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The map label for this gene is gspE [H]

Identifier: 21244929

GI number: 21244929

Start: 4962683

End: 4964434

Strand: Direct

Name: gspE [H]

Synonym: XAC4212

Alternate gene names: 21244929

Gene position: 4962683-4964434 (Clockwise)

Preceding gene: 21244926

Following gene: 21244931

Centisome position: 95.89

GC content: 68.89

Gene sequence:

>1752_bases
ATGTTCGACCCGGTGGCCGCGGCCCTGCTGGCCGACGGCATGGTGGTACCGCACGAACACGAGCGCGTGCAGTTCTCCGC
CAGCGGCGCGCGCACCGCCAGCGATGTGCATCCGCTGGTGCTGCTGGCCAATCTGAAGTTGCACGCCGCGCAGCCGCCGG
CCGGCGAGCTGGGCCTGGAACGGCTCACCGAATGGCTGGCCACCCGCACCGGGCTGCGCTATCTGCGCATTGATCCCACC
CGCATCGATGTGGCCGCGGTCACCGGCGTGGTCTCGCATGCGTATGCGCGCAGGCACCGCATCCTGCCGCTGGCGCTGGA
TGCCGAGCGCGTGCTGATCGCCACCAGCGAGCCGTTGGCGCTGGACTGGCTGGCCGACGTGCAACACCTGAGCCGGCGCC
GCATCGAGCTGGCGCTGATCAACCCGCTGGACCTGCATCGCTACACGATGGAGTTCTTCGGCGTCACCCAGTCGGTGCGC
GGCGCGCGCAGCGATGCGCGCAGCACCGAACCCAACGCCGGCCTGCCCAGTTTCGAGCAGCTGGTGGAACTGGGCCGCGC
CGGCGACGTCAATGCCGACGACCACCACATCGTGCATATCGTCGACTGGCTGCTGCAGTACGCCTACGAACAGCGGGCGA
GCGACATCCATCTGGAGCCGCGTCGCGAGGCCGGGCGCATGCGCTTCCGCATCGATGGCGTGCTGCACAAGGTGCTGGAA
GTGCCGCCCTCGGTGATGACCGCCGTGGTCAGCCGCATCAAGGTGCTCGGGCGCATGGATCTGGCCGAACGCCGCCGCCC
GCAGGATGGCCGCATCAAGACCCGCTCGCCGGGTGGGCGCGAGGTGGAAATGCGGCTGTCCACGATGCCCACCGCCTTCG
GCGAGAAGTGCGTGATGCGCATCTTCGACCCGGATTCGGCGTTCAAGAGCATCGAGCAGCTGGGTTTCAGCCCCGAAGAA
GCCACCGGCTGGAGCGCGCTGGTCGAGCGCCCGCACGGCATCGTGCTGGTTACCGGCCCCACCGGCTCGGGCAAGACCAC
CACGCTGTATTCCACGCTCAAACGCCTGGCCACGCCGGATGTGAACGTGTGCAGCGTGGAAGACCCGATCGAGATGATCG
CGCCGGAATTCAACCAGATGCAGGTGCAGCAGAACATCGACCTGGATTTCGCCAGCGGCGTGCGCACGCTGCTGCGCCAG
GACCCGGACATCATCATGATCGGCGAGATCCGCGACCTGGAAACCGCGCAGATGGCGGTGCAGGCCTCGCTCACCGGGCA
TCTGGTGCTGTCCACGCTGCACACCAACGATGCCGCCTCGGCAATCACCCGCCTGCTCGACCTGGGCGTGCCGCATTACC
TGGTGGCCTCCACCCTCAACGGCGTGCTGGCGCAGCGGCTGGTGCGCACGCTGTGCGTGCATTGCAAGCGCCCGCACACG
CTCAACGACGCCGACTGGGCGGCCATCCGCGAGCCCGGCGAGGCGCTGCCGGAACCGCTCAACGTGCACGCCCCGGTCGG
TTGCCTGGAATGCCGCCGCACCGGTTACCTGGGCCGGGTGGGCCTGTACGAACTGCTGCCGGTCACCCCGCGCCTGCGTA
CCCTCATCCGCCCGGATACGGAACTTGCCAGCTTCAGCCAGGCTGCCCGCGGCGAAGGCCTGCGCACGCTGCGCCGCACC
GGCCTGGAGAAGGTCGCCGCCGGGCTGACCACCATCGAAGAAGTGCTGTCGGTGCTGCCGCCGCGGGAGTAG

