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The map label for this gene is ywnE [H]

Identifier: 218532779

GI number: 218532779

Start: 5217927

End: 5219363

Strand: Reverse

Name: ywnE [H]

Synonym: Mchl_4902

Alternate gene names: 218532779

Gene position: 5219363-5217927 (Counterclockwise)

Preceding gene: 218532782

Following gene: 218532776

Centisome position: 90.33

GC content: 68.89

Gene sequence:

>1437_bases
GTGGAAGAAGTCCTCACTCGATGGCTCGGCACGACGGCCTCGCTCCGCACCGAGATCGTCGCGGCCATCGGCCTGGTGCT
CACATTCGGCGTCAGCGTTCACGTGCTGCTGCGCAAGCGGGTGGTGGGCGCGGCGATCGGCTGGATCGGACTGGCGTGGC
TGGCGCCGGTCTTCGGCACGCTGCTCTACCTCACCTTCGGCATCAACCGGGTGGAGCGGCGCGCCCGCCGGCTGAAGCGG
CGCCCCTCGCAGACCATCGACGAGACGCCGCAGCCCTCCGCCCACGTGCCCGAGGCCTACCACGCCCTCGACTTGGCGGT
GCAGGCGGTGACCGGCCTGCCGACGGTGGCGGGCAATCGGTTCGAGCTCCTCCGCAACGGCGACGAGGCCTATCCGGCGA
TGCTGGCGGCGATCGGCGGGGCCCGCCGCAGCGTCGCCCTGTCGAGCTATATCTTTCGCGACGACGCCACCGGCCGGGCG
TTCTGCGCCGCGTTGAAGGCGGCACAGGATCGCGGCGCCGAGGTGCGCGTGATCCTCGACGGCATCGGCAGCGGCTATTT
CTTCCCGGCCGCGTGGCGGCATCTGCGCCGGCTCGGGGTGCCGGCCGGCCTGTTCATGCACTCCGTCCTGCCCTGGCGGA
TGCCGTTCCTGAACCTGCGCACGCACAAGAAGCTGCTGATCGTCGACGGGAGGGTCGCCTTCACCGGCGGCGTCAACATC
GCCGACGGCAACCGGGTCTCGGAGAAGCCGGACTTTCCGATCCGCGACACGCATTTCCGGATCGAGGGCCCGGTGGTCGA
GCACCTGACCCAGGCCTTCATCGCCGATTGGCTGTTCGTCAGCGACGAGGAGCTGGAGGGCGAGGCGTGGCTGCCGCGGT
TGGCGCCGGTTGGCGCGGTCACCGCCCGCGTCGTCACCTCCGGCCCGGACGCCGACATCGAGAAGATCGCAACCGTGGTG
CTCCAGGCGATTGCCTGCGCGAAGGAGTCGATCCGGCTGACGACCCCCTATTTCCTGCCCAACGAACTCGTGCTGAACGC
GCTGAGCCTCGCGGCGGCGCGGGGGATCGCGGTCGATATCGCCATCCCGCGCAAGAGCGACCACCGCTTTGTCGATTGGG
CGACCCACGGCCATATCGATCCGCTCCTGAAGAGCGGCGTGCGGGTCTGGATCGACGAGCCGCCCTTCGATCACTCGAAG
GCGATGACCGTCGATGGGCAATGGTGCTTCATCGGCAGCGCCAACTGGGATTCGCGCAGCTTCCGCCTGAACTTCGAGCT
CAACGTGGAGGTCTACGACGTGGCGCTGGCCCGGGCGCTCGAAGCCTTCATTGCGACGAAGTTTCAGACCCCTTTGCGCT
TGGCCGACCTCGACGCCCGCTCGCTCCCCGTGCGCCTGCGCGACGCAGCCGTCCGACTGCTTCTGCCATACCTCTGA

Upstream 100 bases:

>100_bases
AGCATGCAACGGCGATTTGTGGAGGGCTGCCTGTTCGTCACACCAGGCTTGGCTTATCTCCTGCGCCGTTCCCGGACAGG
GCAGTGCAGCAGGCGGACCG

Downstream 100 bases:

>100_bases
GCCGGCCGACCGAAGGGACCCCCTCCGGCGCGGGCGAGAGGTGCACCACGATCGGGTGATGGTCGGAGAGCCCGCGGGGC
AGGACCCTACGCTCGTGGCA

Product: phospholipase D/transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MEEVLTRWLGTTASLRTEIVAAIGLVLTFGVSVHVLLRKRVVGAAIGWIGLAWLAPVFGTLLYLTFGINRVERRARRLKR
RPSQTIDETPQPSAHVPEAYHALDLAVQAVTGLPTVAGNRFELLRNGDEAYPAMLAAIGGARRSVALSSYIFRDDATGRA
FCAALKAAQDRGAEVRVILDGIGSGYFFPAAWRHLRRLGVPAGLFMHSVLPWRMPFLNLRTHKKLLIVDGRVAFTGGVNI
ADGNRVSEKPDFPIRDTHFRIEGPVVEHLTQAFIADWLFVSDEELEGEAWLPRLAPVGAVTARVVTSGPDADIEKIATVV
LQAIACAKESIRLTTPYFLPNELVLNALSLAAARGIAVDIAIPRKSDHRFVDWATHGHIDPLLKSGVRVWIDEPPFDHSK
AMTVDGQWCFIGSANWDSRSFRLNFELNVEVYDVALARALEAFIATKFQTPLRLADLDARSLPVRLRDAAVRLLLPYL

