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The map label for this gene is pnp

Identifier: 21674467

GI number: 21674467

Start: 1559399

End: 1561600

Strand: Direct

Name: pnp

Synonym: CT1649

Alternate gene names: 21674467

Gene position: 1559399-1561600 (Clockwise)

Preceding gene: 21674466

Following gene: 21674468

Centisome position: 72.36

GC content: 60.49

Gene sequence:

>2202_bases
ATGTTTATCAAAAAAAAGATCGATCTTGGACACGGTAAGGTAATCACGATCGAAACCGGAAAAATGGCCAAACAGGCCGA
CGGCTCGGCGGTCGTCACAATGAACGACACCATGGTGCTCGCCACGGTGGTTTCAAGCAAAACACCTCCGTCGCCGAATC
AGGACTTCTTCCCGCTCCAGGTCGAATACCGCGAAAAATATTCCGCAGCCGGCAAGTTCCCCGGAGGCTTCTTCAAGCGC
GAAGGCCGCCCCTCCGAAAAGGAGATTCTCTCCGCCCGCCTGATCGACCGCGCCCTGCGCCCGCTCTTCCCTGACGGTTA
CTATCAGGAGACCCAGATCATCATCTCGGTCATCTCCTCCGACACGATCAATGACGCCGACGTGCTTGGCGGCATCGCCG
CTTCAGCCGCCATCATGGTCTCCGACATTCCGTTCGCCAACCCGATGTCCGAGGTTCGCGTCGGGCGCATCAACGGACTC
TTCATCGTCAACCCCGACATCAACGAGCTGGCGCAAAGCGACATGGACATCTGCATCGGCGGCACCGAGGACACCATCTG
TATGCTCGAGGGCGAGATGAAGGAGATCTCCGAAGCCGAGATGCTCGACGCCATCAAATTCGGCCACGACGCCATCAAGA
AAATTTGCGCCCTCCAGCGCGAACTCGCCGCAGAGGTAGCCAAGCCCAAACGGCCATTCTCTCCGACCGTCGCGCCGGAC
GAGCTGGTCAATTTCGTTGAAGAGCACTGCTCAGCCGAGCTGAAAGCTTTGGCCTACACCCCGCTCGCCAAGGAGGAGCG
CGCCGAAAAGACCAAAGCCATCTACACGCAAACCATCAGGAAAACCCTCACGCACTTCACCGACCGCGTCGGCCCCGATC
AGATCGAAGCCGATCCCACCAGCGCGTTCTGCCTGAACGAACACATGATCGAAGAGTGCATCCATGCCGTCGAAAAGAAG
GTGATGCGCCACATGATTCTCGATGACGGCAAGCGCCTCGACGGCCGTACACTCGAACAGGTTCGCCCGATCAGCATCGA
GCTGGGCCTCATTCCGAGGGCTCACGGTTCAGCGCTCTTCACTCGCGGCGAAACGCAGGCACTCGTCACGCTGACGCTGG
GCACCAAGAAGGACGCCCAGTCGGTCGATACGCTGACTGACGACAAGGACAAGCGGTTCATGCTGCACTACAACTTCCCA
CCCTTCTCAGTCGGCGAAATCGGCAGGGTCGGCGGTGCCGGACGGCGTGAAATCGGCCACGGCAACCTCGCCGAGCGCGC
CATCAAAATGGTGATGCCATCCGAGCAGGAGTTCCCCTACACCGTGCGCCTCGTCTCGGACATCCTCGAATCGAACGGTT
CGTCCTCGATGGCCTCGGTCTGCGGCGGCACGCTGGCCGCGATGGACGGTGGCATTCCGCTCAAGAAACCGGTCTCCGGC
ATCGCAATGGGCCTCATCAAGGAGGGCGACCGCTACGCCGTGCTCTCCGACATTCTCGGCAACGAGGATCATCTCGGCGA
CATGGACTTCAAGGTGGCAGGCACCCGTGACGGCATTACCGCCTGCCAGATGGACATCAAGATCGACGGCCTCGACTACC
ACATCCTCGAAACCGCGCTCGAGCAGGCTCGCAAAGGTCGCTTGCACATCCTCGACGTCATGGCCGAGGCGATTCCCGAG
TCGCGTGCAGACATCGGTAAATACGCACCGCGCCTCACCACCATCCAGATTCCGGTGGACGCCATCGGCATGGTGATCGG
CAAGGGCGGCGAAACCATCCGCAGCATCACCGAGGAGACCGGCGCAGAGATCAACATCGACGACGACGGCACGGTCACCA
TCGCCTGCTCCAGCCCCGAAGCCACCAAAGCGGCGGTCGAAACCATCAAGACCCTCGTCTCCAAGCCGGAGGTCGGCACA
ATCTACATGGGCAAGGTTCGCGACATCCGCGACGAGCTCGGCGCCTTCGTCGAGTTCCTGCCAAAAACCGACGGTCTGGT
GCACATCTCGGAGATTGCCCGCGAACGTATCGCCAAGGTGAGCGACGTGCTCAAGGTGGGTGACCGGATCAAGGTGAAGC
TGATCGACGTGCGCAAGGATCCCCGCACCGGCAAGACCAAGTTCGCCCTCTCGATCAAGGCGCTACTCGACACCGACCAG
CAAGCCGAAACGAACGGCGAAGCAAAGCCGGCAAGAGACTGA

