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The map label for this gene is cwlH [H]

Identifier: 218231804

GI number: 218231804

Start: 893019

End: 894737

Strand: Reverse

Name: cwlH [H]

Synonym: BCB4264_A0925

Alternate gene names: 218231804

Gene position: 894737-893019 (Counterclockwise)

Preceding gene: 218236124

Following gene: 218232680

Centisome position: 16.51

GC content: 36.13

Gene sequence:

>1719_bases
GTGTTAAAAACAAATATGAAAAAGAAATTTTATAGTGTTTTAACTCTCTCTCTTGCATTACAAACTGTTCTTGCACCTAC
TTATTCATTTGCAGAGAGTGCTGAAAAATCTGCCGAAGTATATACGGAGGATCAAGTTTTTAAAAGTATTAAAGAACATG
TTAATGAGCATAGTACTCATGAAGAAGATATCGAGGTTACTGGCCAATTCCTACTATACACTTCAAAAAAACATTCATTA
TATACCGCGAATGATTTAAAAAACAAATCGAATATCGAGATTACAGAACAAGTCGTAACGGCGACAAAGAAAAAAGGAAA
TGCTTATTACATAACGACGTCAACTGGCGCTGGATGGATAGATAACAATGATAATAGCTTAGAAGTTCAAGAGATTCATA
AACTATCTAACCAAAAATTAATCATAAAAGAAGAAGCTTCCATACATGCACTTCCGTTCCAATCGTTTAAAGAAGAAGGA
AAACTAGAGCCACAAGTCATAACTCCTACTGAACAAGCTGGAAATTGGTTTAAAGTCCAAATAAATGAAACAGCAAAATG
GATTTATGCACCTTCTGCTACATTTGAAGGTACAAAAGCTTCATTATTAAAAAACAGTCGTACTGCTAAAAATAGTTTAT
TGAGATCGCCAGAAGCTCTACAAACAAACAATATTTATGGCGTTACATTTAAAGAAATGATTGTCCCAAAAGGAAATGAC
GATATTCGCCCTGGCTACCCGATGGTGCCTAAATATATTACGATTCACGAAACGGCTAATACAGCGAAAGGTGCTAATGC
TCTGAATCATGCAAAATTCTTAGATAATCAAGCACGCGGAACAGCTGATCGCGCTGCATCTTGGCATTTCACAGTAGATG
ATAAAGAAATTTATCAACATCTTCCAGTAAATGAAGTTGGATGGCATGCTGGAAATAAAACTGGAAACTATGAATCTATC
GGAATTGAAATTGCCGTTAATCAAGATGGAAACTATGAAAAAGCGGTAGAAAATGCACGTAAATTGGCAGCTTATTTAAT
GAACGACTTAAATATTTCTCTAGATAAGGTCCAAAAACACCAATTTTGGTCAGGAAAAAACTGCCCTGCGTTTATGATTC
AACGCGGACAATGGGATGCTTTCTTAAAAGGAACTGAAACATACTATAAGGAAAACCAAAAAAATCCCGTAACTGATGAC
ATTACAGGTGGATGGTATGAACAAGACATTCGTCAATTAGCAGCTAGAGGCATTATGCAAGGAGAAGGGAACGGCAAGTA
CTTCCCAGAACGTCTTGTAACGCGTGCTGAATTTGCTACATTAATTACTCGTGCTTTACAATTACCAAGTGGAAATGCTA
ATTTCACAGACTTAGAACAAGTACACCCATCTTTAAGAGACGGTATTAATCGTGCAGCAAGCGCAGGCATAATCCGCGGA
CGTGGTGATAATACATTTGATCCAAATACTACAATTACACGCGAAGAAGCTGTTATTATGATCGATCGTTCTCTAAAACA
CGCTGGTATTTTTGCAAAACAAGTTGAACTACCATTCGTAGACCAAAACTTAATTTACGCTAAAGAAGAAGTACAAAGAG
TGTATGGATATGGAATTGTAAAAGGTAATGAATTTAATCAATTCGTACCAAAAGGACCATCACAACGTGCTCACGCAGCT
GCATTTATAAACAGAATGCTTAGCGTAATTGAAGCTTAA

Upstream 100 bases:

>100_bases
ATGATTTTCTCTTGTTAAAATTTATTAGTGTTAAAATCGATACATAGTGAAAATACAATATAAAAAGGAGGACTGTTAAC
ACTAGATTCCACAATCTAAT

Downstream 100 bases:

>100_bases
CATATTTATCATTCCATATAGATATTTTCATTTTATCTGTATTCACTTAAAAGGCATCAGAGATTATATCTCTCTGATGC
CTTTTATACATCCGTTAACT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MLKTNMKKKFYSVLTLSLALQTVLAPTYSFAESAEKSAEVYTEDQVFKSIKEHVNEHSTHEEDIEVTGQFLLYTSKKHSL
YTANDLKNKSNIEITEQVVTATKKKGNAYYITTSTGAGWIDNNDNSLEVQEIHKLSNQKLIIKEEASIHALPFQSFKEEG
KLEPQVITPTEQAGNWFKVQINETAKWIYAPSATFEGTKASLLKNSRTAKNSLLRSPEALQTNNIYGVTFKEMIVPKGND
DIRPGYPMVPKYITIHETANTAKGANALNHAKFLDNQARGTADRAASWHFTVDDKEIYQHLPVNEVGWHAGNKTGNYESI
GIEIAVNQDGNYEKAVENARKLAAYLMNDLNISLDKVQKHQFWSGKNCPAFMIQRGQWDAFLKGTETYYKENQKNPVTDD
ITGGWYEQDIRQLAARGIMQGEGNGKYFPERLVTRAEFATLITRALQLPSGNANFTDLEQVHPSLRDGINRAASAGIIRG
RGDNTFDPNTTITREEAVIMIDRSLKHAGIFAKQVELPFVDQNLIYAKEEVQRVYGYGIVKGNEFNQFVPKGPSQRAHAA
AFINRMLSVIEA

Sequences:

>Translated_572_residues
MLKTNMKKKFYSVLTLSLALQTVLAPTYSFAESAEKSAEVYTEDQVFKSIKEHVNEHSTHEEDIEVTGQFLLYTSKKHSL
YTANDLKNKSNIEITEQVVTATKKKGNAYYITTSTGAGWIDNNDNSLEVQEIHKLSNQKLIIKEEASIHALPFQSFKEEG
KLEPQVITPTEQAGNWFKVQINETAKWIYAPSATFEGTKASLLKNSRTAKNSLLRSPEALQTNNIYGVTFKEMIVPKGND
DIRPGYPMVPKYITIHETANTAKGANALNHAKFLDNQARGTADRAASWHFTVDDKEIYQHLPVNEVGWHAGNKTGNYESI
GIEIAVNQDGNYEKAVENARKLAAYLMNDLNISLDKVQKHQFWSGKNCPAFMIQRGQWDAFLKGTETYYKENQKNPVTDD
ITGGWYEQDIRQLAARGIMQGEGNGKYFPERLVTRAEFATLITRALQLPSGNANFTDLEQVHPSLRDGINRAASAGIIRG
RGDNTFDPNTTITREEAVIMIDRSLKHAGIFAKQVELPFVDQNLIYAKEEVQRVYGYGIVKGNEFNQFVPKGPSQRAHAA
AFINRMLSVIEA
>Mature_572_residues
MLKTNMKKKFYSVLTLSLALQTVLAPTYSFAESAEKSAEVYTEDQVFKSIKEHVNEHSTHEEDIEVTGQFLLYTSKKHSL
YTANDLKNKSNIEITEQVVTATKKKGNAYYITTSTGAGWIDNNDNSLEVQEIHKLSNQKLIIKEEASIHALPFQSFKEEG
KLEPQVITPTEQAGNWFKVQINETAKWIYAPSATFEGTKASLLKNSRTAKNSLLRSPEALQTNNIYGVTFKEMIVPKGND
DIRPGYPMVPKYITIHETANTAKGANALNHAKFLDNQARGTADRAASWHFTVDDKEIYQHLPVNEVGWHAGNKTGNYESI
GIEIAVNQDGNYEKAVENARKLAAYLMNDLNISLDKVQKHQFWSGKNCPAFMIQRGQWDAFLKGTETYYKENQKNPVTDD
ITGGWYEQDIRQLAARGIMQGEGNGKYFPERLVTRAEFATLITRALQLPSGNANFTDLEQVHPSLRDGINRAASAGIIRG
RGDNTFDPNTTITREEAVIMIDRSLKHAGIFAKQVELPFVDQNLIYAKEEVQRVYGYGIVKGNEFNQFVPKGPSQRAHAA
AFINRMLSVIEA

