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The map label for this gene is aspS

Identifier: 21672582

GI number: 21672582

Start: 349957

End: 351711

Strand: Direct

Name: aspS

Synonym: BUsg306

Alternate gene names: 21672582

Gene position: 349957-351711 (Clockwise)

Preceding gene: 21672581

Following gene: 21672585

Centisome position: 54.56

GC content: 24.33

Gene sequence:

>1755_bases
ATGCGTACTAAATATTGTGGAAATATTAGAATAATTGATTTACATAAATCAGTGATTTTATGTGGTTGGGTACATAAAAT
AAGAAATTTTAGTCAATTTATTTTTATTGACATGAGAGATTGGACAGGTATTGTCCAACTTGTTTTTGAAAAAAAAAACA
ATAAAGTTTTTACAAAAGCTGTAAATTTAAAGAACGAATCTTGCATTCAAGTTATAGGAATTGTCAAAAAGAGAAACGCT
AACAATAATAATCTCGATACTGGAGAAATAGAAATATTAGTAAATAAAATAAAAGTTTTTAATATTTCAAAAAACCTGCC
ATTAGACTATTCTAACAATAGTAATGATGATATACGATTGAAATATCGTTACTTAGATCTTCGTCGTTCTGAATTATTAG
AAAATCTTAAAATAAGAAATAAAATCACTCATTTAATAAGAATTTTTATGGAAAATAAAAATTTCTTAGATATTGAGACA
CCCTTTCTGACAAAATCTACACCAGAAGGAGCTCGAGACTATTTAGTTCCTAGTAGAAATTATCCAGGCAATTTTTATGC
ATTACCTCAATCACCACAATTATTTAAACAAATATTAATGATTTCTGGTATTGATAAATATTATCAAATAGTTAAATGTT
TTCGTGATGAAGATTTACGTTCAGATCGACAACCAGAATTTACACAAATTGATATTGAAGCTTCTTTCGTAAGTTCTACT
AAAATTAGAAACTTGGTAGAAACATTAATAAAAAAAATTTGGCTTAAAGTTATTAATTATAATTTAAATAAATTTCCTAA
AATTTCTTTTTACGATTCTATGAAACGCTACGGATCAGATAAACCTGATTTAAGAAATCCAATGGAAATTGTTGATATTT
CTGATATTGTTATTGAAGAAAAAGTTGCATCATTTTTTCAAATTAATTTAAAGAAAAAAAATAGAATAGCTTTATTATGT
TTTGGTCAAGGTAATAAGATAAGTCAGAAAAAAATTGATGAATATTCTAATTATGTAAAAAAATTTGGTGCAAAAAAACT
ATTTTATATAAAAATAAATAAAATTGAAAATAGATTTCAAGATATTCAAAGTTCAATAAAAAATATTTTAGATAAAAATA
CTTTAGAAAACATTTTAAGAAAAACAAATGCAAAAAATGGAAATATTTTGTTTTTACTTGCTGATGAAGAAAAAATTGTT
AACAAATCTCTTGGAATGCTACGTATAAAATTAGGTAATGATTTTATATTTTTTAAAAAAAATACTTGGAAACCTGTTTG
GATTGTAGATTTTCCTATGTTTAAACAAAATTCTGATGGAAAATTTTCTTCTAATCATCATCCATTTACTGCTTTAAAAA
AAAATAATCAAAATAAACTTGAAAAAAATCCGAATCTTGCTATTTCTGACAGTTATGATTTAGTTATAAATGGTTATGAA
ATAGGTGGAGGTTCAGTACGTATACATGATGCTAAAATACAAAAAAAAGTTTTTAACATTATTGGAATTGAAAAACAATT
CCAAAGGGAAAAATTTGGTTTTTTGATAGAAGCACTAAAATATGGTCCGCCACCTCATGCAGGAATAGCTTTAGGATTAG
ATAGAATAGTCATGCTTTTAACAAATACTAATAATATTAGAGATGTAATAGCTTTTCCAAAAACAACGTCAGCAAATTGC
CTAATGACTGACTCTCCTAGTAAATTAAAAAAATCAATTTTAAATGAGTTAGGTATAAATATATTAAAGAAATAA

Upstream 100 bases:

>100_bases
TAAACTCCCAAAAAATATTTCAAAAAAATTTTTTATTATCTATTAATCATAAAGATTAAAAAAATTTTTTTTAAAATCAA
GCACATCTTGAAGAGAATTT

Downstream 100 bases:

