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The map label for this gene is brnQ2 [H]

Identifier: 218233137

GI number: 218233137

Start: 828175

End: 829491

Strand: Reverse

Name: brnQ2 [H]

Synonym: BCB4264_A0856

Alternate gene names: 218233137

Gene position: 829491-828175 (Counterclockwise)

Preceding gene: 218232360

Following gene: 218235353

Centisome position: 15.31

GC content: 35.84

Gene sequence:

>1317_bases
ATGCGTACAACTTTGAAACCAGCGCAAATACTTTCGATTAGTTTATTACTATTCGCAGTTTTCTTCGGTGCTGGTAATAT
GATTTTCCCGCCCCTTCTTGGTCTTTCTTCCGGAGAAAATATGTGGGTTTCCATTACAGGGTTTATTATTACAGACGTCG
GTTTATCTTTACTTGCTATCGTTGCTGTTGCCCTCGCTGGTGGTAGTTTTAACACTTTAGCGAGCCGTGTTCATCCAAAA
TTCGCAGCAGTATTCGCTATCATTATTTATCTTTCAATCGGCCCACTATTTGTTATTCCACGAACTGGATCTGTATCTTA
TGAAATTGGGATTGCACCACTTTTCCCAGATCAATGGTATTCTATGTTAGTTTTTAGTGCGATTTTCTTTACAGTCGTTT
ACTTCTTATCATTAAATCCATCCAAGCTAGTAGATCATATCGGTAAAATATTAACGCCAATTTTACTTGTAATTATTGCA
ATTATGGCAACAAAAGCGATTCTTTCACCAGGAACGTTTGCAGAGCCTATCGGTGACTATAAAGACATCCCGTTTTTCAA
AGGATTTCTTGAAGGATTTTTAACGTTAGATGCAATTGGCGCTCTCGTACTATCAACAATTGTTGTAAATGCAATTCGTC
AAAACGGTATACAAGAGAAGAAATCTATCGCGAAATATACAATCATTTGCGGAAGTATCGCTGCTCTATTCTTAACAATT
GTTTACTTCTTACTCGGTTATATCGGAGCTTCAAACGGAAACTTGGGACAATTCGAGAACGGTGGTCAACTATTAGCAAC
TGTTATGTACCAATTCTTTGGGACAAGCGGTAATGTTTTATTAAGTATCGCCATTATCTTCGCTTGTTTAACAACAGCAA
TTGGTGTTGTTAGTGCATTTGCAAACTATTTCGCAACAGTATTAACAAATGTTTCTTACAAAAAGCTCGTACTATACGTT
TGTATCTTTAGTTTCGTTATTTCAAACTTAGGATTAAGCTTATTAATTAAAATCACACTACCTGTATTAATTATTTTATA
TCCAATTACGATTATTTTAATTTTCGTATCATTTATCGATAAATATACGAAACGTAAACCTTCTGTCTACATCGGAGCAA
TGATCGCAGCATTCATTATTAGCTGTATTCATGCACTTGATAATGTGGGAATGATACCAAGTTTTATCGCTAATATCGTA
CACACAATTCCTTTTTATAACTTAGGAATCGGCTGGATCGTTCCAGCAATAATCGGTGGTATAATCGGATACTTTATTCC
GCAAACAGAAGCTGAAGGTGAAGTTTCTGCAAAGTAA

Upstream 100 bases:

>100_bases
CCTATTGCTTTCTAGTCATCAGACCTTTAAAATAATAACAGTTACTCGACAAATTATTTGAATTTTCGACAAAAATACTC
ATTAGGAGGATTTTCTTTAT

Downstream 100 bases:

>100_bases
AAAGAAGCAAGCATGAAATCCATGCTTGCTTCTTTTTCTCTTCACATCGTATGTTTAAACGTAAACAACTCTTCCTTTGA
CTGTACAGCAGATTGTTCCT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MRTTLKPAQILSISLLLFAVFFGAGNMIFPPLLGLSSGENMWVSITGFIITDVGLSLLAIVAVALAGGSFNTLASRVHPK
FAAVFAIIIYLSIGPLFVIPRTGSVSYEIGIAPLFPDQWYSMLVFSAIFFTVVYFLSLNPSKLVDHIGKILTPILLVIIA
IMATKAILSPGTFAEPIGDYKDIPFFKGFLEGFLTLDAIGALVLSTIVVNAIRQNGIQEKKSIAKYTIICGSIAALFLTI
VYFLLGYIGASNGNLGQFENGGQLLATVMYQFFGTSGNVLLSIAIIFACLTTAIGVVSAFANYFATVLTNVSYKKLVLYV
CIFSFVISNLGLSLLIKITLPVLIILYPITIILIFVSFIDKYTKRKPSVYIGAMIAAFIISCIHALDNVGMIPSFIANIV
HTIPFYNLGIGWIVPAIIGGIIGYFIPQTEAEGEVSAK