Upstream 100 bases:

>100_bases
CTTTGCCGCGCAGCACCGCTGCTGGGGAAGAACACGCATGGAACAGCGGCGCACCGCCGATCCGGATAACGCTGTGGCAT
TGCTGCCGCGCGGCCGGCTG

Downstream 100 bases:

>100_bases
ACGCGTCCTACGTCCTGGCGACCACGCCGCTTGCGCGTGCGTGATCGGTCGACGCCGCGAGGCGTCGATGGCCGGCAGGG
GCCGGTTAAAAACGTCTGTG

Product: general secretory pathway-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MFDPVAAALLADGMVVPHEHERVQFSASGARTASDVHPLVLLANLKLHAAQPPAGELGLERLTEWLATRTGLRYLRIDPT
RIDVAAVTGVVSHAYARRHRILPLALDAERVLIATSEPLALDWLADVQHLSRRRIELALINPLDLHRYTMEFFGVTQSVR
GARSDARSTEPNAGLPSFEQLVELGRAGDVNADDHHIVHIVDWLLQYAYEQRASDIHLEPRREAGRMRFRIDGVLHKVLE
VPPSVMTAVVSRIKVLGRMDLAERRRPQDGRIKTRSPGGREVEMRLSTMPTAFGEKCVMRIFDPDSAFKSIEQLGFSPEE
ATGWSALVERPHGIVLVTGPTGSGKTTTLYSTLKRLATPDVNVCSVEDPIEMIAPEFNQMQVQQNIDLDFASGVRTLLRQ
DPDIIMIGEIRDLETAQMAVQASLTGHLVLSTLHTNDAASAITRLLDLGVPHYLVASTLNGVLAQRLVRTLCVHCKRPHT
LNDADWAAIREPGEALPEPLNVHAPVGCLECRRTGYLGRVGLYELLPVTPRLRTLIRPDTELASFSQAARGEGLRTLRRT
GLEKVAAGLTTIEEVLSVLPPRE

Sequences:

>Translated_583_residues
MFDPVAAALLADGMVVPHEHERVQFSASGARTASDVHPLVLLANLKLHAAQPPAGELGLERLTEWLATRTGLRYLRIDPT
RIDVAAVTGVVSHAYARRHRILPLALDAERVLIATSEPLALDWLADVQHLSRRRIELALINPLDLHRYTMEFFGVTQSVR
GARSDARSTEPNAGLPSFEQLVELGRAGDVNADDHHIVHIVDWLLQYAYEQRASDIHLEPRREAGRMRFRIDGVLHKVLE
VPPSVMTAVVSRIKVLGRMDLAERRRPQDGRIKTRSPGGREVEMRLSTMPTAFGEKCVMRIFDPDSAFKSIEQLGFSPEE
ATGWSALVERPHGIVLVTGPTGSGKTTTLYSTLKRLATPDVNVCSVEDPIEMIAPEFNQMQVQQNIDLDFASGVRTLLRQ
DPDIIMIGEIRDLETAQMAVQASLTGHLVLSTLHTNDAASAITRLLDLGVPHYLVASTLNGVLAQRLVRTLCVHCKRPHT
LNDADWAAIREPGEALPEPLNVHAPVGCLECRRTGYLGRVGLYELLPVTPRLRTLIRPDTELASFSQAARGEGLRTLRRT
GLEKVAAGLTTIEEVLSVLPPRE
>Mature_583_residues
MFDPVAAALLADGMVVPHEHERVQFSASGARTASDVHPLVLLANLKLHAAQPPAGELGLERLTEWLATRTGLRYLRIDPT
RIDVAAVTGVVSHAYARRHRILPLALDAERVLIATSEPLALDWLADVQHLSRRRIELALINPLDLHRYTMEFFGVTQSVR
GARSDARSTEPNAGLPSFEQLVELGRAGDVNADDHHIVHIVDWLLQYAYEQRASDIHLEPRREAGRMRFRIDGVLHKVLE
VPPSVMTAVVSRIKVLGRMDLAERRRPQDGRIKTRSPGGREVEMRLSTMPTAFGEKCVMRIFDPDSAFKSIEQLGFSPEE
ATGWSALVERPHGIVLVTGPTGSGKTTTLYSTLKRLATPDVNVCSVEDPIEMIAPEFNQMQVQQNIDLDFASGVRTLLRQ
DPDIIMIGEIRDLETAQMAVQASLTGHLVLSTLHTNDAASAITRLLDLGVPHYLVASTLNGVLAQRLVRTLCVHCKRPHT
LNDADWAAIREPGEALPEPLNVHAPVGCLECRRTGYLGRVGLYELLPVTPRLRTLIRPDTELASFSQAARGEGLRTLRRT
GLEKVAAGLTTIEEVLSVLPPRE