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 64244; Mature: 64244

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS01072 SLH_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTNMKKKFYSVLTLSLALQTVLAPTYSFAESAEKSAEVYTEDQVFKSIKEHVNEHSTH
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEDIEVTGQFLLYTSKKHSLYTANDLKNKSNIEITEQVVTATKKKGNAYYITTSTGAGWI
CCCHHCCEEEEEEECCCCCEEEHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCC
DNNDNSLEVQEIHKLSNQKLIIKEEASIHALPFQSFKEEGKLEPQVITPTEQAGNWFKVQ
CCCCCCEEHHHHHHHCCCEEEEEECCCEEECCCHHHHHCCCCCCEEECCHHHCCCEEEEE
INETAKWIYAPSATFEGTKASLLKNSRTAKNSLLRSPEALQTNNIYGVTFKEMIVPKGND
ECCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHCCCCHHCCCCEEEEEHHHEECCCCCC
DIRPGYPMVPKYITIHETANTAKGANALNHAKFLDNQARGTADRAASWHFTVDDKEIYQH
CCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCHHHCCEEEEECCHHHHHHH
LPVNEVGWHAGNKTGNYESIGIEIAVNQDGNYEKAVENARKLAAYLMNDLNISLDKVQKH
CCCCHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QFWSGKNCPAFMIQRGQWDAFLKGTETYYKENQKNPVTDDITGGWYEQDIRQLAARGIMQ
HCCCCCCCCEEEEECCCCCCHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
GEGNGKYFPERLVTRAEFATLITRALQLPSGNANFTDLEQVHPSLRDGINRAASAGIIRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHHHHHHHHHCCEEEC
RGDNTFDPNTTITREEAVIMIDRSLKHAGIFAKQVELPFVDQNLIYAKEEVQRVYGYGIV
CCCCCCCCCCEEECCCEEEEEECCCHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCEEE
KGNEFNQFVPKGPSQRAHAAAFINRMLSVIEA
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKTNMKKKFYSVLTLSLALQTVLAPTYSFAESAEKSAEVYTEDQVFKSIKEHVNEHSTH
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEDIEVTGQFLLYTSKKHSLYTANDLKNKSNIEITEQVVTATKKKGNAYYITTSTGAGWI
CCCHHCCEEEEEEECCCCCEEEHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCC
DNNDNSLEVQEIHKLSNQKLIIKEEASIHALPFQSFKEEGKLEPQVITPTEQAGNWFKVQ
CCCCCCEEHHHHHHHCCCEEEEEECCCEEECCCHHHHHCCCCCCEEECCHHHCCCEEEEE
INETAKWIYAPSATFEGTKASLLKNSRTAKNSLLRSPEALQTNNIYGVTFKEMIVPKGND
ECCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHCCCCHHCCCCEEEEEHHHEECCCCCC
DIRPGYPMVPKYITIHETANTAKGANALNHAKFLDNQARGTADRAASWHFTVDDKEIYQH
CCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCHHHCCEEEEECCHHHHHHH
LPVNEVGWHAGNKTGNYESIGIEIAVNQDGNYEKAVENARKLAAYLMNDLNISLDKVQKH
CCCCHHCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QFWSGKNCPAFMIQRGQWDAFLKGTETYYKENQKNPVTDDITGGWYEQDIRQLAARGIMQ
HCCCCCCCCEEEEECCCCCCHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
GEGNGKYFPERLVTRAEFATLITRALQLPSGNANFTDLEQVHPSLRDGINRAASAGIIRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHHHHHHHHHHCCEEEC
RGDNTFDPNTTITREEAVIMIDRSLKHAGIFAKQVELPFVDQNLIYAKEEVQRVYGYGIV
CCCCCCCCCCEEECCCEEEEEECCCHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCEEE
KGNEFNQFVPKGPSQRAHAAAFINRMLSVIEA
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]