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The map label for this gene is carB2 [H]

Identifier: 21674410

GI number: 21674410

Start: 1500222

End: 1502078

Strand: Direct

Name: carB2 [H]

Synonym: CT1592

Alternate gene names: 21674410

Gene position: 1500222-1502078 (Clockwise)

Preceding gene: 21674406

Following gene: 21674411

Centisome position: 69.62

GC content: 60.31

Gene sequence:

>1857_bases
ATGACCACTCCACTCCCTGAACTCTCCGAACAGGCGCAGGCCCTCAGCCAGAAATTGCCCGCCTCGGCTATGAAAAAAGC
CAAGGAGCACGGCTTTTCCGATTTCCAGATTGCCACCATATTCCAGACCAGCGAAGCTGCGGTGCGCGAGCTGCGAAACC
ATTACGGCGTGATCTCGGTCTTCAAGACCGTCGACACCTGCGCGGCGGAGTTCGACGCCAGCACACCGTACCACTACTCG
ACCTACGACGAGGAAAACGAGTCGGTACGTTCCGATAAAAAGAAGGTGATCATCCTCGGCGGCGGCCCGAACCGCATCGG
CCAGGGCATCGAGTTCGACTACTGCTGCGTGCAGGCGGTCTTCGCGCTCCGCGAGTCGGGGTACGAAACCATCATGGTCA
ACTGCAACCCCGAGACCGTCTCGACCGACTACGACATCGCCGACAAGCTCTACTTTGAGCCGCTCACCTTCGAGGACGTC
ATCCGCATCATCGAGCACGAACAGCCGCTCGGCGTCATCGTCAGCTTCGGCGGCCAGACGCCGCTCAAGCTCTCGACCAA
GCTCGACAAGGCGGGTGTGCACATCCTCGGCACATCCTCGGACGGCATCGACCTTGCCGAAGACCGCAAGCGGTTCGGCG
CGCTGCTCGAAAAGCTCGACATTCCGCACCCCGAGTACGGCACGGCAACCACCTTCGCCGAAGCACAGGAGATCACCAAC
AGGATCGGTTACCCGGTACTTGTCAGGCCCAGCTACGTGCTCGGCGGACGCGCCATGAAGATCATCTACAACGACGACTC
GCTCAAGGAGTACGTCGATCAGGCGCTCTTCATCACCGAAAAATATCCGCTGCTCATCGACCGCTTCCTCGAAACCGCCG
TCGAGTTTGACATCGATGCGCTGGCCGACAGCACCGACTGCGTCATCAGCGGCATCATGCAGCACGTCGAGGCCGCCGGC
ATCCACAGCGGCGACTCCACCTCGATCCTGCCCTACTACAATATCGATCCGGCGGCCATCGCCACGATGAAAGAGTACAC
CCGCAAGCTCGCCGAGCACATTGGCGTGATCGGCCTGATGAACGTGCAGTACGCCGTACAGAATGGCAAGGTGTACGTCA
TTGAGGTCAACCCGCGCGCCAGCCGCACCGTGCCTTTCGTGGGAAAGGCCACGGCGATTCCGGTGGTCAAGATCGCCACG
CGCATCATGCTCGGAGAAAAGCTCTGCGACTTGCGCAAGGAGTTCAACCTGAAGGATTGCGACGAGCTGGGTCTGAAGCA
CATGGCCATCAAAGAGCCAGTCTTCCCGTTCTCGAAGTTTGTCAAGTCCGGCGTCTATCTCGGCCCGGAGATGCGCTCGA
CCGGCGAAGCGATGAGCCTTGCCGAATCCTTCCCGGAGGCGTTCGCCAAAGCCTACCAGGCGGCCAACATGCACCTGCCG
CTCTCCGGCACGGTCTTCATCAGCGTCAACGATCAAGACAAGAACCAGCGCATCATCAACATCGCCCGCGAACTCTACCG
CATGGACTTCGACCTGGTGGCGACCGCTGGCACCCACGCCTTTTTGACGCAGCACGGCATCGATTGCAAGAAAATCTTCA
AGGTCGGTGAAGAGGGACGGCCCAACATTTTCGACATCATCAAGCTCGGCAAGATCGACTTCGTGATCAACACGCCTCGC
GGTGAGCGGGCGCTGCACGACGAGGAGGCCATCGGCTCGGCCTCGGTGCAGAACGGCGTGCCGTTCGTCACCACCATCGA
AGCCGCTGAAGCCTCCGTGCGGGCGATAGACTGCATCCGCTGCCAGGAGTTCGGCGTCAAGAGCCTGCAGGAGTACGCCA
CCTATCGCGATCAATAA

Upstream 100 bases:

>100_bases
CGGACGCCTTTTTATTATACTCCGGCGGTTGTAAAGTACGGCCCTGAATTGTTACCTTTGGTGATGCTCTACCAATCATG
ATTTTCCAACACGACGCTGC

Downstream 100 bases:

>100_bases
TTTCCGCGACAAAGCGCCGGGCATTTTCACCGATGCCCGGCCTTTCGCTCTCCCTCGGCCCGGCGACATTCAGCACGCCG
ATTCCATGCGCCCAGATCCA

Product: carbamoyl-phosphate synthase, large subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain [H]

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MTTPLPELSEQAQALSQKLPASAMKKAKEHGFSDFQIATIFQTSEAAVRELRNHYGVISVFKTVDTCAAEFDASTPYHYS
TYDEENESVRSDKKKVIILGGGPNRIGQGIEFDYCCVQAVFALRESGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDV
IRIIEHEQPLGVIVSFGGQTPLKLSTKLDKAGVHILGTSSDGIDLAEDRKRFGALLEKLDIPHPEYGTATTFAEAQEITN
RIGYPVLVRPSYVLGGRAMKIIYNDDSLKEYVDQALFITEKYPLLIDRFLETAVEFDIDALADSTDCVISGIMQHVEAAG
IHSGDSTSILPYYNIDPAAIATMKEYTRKLAEHIGVIGLMNVQYAVQNGKVYVIEVNPRASRTVPFVGKATAIPVVKIAT
RIMLGEKLCDLRKEFNLKDCDELGLKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAESFPEAFAKAYQAANMHLP
LSGTVFISVNDQDKNQRIINIARELYRMDFDLVATAGTHAFLTQHGIDCKKIFKVGEEGRPNIFDIIKLGKIDFVINTPR
GERALHDEEAIGSASVQNGVPFVTTIEAAEASVRAIDCIRCQEFGVKSLQEYATYRDQ

Sequences:

>Translated_618_residues
MTTPLPELSEQAQALSQKLPASAMKKAKEHGFSDFQIATIFQTSEAAVRELRNHYGVISVFKTVDTCAAEFDASTPYHYS
TYDEENESVRSDKKKVIILGGGPNRIGQGIEFDYCCVQAVFALRESGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDV
IRIIEHEQPLGVIVSFGGQTPLKLSTKLDKAGVHILGTSSDGIDLAEDRKRFGALLEKLDIPHPEYGTATTFAEAQEITN
RIGYPVLVRPSYVLGGRAMKIIYNDDSLKEYVDQALFITEKYPLLIDRFLETAVEFDIDALADSTDCVISGIMQHVEAAG
IHSGDSTSILPYYNIDPAAIATMKEYTRKLAEHIGVIGLMNVQYAVQNGKVYVIEVNPRASRTVPFVGKATAIPVVKIAT
RIMLGEKLCDLRKEFNLKDCDELGLKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAESFPEAFAKAYQAANMHLP
LSGTVFISVNDQDKNQRIINIARELYRMDFDLVATAGTHAFLTQHGIDCKKIFKVGEEGRPNIFDIIKLGKIDFVINTPR
GERALHDEEAIGSASVQNGVPFVTTIEAAEASVRAIDCIRCQEFGVKSLQEYATYRDQ
>Mature_617_residues
TTPLPELSEQAQALSQKLPASAMKKAKEHGFSDFQIATIFQTSEAAVRELRNHYGVISVFKTVDTCAAEFDASTPYHYST
YDEENESVRSDKKKVIILGGGPNRIGQGIEFDYCCVQAVFALRESGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDVI
RIIEHEQPLGVIVSFGGQTPLKLSTKLDKAGVHILGTSSDGIDLAEDRKRFGALLEKLDIPHPEYGTATTFAEAQEITNR
IGYPVLVRPSYVLGGRAMKIIYNDDSLKEYVDQALFITEKYPLLIDRFLETAVEFDIDALADSTDCVISGIMQHVEAAGI
HSGDSTSILPYYNIDPAAIATMKEYTRKLAEHIGVIGLMNVQYAVQNGKVYVIEVNPRASRTVPFVGKATAIPVVKIATR
IMLGEKLCDLRKEFNLKDCDELGLKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAESFPEAFAKAYQAANMHLPL
SGTVFISVNDQDKNQRIINIARELYRMDFDLVATAGTHAFLTQHGIDCKKIFKVGEEGRPNIFDIIKLGKIDFVINTPRG
ERALHDEEAIGSASVQNGVPFVTTIEAAEASVRAIDCIRCQEFGVKSLQEYATYRDQ

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ATP-grasp domains [H]

Homologues:

Organism=Homo sapiens, GI170295797, Length=605, Percent_Identity=36.5289256198347, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI21361331, Length=605, Percent_Identity=36.5289256198347, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI169790915, Length=605, Percent_Identity=36.5289256198347, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI18105007, Length=613, Percent_Identity=34.910277324633, Blast_Score=361, Evalue=1e-99,
Organism=Escherichia coli, GI1786216, Length=581, Percent_Identity=48.7091222030981, Blast_Score=557, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI193204318, Length=596, Percent_Identity=34.5637583892617, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6322331, Length=591, Percent_Identity=36.2098138747885, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322569, Length=488, Percent_Identity=37.7049180327869, Blast_Score=336, Evalue=6e-93,
Organism=Drosophila melanogaster, GI24642586, Length=548, Percent_Identity=35.7664233576642, Blast_Score=351, Evalue=8e-97,
Organism=Drosophila melanogaster, GI45555749, Length=516, Percent_Identity=37.015503875969, Blast_Score=348, Evalue=8e-96,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006275
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; PF02142 MGS [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 68480; Mature: 68349

