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The map label for this gene is pyrC

Identifier: 21672598

GI number: 21672598

Start: 369492

End: 370541

Strand: Direct

Name: pyrC

Synonym: BUsg322

Alternate gene names: 21672598

Gene position: 369492-370541 (Clockwise)

Preceding gene: 30410819

Following gene: 21672601

Centisome position: 57.6

GC content: 25.81

Gene sequence:

>1050_bases
ATGTCTAAATTTTTAAAAAAAATTAAAATTATAAAACCTGATGACTGGCATGTTCATTTAAGAGATAATGAAATTTTAAA
AAAAGTTTCTCAATACACTGGAACATTTTATAAACGTGCAATCATTATGCCTAATCTCGAAGAACCTATTACAAATTGTT
TTAGATCAATCTCTTATCGTCGTAGAATTTTAAATTCTATGACACCAAATACTGCATTTCAACCATTAATGATTTGTTAC
TTAACAGAAAAAACTTCTCCTGAAGAATTACAACAAGGTTTTTTTAAAAAAATTTTTGTAGGAGCAAAATTATATCCTCA
TTGTTCTACAACTAATTCAAAATACGGTATAAAAAATATTAACAATATCTATCATTTATTTAATATTATGGAAAAAATAA
AAATGCCATTACTTATTCATGGAGAAGAAAATAATTTAAATATCGACATATATGATAGAGAAGCAAAATTTATTGAAAAT
ACACTAAAACCATTACGTAAAAAATTTCCAGAATTAAAAATAATACTAGAACATATTACAACAGAAGAAGCAATATCGTA
CATTGAGGAATGTAATTCTTCATATTTAGCTGGTACTATTACACCTCATCACTTAATGTTAAACCGAAATAATATGTTTA
TTGATGGAATTCAACCACATCTTTATTGTTTACCTCTTTTAAAAAGAAAAAAACATCAAATAGCTTTGAGAAATGTCATA
TCAAGTGGTAGTAAAAATTTTTTTTTAGGCAGTGATACAGCTCCACACTTTCATAAAAATAAAATCAACACTTTTGGTTG
CGCAGGTATATTTAATGCTCCATCTTCTTTATTATGTTATGTTAGCGTTTTTGAAGAAATGAATGCATTACAGCATTTTC
AATCTTTTTGCTCCGAAAACGGACCTAACTTTTATAATATGCCAATTAATAAAGAAACCATTACACTTGTCAAAAAACCT
CATAAAATTTTAGAAAAAATTAATATTGGTAAAAATATTATTGTTCCCTTTTTAGCTGGGAAAAGACTAGATTGGTCTAT
TGAAAAGTAA

Upstream 100 bases:

>100_bases
TAGAATTAATTTTTAAAAAATAAATATATTTTTACATATAGAGTGTTTTAATAAAAAAATGTTAATATATAAATATTTTC
TACATTAATTAAAACTAAAA

Downstream 100 bases:

>100_bases
AAAATAAATTTTTTAATTTTCTAAATGACCATCAACACCATATGTGGTATTATTTTGGTTTAATCTATATTTTTGAAAAG
TGTTTAAAAAACGTTGATTT

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSKFLKKIKIIKPDDWHVHLRDNEILKKVSQYTGTFYKRAIIMPNLEEPITNCFRSISYRRRILNSMTPNTAFQPLMICY
LTEKTSPEELQQGFFKKIFVGAKLYPHCSTTNSKYGIKNINNIYHLFNIMEKIKMPLLIHGEENNLNIDIYDREAKFIEN
TLKPLRKKFPELKIILEHITTEEAISYIEECNSSYLAGTITPHHLMLNRNNMFIDGIQPHLYCLPLLKRKKHQIALRNVI
SSGSKNFFLGSDTAPHFHKNKINTFGCAGIFNAPSSLLCYVSVFEEMNALQHFQSFCSENGPNFYNMPINKETITLVKKP
HKILEKINIGKNIIVPFLAGKRLDWSIEK

Sequences:

