Click here to switch to the map view.

The map label for this gene is ykfB [H]

Identifier: 218235072

GI number: 218235072

Start: 361418

End: 362527

Strand: Reverse

Name: ykfB [H]

Synonym: BCB4264_A0386

Alternate gene names: 218235072

Gene position: 362527-361418 (Counterclockwise)

Preceding gene: 218233134

Following gene: 218231711

Centisome position: 6.69

GC content: 38.92

Gene sequence:

>1110_bases
ATGAAAATTACAGCTATTCATCTTTACGCAATTCGTTTACCGCTTCGCAATCCGTTTGTTATTAGTTATGGCTCTTATTC
TGATATGCCCTCTATTATCGTCAAAATGGAAACGGATGAAGGTATTATCGGTTACGGTGAAGGCGTTGCTGATGATCACG
TCACAGGTGAATCATGGGAAAGTACTTTCCATACTTTAAAACATACGCTAACCCCTGCTCTAATCGGGCAAAATCCAATG
AATATCGAAAAAATACACGACATGATGGACAATACAATTTACGGTGTTCCAACAGCGAAAGCCGCAATTGATATTGCTTG
TTTTGATATAATGGGCAAAAAACTAAATCAACCTGTATATCAATTAATTGGCGGGCGCTACCATGAAGAATTCCCTGTCA
CTCACGTTTTAAGTATTGCCGATCCAGAAAACATGGCTGAAGAAGCTGCTTCTATGATTCAAAAAGGTTACCAATCTTTC
AAAATGAAAGTTGGTACAAATGTAAAAGAAGATGTAAAACGTATTGAAGCTGTACGAGAACGTGTAGGAAATGACATCGC
TATTCGCGTTGATGTAAACCAAGGCTGGAAAAATAGCGCAAACACATTAACAGCACTACGTTCATTAGGACATTTAAACA
TTGACTGGATTGAACAACCTGTTATCGCGGACGATATTGATGCCATGGCTCATATTCGTTCTAAAACAGACCTCCCGCTT
ATGATTGATGAAGGATTAAAAAGTTCTCGTGAAATGCGCCAAATTATTAAATTAGAAGCTGCTGATAAAGTAAATATAAA
ACTAATGAAATGCGGCGGCATATATCCAGCTGTAAAGCTCGCTCATCAAGCTGAAATGGCCGGCATTGAATGCCAAGTAG
GATCAATGGTCGAATCATCTGTTGCCTCTTCCGCAGGATTCCATGTCGCTTTCTCAAAGAAAATCATTACGAGCGTAGAG
CTAACAGGACCATTAAAATTCACAAAAGACATTGGAAACTTACATTACGATGTACCATTCATTCGCTTAAACGAAAAGCC
GGGACTCGGCATTGAAATAAACGAAGATACATTACAAGAACTAACCGTCTTTCAAGACGTTGTACGCTAA

Upstream 100 bases:

>100_bases
TCATCATTTTCTCTAACTCCTTATCATTAATTAACGACTATTTAACGTCAACTATATCAAATGTATAAAACTATTGTAAA
CAGGAAGGGGTTTCTATTCA

Downstream 100 bases:

>100_bases
AGGAGGACTATCATATGACGGTTCTATACGAAGGAACATTAAAACAAAATAACGAATCATTTCACGTTGCATTACTATCA
CTAGAGCACATCGAACAAAT

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKITAIHLYAIRLPLRNPFVISYGSYSDMPSIIVKMETDEGIIGYGEGVADDHVTGESWESTFHTLKHTLTPALIGQNPM
NIEKIHDMMDNTIYGVPTAKAAIDIACFDIMGKKLNQPVYQLIGGRYHEEFPVTHVLSIADPENMAEEAASMIQKGYQSF
KMKVGTNVKEDVKRIEAVRERVGNDIAIRVDVNQGWKNSANTLTALRSLGHLNIDWIEQPVIADDIDAMAHIRSKTDLPL
MIDEGLKSSREMRQIIKLEAADKVNIKLMKCGGIYPAVKLAHQAEMAGIECQVGSMVESSVASSAGFHVAFSKKIITSVE
LTGPLKFTKDIGNLHYDVPFIRLNEKPGLGIEINEDTLQELTVFQDVVR

Sequences:

>Translated_369_residues
MKITAIHLYAIRLPLRNPFVISYGSYSDMPSIIVKMETDEGIIGYGEGVADDHVTGESWESTFHTLKHTLTPALIGQNPM
NIEKIHDMMDNTIYGVPTAKAAIDIACFDIMGKKLNQPVYQLIGGRYHEEFPVTHVLSIADPENMAEEAASMIQKGYQSF
KMKVGTNVKEDVKRIEAVRERVGNDIAIRVDVNQGWKNSANTLTALRSLGHLNIDWIEQPVIADDIDAMAHIRSKTDLPL
MIDEGLKSSREMRQIIKLEAADKVNIKLMKCGGIYPAVKLAHQAEMAGIECQVGSMVESSVASSAGFHVAFSKKIITSVE
LTGPLKFTKDIGNLHYDVPFIRLNEKPGLGIEINEDTLQELTVFQDVVR
>Mature_369_residues
MKITAIHLYAIRLPLRNPFVISYGSYSDMPSIIVKMETDEGIIGYGEGVADDHVTGESWESTFHTLKHTLTPALIGQNPM
NIEKIHDMMDNTIYGVPTAKAAIDIACFDIMGKKLNQPVYQLIGGRYHEEFPVTHVLSIADPENMAEEAASMIQKGYQSF
KMKVGTNVKEDVKRIEAVRERVGNDIAIRVDVNQGWKNSANTLTALRSLGHLNIDWIEQPVIADDIDAMAHIRSKTDLPL
MIDEGLKSSREMRQIIKLEAADKVNIKLMKCGGIYPAVKLAHQAEMAGIECQVGSMVESSVASSAGFHVAFSKKIITSVE
LTGPLKFTKDIGNLHYDVPFIRLNEKPGLGIEINEDTLQELTVFQDVVR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=389, Percent_Identity=26.7352185089974, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081876, Length=330, Percent_Identity=28.7878787878788, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI226510960, Length=352, Percent_Identity=23.5795454545455, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 40936; Mature: 40936

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITAIHLYAIRLPLRNPFVISYGSYSDMPSIIVKMETDEGIIGYGEGVADDHVTGESWE
CEEEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCHHH
STFHTLKHTLTPALIGQNPMNIEKIHDMMDNTIYGVPTAKAAIDIACFDIMGKKLNQPVY
HHHHHHHHHHCHHHHCCCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCHHH
QLIGGRYHEEFPVTHVLSIADPENMAEEAASMIQKGYQSFKMKVGTNVKEDVKRIEAVRE
HHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RVGNDIAIRVDVNQGWKNSANTLTALRSLGHLNIDWIEQPVIADDIDAMAHIRSKTDLPL
HCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCE
MIDEGLKSSREMRQIIKLEAADKVNIKLMKCGGIYPAVKLAHQAEMAGIECQVGSMVESS
EECHHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEHHHHHHHH
VASSAGFHVAFSKKIITSVELTGPLKFTKDIGNLHYDVPFIRLNEKPGLGIEINEDTLQE
HHHCCCEEEEEHHHHHEEEEECCCCHHHHHHCCEEECCCEEEECCCCCCCEEECHHHHHH
LTVFQDVVR
HHHHHHHHC
>Mature Secondary Structure
MKITAIHLYAIRLPLRNPFVISYGSYSDMPSIIVKMETDEGIIGYGEGVADDHVTGESWE
CEEEEEEEEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCHHH
STFHTLKHTLTPALIGQNPMNIEKIHDMMDNTIYGVPTAKAAIDIACFDIMGKKLNQPVY
HHHHHHHHHHCHHHHCCCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHCCHHH
QLIGGRYHEEFPVTHVLSIADPENMAEEAASMIQKGYQSFKMKVGTNVKEDVKRIEAVRE
HHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RVGNDIAIRVDVNQGWKNSANTLTALRSLGHLNIDWIEQPVIADDIDAMAHIRSKTDLPL
HCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCE
MIDEGLKSSREMRQIIKLEAADKVNIKLMKCGGIYPAVKLAHQAEMAGIECQVGSMVESS
EECHHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEHHHHHHHH
VASSAGFHVAFSKKIITSVELTGPLKFTKDIGNLHYDVPFIRLNEKPGLGIEINEDTLQE
HHHCCCEEEEEHHHHHEEEEECCCCHHHHHHCCEEECCCEEEECCCCCCCEEECHHHHHH
LTVFQDVVR
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]