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50975 ATP_GRASP ; PS00962 RIBOSOMAL_S2_1 ; PS00866 CPSASE_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPLPELSEQAQALSQKLPASAMKKAKEHGFSDFQIATIFQTSEAAVRELRNHYGVISV
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHCCHHHH
FKTVDTCAAEFDASTPYHYSTYDEENESVRSDKKKVIILGGGPNRIGQGIEFDYCCVQAV
HHHHHHHHHHCCCCCCCEECCCCCCCHHHHCCCCEEEEECCCCHHHCCCCCHHHHHHHHH
FALRESGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDVIRIIEHEQPLGVIVSFGGQT
HHHHHCCCEEEEEECCCCCCCCCCCHHHHHEECCCCHHHHHHHHHCCCCCEEEEEECCCC
PLKLSTKLDKAGVHILGTSSDGIDLAEDRKRFGALLEKLDIPHPEYGTATTFAEAQEITN
CEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
RIGYPVLVRPSYVLGGRAMKIIYNDDSLKEYVDQALFITEKYPLLIDRFLETAVEFDIDA
HCCCCEEECCCEEECCEEEEEEECCHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCHHH
LADSTDCVISGIMQHVEAAGIHSGDSTSILPYYNIDPAAIATMKEYTRKLAEHIGVIGLM
HCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCEEEEE
NVQYAVQNGKVYVIEVNPRASRTVPFVGKATAIPVVKIATRIMLGEKLCDLRKEFNLKDC
EEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
DELGLKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAESFPEAFAKAYQAANMHLP
HHHCCHHHHHHCCCCHHHHHHHCCCEECCCHHCCHHHHHHHHHHHHHHHHHHHHCCEEEC
LSGTVFISVNDQDKNQRIINIARELYRMDFDLVATAGTHAFLTQHGIDCKKIFKVGEEGR
CCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHEEECCCHHHHHHCCCCHHHHHHCCCCCC
PNIFDIIKLGKIDFVINTPRGERALHDEEAIGSASVQNGVPFVTTIEAAEASVRAIDCIR
CCHHHHEEECCEEEEEECCCCCCCCCHHHHCCCCHHCCCCCEEEEEHHHHHHHHHHHHHH
CQEFGVKSLQEYATYRDQ
HHHHCHHHHHHHHHHCCC
>Mature Secondary Structure 
TTPLPELSEQAQALSQKLPASAMKKAKEHGFSDFQIATIFQTSEAAVRELRNHYGVISV
CCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHCCHHHH
FKTVDTCAAEFDASTPYHYSTYDEENESVRSDKKKVIILGGGPNRIGQGIEFDYCCVQAV
HHHHHHHHHHCCCCCCCEECCCCCCCHHHHCCCCEEEEECCCCHHHCCCCCHHHHHHHHH
FALRESGYETIMVNCNPETVSTDYDIADKLYFEPLTFEDVIRIIEHEQPLGVIVSFGGQT
HHHHHCCCEEEEEECCCCCCCCCCCHHHHHEECCCCHHHHHHHHHCCCCCEEEEEECCCC
PLKLSTKLDKAGVHILGTSSDGIDLAEDRKRFGALLEKLDIPHPEYGTATTFAEAQEITN
CEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
RIGYPVLVRPSYVLGGRAMKIIYNDDSLKEYVDQALFITEKYPLLIDRFLETAVEFDIDA
HCCCCEEECCCEEECCEEEEEEECCHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCHHH
LADSTDCVISGIMQHVEAAGIHSGDSTSILPYYNIDPAAIATMKEYTRKLAEHIGVIGLM
HCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCEEEEE
NVQYAVQNGKVYVIEVNPRASRTVPFVGKATAIPVVKIATRIMLGEKLCDLRKEFNLKDC
EEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
DELGLKHMAIKEPVFPFSKFVKSGVYLGPEMRSTGEAMSLAESFPEAFAKAYQAANMHLP
HHHCCHHHHHHCCCCHHHHHHHCCCEECCCHHCCHHHHHHHHHHHHHHHHHHHHCCEEEC
LSGTVFISVNDQDKNQRIINIARELYRMDFDLVATAGTHAFLTQHGIDCKKIFKVGEEGR
CCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHEEECCCHHHHHHCCCCHHHHHHCCCCCC
PNIFDIIKLGKIDFVINTPRGERALHDEEAIGSASVQNGVPFVTTIEAAEASVRAIDCIR
CCHHHHEEECCEEEEEECCCCCCCCCHHHHCCCCHHCCCCCEEEEEHHHHHHHHHHHHHH
CQEFGVKSLQEYATYRDQ
HHHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]