Upstream 100 bases:

>100_bases
GACCGCGACCGGCATCAAGAGCCCTGATTTCCCCAGCGAAACGGCATCGCCCCGAAGGTCCAGCGAAGAGGTTCATCACT
TTTAACCACAACAGATTTCA

Downstream 100 bases:

>100_bases
ACGCAGCGAAACTGAAACCAAGGCAGTGCGGGGGAAATAACCCTGCACTGCCTTTTTTCATTACCGGCGCGGGATTATCT
TCCCGGCAACATTTTCGAAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MFIKKKIDLGHGKVITIETGKMAKQADGSAVVTMNDTMVLATVVSSKTPPSPNQDFFPLQVEYREKYSAAGKFPGGFFKR
EGRPSEKEILSARLIDRALRPLFPDGYYQETQIIISVISSDTINDADVLGGIAASAAIMVSDIPFANPMSEVRVGRINGL
FIVNPDINELAQSDMDICIGGTEDTICMLEGEMKEISEAEMLDAIKFGHDAIKKICALQRELAAEVAKPKRPFSPTVAPD
ELVNFVEEHCSAELKALAYTPLAKEERAEKTKAIYTQTIRKTLTHFTDRVGPDQIEADPTSAFCLNEHMIEECIHAVEKK
VMRHMILDDGKRLDGRTLEQVRPISIELGLIPRAHGSALFTRGETQALVTLTLGTKKDAQSVDTLTDDKDKRFMLHYNFP
PFSVGEIGRVGGAGRREIGHGNLAERAIKMVMPSEQEFPYTVRLVSDILESNGSSSMASVCGGTLAAMDGGIPLKKPVSG
IAMGLIKEGDRYAVLSDILGNEDHLGDMDFKVAGTRDGITACQMDIKIDGLDYHILETALEQARKGRLHILDVMAEAIPE
SRADIGKYAPRLTTIQIPVDAIGMVIGKGGETIRSITEETGAEINIDDDGTVTIACSSPEATKAAVETIKTLVSKPEVGT
IYMGKVRDIRDELGAFVEFLPKTDGLVHISEIARERIAKVSDVLKVGDRIKVKLIDVRKDPRTGKTKFALSIKALLDTDQ
QAETNGEAKPARD

Sequences:

>Translated_733_residues
MFIKKKIDLGHGKVITIETGKMAKQADGSAVVTMNDTMVLATVVSSKTPPSPNQDFFPLQVEYREKYSAAGKFPGGFFKR
EGRPSEKEILSARLIDRALRPLFPDGYYQETQIIISVISSDTINDADVLGGIAASAAIMVSDIPFANPMSEVRVGRINGL
FIVNPDINELAQSDMDICIGGTEDTICMLEGEMKEISEAEMLDAIKFGHDAIKKICALQRELAAEVAKPKRPFSPTVAPD
ELVNFVEEHCSAELKALAYTPLAKEERAEKTKAIYTQTIRKTLTHFTDRVGPDQIEADPTSAFCLNEHMIEECIHAVEKK
VMRHMILDDGKRLDGRTLEQVRPISIELGLIPRAHGSALFTRGETQALVTLTLGTKKDAQSVDTLTDDKDKRFMLHYNFP
PFSVGEIGRVGGAGRREIGHGNLAERAIKMVMPSEQEFPYTVRLVSDILESNGSSSMASVCGGTLAAMDGGIPLKKPVSG
IAMGLIKEGDRYAVLSDILGNEDHLGDMDFKVAGTRDGITACQMDIKIDGLDYHILETALEQARKGRLHILDVMAEAIPE
SRADIGKYAPRLTTIQIPVDAIGMVIGKGGETIRSITEETGAEINIDDDGTVTIACSSPEATKAAVETIKTLVSKPEVGT
IYMGKVRDIRDELGAFVEFLPKTDGLVHISEIARERIAKVSDVLKVGDRIKVKLIDVRKDPRTGKTKFALSIKALLDTDQ
QAETNGEAKPARD
>Mature_733_residues
MFIKKKIDLGHGKVITIETGKMAKQADGSAVVTMNDTMVLATVVSSKTPPSPNQDFFPLQVEYREKYSAAGKFPGGFFKR
EGRPSEKEILSARLIDRALRPLFPDGYYQETQIIISVISSDTINDADVLGGIAASAAIMVSDIPFANPMSEVRVGRINGL
FIVNPDINELAQSDMDICIGGTEDTICMLEGEMKEISEAEMLDAIKFGHDAIKKICALQRELAAEVAKPKRPFSPTVAPD
ELVNFVEEHCSAELKALAYTPLAKEERAEKTKAIYTQTIRKTLTHFTDRVGPDQIEADPTSAFCLNEHMIEECIHAVEKK
VMRHMILDDGKRLDGRTLEQVRPISIELGLIPRAHGSALFTRGETQALVTLTLGTKKDAQSVDTLTDDKDKRFMLHYNFP
PFSVGEIGRVGGAGRREIGHGNLAERAIKMVMPSEQEFPYTVRLVSDILESNGSSSMASVCGGTLAAMDGGIPLKKPVSG
IAMGLIKEGDRYAVLSDILGNEDHLGDMDFKVAGTRDGITACQMDIKIDGLDYHILETALEQARKGRLHILDVMAEAIPE
SRADIGKYAPRLTTIQIPVDAIGMVIGKGGETIRSITEETGAEINIDDDGTVTIACSSPEATKAAVETIKTLVSKPEVGT
IYMGKVRDIRDELGAFVEFLPKTDGLVHISEIARERIAKVSDVLKVGDRIKVKLIDVRKDPRTGKTKFALSIKALLDTDQ
QAETNGEAKPARD

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=729, Percent_Identity=38.2716049382716, Blast_Score=464, Evalue=1e-130,
Organism=Escherichia coli, GI145693187, Length=718, Percent_Identity=48.7465181058496, Blast_Score=658, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=725, Percent_Identity=32.8275862068966, Blast_Score=337, Evalue=2e-92,
Organism=Drosophila melanogaster, GI281362905, Length=727, Percent_Identity=37.2764786795048, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651641, Length=727, Percent_Identity=37.2764786795048, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651643, Length=727, Percent_Identity=37.2764786795048, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161079377, Length=669, Percent_Identity=36.7713004484305, Blast_Score=401, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CHLTE (Q8KBY3)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662532.1
- HSSP:   P05055
- ProteinModelPortal:   Q8KBY3
- GeneID:   1006135
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1649
- NMPDR:   fig|194439.1.peg.1626
- TIGR:   CT1649
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   CTEP194439:CT_1649-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 79939; Mature: 79939

