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The map label for this gene is ackA

Identifier: 21672458

GI number: 21672458

Start: 189118

End: 190329

Strand: Direct

Name: ackA

Synonym: BUsg169

Alternate gene names: 21672458

Gene position: 189118-190329 (Clockwise)

Preceding gene: 21672456

Following gene: 21672459

Centisome position: 29.48

GC content: 27.06

Gene sequence:

>1212_bases
ATGGTAAAAATATTGAATAATTTAATTTTTGTATTGAATTGTGGCAGTTCTTCTGTAAAGTTTGCAATATTAAATCCTGA
TAATAAAAAGAAATATTTATCTGGTTTAGTAGAGTGTTTGTTTTTATCAAAAACATATATGAAATGGAAAGATTTAACAA
CAGAACATAAAAAATGTATCGGTTCTAATTTATCTCATAAAGATGCTTTAAACTTTATTTTTAATAAGTTTTTTTTGGAA
AAAAAAGATATATATAAAAATATAGTAGGTATAGGACATAGAGTAGTTCATGGAGGAAATAAAATAAAATCGTCGACTTT
AATAGATGATAACATTATTCAATTAATTCAAAAGGCTATTTCATTTGCTCCATTACATAATCCAGCAAATTTAATTGGTA
TCAAAACAGCCATAGAGAAATTTCCTATTTTTGCAAAAAAAAATGTAGCTGTTTTTGATACATCTTTTTATCAAAATATG
CCAGAAACTTCATTTTTATACGCGATACCATATTTTTTTTATAAAGAGCATCATATTCGAAGATATGGCGCTCATGGTAC
AAGTCATCATTATGTTGCTTCCGAAGTTGCTTTGATTTTAAATAAAAATTTTAATTCACTTAATGTTATTACGTGTCATT
TAGGTAATGGAGCTTCTGTTTCGGCAGTATGTAATGGAATATGCGTAGACACTTCTATGGGATTAACACCTTTAGAAGGA
TTAGTAATGGGTACTCGTAGTGGTGACATAGATCCTTCAATTATTTTTTTCATGTATCAAAAACTTGGTATGAGTATTGA
CGAAATTCATACAATACTTACAAAAGAGTCTGGTTTATTAGGTCTAAGTGGAATAAGTAGTGATTTTCGTTATTTTGAAA
AAAAATACTATTTTGAAAAAAAAGCGAGATTAGCTGTAGATATATTCTGTCATCGTTTATCTAAATATATTGCTTCTTAT
ATGTCTTTAATGGAAAATAATTTGGATGCTATAGTATTCACTGGAGGGATTGGCGAAAATGTGCCATTAATTAGAGAAAT
TACCTTATCAAAATTATTATTAATAGGTTTTAAAATAGATACTCAACGTAATCTTGGTATTAAAAATGGAAAACATGGAT
TAATTACTACAGATAAATCTCGTCCTGCATTTGTTATTCCGACAGATGAAGAATTAGCAATAGCTCAAGAAACTATTAAG
ATAATAAAATAA

Upstream 100 bases:

>100_bases
TTATTTTAAACATTATCTTTTATACATAGAGTTCATGATTATATATTAAAAATAATAATTGCAAAAGTTTATATATAAAA
TATAATCTTTTAATAAAGAA

Downstream 100 bases:

>100_bases
AATTAAAACTGTTTTATAGTATTTTCTACATTAAATATAAATAAAAAAAAATTATGTCACGAATTATAATGTTAATTCCT
TTAAACAAAAAAGTTAGTTT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MVKILNNLIFVLNCGSSSVKFAILNPDNKKKYLSGLVECLFLSKTYMKWKDLTTEHKKCIGSNLSHKDALNFIFNKFFLE
KKDIYKNIVGIGHRVVHGGNKIKSSTLIDDNIIQLIQKAISFAPLHNPANLIGIKTAIEKFPIFAKKNVAVFDTSFYQNM
PETSFLYAIPYFFYKEHHIRRYGAHGTSHHYVASEVALILNKNFNSLNVITCHLGNGASVSAVCNGICVDTSMGLTPLEG
LVMGTRSGDIDPSIIFFMYQKLGMSIDEIHTILTKESGLLGLSGISSDFRYFEKKYYFEKKARLAVDIFCHRLSKYIASY
MSLMENNLDAIVFTGGIGENVPLIREITLSKLLLIGFKIDTQRNLGIKNGKHGLITTDKSRPAFVIPTDEELAIAQETIK
IIK

Sequences:

>Translated_403_residues
MVKILNNLIFVLNCGSSSVKFAILNPDNKKKYLSGLVECLFLSKTYMKWKDLTTEHKKCIGSNLSHKDALNFIFNKFFLE
KKDIYKNIVGIGHRVVHGGNKIKSSTLIDDNIIQLIQKAISFAPLHNPANLIGIKTAIEKFPIFAKKNVAVFDTSFYQNM
PETSFLYAIPYFFYKEHHIRRYGAHGTSHHYVASEVALILNKNFNSLNVITCHLGNGASVSAVCNGICVDTSMGLTPLEG
LVMGTRSGDIDPSIIFFMYQKLGMSIDEIHTILTKESGLLGLSGISSDFRYFEKKYYFEKKARLAVDIFCHRLSKYIASY
MSLMENNLDAIVFTGGIGENVPLIREITLSKLLLIGFKIDTQRNLGIKNGKHGLITTDKSRPAFVIPTDEELAIAQETIK
IIK
>Mature_403_residues
MVKILNNLIFVLNCGSSSVKFAILNPDNKKKYLSGLVECLFLSKTYMKWKDLTTEHKKCIGSNLSHKDALNFIFNKFFLE
KKDIYKNIVGIGHRVVHGGNKIKSSTLIDDNIIQLIQKAISFAPLHNPANLIGIKTAIEKFPIFAKKNVAVFDTSFYQNM
PETSFLYAIPYFFYKEHHIRRYGAHGTSHHYVASEVALILNKNFNSLNVITCHLGNGASVSAVCNGICVDTSMGLTPLEG
LVMGTRSGDIDPSIIFFMYQKLGMSIDEIHTILTKESGLLGLSGISSDFRYFEKKYYFEKKARLAVDIFCHRLSKYIASY
MSLMENNLDAIVFTGGIGENVPLIREITLSKLLLIGFKIDTQRNLGIKNGKHGLITTDKSRPAFVIPTDEELAIAQETIK
IIK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=396, Percent_Identity=57.3232323232323, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI145693184, Length=398, Percent_Identity=39.9497487437186, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_BUCAP (Q8K9W6)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660525.1
- ProteinModelPortal:   Q8K9W6
- SMR:   Q8K9W6
- EnsemblBacteria:   EBBUCT00000000546
- GeneID:   1005366
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg169
- GeneTree:   EBGT00050000008196
- HOGENOM:   HBG285863
- OMA:   PHLKDKN
- ProtClustDB:   PRK00180
- BioCyc:   BAPH198804:BUSG169-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 45211; Mature: 45211

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKILNNLIFVLNCGSSSVKFAILNPDNKKKYLSGLVECLFLSKTYMKWKDLTTEHKKCI
CCEEECCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
GSNLSHKDALNFIFNKFFLEKKDIYKNIVGIGHRVVHGGNKIKSSTLIDDNIIQLIQKAI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCHHHHHHHHHHH
SFAPLHNPANLIGIKTAIEKFPIFAKKNVAVFDTSFYQNMPETSFLYAIPYFFYKEHHIR
HHCCCCCCHHHHHHHHHHHHCCCEECCCEEEEECHHHHCCCCCCEEEHHHHHHHHHHHHH
RYGAHGTSHHYVASEVALILNKNFNSLNVITCHLGNGASVSAVCNGICVDTSMGLTPLEG
HHCCCCCCCCHHHHHHHEEEECCCCCEEEEEEECCCCCCHHHHHCCEEEECCCCCCCCCC
LVMGTRSGDIDPSIIFFMYQKLGMSIDEIHTILTKESGLLGLSGISSDFRYFEKKYYFEK
EEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
KARLAVDIFCHRLSKYIASYMSLMENNLDAIVFTGGIGENVPLIREITLSKLLLIGFKID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHEEEEEC
TQRNLGIKNGKHGLITTDKSRPAFVIPTDEELAIAQETIKIIK
CCCCCCCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVKILNNLIFVLNCGSSSVKFAILNPDNKKKYLSGLVECLFLSKTYMKWKDLTTEHKKCI
CCEEECCEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
GSNLSHKDALNFIFNKFFLEKKDIYKNIVGIGHRVVHGGNKIKSSTLIDDNIIQLIQKAI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCHHHHHHHHHHH
SFAPLHNPANLIGIKTAIEKFPIFAKKNVAVFDTSFYQNMPETSFLYAIPYFFYKEHHIR
HHCCCCCCHHHHHHHHHHHHCCCEECCCEEEEECHHHHCCCCCCEEEHHHHHHHHHHHHH
RYGAHGTSHHYVASEVALILNKNFNSLNVITCHLGNGASVSAVCNGICVDTSMGLTPLEG
HHCCCCCCCCHHHHHHHEEEECCCCCEEEEEEECCCCCCHHHHHCCEEEECCCCCCCCCC
LVMGTRSGDIDPSIIFFMYQKLGMSIDEIHTILTKESGLLGLSGISSDFRYFEKKYYFEK
EEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHH
KARLAVDIFCHRLSKYIASYMSLMENNLDAIVFTGGIGENVPLIREITLSKLLLIGFKID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHEEEEEC
TQRNLGIKNGKHGLITTDKSRPAFVIPTDEELAIAQETIKIIK
CCCCCCCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438