>100_bases
AAATTTTTATATTAATTTTTTTATATTACTTAATAAGTAAACACAAGAAGAAAATAAAACAATAGATGGACTAGTTGGAA
CGTTGTAAAAAAAAGATAAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MRTKYCGNIRIIDLHKSVILCGWVHKIRNFSQFIFIDMRDWTGIVQLVFEKKNNKVFTKAVNLKNESCIQVIGIVKKRNA
NNNNLDTGEIEILVNKIKVFNISKNLPLDYSNNSNDDIRLKYRYLDLRRSELLENLKIRNKITHLIRIFMENKNFLDIET
PFLTKSTPEGARDYLVPSRNYPGNFYALPQSPQLFKQILMISGIDKYYQIVKCFRDEDLRSDRQPEFTQIDIEASFVSST
KIRNLVETLIKKIWLKVINYNLNKFPKISFYDSMKRYGSDKPDLRNPMEIVDISDIVIEEKVASFFQINLKKKNRIALLC
FGQGNKISQKKIDEYSNYVKKFGAKKLFYIKINKIENRFQDIQSSIKNILDKNTLENILRKTNAKNGNILFLLADEEKIV
NKSLGMLRIKLGNDFIFFKKNTWKPVWIVDFPMFKQNSDGKFSSNHHPFTALKKNNQNKLEKNPNLAISDSYDLVINGYE
IGGGSVRIHDAKIQKKVFNIIGIEKQFQREKFGFLIEALKYGPPPHAGIALGLDRIVMLLTNTNNIRDVIAFPKTTSANC
LMTDSPSKLKKSILNELGINILKK

Sequences:

>Translated_584_residues
MRTKYCGNIRIIDLHKSVILCGWVHKIRNFSQFIFIDMRDWTGIVQLVFEKKNNKVFTKAVNLKNESCIQVIGIVKKRNA
NNNNLDTGEIEILVNKIKVFNISKNLPLDYSNNSNDDIRLKYRYLDLRRSELLENLKIRNKITHLIRIFMENKNFLDIET
PFLTKSTPEGARDYLVPSRNYPGNFYALPQSPQLFKQILMISGIDKYYQIVKCFRDEDLRSDRQPEFTQIDIEASFVSST
KIRNLVETLIKKIWLKVINYNLNKFPKISFYDSMKRYGSDKPDLRNPMEIVDISDIVIEEKVASFFQINLKKKNRIALLC
FGQGNKISQKKIDEYSNYVKKFGAKKLFYIKINKIENRFQDIQSSIKNILDKNTLENILRKTNAKNGNILFLLADEEKIV
NKSLGMLRIKLGNDFIFFKKNTWKPVWIVDFPMFKQNSDGKFSSNHHPFTALKKNNQNKLEKNPNLAISDSYDLVINGYE
IGGGSVRIHDAKIQKKVFNIIGIEKQFQREKFGFLIEALKYGPPPHAGIALGLDRIVMLLTNTNNIRDVIAFPKTTSANC
LMTDSPSKLKKSILNELGINILKK
>Mature_584_residues
MRTKYCGNIRIIDLHKSVILCGWVHKIRNFSQFIFIDMRDWTGIVQLVFEKKNNKVFTKAVNLKNESCIQVIGIVKKRNA
NNNNLDTGEIEILVNKIKVFNISKNLPLDYSNNSNDDIRLKYRYLDLRRSELLENLKIRNKITHLIRIFMENKNFLDIET
PFLTKSTPEGARDYLVPSRNYPGNFYALPQSPQLFKQILMISGIDKYYQIVKCFRDEDLRSDRQPEFTQIDIEASFVSST
KIRNLVETLIKKIWLKVINYNLNKFPKISFYDSMKRYGSDKPDLRNPMEIVDISDIVIEEKVASFFQINLKKKNRIALLC
FGQGNKISQKKIDEYSNYVKKFGAKKLFYIKINKIENRFQDIQSSIKNILDKNTLENILRKTNAKNGNILFLLADEEKIV
NKSLGMLRIKLGNDFIFFKKNTWKPVWIVDFPMFKQNSDGKFSSNHHPFTALKKNNQNKLEKNPNLAISDSYDLVINGYE
IGGGSVRIHDAKIQKKVFNIIGIEKQFQREKFGFLIEALKYGPPPHAGIALGLDRIVMLLTNTNNIRDVIAFPKTTSANC
LMTDSPSKLKKSILNELGINILKK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=37.3956594323873, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI45439306, Length=232, Percent_Identity=30.1724137931034, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI5031815, Length=237, Percent_Identity=25.3164556962025, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI194272210, Length=237, Percent_Identity=25.3164556962025, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI226491502, Length=253, Percent_Identity=25.6916996047431, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788173, Length=586, Percent_Identity=52.0477815699659, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=290, Percent_Identity=23.1034482758621, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790571, Length=431, Percent_Identity=20.6496519721578, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=604, Percent_Identity=35.4304635761589, Blast_Score=334, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI71994340, Length=294, Percent_Identity=22.4489795918367, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=294, Percent_Identity=22.4489795918367, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=294, Percent_Identity=22.4489795918367, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=253, Percent_Identity=25.296442687747, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=631, Percent_Identity=34.3898573692552, Blast_Score=293, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6321807, Length=240, Percent_Identity=25, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323011, Length=257, Percent_Identity=27.2373540856031, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324256, Length=164, Percent_Identity=29.2682926829268, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=206, Percent_Identity=22.8155339805825, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319872, Length=265, Percent_Identity=24.9056603773585, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24584738, Length=589, Percent_Identity=34.46519524618, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI24640849, Length=239, Percent_Identity=24.6861924686192, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24640851, Length=239, Percent_Identity=24.6861924686192, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136276, Length=235, Percent_Identity=27.2340425531915, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_BUCAP (P81432)

