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The map label for this gene is hemF

Identifier: 21244826

GI number: 21244826

Start: 4813368

End: 4814267

Strand: Direct

Name: hemF

Synonym: XAC4109

Alternate gene names: 21244826

Gene position: 4813368-4814267 (Clockwise)

Preceding gene: 21244825

Following gene: 21244828

Centisome position: 93.0

GC content: 64.44

Gene sequence:

>900_bases
ATGAACGAATTCGACCGTGTACGCGATTATCTGACCGACCTGCAGGACCGGATCTGCGCCGCTGTGGAAGCTGCCGACGG
CAAGGCACGCTTCGCCGAGGATCTTTGGAAGCGCGAAGAAGGCGGCGGTGGCCGGACCCGCATCCTGCGCGACGGCGCGG
TGTTCGAGCAGGCCGGCATCGGTTTTTCGGATGTGTCCGGTACGCGCCTGCCGCCGTCGGCCAGTGCGCACCGGCCCGAG
CTTGCCGGTGCGACCTGGCGTGCATGCGGCGTCTCGCTGGTGTTTCATCCGCACAATCCGTATATCCCGACCACGCACAT
GAACGTGCGTTACTTCCGCGCCGAGCGCGATGGCGAGGTGGTGGCAGCCTGGTTTGGCGGTGGCTTCGACCTGACCCCGT
TCTATCCCTTCGATGAAGACGTGCAGCACTGGCACCGCGTTGCGCAGGCGCTGTGCGTGCCGTTTGGACAAGAGCGCTAT
GCCGCGCACAAGCGTTGGTGCGACGAATATTTTTTCCTGCGCCACCGCAACGAAACGCGTGGCGTGGGCGGGCTGTTCTT
CGACGATCTGGGTCAGGACTTCGAGCGCGACTTTGCCTACCAGCGCGCAGTGGGCGATGGCTTTCTGGATGCCTACATAC
CCATCGTGCAGCGTCGCAAGGACACACCCTATGGCGAGCGCGAACGCGAGTTTCAGCTGTACCGTCGGGGGCGCTACGTG
GAATTCAATCTGGTCTACGACCGCGGCACCCTGTTCGGTCTGCAAAGCGGTGGGCGCGCCGAGAGCATCCTGATGAGTCT
GCCGCCGCGGGTGCGCTGGGAGTACGGCTTTACGCCGGAGCCAGGCAGCGCGGAAGCGCGCCTGGCCGATTACCTCATCC
CACGGGACTGGCTGGGTTGA

Upstream 100 bases:

>100_bases
TTGCGGAGGACACTGCACCAGCATCCCCGCATCGCACTGCGACAAACGGGCGCGCTTGCGCGGGATGCACCACGCGGAGT
GCACGGCTTAGAATTGGGAC

Downstream 100 bases:

>100_bases
TGAAGACGCCGCCGTAAGGCGTGTCGTCCTAGCGGACCTCCATGCAGGCTGTGGTCGTGGCCGATACGTTCCAGCGCGCG
TCGCGAGCAAGTGCGCTACT

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNEFDRVRDYLTDLQDRICAAVEAADGKARFAEDLWKREEGGGGRTRILRDGAVFEQAGIGFSDVSGTRLPPSASAHRPE
LAGATWRACGVSLVFHPHNPYIPTTHMNVRYFRAERDGEVVAAWFGGGFDLTPFYPFDEDVQHWHRVAQALCVPFGQERY
AAHKRWCDEYFFLRHRNETRGVGGLFFDDLGQDFERDFAYQRAVGDGFLDAYIPIVQRRKDTPYGEREREFQLYRRGRYV
EFNLVYDRGTLFGLQSGGRAESILMSLPPRVRWEYGFTPEPGSAEARLADYLIPRDWLG

Sequences:

