Click here to switch to the map view.

The map label for this gene is tsf

Identifier: 21672507

GI number: 21672507

Start: 257146

End: 257949

Strand: Direct

Name: tsf

Synonym: BUsg226

Alternate gene names: 21672507

Gene position: 257146-257949 (Clockwise)

Preceding gene: 21672506

Following gene: 21672508

Centisome position: 40.09

GC content: 26.37

Gene sequence:

>804_bases
ATGAGTCATATTACTGCTTTACTAATTAAAGAGTTGAGATTACGTACCGGAGCAGGGTTTATGGAATGTAAACGTGCTCT
AATAGAAGAGAAGGGAGATATTGAGTTGTCAATTGATAATTTAAGAAAATCAGGACAAGCACGAGCTGAGAAAAAAATAC
ATAATATTACAAATCAAGGACTGATATTTGCAAAAATTAAAAATAAAATTGGTGCTATGGTTGAATTGAATTGCCAAACT
GATTTTGTTGCGAAAGATACTTTATTTTATTCATTAGGTAAAGAAATTATTTTAACAGCATTGTCAAAAAAAATAAAAAA
TATTGATGATATTAGAAATGTTTTTGAAATAAAGAAAACAGAATTAATTGTAAAAACAGGTGAAAATATAAAAATTAATC
GATTTAGTTTCATCGAAGGTGATAATATAATATCTTATATACATAGTGGTCGAATTGGTGTATTAGTAAGTGCTAGTAAT
TTAAATCAAGAAACACTAAAAAATATTGCTATGCATATTGCGGCAAGTAAACCTGAATATTTATCTCCAAATGACATATC
TGATTCTGTTTTTAAACGTGAATATGAAATTCAATTAGAATTAGCAAAAAAACAGAATAAATCTTCTGAAATACTAAAAA
AGATAGTTCAAGGTCGAATGAATAAATTTGTTAATGACATTTCATTAGTTGGTCAAAAGTTCATTATGGATAATACAAAA
ACAGTAGGTGCATTACTAAATGAATATAATGCGCGTATTATATCTTTTATTAGATTTGAAATCGGTGACAAAATATTTAA
TTGA

Upstream 100 bases:

>100_bases
ATGGTAATTCGTTTAGCGAAATGTTGTTAGATTCAGATAAAAAAATGAACATAGAATAATCATTTCATATTTTTAATTCA
AAAGAGAAGAGAAAATTATT

Downstream 100 bases:

>100_bases
TTTTAAAAGACTATTTTAAAAAACAAAATATATATTGATCAATTTACAAAAAGTAAAAAATATTTTTAATAAAAAATTTA
TTTAGGTTAAAAACAATATG

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSHITALLIKELRLRTGAGFMECKRALIEEKGDIELSIDNLRKSGQARAEKKIHNITNQGLIFAKIKNKIGAMVELNCQT
DFVAKDTLFYSLGKEIILTALSKKIKNIDDIRNVFEIKKTELIVKTGENIKINRFSFIEGDNIISYIHSGRIGVLVSASN
LNQETLKNIAMHIAASKPEYLSPNDISDSVFKREYEIQLELAKKQNKSSEILKKIVQGRMNKFVNDISLVGQKFIMDNTK
TVGALLNEYNARIISFIRFEIGDKIFN

Sequences:

>Translated_267_residues
MSHITALLIKELRLRTGAGFMECKRALIEEKGDIELSIDNLRKSGQARAEKKIHNITNQGLIFAKIKNKIGAMVELNCQT
DFVAKDTLFYSLGKEIILTALSKKIKNIDDIRNVFEIKKTELIVKTGENIKINRFSFIEGDNIISYIHSGRIGVLVSASN
LNQETLKNIAMHIAASKPEYLSPNDISDSVFKREYEIQLELAKKQNKSSEILKKIVQGRMNKFVNDISLVGQKFIMDNTK
TVGALLNEYNARIISFIRFEIGDKIFN
>Mature_266_residues
SHITALLIKELRLRTGAGFMECKRALIEEKGDIELSIDNLRKSGQARAEKKIHNITNQGLIFAKIKNKIGAMVELNCQTD
FVAKDTLFYSLGKEIILTALSKKIKNIDDIRNVFEIKKTELIVKTGENIKINRFSFIEGDNIISYIHSGRIGVLVSASNL
NQETLKNIAMHIAASKPEYLSPNDISDSVFKREYEIQLELAKKQNKSSEILKKIVQGRMNKFVNDISLVGQKFIMDNTKT
VGALLNEYNARIISFIRFEIGDKIFN

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Escherichia coli, GI1786366, Length=265, Percent_Identity=49.4339622641509, Blast_Score=284, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17561440, Length=295, Percent_Identity=26.1016949152542, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921466, Length=303, Percent_Identity=26.4026402640264, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_BUCAP (Q8K9S9)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660574.1
- ProteinModelPortal:   Q8K9S9
- SMR:   Q8K9S9
- EnsemblBacteria:   EBBUCT00000000049
- GeneID:   1005425
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg226
- GeneTree:   EBGT00050000007971
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   BAPH198804:BUSG226-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 30254; Mature: 30123

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHITALLIKELRLRTGAGFMECKRALIEEKGDIELSIDNLRKSGQARAEKKIHNITNQG
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHHHHHHHCCCC
LIFAKIKNKIGAMVELNCQTDFVAKDTLFYSLGKEIILTALSKKIKNIDDIRNVFEIKKT
EEEEEEHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ELIVKTGENIKINRFSFIEGDNIISYIHSGRIGVLVSASNLNQETLKNIAMHIAASKPEY
EEEEECCCCEEEEEEEEECCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCC
LSPNDISDSVFKREYEIQLELAKKQNKSSEILKKIVQGRMNKFVNDISLVGQKFIMDNTK
CCCCCCCHHHHHHHHHEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TVGALLNEYNARIISFIRFEIGDKIFN
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHITALLIKELRLRTGAGFMECKRALIEEKGDIELSIDNLRKSGQARAEKKIHNITNQG
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHHHHHHHCCCC
LIFAKIKNKIGAMVELNCQTDFVAKDTLFYSLGKEIILTALSKKIKNIDDIRNVFEIKKT
EEEEEEHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ELIVKTGENIKINRFSFIEGDNIISYIHSGRIGVLVSASNLNQETLKNIAMHIAASKPEY
EEEEECCCCEEEEEEEEECCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCC
LSPNDISDSVFKREYEIQLELAKKQNKSSEILKKIVQGRMNKFVNDISLVGQKFIMDNTK
CCCCCCCHHHHHHHHHEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TVGALLNEYNARIISFIRFEIGDKIFN
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438