Other databases:

- EMBL:   L43549
- EMBL:   AE013218
- RefSeq:   NP_660649.1
- ProteinModelPortal:   P81432
- SMR:   P81432
- EnsemblBacteria:   EBBUCT00000000499
- GeneID:   1005510
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg306
- GeneTree:   EBGT00050000007970
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   BAPH198804:BUSG306-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67825; Mature: 67825

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKYCGNIRIIDLHKSVILCGWVHKIRNFSQFIFIDMRDWTGIVQLVFEKKNNKVFTKA
CCCEEECCEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHEEECCCCEEEEEE
VNLKNESCIQVIGIVKKRNANNNNLDTGEIEILVNKIKVFNISKNLPLDYSNNSNDDIRL
ECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEE
KYRYLDLRRSELLENLKIRNKITHLIRIFMENKNFLDIETPFLTKSTPEGARDYLVPSRN
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCCCCCCC
YPGNFYALPQSPQLFKQILMISGIDKYYQIVKCFRDEDLRSDRQPEFTQIDIEASFVSST
CCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEHHHHHH
KIRNLVETLIKKIWLKVINYNLNKFPKISFYDSMKRYGSDKPDLRNPMEIVDISDIVIEE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHEEEHHHHHHHH
KVASFFQINLKKKNRIALLCFGQGNKISQKKIDEYSNYVKKFGAKKLFYIKINKIENRFQ
HHHHHEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHH
DIQSSIKNILDKNTLENILRKTNAKNGNILFLLADEEKIVNKSLGMLRIKLGNDFIFFKK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHCCCCEEEEEECCEEEEEEC
NTWKPVWIVDFPMFKQNSDGKFSSNHHPFTALKKNNQNKLEKNPNLAISDSYDLVINGYE
CCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCEEEECCCEEEEEEEE
IGGGSVRIHDAKIQKKVFNIIGIEKQFQREKFGFLIEALKYGPPPHAGIALGLDRIVMLL
ECCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHEEEEE
TNTNNIRDVIAFPKTTSANCLMTDSPSKLKKSILNELGINILKK
ECCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHCHHHHCC
>Mature Secondary Structure
MRTKYCGNIRIIDLHKSVILCGWVHKIRNFSQFIFIDMRDWTGIVQLVFEKKNNKVFTKA
CCCEEECCEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHEEECCCCEEEEEE
VNLKNESCIQVIGIVKKRNANNNNLDTGEIEILVNKIKVFNISKNLPLDYSNNSNDDIRL
ECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEE
KYRYLDLRRSELLENLKIRNKITHLIRIFMENKNFLDIETPFLTKSTPEGARDYLVPSRN
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHCCCCCCC
YPGNFYALPQSPQLFKQILMISGIDKYYQIVKCFRDEDLRSDRQPEFTQIDIEASFVSST
CCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEHHHHHH
KIRNLVETLIKKIWLKVINYNLNKFPKISFYDSMKRYGSDKPDLRNPMEIVDISDIVIEE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHEEEHHHHHHHH
KVASFFQINLKKKNRIALLCFGQGNKISQKKIDEYSNYVKKFGAKKLFYIKINKIENRFQ
HHHHHEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHH
DIQSSIKNILDKNTLENILRKTNAKNGNILFLLADEEKIVNKSLGMLRIKLGNDFIFFKK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHCCCCEEEEEECCEEEEEEC
NTWKPVWIVDFPMFKQNSDGKFSSNHHPFTALKKNNQNKLEKNPNLAISDSYDLVINGYE
CCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCEEEECCCEEEEEEEE
IGGGSVRIHDAKIQKKVFNIIGIEKQFQREKFGFLIEALKYGPPPHAGIALGLDRIVMLL
ECCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHEEEEE
TNTNNIRDVIAFPKTTSANCLMTDSPSKLKKSILNELGINILKK
ECCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438