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The map label for this gene is kbl [C]

Identifier: 218235388

GI number: 218235388

Start: 644646

End: 645836

Strand: Direct

Name: kbl [C]

Synonym: BCB4264_A0657

Alternate gene names: 218235388

Gene position: 644646-645836 (Clockwise)

Preceding gene: 218235984

Following gene: 218236132

Centisome position: 11.9

GC content: 36.1

Gene sequence:

>1191_bases
ATGTCTAGTAAAACACTTGCGAAATTTTTAGAAGAAAATTTAGAGGATTTAAAATCAAAGGGGCTTTATAACGTAATTGA
TCCGCTTGAGAGTTCAAATGGACCAATCATTACAATTGGCGGAAAAGAATATATTAACTTATCTTCAAACAACTATCTTG
GATTAGCAACAGATAGCCGTTTGCAAGAAGCAGCAATTGGTGCTATTCATAAGTACGGTGTTGGAGCAGGGGCTGTTCGT
ACAATTAACGGTACTTTAGATTTGCATATTAAGTTAGAAGAAACAATTGCAAAGTTTAAACATACAGAAGCAGCAATTGC
TTATCAATCAGGATTTAACTGTAATATGGCAGCGATTTCAGCTGTTATGGATAAAAATGATGCAATTCTTTCAGACGAAT
TAAACCATGCTTCTATTATTGATGGTAGTCGTCTATCAAAAGCAAAAATTATTGTTTATAAACATTCGGATATGGAAGAT
TTACGCCAAAAAGCAATTGCGGCGAAAGAATCAGGTCTTTACAATAAATTAATGGTAATTACAGACGGTGTCTTTTCAAT
GGATGGAGATGTTGCAAAACTACCAGAAATTGTTGAGATTGCAGAAGAGTTAGATTTAATGACATACGTAGATGATGCAC
ACGGTTCAGGTGTACTTGGAAAAGGTGCAGGAACTGTAAAGCACTTCGGTCTTTCTGATAAAGTTGATTTCCAAATTGGT
ACATTATCAAAAGCAATTGGGGTAATTGGTGGATATGTAGCAGGGAAACAAAACTTAATTGACTGGTTAAAAGTTCGTTC
ACGTCCATTCTTATTCTCTACAGCATTAACACCAGCTGATGCAGCAGCTTGCATGAGATCAATTGAAATCTTAATGGAAA
GCACAGAGTTACACGATCGCTTATGGGAAAATGGCCGCTACTTGAAACAAGGATTAAAAGAACTTGGCTTTAACATTGGA
GAAAGTGAAACGCCAATTACACCTTGTATCATTGGGGACGAAGTATTAACACAAGAATTTAGTAAACGTCTAAATGAAGA
AGGGGTATACGCAAAATCTATCGTGTTCCCAACTGTAGCAAAAGGAACAGGGCGCGTTCGTAATATGCCTACAGCAGCTC
ATACGAAAGAAATGTTAGATGAAGCAATTCTTAAGTATGAAAAAGTAGGAAAAGAAATGGGCATCATTTAA

Upstream 100 bases:

>100_bases
TTTTTCTATAATAAATTCTGAAAACGTGTTAAGCTAGTAGAGGGATTATTAGAACTATTAAGACAATAATCCGCTTACAT
GAACGATGGGAGGCTTCACG

Downstream 100 bases:

>100_bases
GTAACGACATCTTTTGAATAGCTTGCAAATGAGGAGTGGGGAAAATGAAAAAAATTCTAGTAACCGGTTCTTTAGGGCAA
ATTGGTTCTGAACTAGTAAT

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase 1; 7-keto-8-amino-pelargonic acid synthase 1; 7-KAP synthase 1; KAPA synthase 1; 8-amino-7-ketopelargonate synthase 1; Alpha-oxoamine synthase 1; L-alanine--pimeloyl-CoA ligase 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSSKTLAKFLEENLEDLKSKGLYNVIDPLESSNGPIITIGGKEYINLSSNNYLGLATDSRLQEAAIGAIHKYGVGAGAVR
TINGTLDLHIKLEETIAKFKHTEAAIAYQSGFNCNMAAISAVMDKNDAILSDELNHASIIDGSRLSKAKIIVYKHSDMED
LRQKAIAAKESGLYNKLMVITDGVFSMDGDVAKLPEIVEIAEELDLMTYVDDAHGSGVLGKGAGTVKHFGLSDKVDFQIG
TLSKAIGVIGGYVAGKQNLIDWLKVRSRPFLFSTALTPADAAACMRSIEILMESTELHDRLWENGRYLKQGLKELGFNIG
ESETPITPCIIGDEVLTQEFSKRLNEEGVYAKSIVFPTVAKGTGRVRNMPTAAHTKEMLDEAILKYEKVGKEMGII

Sequences:

>Translated_396_residues
MSSKTLAKFLEENLEDLKSKGLYNVIDPLESSNGPIITIGGKEYINLSSNNYLGLATDSRLQEAAIGAIHKYGVGAGAVR
TINGTLDLHIKLEETIAKFKHTEAAIAYQSGFNCNMAAISAVMDKNDAILSDELNHASIIDGSRLSKAKIIVYKHSDMED
LRQKAIAAKESGLYNKLMVITDGVFSMDGDVAKLPEIVEIAEELDLMTYVDDAHGSGVLGKGAGTVKHFGLSDKVDFQIG
TLSKAIGVIGGYVAGKQNLIDWLKVRSRPFLFSTALTPADAAACMRSIEILMESTELHDRLWENGRYLKQGLKELGFNIG
ESETPITPCIIGDEVLTQEFSKRLNEEGVYAKSIVFPTVAKGTGRVRNMPTAAHTKEMLDEAILKYEKVGKEMGII
>Mature_395_residues
SSKTLAKFLEENLEDLKSKGLYNVIDPLESSNGPIITIGGKEYINLSSNNYLGLATDSRLQEAAIGAIHKYGVGAGAVRT
INGTLDLHIKLEETIAKFKHTEAAIAYQSGFNCNMAAISAVMDKNDAILSDELNHASIIDGSRLSKAKIIVYKHSDMEDL
RQKAIAAKESGLYNKLMVITDGVFSMDGDVAKLPEIVEIAEELDLMTYVDDAHGSGVLGKGAGTVKHFGLSDKVDFQIGT
LSKAIGVIGGYVAGKQNLIDWLKVRSRPFLFSTALTPADAAACMRSIEILMESTELHDRLWENGRYLKQGLKELGFNIGE
SETPITPCIIGDEVLTQEFSKRLNEEGVYAKSIVFPTVAKGTGRVRNMPTAAHTKEMLDEAILKYEKVGKEMGII

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=395, Percent_Identity=35.4430379746835, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI284448556, Length=354, Percent_Identity=36.4406779661017, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI4758668, Length=421, Percent_Identity=33.729216152019, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI119220554, Length=419, Percent_Identity=31.0262529832936, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI83977442, Length=355, Percent_Identity=29.2957746478873, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI83977444, Length=355, Percent_Identity=29.2957746478873, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI83977440, Length=355, Percent_Identity=29.2957746478873, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI4502025, Length=405, Percent_Identity=30.1234567901235, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI40316939, Length=405, Percent_Identity=30.1234567901235, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI5454084, Length=314, Percent_Identity=29.9363057324841, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1790046, Length=390, Percent_Identity=38.974358974359, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1786993, Length=357, Percent_Identity=31.9327731092437, Blast_Score=178, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI71994529, Length=393, Percent_Identity=37.9134860050891, Blast_Score=260, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI32566772, Length=368, Percent_Identity=32.6086956521739, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17560914, Length=355, Percent_Identity=32.9577464788732, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17560912, Length=355, Percent_Identity=32.9577464788732, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI71982617, Length=304, Percent_Identity=28.6184210526316, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI71982625, Length=306, Percent_Identity=29.4117647058824, Blast_Score=140, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6320267, Length=390, Percent_Identity=31.2820512820513, Blast_Score=193, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6320438, Length=373, Percent_Identity=32.171581769437, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6323954, Length=259, Percent_Identity=28.1853281853282, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24662918, Length=395, Percent_Identity=37.7215189873418, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI17137420, Length=348, Percent_Identity=34.4827586206897, Blast_Score=188, Evalue=7e-48,
Organism=Drosophila melanogaster, GI17136286, Length=368, Percent_Identity=30.4347826086957, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24653280, Length=291, Percent_Identity=28.5223367697594, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24653276, Length=291, Percent_Identity=28.5223367697594, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24653278, Length=291, Percent_Identity=28.5223367697594, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR010962
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 43064; Mature: 42933

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKTLAKFLEENLEDLKSKGLYNVIDPLESSNGPIITIGGKEYINLSSNNYLGLATDSR
CCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEECCCEEEEECCCCEEEECCCHH
LQEAAIGAIHKYGVGAGAVRTINGTLDLHIKLEETIAKFKHTEAAIAYQSGFNCNMAAIS
HHHHHHHHHHHHCCCCCCEEEECCEEEEEEEEHHHHHHHHHHHHHEEECCCCCCCHHHHH
AVMDKNDAILSDELNHASIIDGSRLSKAKIIVYKHSDMEDLRQKAIAAKESGLYNKLMVI
HHHCCCCCEECCCCCCCEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHEEEEE
TDGVFSMDGDVAKLPEIVEIAEELDLMTYVDDAHGSGVLGKGAGTVKHFGLSDKVDFQIG
ECCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCEECCCCCCCCEEHH
TLSKAIGVIGGYVAGKQNLIDWLKVRSRPFLFSTALTPADAAACMRSIEILMESTELHDR
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LWENGRYLKQGLKELGFNIGESETPITPCIIGDEVLTQEFSKRLNEEGVYAKSIVFPTVA
HHHCCHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCEEHHEECCHHC
KGTGRVRNMPTAAHTKEMLDEAILKYEKVGKEMGII
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSKTLAKFLEENLEDLKSKGLYNVIDPLESSNGPIITIGGKEYINLSSNNYLGLATDSR
CHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEECCCEEEEECCCCEEEECCCHH
LQEAAIGAIHKYGVGAGAVRTINGTLDLHIKLEETIAKFKHTEAAIAYQSGFNCNMAAIS
HHHHHHHHHHHHCCCCCCEEEECCEEEEEEEEHHHHHHHHHHHHHEEECCCCCCCHHHHH
AVMDKNDAILSDELNHASIIDGSRLSKAKIIVYKHSDMEDLRQKAIAAKESGLYNKLMVI
HHHCCCCCEECCCCCCCEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHEEEEE
TDGVFSMDGDVAKLPEIVEIAEELDLMTYVDDAHGSGVLGKGAGTVKHFGLSDKVDFQIG
ECCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCEECCCCCCCCEEHH
TLSKAIGVIGGYVAGKQNLIDWLKVRSRPFLFSTALTPADAAACMRSIEILMESTELHDR
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LWENGRYLKQGLKELGFNIGESETPITPCIIGDEVLTQEFSKRLNEEGVYAKSIVFPTVA
HHHCCHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCEEHHEECCHHC
KGTGRVRNMPTAAHTKEMLDEAILKYEKVGKEMGII
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]