Sequences:

>Translated_438_residues
MRTTLKPAQILSISLLLFAVFFGAGNMIFPPLLGLSSGENMWVSITGFIITDVGLSLLAIVAVALAGGSFNTLASRVHPK
FAAVFAIIIYLSIGPLFVIPRTGSVSYEIGIAPLFPDQWYSMLVFSAIFFTVVYFLSLNPSKLVDHIGKILTPILLVIIA
IMATKAILSPGTFAEPIGDYKDIPFFKGFLEGFLTLDAIGALVLSTIVVNAIRQNGIQEKKSIAKYTIICGSIAALFLTI
VYFLLGYIGASNGNLGQFENGGQLLATVMYQFFGTSGNVLLSIAIIFACLTTAIGVVSAFANYFATVLTNVSYKKLVLYV
CIFSFVISNLGLSLLIKITLPVLIILYPITIILIFVSFIDKYTKRKPSVYIGAMIAAFIISCIHALDNVGMIPSFIANIV
HTIPFYNLGIGWIVPAIIGGIIGYFIPQTEAEGEVSAK
>Mature_438_residues
MRTTLKPAQILSISLLLFAVFFGAGNMIFPPLLGLSSGENMWVSITGFIITDVGLSLLAIVAVALAGGSFNTLASRVHPK
FAAVFAIIIYLSIGPLFVIPRTGSVSYEIGIAPLFPDQWYSMLVFSAIFFTVVYFLSLNPSKLVDHIGKILTPILLVIIA
IMATKAILSPGTFAEPIGDYKDIPFFKGFLEGFLTLDAIGALVLSTIVVNAIRQNGIQEKKSIAKYTIICGSIAALFLTI
VYFLLGYIGASNGNLGQFENGGQLLATVMYQFFGTSGNVLLSIAIIFACLTTAIGVVSAFANYFATVLTNVSYKKLVLYV
CIFSFVISNLGLSLLIKITLPVLIILYPITIILIFVSFIDKYTKRKPSVYIGAMIAAFIISCIHALDNVGMIPSFIANIV
HTIPFYNLGIGWIVPAIIGGIIGYFIPQTEAEGEVSAK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=423, Percent_Identity=35.9338061465721, Blast_Score=274, Evalue=9e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47156; Mature: 47156

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTLKPAQILSISLLLFAVFFGAGNMIFPPLLGLSSGENMWVSITGFIITDVGLSLLAI
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
VAVALAGGSFNTLASRVHPKFAAVFAIIIYLSIGPLFVIPRTGSVSYEIGIAPLFPDQWY
HHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCHHHHH
SMLVFSAIFFTVVYFLSLNPSKLVDHIGKILTPILLVIIAIMATKAILSPGTFAEPIGDY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
KDIPFFKGFLEGFLTLDAIGALVLSTIVVNAIRQNGIQEKKSIAKYTIICGSIAALFLTI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VYFLLGYIGASNGNLGQFENGGQLLATVMYQFFGTSGNVLLSIAIIFACLTTAIGVVSAF
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ANYFATVLTNVSYKKLVLYVCIFSFVISNLGLSLLIKITLPVLIILYPITIILIFVSFID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYTKRKPSVYIGAMIAAFIISCIHALDNVGMIPSFIANIVHTIPFYNLGIGWIVPAIIGG
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHCCHHHHHHHHHHH
IIGYFIPQTEAEGEVSAK
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRTTLKPAQILSISLLLFAVFFGAGNMIFPPLLGLSSGENMWVSITGFIITDVGLSLLAI
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
VAVALAGGSFNTLASRVHPKFAAVFAIIIYLSIGPLFVIPRTGSVSYEIGIAPLFPDQWY
HHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCHHHHH
SMLVFSAIFFTVVYFLSLNPSKLVDHIGKILTPILLVIIAIMATKAILSPGTFAEPIGDY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
KDIPFFKGFLEGFLTLDAIGALVLSTIVVNAIRQNGIQEKKSIAKYTIICGSIAALFLTI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VYFLLGYIGASNGNLGQFENGGQLLATVMYQFFGTSGNVLLSIAIIFACLTTAIGVVSAF
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ANYFATVLTNVSYKKLVLYVCIFSFVISNLGLSLLIKITLPVLIILYPITIILIFVSFID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYTKRKPSVYIGAMIAAFIISCIHALDNVGMIPSFIANIVHTIPFYNLGIGWIVPAIIGG
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHCCHHHHHHHHHHH
IIGYFIPQTEAEGEVSAK
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]