>Translated_349_residues
MSKFLKKIKIIKPDDWHVHLRDNEILKKVSQYTGTFYKRAIIMPNLEEPITNCFRSISYRRRILNSMTPNTAFQPLMICY
LTEKTSPEELQQGFFKKIFVGAKLYPHCSTTNSKYGIKNINNIYHLFNIMEKIKMPLLIHGEENNLNIDIYDREAKFIEN
TLKPLRKKFPELKIILEHITTEEAISYIEECNSSYLAGTITPHHLMLNRNNMFIDGIQPHLYCLPLLKRKKHQIALRNVI
SSGSKNFFLGSDTAPHFHKNKINTFGCAGIFNAPSSLLCYVSVFEEMNALQHFQSFCSENGPNFYNMPINKETITLVKKP
HKILEKINIGKNIIVPFLAGKRLDWSIEK
>Mature_348_residues
SKFLKKIKIIKPDDWHVHLRDNEILKKVSQYTGTFYKRAIIMPNLEEPITNCFRSISYRRRILNSMTPNTAFQPLMICYL
TEKTSPEELQQGFFKKIFVGAKLYPHCSTTNSKYGIKNINNIYHLFNIMEKIKMPLLIHGEENNLNIDIYDREAKFIENT
LKPLRKKFPELKIILEHITTEEAISYIEECNSSYLAGTITPHHLMLNRNNMFIDGIQPHLYCLPLLKRKKHQIALRNVIS
SGSKNFFLGSDTAPHFHKNKINTFGCAGIFNAPSSLLCYVSVFEEMNALQHFQSFCSENGPNFYNMPINKETITLVKKPH
KILEKINIGKNIIVPFLAGKRLDWSIEK

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=349, Percent_Identity=50.1432664756447, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323452, Length=355, Percent_Identity=34.6478873239437, Blast_Score=178, Evalue=1e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_BUCAP (Q8K9L2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660665.1
- ProteinModelPortal:   Q8K9L2
- SMR:   Q8K9L2
- EnsemblBacteria:   EBBUCT00000000299
- GeneID:   1005527
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg322
- GeneTree:   EBGT00050000007733
- HOGENOM:   HBG628648
- OMA:   KESACGC
- ProtClustDB:   PRK05451
- BioCyc:   BAPH198804:BUSG322-MONOMER
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 40558; Mature: 40427

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFLKKIKIIKPDDWHVHLRDNEILKKVSQYTGTFYKRAIIMPNLEEPITNCFRSISYR
CCHHHHHCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHH
RRILNSMTPNTAFQPLMICYLTEKTSPEELQQGFFKKIFVGAKLYPHCSTTNSKYGIKNI
HHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCHH
NNIYHLFNIMEKIKMPLLIHGEENNLNIDIYDREAKFIENTLKPLRKKFPELKIILEHIT
HHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHH
TEEAISYIEECNSSYLAGTITPHHLMLNRNNMFIDGIQPHLYCLPLLKRKKHQIALRNVI
HHHHHHHHHHCCCCEEEECCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
SSGSKNFFLGSDTAPHFHKNKINTFGCAGIFNAPSSLLCYVSVFEEMNALQHFQSFCSEN
HCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GPNFYNMPINKETITLVKKPHKILEKINIGKNIIVPFLAGKRLDWSIEK
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SKFLKKIKIIKPDDWHVHLRDNEILKKVSQYTGTFYKRAIIMPNLEEPITNCFRSISYR
CHHHHHCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHH
RRILNSMTPNTAFQPLMICYLTEKTSPEELQQGFFKKIFVGAKLYPHCSTTNSKYGIKNI
HHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCHH
NNIYHLFNIMEKIKMPLLIHGEENNLNIDIYDREAKFIENTLKPLRKKFPELKIILEHIT
HHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHH
TEEAISYIEECNSSYLAGTITPHHLMLNRNNMFIDGIQPHLYCLPLLKRKKHQIALRNVI
HHHHHHHHHHCCCCEEEECCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
SSGSKNFFLGSDTAPHFHKNKINTFGCAGIFNAPSSLLCYVSVFEEMNALQHFQSFCSEN
HCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GPNFYNMPINKETITLVKKPHKILEKINIGKNIIVPFLAGKRLDWSIEK
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438