFSECPADVLLLEVGLGGRVDATNVIDHPAC
AVVTPIGRDHAEYLGDTVEAVAMEKAGIFKRGCPAVIAAQDYAGADAVLCRQAERIGAVPVRIGNQDFSVHEESGRFVYQ
DEVDLFDLPRPRLAGRHQLTNAGTAIAALRAAGFGDIGTVALEAGLRNVDWPGRLQRLVRGALAERMPKDAELWLDGGHN
ADGGRILAAAMADLGERSDVPLVLIVGLLGTKDAEGFLKNFVGLARSLVAVPITGQMAARPAEEVAEIAREVGLSAEVAP
SVEAALAALSDTVFERPPRVLICGSLYLAGAVLEANGTIPV

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=351, Percent_Identity=31.9088319088319, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI66932990, Length=351, Percent_Identity=31.9088319088319, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI1788654, Length=422, Percent_Identity=34.3601895734597, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71984923, Length=321, Percent_Identity=29.595015576324, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17553148, Length=321, Percent_Identity=29.595015576324, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17553150, Length=322, Percent_Identity=29.5031055900621, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323760, Length=440, Percent_Identity=29.3181818181818, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324815, Length=457, Percent_Identity=25.601750547046, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24641571, Length=155, Percent_Identity=34.1935483870968, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24581568, Length=186, Percent_Identity=31.7204301075269, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46523; Mature: 46523

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSDALMARFLALHPRTIDLSLGRIQRLLAALNHPERRLPPVIHVAGTNGKGSTIAFMR
CCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCHHHHHHH
AILEAGGLAAHVYTSPHLVRFHERIRLGGIGGGHYVAEDRLADAFARCEAANKGDPITVF
HHHHCCCEEEEEECCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
EITTAAALLLFSECPADVLLLEVGLGGRVDATNVIDHPACAVVTPIGRDHAEYLGDTVEA
EHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHH
VAMEKAGIFKRGCPAVIAAQDYAGADAVLCRQAERIGAVPVRIGNQDFSVHEESGRFVYQ
HHHHHHCCHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCEEECCCCCEEEE
DEVDLFDLPRPRLAGRHQLTNAGTAIAALRAAGFGDIGTVALEAGLRNVDWPGRLQRLVR
CCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHH
GALAERMPKDAELWLDGGHNADGGRILAAAMADLGERSDVPLVLIVGLLGTKDAEGFLKN
HHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEHHCCCCHHHHHHH
FVGLARSLVAVPITGQMAARPAEEVAEIAREVGLSAEVAPSVEAALAALSDTVFERPPRV
HHHHHHHHEEECCCCHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCE
LICGSLYLAGAVLEANGTIPV
EEECCHHHHCEEEECCCCCCC
>Mature Secondary Structure
MESSDALMARFLALHPRTIDLSLGRIQRLLAALNHPERRLPPVIHVAGTNGKGSTIAFMR
CCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCHHHHHHH
AILEAGGLAAHVYTSPHLVRFHERIRLGGIGGGHYVAEDRLADAFARCEAANKGDPITVF
HHHHCCCEEEEEECCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
EITTAAALLLFSECPADVLLLEVGLGGRVDATNVIDHPACAVVTPIGRDHAEYLGDTVEA
EHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHH
VAMEKAGIFKRGCPAVIAAQDYAGADAVLCRQAERIGAVPVRIGNQDFSVHEESGRFVYQ
HHHHHHCCHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCEEECCCCCEEEE
DEVDLFDLPRPRLAGRHQLTNAGTAIAALRAAGFGDIGTVALEAGLRNVDWPGRLQRLVR
CCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHH
GALAERMPKDAELWLDGGHNADGGRILAAAMADLGERSDVPLVLIVGLLGTKDAEGFLKN
HHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEHHCCCCHHHHHHH
FVGLARSLVAVPITGQMAARPAEEVAEIAREVGLSAEVAPSVEAALAALSDTVFERPPRV
HHHHHHHHEEECCCCHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCE
LICGSLYLAGAVLEANGTIPV
EEECCHHHHCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]