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The map label for this gene is nuoB

Identifier: 21672438

GI number: 21672438

Start: 165598

End: 166269

Strand: Direct

Name: nuoB

Synonym: BUsg148

Alternate gene names: 21672438

Gene position: 165598-166269 (Clockwise)

Preceding gene: 21672437

Following gene: 21672439

Centisome position: 25.82

GC content: 31.85

Gene sequence:

>672_bases
ATGAATTATACTTTAACTAGAGCAGAAAGAAAAAGTCAGAGACATTATCCGAATGAGACAACTCAATCTACTATAGATCC
TATAGAAGGATATTTAAAGAAAAACATTCTTATGGGTAAAGTTACTAAATTACTTCATAAAATAGTGAATTGGGGTCGAA
AAAATTCTCTTTGGCCTTATAATTTTGGTTTATCTTGTTGTTATGTTGAAATGGTTACAGCATTTACTTCTGTACATGAT
GTTGCACGTTTCGGTTCTGAAGTATTAAGAGCCTCTCCAAGACAAGCAGATGTTATGGTAATTGCAGGTACACCATTTAT
TAAAATGGCTCCTGTTATTCAAAGATTATACGATCAAATGTTAGAACCAAAATGGGTTATTTCTATGGGTGCATGTGCTA
ATTCTGGTGGTATGTACGATATTTATTCTGTTGTACAAGGAGTAGATAAATTTTTACCAGTAGATATTTATATTCCTGGT
TGTCCACCAAGACCTGAAGCATATATACATGCCTTAACATTACTTCAAAAAACAATTAATGAAGAAAGAAGACCTTTATC
TTGGGTAGTTGGAGAACAAGGCATATACCGCAAGAAAATGTTATCAGAAAAAATTCAAAACAGAAAAAAAAGAATTTCTG
TTGTGAATCTTCCAACTTCAGAAAAGATATAA

Upstream 100 bases:

>100_bases
TTATTTAGTCCGAATTAAAGCTTTAAACTGGGTTACGTCATGATAATATCAAAAAAATTGACATTGATTATATTTAGGAT
TTAATTAGATGAAAGAAAAA

Downstream 100 bases:

>100_bases
TTTTTTTTAAAAAGAATATAAATTTTTCATACTACTAGATCGTATTTTTTGTAAATATATTATTAATATTAAAAATTTTT
AGGTTAATGAAAAAATGAGA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MNYTLTRAERKSQRHYPNETTQSTIDPIEGYLKKNILMGKVTKLLHKIVNWGRKNSLWPYNFGLSCCYVEMVTAFTSVHD
VARFGSEVLRASPRQADVMVIAGTPFIKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDIYIPG
CPPRPEAYIHALTLLQKTINEERRPLSWVVGEQGIYRKKMLSEKIQNRKKRISVVNLPTSEKI

Sequences:

>Translated_223_residues
MNYTLTRAERKSQRHYPNETTQSTIDPIEGYLKKNILMGKVTKLLHKIVNWGRKNSLWPYNFGLSCCYVEMVTAFTSVHD
VARFGSEVLRASPRQADVMVIAGTPFIKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDIYIPG
CPPRPEAYIHALTLLQKTINEERRPLSWVVGEQGIYRKKMLSEKIQNRKKRISVVNLPTSEKI
>Mature_223_residues
MNYTLTRAERKSQRHYPNETTQSTIDPIEGYLKKNILMGKVTKLLHKIVNWGRKNSLWPYNFGLSCCYVEMVTAFTSVHD
VARFGSEVLRASPRQADVMVIAGTPFIKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDIYIPG
CPPRPEAYIHALTLLQKTINEERRPLSWVVGEQGIYRKKMLSEKIQNRKKRISVVNLPTSEKI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=149, Percent_Identity=54.3624161073826, Blast_Score=172, Evalue=2e-43,
Organism=Escherichia coli, GI1788624, Length=224, Percent_Identity=70.5357142857143, Blast_Score=338, Evalue=2e-94,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=40.650406504065, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=41.4634146341463, Blast_Score=94, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=145, Percent_Identity=51.0344827586207, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI18859983, Length=145, Percent_Identity=53.1034482758621, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24642371, Length=145, Percent_Identity=53.1034482758621, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24651058, Length=145, Percent_Identity=54.4827586206897, Blast_Score=168, Evalue=3e-42,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_BUCAP (Q8K9Y6)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660505.1
- ProteinModelPortal:   Q8K9Y6
- SMR:   Q8K9Y6
- EnsemblBacteria:   EBBUCT00000000311
- GeneID:   1005965
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg148
- GeneTree:   EBGT00050000008096
- HOGENOM:   HBG553221
- OMA:   PRPEAYI
- ProtClustDB:   PRK06411
- BioCyc:   BAPH198804:BUSG148-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 25497; Mature: 25497

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTLTRAERKSQRHYPNETTQSTIDPIEGYLKKNILMGKVTKLLHKIVNWGRKNSLWPY
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NFGLSCCYVEMVTAFTSVHDVARFGSEVLRASPRQADVMVIAGTPFIKMAPVIQRLYDQM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
LEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDIYIPGCPPRPEAYIHALTLLQKTIN
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHH
EERRPLSWVVGEQGIYRKKMLSEKIQNRKKRISVVNLPTSEKI
HHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCC
>Mature Secondary Structure
MNYTLTRAERKSQRHYPNETTQSTIDPIEGYLKKNILMGKVTKLLHKIVNWGRKNSLWPY
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NFGLSCCYVEMVTAFTSVHDVARFGSEVLRASPRQADVMVIAGTPFIKMAPVIQRLYDQM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
LEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDIYIPGCPPRPEAYIHALTLLQKTIN
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHH
EERRPLSWVVGEQGIYRKKMLSEKIQNRKKRISVVNLPTSEKI
HHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12089438