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The map label for this gene is def

Identifier: 21244235

GI number: 21244235

Start: 4148183

End: 4148698

Strand: Reverse

Name: def

Synonym: XAC3510

Alternate gene names: 21244235

Gene position: 4148698-4148183 (Counterclockwise)

Preceding gene: 21244236

Following gene: 21244233

Centisome position: 80.16

GC content: 62.4

Gene sequence:

>516_bases
ATGATTCGCGAAATTATCCGCATGGGCGACAAGCGTCTGCTACGCGTGGCACCGCCGGTCACCAACCTGGGAAGCGCCGA
GTTGCACACGCTGGTGGCCGATATGTTCGAAACCATGGACGCGGCGCGTGGCGTCGGTCTGGCAGCACCGCAGATCGCGG
TGGATCTGCAATTGATGGTGTTCGGTTTCGACGCCAGCGAGCGCTATCCGGAAGCGCCCGCAGTGCCGCGCACGGCCCTG
GCCAATGCGCAGATCGAGCCGCTGTCGGAAGACATGGAAAACGGCTGGGAAGGCTGTCTGTCGATTCCCGGCCTGCGCGC
AGTGATTCCGCGCTATCGCTTCATTCGCTACCGCGGCTTCGCACCCGACGGCAGCCCGATCGAGCGCGACGCCGAAGGCT
TCCACGCACGCGTGGTGCAGCACGAATACGATAATCTGGTCGGTCGCCTGTATCCCTCGCGCATCGAGAATTTCGACACG
TTCGGTTTCGAAGACGTGCTGTCCTACGATCTCTAG

Upstream 100 bases:

>100_bases
GTTCACTGCAGCGTCTGCGTGCCGTTGCACCGCCTGCACCTGTTCGCTACCGGTCGTCAGCCGCTCTAGAATCCACGCCA
CGATCGTCAGGAGCACCCGC

Downstream 100 bases:

>100_bases
CAGGTCGTTCGCTGTGTCCATTGCGGCGTGGCTGCCGCCATGGACGGCAGCCATCTGTGCTGGATTTCGCCTCATTCAAG
ACGGCCATCAGCAAGCTGCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MIREIIRMGDKRLLRVAPPVTNLGSAELHTLVADMFETMDAARGVGLAAPQIAVDLQLMVFGFDASERYPEAPAVPRTAL
ANAQIEPLSEDMENGWEGCLSIPGLRAVIPRYRFIRYRGFAPDGSPIERDAEGFHARVVQHEYDNLVGRLYPSRIENFDT
FGFEDVLSYDL

Sequences:

>Translated_171_residues
MIREIIRMGDKRLLRVAPPVTNLGSAELHTLVADMFETMDAARGVGLAAPQIAVDLQLMVFGFDASERYPEAPAVPRTAL
ANAQIEPLSEDMENGWEGCLSIPGLRAVIPRYRFIRYRGFAPDGSPIERDAEGFHARVVQHEYDNLVGRLYPSRIENFDT
FGFEDVLSYDL
>Mature_171_residues
MIREIIRMGDKRLLRVAPPVTNLGSAELHTLVADMFETMDAARGVGLAAPQIAVDLQLMVFGFDASERYPEAPAVPRTAL
ANAQIEPLSEDMENGWEGCLSIPGLRAVIPRYRFIRYRGFAPDGSPIERDAEGFHARVVQHEYDNLVGRLYPSRIENFDT
FGFEDVLSYDL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=174, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=9e-16,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=41.6107382550336, Blast_Score=103, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24645728, Length=177, Percent_Identity=28.8135593220339, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=28.9308176100629, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF1_XANAC (Q8PGV2)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643817.1
- ProteinModelPortal:   Q8PGV2
- SMR:   Q8PGV2
- GeneID:   1157581
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3510
- NMPDR:   fig|190486.1.peg.3461
- HOGENOM:   HBG665227
- OMA:   HNERYPD
- ProtClustDB:   PRK12846
- BioCyc:   XAXO190486:XAC3510-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19142; Mature: 19142

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREIIRMGDKRLLRVAPPVTNLGSAELHTLVADMFETMDAARGVGLAAPQIAVDLQLMV
CHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEE
FGFDASERYPEAPAVPRTALANAQIEPLSEDMENGWEGCLSIPGLRAVIPRYRFIRYRGF
EECCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCC
APDGSPIERDAEGFHARVVQHEYDNLVGRLYPSRIENFDTFGFEDVLSYDL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MIREIIRMGDKRLLRVAPPVTNLGSAELHTLVADMFETMDAARGVGLAAPQIAVDLQLMV
CHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEE
FGFDASERYPEAPAVPRTALANAQIEPLSEDMENGWEGCLSIPGLRAVIPRYRFIRYRGF
EECCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCC
APDGSPIERDAEGFHARVVQHEYDNLVGRLYPSRIENFDTFGFEDVLSYDL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217