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=382, Percent_Identity=44.2408376963351, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI1786296, Length=375, Percent_Identity=47.2, Blast_Score=335, Evalue=6e-93,
Organism=Escherichia coli, GI87082188, Length=169, Percent_Identity=42.0118343195266, Blast_Score=109, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 64394; Mature: 64394

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDPVAAALLADGMVVPHEHERVQFSASGARTASDVHPLVLLANLKLHAAQPPAGELGLE
CCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCEEECCCCCCHHHHHH
RLTEWLATRTGLRYLRIDPTRIDVAAVTGVVSHAYARRHRILPLALDAERVLIATSEPLA
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCH
LDWLADVQHLSRRRIELALINPLDLHRYTMEFFGVTQSVRGARSDARSTEPNAGLPSFEQ
HHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCHHH
LVELGRAGDVNADDHHIVHIVDWLLQYAYEQRASDIHLEPRREAGRMRFRIDGVLHKVLE
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
VPPSVMTAVVSRIKVLGRMDLAERRRPQDGRIKTRSPGGREVEMRLSTMPTAFGEKCVMR
CCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCCCEEEEEEHHCCCHHHHHHEEE
IFDPDSAFKSIEQLGFSPEEATGWSALVERPHGIVLVTGPTGSGKTTTLYSTLKRLATPD
EECCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCC
VNVCSVEDPIEMIAPEFNQMQVQQNIDLDFASGVRTLLRQDPDIIMIGEIRDLETAQMAV
CCEECCCCCHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
QASLTGHLVLSTLHTNDAASAITRLLDLGVPHYLVASTLNGVLAQRLVRTLCVHCKRPHT
HHHCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LNDADWAAIREPGEALPEPLNVHAPVGCLECRRTGYLGRVGLYELLPVTPRLRTLIRPDT
CCCCCHHHHCCCHHHCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHCCCH
ELASFSQAARGEGLRTLRRTGLEKVAAGLTTIEEVLSVLPPRE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFDPVAAALLADGMVVPHEHERVQFSASGARTASDVHPLVLLANLKLHAAQPPAGELGLE
CCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCEEECCCCCCHHHHHH
RLTEWLATRTGLRYLRIDPTRIDVAAVTGVVSHAYARRHRILPLALDAERVLIATSEPLA
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCH
LDWLADVQHLSRRRIELALINPLDLHRYTMEFFGVTQSVRGARSDARSTEPNAGLPSFEQ
HHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCHHH
LVELGRAGDVNADDHHIVHIVDWLLQYAYEQRASDIHLEPRREAGRMRFRIDGVLHKVLE
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
VPPSVMTAVVSRIKVLGRMDLAERRRPQDGRIKTRSPGGREVEMRLSTMPTAFGEKCVMR
CCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCCCEEEEEEHHCCCHHHHHHEEE
IFDPDSAFKSIEQLGFSPEEATGWSALVERPHGIVLVTGPTGSGKTTTLYSTLKRLATPD
EECCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCC
VNVCSVEDPIEMIAPEFNQMQVQQNIDLDFASGVRTLLRQDPDIIMIGEIRDLETAQMAV
CCEECCCCCHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
QASLTGHLVLSTLHTNDAASAITRLLDLGVPHYLVASTLNGVLAQRLVRTLCVHCKRPHT
HHHCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LNDADWAAIREPGEALPEPLNVHAPVGCLECRRTGYLGRVGLYELLPVTPRLRTLIRPDT
CCCCCHHHHCCCHHHCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHCCCH
ELASFSQAARGEGLRTLRRTGLEKVAAGLTTIEEVLSVLPPRE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]