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIKKKIDLGHGKVITIETGKMAKQADGSAVVTMNDTMVLATVVSSKTPPSPNQDFFPLQ
CCEEEEECCCCCEEEEEECCCCHHCCCCCEEEEECCCEEEEEECCCCCCCCCCCCEEEEE
VEYREKYSAAGKFPGGFFKREGRPSEKEILSARLIDRALRPLFPDGYYQETQIIISVISS
EHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
DTINDADVLGGIAASAAIMVSDIPFANPMSEVRVGRINGLFIVNPDINELAQSDMDICIG
CCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHEEEEECEEEEECCCHHHHHHCCCCEEEC
GTEDTICMLEGEMKEISEAEMLDAIKFGHDAIKKICALQRELAAEVAKPKRPFSPTVAPD
CCCCEEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
ELVNFVEEHCSAELKALAYTPLAKEERAEKTKAIYTQTIRKTLTHFTDRVGPDQIEADPT
HHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SAFCLNEHMIEECIHAVEKKVMRHMILDDGKRLDGRTLEQVRPISIELGLIPRAHGSALF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEECCCCCCCCEEE
TRGETQALVTLTLGTKKDAQSVDTLTDDKDKRFMLHYNFPPFSVGEIGRVGGAGRREIGH
ECCCCEEEEEEEECCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCC
GNLAERAIKMVMPSEQEFPYTVRLVSDILESNGSSSMASVCGGTLAAMDGGIPLKKPVSG
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCCHHH
IAMGLIKEGDRYAVLSDILGNEDHLGDMDFKVAGTRDGITACQMDIKIDGLDYHILETAL
HHHHHHCCCCCEEHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHH
EQARKGRLHILDVMAEAIPESRADIGKYAPRLTTIQIPVDAIGMVIGKGGETIRSITEET
HHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEEEECHHHHHHEECCCCHHHHHHHHHC
GAEINIDDDGTVTIACSSPEATKAAVETIKTLVSKPEVGTIYMGKVRDIRDELGAFVEFL
CCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHH
PKTDGLVHISEIARERIAKVSDVLKVGDRIKVKLIDVRKDPRTGKTKFALSIKALLDTDQ
CCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEEEEEHHHHCCCC
QAETNGEAKPARD
HHCCCCCCCCCCC
>Mature Secondary Structure
MFIKKKIDLGHGKVITIETGKMAKQADGSAVVTMNDTMVLATVVSSKTPPSPNQDFFPLQ
CCEEEEECCCCCEEEEEECCCCHHCCCCCEEEEECCCEEEEEECCCCCCCCCCCCEEEEE
VEYREKYSAAGKFPGGFFKREGRPSEKEILSARLIDRALRPLFPDGYYQETQIIISVISS
EHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
DTINDADVLGGIAASAAIMVSDIPFANPMSEVRVGRINGLFIVNPDINELAQSDMDICIG
CCCCHHHHHHHHHHHHEEEEECCCCCCCHHHHEEEEECEEEEECCCHHHHHHCCCCEEEC
GTEDTICMLEGEMKEISEAEMLDAIKFGHDAIKKICALQRELAAEVAKPKRPFSPTVAPD
CCCCEEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
ELVNFVEEHCSAELKALAYTPLAKEERAEKTKAIYTQTIRKTLTHFTDRVGPDQIEADPT
HHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SAFCLNEHMIEECIHAVEKKVMRHMILDDGKRLDGRTLEQVRPISIELGLIPRAHGSALF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEECCCCCCCCEEE
TRGETQALVTLTLGTKKDAQSVDTLTDDKDKRFMLHYNFPPFSVGEIGRVGGAGRREIGH
ECCCCEEEEEEEECCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCC
GNLAERAIKMVMPSEQEFPYTVRLVSDILESNGSSSMASVCGGTLAAMDGGIPLKKPVSG
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCCHHH
IAMGLIKEGDRYAVLSDILGNEDHLGDMDFKVAGTRDGITACQMDIKIDGLDYHILETAL
HHHHHHCCCCCEEHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHH
EQARKGRLHILDVMAEAIPESRADIGKYAPRLTTIQIPVDAIGMVIGKGGETIRSITEET
HHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEEEECHHHHHHEECCCCHHHHHHHHHC
GAEINIDDDGTVTIACSSPEATKAAVETIKTLVSKPEVGTIYMGKVRDIRDELGAFVEFL
CCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHH
PKTDGLVHISEIARERIAKVSDVLKVGDRIKVKLIDVRKDPRTGKTKFALSIKALLDTDQ
CCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEEEEEHHHHCCCC
QAETNGEAKPARD
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901