Sequences:

>Translated_478_residues
MEEVLTRWLGTTASLRTEIVAAIGLVLTFGVSVHVLLRKRVVGAAIGWIGLAWLAPVFGTLLYLTFGINRVERRARRLKR
RPSQTIDETPQPSAHVPEAYHALDLAVQAVTGLPTVAGNRFELLRNGDEAYPAMLAAIGGARRSVALSSYIFRDDATGRA
FCAALKAAQDRGAEVRVILDGIGSGYFFPAAWRHLRRLGVPAGLFMHSVLPWRMPFLNLRTHKKLLIVDGRVAFTGGVNI
ADGNRVSEKPDFPIRDTHFRIEGPVVEHLTQAFIADWLFVSDEELEGEAWLPRLAPVGAVTARVVTSGPDADIEKIATVV
LQAIACAKESIRLTTPYFLPNELVLNALSLAAARGIAVDIAIPRKSDHRFVDWATHGHIDPLLKSGVRVWIDEPPFDHSK
AMTVDGQWCFIGSANWDSRSFRLNFELNVEVYDVALARALEAFIATKFQTPLRLADLDARSLPVRLRDAAVRLLLPYL
>Mature_478_residues
MEEVLTRWLGTTASLRTEIVAAIGLVLTFGVSVHVLLRKRVVGAAIGWIGLAWLAPVFGTLLYLTFGINRVERRARRLKR
RPSQTIDETPQPSAHVPEAYHALDLAVQAVTGLPTVAGNRFELLRNGDEAYPAMLAAIGGARRSVALSSYIFRDDATGRA
FCAALKAAQDRGAEVRVILDGIGSGYFFPAAWRHLRRLGVPAGLFMHSVLPWRMPFLNLRTHKKLLIVDGRVAFTGGVNI
ADGNRVSEKPDFPIRDTHFRIEGPVVEHLTQAFIADWLFVSDEELEGEAWLPRLAPVGAVTARVVTSGPDADIEKIATVV
LQAIACAKESIRLTTPYFLPNELVLNALSLAAARGIAVDIAIPRKSDHRFVDWATHGHIDPLLKSGVRVWIDEPPFDHSK
AMTVDGQWCFIGSANWDSRSFRLNFELNVEVYDVALARALEAFIATKFQTPLRLADLDARSLPVRLRDAAVRLLLPYL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=458, Percent_Identity=29.0393013100437, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1787007, Length=339, Percent_Identity=29.4985250737463, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 52735; Mature: 52735

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEVLTRWLGTTASLRTEIVAAIGLVLTFGVSVHVLLRKRVVGAAIGWIGLAWLAPVFGT
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLYLTFGINRVERRARRLKRRPSQTIDETPQPSAHVPEAYHALDLAVQAVTGLPTVAGNR
HHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCH
FELLRNGDEAYPAMLAAIGGARRSVALSSYIFRDDATGRAFCAALKAAQDRGAEVRVILD
HHHHHCCCHHHHHHHHHHCCCHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCEEEEEEE
GIGSGYFFPAAWRHLRRLGVPAGLFMHSVLPWRMPFLNLRTHKKLLIVDGRVAFTGGVNI
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECEEEEECCEEE
ADGNRVSEKPDFPIRDTHFRIEGPVVEHLTQAFIADWLFVSDEELEGEAWLPRLAPVGAV
CCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHH
TARVVTSGPDADIEKIATVVLQAIACAKESIRLTTPYFLPNELVLNALSLAAARGIAVDI
HEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCEEEEE
AIPRKSDHRFVDWATHGHIDPLLKSGVRVWIDEPPFDHSKAMTVDGQWCFIGSANWDSRS
EECCCCCCCEEEEECCCCCCHHHHCCCEEEECCCCCCCCCEEEECCCEEEEECCCCCCCE
FRLNFELNVEVYDVALARALEAFIATKFQTPLRLADLDARSLPVRLRDAAVRLLLPYL
EEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MEEVLTRWLGTTASLRTEIVAAIGLVLTFGVSVHVLLRKRVVGAAIGWIGLAWLAPVFGT
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLYLTFGINRVERRARRLKRRPSQTIDETPQPSAHVPEAYHALDLAVQAVTGLPTVAGNR
HHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCH
FELLRNGDEAYPAMLAAIGGARRSVALSSYIFRDDATGRAFCAALKAAQDRGAEVRVILD
HHHHHCCCHHHHHHHHHHCCCHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCEEEEEEE
GIGSGYFFPAAWRHLRRLGVPAGLFMHSVLPWRMPFLNLRTHKKLLIVDGRVAFTGGVNI
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECEEEEECCEEE
ADGNRVSEKPDFPIRDTHFRIEGPVVEHLTQAFIADWLFVSDEELEGEAWLPRLAPVGAV
CCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHH
TARVVTSGPDADIEKIATVVLQAIACAKESIRLTTPYFLPNELVLNALSLAAARGIAVDI
HEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCEEEEE
AIPRKSDHRFVDWATHGHIDPLLKSGVRVWIDEPPFDHSKAMTVDGQWCFIGSANWDSRS
EECCCCCCCEEEEECCCCCCHHHHCCCEEEECCCCCCCCCEEEECCCEEEEECCCCCCCE
FRLNFELNVEVYDVALARALEAFIATKFQTPLRLADLDARSLPVRLRDAAVRLLLPYL
EEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]