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The map label for this gene is ywpJ [H]

Identifier: 218235937

GI number: 218235937

Start: 221522

End: 222394

Strand: Reverse

Name: ywpJ [H]

Synonym: BCB4264_A0250

Alternate gene names: 218235937

Gene position: 222394-221522 (Counterclockwise)

Preceding gene: 218231788

Following gene: 218231024

Centisome position: 4.1

GC content: 34.59

Gene sequence:

>873_bases
TTGAAATTAATCGCATTAGATATGGATGGTACACTACTATCATCTAATCTTGAAATCTCCAAAGAAAACCTACAAGCAAT
CCAAACTGCACAAGAAGCTGGTCATATTGTAATGATCTGTTCTGGTCGTGCGAAAGAGGATGCTTTAAAGCTATTGGAAG
AATATAAATTATCTCTTCCAGTCGGAGCAAGCAATGGGGCTATTGTTTATGTGGATGGAAAAGTAATTAACTCGCGTTGT
ATACAAAACGACAAAGTGTACAAACTTGCAAAATTACTAGAATCTGAAGGTTTCCCATATAAGCTATATACGAATAAAGG
AGTTTATTCTCCATATACATGGCAAGATCAAGTGATGCAAGCATTCGAAGAAAATAAGCATGCACTTGATGTTACATTAG
AGGAACTTGAACGAATTACAGAAAAGCAAAAGAAATCGAACCTAATTACTGATTTCAAAAAAATAGAAGATGTTGTAAAT
AACCAAGAACTAGAAATATCTAAATTCTTCATTTTAACGTTTAATGCAGCGCATCGCGCACAACTATTAAGTATATTACA
AGAAGATGCTGATATTATGGTTACAGCATCGGCTCCTACTAACTTAGAAATTATGGATAAGAATGGACATAAAGGAAACG
GGATACAGCAGATGGCAGCTCATTTTAATATCCCTATTGAAGATACAGTTGCTATTGGTGATAATTTTAATGATGTACCG
ATGTTAGAAGTAGCAGGCTTATCTGTTGCAATGGGCAACGCTGAAGAAGATGTAAAAAAGCTATGTGATGTTGTAACACT
AACAAACAATGAACATGGTGTTGCTCACGCAATCGAGCAATTTGTATTGAAACAAACTTCATCTAGTAAATAA

Upstream 100 bases:

>100_bases
TTACTTACTTTTAATATGAGATAAAATACCGCCTTTCCAAGATAAGTTTATATGTTTCCTCTATATAACTATGTATAATG
AAAACAAAGAGGTGGTCATT

Downstream 100 bases:

>100_bases
AAAAAGGACTCTTTCAATTTGAAAGAGTCCTTTTTTATACGTGATGACAAGCAACAAAATGCTCTGCACCAACATTACGG
AATTCTGGTACTATTTTTTT

Product: hydrolase, haloacid dehalogenase-like family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKLIALDMDGTLLSSNLEISKENLQAIQTAQEAGHIVMICSGRAKEDALKLLEEYKLSLPVGASNGAIVYVDGKVINSRC
IQNDKVYKLAKLLESEGFPYKLYTNKGVYSPYTWQDQVMQAFEENKHALDVTLEELERITEKQKKSNLITDFKKIEDVVN
NQELEISKFFILTFNAAHRAQLLSILQEDADIMVTASAPTNLEIMDKNGHKGNGIQQMAAHFNIPIEDTVAIGDNFNDVP
MLEVAGLSVAMGNAEEDVKKLCDVVTLTNNEHGVAHAIEQFVLKQTSSSK

Sequences:

>Translated_290_residues
MKLIALDMDGTLLSSNLEISKENLQAIQTAQEAGHIVMICSGRAKEDALKLLEEYKLSLPVGASNGAIVYVDGKVINSRC
IQNDKVYKLAKLLESEGFPYKLYTNKGVYSPYTWQDQVMQAFEENKHALDVTLEELERITEKQKKSNLITDFKKIEDVVN
NQELEISKFFILTFNAAHRAQLLSILQEDADIMVTASAPTNLEIMDKNGHKGNGIQQMAAHFNIPIEDTVAIGDNFNDVP
MLEVAGLSVAMGNAEEDVKKLCDVVTLTNNEHGVAHAIEQFVLKQTSSSK
>Mature_290_residues
MKLIALDMDGTLLSSNLEISKENLQAIQTAQEAGHIVMICSGRAKEDALKLLEEYKLSLPVGASNGAIVYVDGKVINSRC
IQNDKVYKLAKLLESEGFPYKLYTNKGVYSPYTWQDQVMQAFEENKHALDVTLEELERITEKQKKSNLITDFKKIEDVVN
NQELEISKFFILTFNAAHRAQLLSILQEDADIMVTASAPTNLEIMDKNGHKGNGIQQMAAHFNIPIEDTVAIGDNFNDVP
MLEVAGLSVAMGNAEEDVKKLCDVVTLTNNEHGVAHAIEQFVLKQTSSSK

Specific function: Putative phosphatase (Potential) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI2367265, Length=294, Percent_Identity=31.9727891156463, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787043, Length=290, Percent_Identity=26.2068965517241, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI87081790, Length=293, Percent_Identity=26.2798634812287, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 32175; Mature: 32175

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIALDMDGTLLSSNLEISKENLQAIQTAQEAGHIVMICSGRAKEDALKLLEEYKLSLP
CEEEEEECCCEEEECCCEECHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCC
VGASNGAIVYVDGKVINSRCIQNDKVYKLAKLLESEGFPYKLYTNKGVYSPYTWQDQVMQ
CCCCCCEEEEECCEEECCHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHH
AFEENKHALDVTLEELERITEKQKKSNLITDFKKIEDVVNNQELEISKFFILTFNAAHRA
HHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHH
QLLSILQEDADIMVTASAPTNLEIMDKNGHKGNGIQQMAAHFNIPIEDTVAIGDNFNDVP
HHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCC
MLEVAGLSVAMGNAEEDVKKLCDVVTLTNNEHGVAHAIEQFVLKQTSSSK
EEEECCCEEEECCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLIALDMDGTLLSSNLEISKENLQAIQTAQEAGHIVMICSGRAKEDALKLLEEYKLSLP
CEEEEEECCCEEEECCCEECHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCC
VGASNGAIVYVDGKVINSRCIQNDKVYKLAKLLESEGFPYKLYTNKGVYSPYTWQDQVMQ
CCCCCCEEEEECCEEECCHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHH
AFEENKHALDVTLEELERITEKQKKSNLITDFKKIEDVVNNQELEISKFFILTFNAAHRA
HHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHH
QLLSILQEDADIMVTASAPTNLEIMDKNGHKGNGIQQMAAHFNIPIEDTVAIGDNFNDVP
HHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCC
MLEVAGLSVAMGNAEEDVKKLCDVVTLTNNEHGVAHAIEQFVLKQTSSSK
EEEECCCEEEECCCHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]