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The map label for this gene is frr

Identifier: 21672509

GI number: 21672509

Start: 258819

End: 259376

Strand: Direct

Name: frr

Synonym: BUsg228

Alternate gene names: 21672509

Gene position: 258819-259376 (Clockwise)

Preceding gene: 21672508

Following gene: 21672510

Centisome position: 40.35

GC content: 24.55

Gene sequence:

>558_bases
GTGATTAATGATATTTATATTAAAAGTAATGAAAAAATGAAAGTATGTATTAATAACTTTCAAATTCAAGTTAATAATGT
TCGTACTGGACGTGCTTCTCCAGAATTATTAAACAGTATCTATGTTGAATATTTTGGTTCTAAAGTTCCTCTACGTCAAA
TATCTAATGTCGTAGTTGAAAACTATCACACTCTTAAAATTAATATTTTTGATGATTCCAGTACATCTTTAATTAAAAAA
GCAATTTTAAATTCAAATCTTGACTTAAATCCTATTTTATATGGTAAAGATATTATTGTACCAATACCTGGATTAACAGA
AGAAAGAAGAACACATTTAATAAAAGTAATTCGTAATAATGCAGAAAATGCACGAATTTATATAAGAAACATTCGCAGAG
ATGCAAATGATAAGATAAAAAATTATTTAAAAAGTAAGATTATTAGCGAAGATAACGAGCATGCTGCACAAAATAAAATT
CAAACAATGACAGACGATTATATTAAGAAAATAGATAAAATATTATCGGAAAAAGAAAAAGATCTTATGAAATTTTGA

Upstream 100 bases:

>100_bases
TCTTTATACCGTATTGTTAAAGGTTATGATGAAGGTACTCTGATTAAATAAATTATTAACAAATATTGTAAATTAAAGAA
TATATTGTATAGGTACTCAT

Downstream 100 bases:

>100_bases
AGTTACATAGAATATTTTGTAAATCATTTTCTATATTTTATCGTATTATTTTTGTACAGTCATTTTAGCAATTCAATATT
ATTTATTAATATTAAAAATT

Product: ribosome recycling factor

Products: NA

Alternate protein names: RRF; Ribosome-releasing factor

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MINDIYIKSNEKMKVCINNFQIQVNNVRTGRASPELLNSIYVEYFGSKVPLRQISNVVVENYHTLKINIFDDSSTSLIKK
AILNSNLDLNPILYGKDIIVPIPGLTEERRTHLIKVIRNNAENARIYIRNIRRDANDKIKNYLKSKIISEDNEHAAQNKI
QTMTDDYIKKIDKILSEKEKDLMKF

Sequences:

>Translated_185_residues
MINDIYIKSNEKMKVCINNFQIQVNNVRTGRASPELLNSIYVEYFGSKVPLRQISNVVVENYHTLKINIFDDSSTSLIKK
AILNSNLDLNPILYGKDIIVPIPGLTEERRTHLIKVIRNNAENARIYIRNIRRDANDKIKNYLKSKIISEDNEHAAQNKI
QTMTDDYIKKIDKILSEKEKDLMKF
>Mature_185_residues
MINDIYIKSNEKMKVCINNFQIQVNNVRTGRASPELLNSIYVEYFGSKVPLRQISNVVVENYHTLKINIFDDSSTSLIKK
AILNSNLDLNPILYGKDIIVPIPGLTEERRTHLIKVIRNNAENARIYIRNIRRDANDKIKNYLKSKIISEDNEHAAQNKI
QTMTDDYIKKIDKILSEKEKDLMKF

Specific function: Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another

COG id: COG0233

COG function: function code J; Ribosome recycling factor

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RRF family

Homologues:

Organism=Homo sapiens, GI20270317, Length=177, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI291042689, Length=150, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1786368, Length=185, Percent_Identity=51.3513513513513, Blast_Score=207, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24661488, Length=155, Percent_Identity=27.741935483871, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): RRF_BUCAP (Q8K9S8)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660576.1
- ProteinModelPortal:   Q8K9S8
- SMR:   Q8K9S8
- EnsemblBacteria:   EBBUCT00000000410
- GeneID:   1005427
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg228
- GeneTree:   EBGT00050000007965
- HOGENOM:   HBG533975
- OMA:   YYGAETP
- ProtClustDB:   PRK00083
- BioCyc:   BAPH198804:BUSG228-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00040
- InterPro:   IPR002661
- InterPro:   IPR015998
- PANTHER:   PTHR20982
- PANTHER:   PTHR20982:SF3
- TIGRFAMs:   TIGR00496

Pfam domain/function: PF01765 RRF; SSF55194 Ribosome_recyc_fac

EC number: NA

Molecular weight: Translated: 21510; Mature: 21510

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINDIYIKSNEKMKVCINNFQIQVNNVRTGRASPELLNSIYVEYFGSKVPLRQISNVVVE
CCCEEEEECCCEEEEEEEEEEEEEECEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHC
NYHTLKINIFDDSSTSLIKKAILNSNLDLNPILYGKDIIVPIPGLTEERRTHLIKVIRNN
CEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHCC
AENARIYIRNIRRDANDKIKNYLKSKIISEDNEHAAQNKIQTMTDDYIKKIDKILSEKEK
CCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLMKF
HHHCC
>Mature Secondary Structure
MINDIYIKSNEKMKVCINNFQIQVNNVRTGRASPELLNSIYVEYFGSKVPLRQISNVVVE
CCCEEEEECCCEEEEEEEEEEEEEECEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHC
NYHTLKINIFDDSSTSLIKKAILNSNLDLNPILYGKDIIVPIPGLTEERRTHLIKVIRNN
CEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHCC
AENARIYIRNIRRDANDKIKNYLKSKIISEDNEHAAQNKIQTMTDDYIKKIDKILSEKEK
CCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLMKF
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438