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The map label for this gene is glnH [H]

Identifier: 218232444

GI number: 218232444

Start: 667775

End: 668605

Strand: Direct

Name: glnH [H]

Synonym: BCB4264_A0676

Alternate gene names: 218232444

Gene position: 667775-668605 (Clockwise)

Preceding gene: 218233064

Following gene: 218234515

Centisome position: 12.32

GC content: 37.06

Gene sequence:

>831_bases
ATGCTTAAAATGAAAAAGTTGTTCACCTTAATTGTATTTTCATGTTTATTCGTACTTGTCGTTGCTGGGTGCGGAAGTAA
AAAAGATGAGGCGAAAGAAACGAATACGAAGCAAGGCGGGGCCGTTGAGCAAATTAAGAAGCGCGGGAAACTAGTAGTTG
GGGTGAAGAATGATACGAACTTATTTGGATTGAAAAACCCTTCCACAGGGCAGGTAGAAGGATTTGATGTTGATGTTGCA
AAGGCGCTTGCGAAAAAAATCCTCGGAGATGAAAAGAAACTAGAATTAAAAGAAGTAACGTCTAAAACGCGTATTCCACT
CCTGAAAAACGGTGACATTGATGCAATTATCGCAACAATGACAATTACGGAAGAACGTAAAAAAGAGGTTGATTTTTCAG
ATGTATATTTTAAAGCAGGGCAGTCGTTACTCGTGAAAAAAGGGAGCAATATTAAAAGTATTGATGATGTGAAAAAGGGT
GTGAAAGTGTTAGCCGTAAAAGGTTCAACATCGACAAATAACATTCGCCAAAAATCACCGGAAGCGACTGTTTTAGAATT
TGAAAATTACAGTGAGGCATTTACGGCGTTAAAAGCAGGAAAAGGTGATGTTTTAACGACAGATAATGCAATTCTTTACG
GCATGGCAAAACAAGATTCAAATTATGAAGTTGTAGGGAAAATTTTTACGGATGAACCATACGGAATTGCAGTGCAAAAA
GGTGCAGATGATTTGACGAAAGAGATTAATAGCTTACTAAAAGATATGAAAGCGAATGGAGAATACGATAAATTGTATGA
GAAATGGATTGGTCAAAAACAAGAGAAGTAA

Upstream 100 bases:

>100_bases
CATTGAGGATACAACACCGGCGCAATTTTTTGCGAGTCCTGAACAAGAAAGAGCACGTTTATTTTTAAGTCGAGTGTTAA
ATCATTAAAGGAGGAATTTT

Downstream 100 bases:

>100_bases
AGTGAAACTCTAATTAGTGGGGCTTTATTCTTATTGAAGAGTAAGAGGATGAGGGATGCTCATCCTCTTCTTGTAAAGAA
AGAGGGGAGAATATGTGCCT

Product: amino acid ABC transporter, amino acid-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MLKMKKLFTLIVFSCLFVLVVAGCGSKKDEAKETNTKQGGAVEQIKKRGKLVVGVKNDTNLFGLKNPSTGQVEGFDVDVA
KALAKKILGDEKKLELKEVTSKTRIPLLKNGDIDAIIATMTITEERKKEVDFSDVYFKAGQSLLVKKGSNIKSIDDVKKG
VKVLAVKGSTSTNNIRQKSPEATVLEFENYSEAFTALKAGKGDVLTTDNAILYGMAKQDSNYEVVGKIFTDEPYGIAVQK
GADDLTKEINSLLKDMKANGEYDKLYEKWIGQKQEK

Sequences:

>Translated_276_residues
MLKMKKLFTLIVFSCLFVLVVAGCGSKKDEAKETNTKQGGAVEQIKKRGKLVVGVKNDTNLFGLKNPSTGQVEGFDVDVA
KALAKKILGDEKKLELKEVTSKTRIPLLKNGDIDAIIATMTITEERKKEVDFSDVYFKAGQSLLVKKGSNIKSIDDVKKG
VKVLAVKGSTSTNNIRQKSPEATVLEFENYSEAFTALKAGKGDVLTTDNAILYGMAKQDSNYEVVGKIFTDEPYGIAVQK
GADDLTKEINSLLKDMKANGEYDKLYEKWIGQKQEK
>Mature_276_residues
MLKMKKLFTLIVFSCLFVLVVAGCGSKKDEAKETNTKQGGAVEQIKKRGKLVVGVKNDTNLFGLKNPSTGQVEGFDVDVA
KALAKKILGDEKKLELKEVTSKTRIPLLKNGDIDAIIATMTITEERKKEVDFSDVYFKAGQSLLVKKGSNIKSIDDVKKG
VKVLAVKGSTSTNNIRQKSPEATVLEFENYSEAFTALKAGKGDVLTTDNAILYGMAKQDSNYEVVGKIFTDEPYGIAVQK
GADDLTKEINSLLKDMKANGEYDKLYEKWIGQKQEK

Specific function: Part of the ABC transporter complex glnHMPQ involved in glutamine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=239, Percent_Identity=34.7280334728033, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788228, Length=273, Percent_Identity=32.2344322344322, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1786876, Length=243, Percent_Identity=29.2181069958848, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787088, Length=208, Percent_Identity=32.6923076923077, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787085, Length=210, Percent_Identity=30.4761904761905, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=29.8245614035088, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788649, Length=211, Percent_Identity=29.8578199052133, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30331; Mature: 30331

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKMKKLFTLIVFSCLFVLVVAGCGSKKDEAKETNTKQGGAVEQIKKRGKLVVGVKNDTN
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHCCCEEEEECCCCC
LFGLKNPSTGQVEGFDVDVAKALAKKILGDEKKLELKEVTSKTRIPLLKNGDIDAIIATM
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCEEEEEE
TITEERKKEVDFSDVYFKAGQSLLVKKGSNIKSIDDVKKGVKVLAVKGSTSTNNIRQKSP
EECHHHHHCCCHHHHHHHCCCEEEEECCCCCCHHHHHHCCEEEEEEECCCCCCCHHHCCC
EATVLEFENYSEAFTALKAGKGDVLTTDNAILYGMAKQDSNYEVVGKIFTDEPYGIAVQK
CCEEEEECCHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCEEEEEEEECCCCCCEEEEC
GADDLTKEINSLLKDMKANGEYDKLYEKWIGQKQEK
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLKMKKLFTLIVFSCLFVLVVAGCGSKKDEAKETNTKQGGAVEQIKKRGKLVVGVKNDTN
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHCCCEEEEECCCCC
LFGLKNPSTGQVEGFDVDVAKALAKKILGDEKKLELKEVTSKTRIPLLKNGDIDAIIATM
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCEEEEEE
TITEERKKEVDFSDVYFKAGQSLLVKKGSNIKSIDDVKKGVKVLAVKGSTSTNNIRQKSP
EECHHHHHCCCHHHHHHHCCCEEEEECCCCCCHHHHHHCCEEEEEEECCCCCCCHHHCCC
EATVLEFENYSEAFTALKAGKGDVLTTDNAILYGMAKQDSNYEVVGKIFTDEPYGIAVQK
CCEEEEECCHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCEEEEEEEECCCCCCEEEEC
GADDLTKEINSLLKDMKANGEYDKLYEKWIGQKQEK
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 9384377 [H]