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The map label for this gene is proI [H]

Identifier: 218232312

GI number: 218232312

Start: 195252

End: 196013

Strand: Reverse

Name: proI [H]

Synonym: BCB4264_A0223

Alternate gene names: 218232312

Gene position: 196013-195252 (Counterclockwise)

Preceding gene: 218233870

Following gene: 218233093

Centisome position: 3.62

GC content: 38.98

Gene sequence:

>762_bases
ATGGCAGAAGCTATATTTTCCGGATTACTTAAAACAAGCAAAGAGTACATCGAAGAAATTATCGTCTCTAACCGAAGCAA
CATAGAAAAGCTAAAACAGTTACAACGTCGATATAATGTGTCTACTACAACAGATTGGAAACAGCATGTTACATCTGTTG
ATACGGTTGTTTTAGCAATGCCACCTTCTGCTCATGAAGAGTTATTAGCTGAGCTATCTCCGTTAATATCAAATCAACTA
GTCGTAACGGTAGCAGCTGGCATTGGACCATCTTATTTAGAAGCTAGACTCCCAAAAGGCACGCCTGTTGCTTGGATTAT
GCCTAATACAGCTGCTGAAATTGGCAAGTCTATCTCCTTATATACAATGGGACAATCAGTAAACGAGATACATCAAGAAA
CCCTGCAACTACTTTTAAAAGGTATTGGTACTTCGCAACTTTGTACTGAGGAAGAAGTTCATCAGCTTACTGCAGTTACT
GGAAGTGCACCAGCCTTCCTTTATTACTTTGCTGAAAGCTTAATTGAAGCAACAAAAAGTTATGGAGTTGATGAAGAAAC
CGCAAAACATCTTGTCATTCAAATGATTTCTGGCTCTGCTTCTATGCTTGAACAAACGCAAAATCCAGCTAACCTTCGCG
AACAAGTAACAACGCCAGGTGGTTCCACAGCTGAGGGTCTCAAAACTTTATACGAATATAATTTTTCAGAGGCAATTCAA
AAAGCAGTAGAAGCAACAAACAAAAAAGCTAGGGGGAAATAA

Upstream 100 bases:

>100_bases
TTCTCTTAAAGATGATATCGTTTTTATAACATATCTCTACTAGAAAGGAATAAAGGATATGCTAACTAAACATCGAATTT
TATTTATTGGTGCCGGTCGT

Downstream 100 bases:

>100_bases
TATGATTTTAACAAGTTTAAAAGACTATACAACATTACATAACGGTGTAAAAATGCCTTGGTTTGGTTTAGGTGTTTTTA
AAGTAGAAGATGGTTCAGAA

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase 2; P5CR 2 [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MAEAIFSGLLKTSKEYIEEIIVSNRSNIEKLKQLQRRYNVSTTTDWKQHVTSVDTVVLAMPPSAHEELLAELSPLISNQL
VVTVAAGIGPSYLEARLPKGTPVAWIMPNTAAEIGKSISLYTMGQSVNEIHQETLQLLLKGIGTSQLCTEEEVHQLTAVT
GSAPAFLYYFAESLIEATKSYGVDEETAKHLVIQMISGSASMLEQTQNPANLREQVTTPGGSTAEGLKTLYEYNFSEAIQ
KAVEATNKKARGK

Sequences:

>Translated_253_residues
MAEAIFSGLLKTSKEYIEEIIVSNRSNIEKLKQLQRRYNVSTTTDWKQHVTSVDTVVLAMPPSAHEELLAELSPLISNQL
VVTVAAGIGPSYLEARLPKGTPVAWIMPNTAAEIGKSISLYTMGQSVNEIHQETLQLLLKGIGTSQLCTEEEVHQLTAVT
GSAPAFLYYFAESLIEATKSYGVDEETAKHLVIQMISGSASMLEQTQNPANLREQVTTPGGSTAEGLKTLYEYNFSEAIQ
KAVEATNKKARGK
>Mature_252_residues
AEAIFSGLLKTSKEYIEEIIVSNRSNIEKLKQLQRRYNVSTTTDWKQHVTSVDTVVLAMPPSAHEELLAELSPLISNQLV
VTVAAGIGPSYLEARLPKGTPVAWIMPNTAAEIGKSISLYTMGQSVNEIHQETLQLLLKGIGTSQLCTEEEVHQLTAVTG
SAPAFLYYFAESLIEATKSYGVDEETAKHLVIQMISGSASMLEQTQNPANLREQVTTPGGSTAEGLKTLYEYNFSEAIQK
AVEATNKKARGK

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI198041662, Length=253, Percent_Identity=31.6205533596838, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI24797095, Length=224, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI24797097, Length=224, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI21361454, Length=224, Percent_Identity=28.125, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1786585, Length=257, Percent_Identity=26.4591439688716, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17569021, Length=253, Percent_Identity=28.4584980237154, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17540664, Length=270, Percent_Identity=30.3703703703704, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320861, Length=263, Percent_Identity=24.3346007604563, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358587, Length=252, Percent_Identity=28.1746031746032, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648116, Length=261, Percent_Identity=29.5019157088123, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24647700, Length=166, Percent_Identity=29.5180722891566, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304 [H]

Pfam domain/function: PF03807 F420_oxidored [H]

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 27604; Mature: 27473

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAIFSGLLKTSKEYIEEIIVSNRSNIEKLKQLQRRYNVSTTTDWKQHVTSVDTVVLAM
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEC
PPSAHEELLAELSPLISNQLVVTVAAGIGPSYLEARLPKGTPVAWIMPNTAAEIGKSISL
CCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCEEEECCCCHHHHCCCEEE
YTMGQSVNEIHQETLQLLLKGIGTSQLCTEEEVHQLTAVTGSAPAFLYYFAESLIEATKS
EECCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YGVDEETAKHLVIQMISGSASMLEQTQNPANLREQVTTPGGSTAEGLKTLYEYNFSEAIQ
CCCCHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHH
KAVEATNKKARGK
HHHHHHHHHCCCC
>Mature Secondary Structure 
AEAIFSGLLKTSKEYIEEIIVSNRSNIEKLKQLQRRYNVSTTTDWKQHVTSVDTVVLAM
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEC
PPSAHEELLAELSPLISNQLVVTVAAGIGPSYLEARLPKGTPVAWIMPNTAAEIGKSISL
CCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCEEEECCCCHHHHCCCEEE
YTMGQSVNEIHQETLQLLLKGIGTSQLCTEEEVHQLTAVTGSAPAFLYYFAESLIEATKS
EECCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YGVDEETAKHLVIQMISGSASMLEQTQNPANLREQVTTPGGSTAEGLKTLYEYNFSEAIQ
CCCCHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHH
KAVEATNKKARGK
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 11418582 [H]