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The map label for this gene is gapA

Identifier: 21672565

GI number: 21672565

Start: 328785

End: 329783

Strand: Direct

Name: gapA

Synonym: BUsg287

Alternate gene names: 21672565

Gene position: 328785-329783 (Clockwise)

Preceding gene: 21672564

Following gene: 21672568

Centisome position: 51.26

GC content: 29.93

Gene sequence:

>999_bases
ATGACTATTAAAATAGGTATTAATGGATTTGGTCGTATTGGACGTGTTTTATTTCGTCTAGCTCAAGAACGTGAAAATAT
TGAAGTTGTAGCAATAAACGATTTACTTGATCCTAAATATATAGCTTACATGTTAAAATATGATTCCACACATGGAAATT
TTAAAAAAGATATTGAAGTCAAAAATAATAATCTTATTATTAATGGGAAAAAAATTAGAATTACCTCAATAAAAGATCCT
GAAAAATTAATGTGGGACAAATTATTAATTGATGTAGTAATTGAATCTACTGGTCTTTTCTTAACAAAAGATACAGCTTA
CAAACATATTTTATCTGGTGCGAAAAAAGTTGTAATAACCGGACCATCAAAAGATGATATTCCAATGTTTGTCAGGGGTG
CTAATTTTGATAAATACAAAGGAGAAAAAATTGTATCTAATGCCTCTTGTACAACTAATTGTTTAGCACCTTTATCTAAA
GTAATAGATGATCATTTTGGTATTATTGAAGGACTGATGACTACTGTACATGCTAGTACAGCTACGCAAAAAATTGTTGA
TAGTGCTTCTAAAAAAGATTGGAGAGGTGGTCGAGGAGCATTGCAAAATATAATTCCTTCTTCTACAGGTGCAGCTGTTG
CTGTAGGCAAGGTTTTACCAAATTTAAACGGAAAATTAACAGGAATAGCTTTTCGAGTTCCTACCGCCAATGTATCCGTA
GTTGATTTAACAGTACGTTATAAAAAAACAGCAACATACAATGAAATTTGTGAAGTTGTTAAAAACGCTTCTGAACAGAA
AATGAAAGGAATTTTAGGATATACTGAAGATGAAGTAGTATCAACAGACTTTAATGGTAAAGAATTAACTTCGATTTTTG
ATGCTAAAGCTGGTTTGTCTTTAAATAAAAATTTTGCCAAATTAATTTCTTGGTATGATAATGAAACAGGTTATTCTAGT
AAGGTATTAGATTTAGTTGAACTAGTTGCTTTAAAATAA

Upstream 100 bases:

>100_bases
TCAAAGATAAATCCTAACAAAATATTAAAAGAATATTAAAATCATTTATTTTAAATAAAATATAATATTAATTATTAATA
ATTTAAAAAAAGGTGAAATA

Downstream 100 bases:

>100_bases
ATATAATTTTATTTTTAATATACATAAAATATTCAAATAGTTAGATGATTGTATATATAAAACATATTTTATTTAATTTT
TTTAAAATTGCAGTATTAAG

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTIKIGINGFGRIGRVLFRLAQERENIEVVAINDLLDPKYIAYMLKYDSTHGNFKKDIEVKNNNLIINGKKIRITSIKDP
EKLMWDKLLIDVVIESTGLFLTKDTAYKHILSGAKKVVITGPSKDDIPMFVRGANFDKYKGEKIVSNASCTTNCLAPLSK
VIDDHFGIIEGLMTTVHASTATQKIVDSASKKDWRGGRGALQNIIPSSTGAAVAVGKVLPNLNGKLTGIAFRVPTANVSV
VDLTVRYKKTATYNEICEVVKNASEQKMKGILGYTEDEVVSTDFNGKELTSIFDAKAGLSLNKNFAKLISWYDNETGYSS
KVLDLVELVALK

Sequences:

>Translated_332_residues
MTIKIGINGFGRIGRVLFRLAQERENIEVVAINDLLDPKYIAYMLKYDSTHGNFKKDIEVKNNNLIINGKKIRITSIKDP
EKLMWDKLLIDVVIESTGLFLTKDTAYKHILSGAKKVVITGPSKDDIPMFVRGANFDKYKGEKIVSNASCTTNCLAPLSK
VIDDHFGIIEGLMTTVHASTATQKIVDSASKKDWRGGRGALQNIIPSSTGAAVAVGKVLPNLNGKLTGIAFRVPTANVSV
VDLTVRYKKTATYNEICEVVKNASEQKMKGILGYTEDEVVSTDFNGKELTSIFDAKAGLSLNKNFAKLISWYDNETGYSS
KVLDLVELVALK
>Mature_331_residues
TIKIGINGFGRIGRVLFRLAQERENIEVVAINDLLDPKYIAYMLKYDSTHGNFKKDIEVKNNNLIINGKKIRITSIKDPE
KLMWDKLLIDVVIESTGLFLTKDTAYKHILSGAKKVVITGPSKDDIPMFVRGANFDKYKGEKIVSNASCTTNCLAPLSKV
IDDHFGIIEGLMTTVHASTATQKIVDSASKKDWRGGRGALQNIIPSSTGAAVAVGKVLPNLNGKLTGIAFRVPTANVSVV
DLTVRYKKTATYNEICEVVKNASEQKMKGILGYTEDEVVSTDFNGKELTSIFDAKAGLSLNKNFAKLISWYDNETGYSSK
VLDLVELVALK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7669492, Length=332, Percent_Identity=60.2409638554217, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI7657116, Length=330, Percent_Identity=57.5757575757576, Blast_Score=388, Evalue=1e-108,
Organism=Escherichia coli, GI1788079, Length=330, Percent_Identity=73.6363636363636, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1789295, Length=329, Percent_Identity=38.6018237082067, Blast_Score=247, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17534677, Length=336, Percent_Identity=58.9285714285714, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17534679, Length=336, Percent_Identity=58.3333333333333, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32566163, Length=336, Percent_Identity=57.4404761904762, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17568413, Length=336, Percent_Identity=57.4404761904762, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6322409, Length=330, Percent_Identity=63.6363636363636, Blast_Score=411, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322468, Length=330, Percent_Identity=62.4242424242424, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321631, Length=330, Percent_Identity=62.1212121212121, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI19922412, Length=324, Percent_Identity=62.3456790123457, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI85725000, Length=331, Percent_Identity=60.1208459214501, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI22023983, Length=331, Percent_Identity=60.1208459214501, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17933600, Length=331, Percent_Identity=59.8187311178248, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI18110149, Length=331, Percent_Identity=59.8187311178248, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): G3P_BUCAP (Q07234)

Other databases:

- EMBL:   U11045
- EMBL:   AE013218
- EMBL:   Z15146
- PIR:   I40069
- RefSeq:   NP_660632.1
- ProteinModelPortal:   Q07234
- SMR:   Q07234
- EnsemblBacteria:   EBBUCT00000000263
- GeneID:   1005491
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg287
- GeneTree:   EBGT00050000007914
- HOGENOM:   HBG571736
- OMA:   DLKWDAI
- ProtClustDB:   CLSK315868
- BioCyc:   BAPH198804:BUSG287-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 36485; Mature: 36353

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 150-150 BINDING 34-34 BINDING 78-78 BINDING 180-180 BINDING 232-232 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKIGINGFGRIGRVLFRLAQERENIEVVAINDLLDPKYIAYMLKYDSTHGNFKKDIEV
CEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHEEEEEEECCCCCCCEEEEEE
KNNNLIINGKKIRITSIKDPEKLMWDKLLIDVVIESTGLFLTKDTAYKHILSGAKKVVIT
ECCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCEEEEE
GPSKDDIPMFVRGANFDKYKGEKIVSNASCTTNCLAPLSKVIDDHFGIIEGLMTTVHAST
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATQKIVDSASKKDWRGGRGALQNIIPSSTGAAVAVGKVLPNLNGKLTGIAFRVPTANVSV
HHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEHHHHHCCCCCCEEEEEEECCCCCEEE
VDLTVRYKKTATYNEICEVVKNASEQKMKGILGYTEDEVVSTDFNGKELTSIFDAKAGLS
EEEEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCC
LNKNFAKLISWYDNETGYSSKVLDLVELVALK
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIKIGINGFGRIGRVLFRLAQERENIEVVAINDLLDPKYIAYMLKYDSTHGNFKKDIEV
EEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHEEEEEEECCCCCCCEEEEEE
KNNNLIINGKKIRITSIKDPEKLMWDKLLIDVVIESTGLFLTKDTAYKHILSGAKKVVIT
ECCEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCEEEEE
GPSKDDIPMFVRGANFDKYKGEKIVSNASCTTNCLAPLSKVIDDHFGIIEGLMTTVHAST
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATQKIVDSASKKDWRGGRGALQNIIPSSTGAAVAVGKVLPNLNGKLTGIAFRVPTANVSV
HHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCEEHHHHHCCCCCCEEEEEEECCCCCEEE
VDLTVRYKKTATYNEICEVVKNASEQKMKGILGYTEDEVVSTDFNGKELTSIFDAKAGLS
EEEEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCC
LNKNFAKLISWYDNETGYSSKVLDLVELVALK
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7765846; 12089438; 7763501