>Translated_299_residues
MNEFDRVRDYLTDLQDRICAAVEAADGKARFAEDLWKREEGGGGRTRILRDGAVFEQAGIGFSDVSGTRLPPSASAHRPE
LAGATWRACGVSLVFHPHNPYIPTTHMNVRYFRAERDGEVVAAWFGGGFDLTPFYPFDEDVQHWHRVAQALCVPFGQERY
AAHKRWCDEYFFLRHRNETRGVGGLFFDDLGQDFERDFAYQRAVGDGFLDAYIPIVQRRKDTPYGEREREFQLYRRGRYV
EFNLVYDRGTLFGLQSGGRAESILMSLPPRVRWEYGFTPEPGSAEARLADYLIPRDWLG
>Mature_299_residues
MNEFDRVRDYLTDLQDRICAAVEAADGKARFAEDLWKREEGGGGRTRILRDGAVFEQAGIGFSDVSGTRLPPSASAHRPE
LAGATWRACGVSLVFHPHNPYIPTTHMNVRYFRAERDGEVVAAWFGGGFDLTPFYPFDEDVQHWHRVAQALCVPFGQERY
AAHKRWCDEYFFLRHRNETRGVGGLFFDDLGQDFERDFAYQRAVGDGFLDAYIPIVQRRKDTPYGEREREFQLYRRGRYV
EFNLVYDRGTLFGLQSGGRAESILMSLPPRVRWEYGFTPEPGSAEARLADYLIPRDWLG

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI41393599, Length=303, Percent_Identity=43.5643564356436, Blast_Score=245, Evalue=3e-65,
Organism=Escherichia coli, GI1788777, Length=294, Percent_Identity=64.6258503401361, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6320249, Length=305, Percent_Identity=43.6065573770492, Blast_Score=245, Evalue=7e-66,
Organism=Drosophila melanogaster, GI17864388, Length=308, Percent_Identity=44.1558441558442, Blast_Score=258, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM6_XANAC (Q8PF76)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644408.1
- ProteinModelPortal:   Q8PF76
- SMR:   Q8PF76
- GeneID:   1158180
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC4109
- NMPDR:   fig|190486.1.peg.4052
- HOGENOM:   HBG631180
- OMA:   HMNTRFV
- ProtClustDB:   PRK05330
- BioCyc:   XAXO190486:XAC4109-MONOMER
- BRENDA:   1.3.3.3
- GO:   GO:0005737
- HAMAP:   MF_00333
- InterPro:   IPR001260
- InterPro:   IPR018375
- Gene3D:   G3DSA:3.40.1500.10
- PANTHER:   PTHR10755
- PIRSF:   PIRSF000166
- PRINTS:   PR00073

Pfam domain/function: PF01218 Coprogen_oxidas; SSF102886 Coprogen_oxidas

EC number: =1.3.3.3

Molecular weight: Translated: 34383; Mature: 34383

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: ACT_SITE 106-106 BINDING 92-92

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFDRVRDYLTDLQDRICAAVEAADGKARFAEDLWKREEGGGGRTRILRDGAVFEQAGI
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCC
GFSDVSGTRLPPSASAHRPELAGATWRACGVSLVFHPHNPYIPTTHMNVRYFRAERDGEV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCCCE
VAAWFGGGFDLTPFYPFDEDVQHWHRVAQALCVPFGQERYAAHKRWCDEYFFLRHRNETR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GVGGLFFDDLGQDFERDFAYQRAVGDGFLDAYIPIVQRRKDTPYGEREREFQLYRRGRYV
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEE
EFNLVYDRGTLFGLQSGGRAESILMSLPPRVRWEYGFTPEPGSAEARLADYLIPRDWLG
EEEEEEECCEEEEECCCCCHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHCCHHCCC
>Mature Secondary Structure
MNEFDRVRDYLTDLQDRICAAVEAADGKARFAEDLWKREEGGGGRTRILRDGAVFEQAGI
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCC
GFSDVSGTRLPPSASAHRPELAGATWRACGVSLVFHPHNPYIPTTHMNVRYFRAERDGEV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCCCE
VAAWFGGGFDLTPFYPFDEDVQHWHRVAQALCVPFGQERYAAHKRWCDEYFFLRHRNETR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GVGGLFFDDLGQDFERDFAYQRAVGDGFLDAYIPIVQRRKDTPYGEREREFQLYRRGRYV
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEE
EFNLVYDRGTLFGLQSGGRAESILMSLPPRVRWEYGFTPEPGSAEARLADYLIPRDWLG
EEEEEEECCEEEEECCCCCHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217