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The map label for this gene is rihA [H]

Identifier: 21244115

GI number: 21244115

Start: 3996110

End: 3997048

Strand: Reverse

Name: rihA [H]

Synonym: XAC3390

Alternate gene names: 21244115

Gene position: 3997048-3996110 (Counterclockwise)

Preceding gene: 21244116

Following gene: 21244114

Centisome position: 77.23

GC content: 67.09

Gene sequence:

>939_bases
ATGACAAAAAAGATACCGCTGCTGATCGACACGGACCCCGGCGTGGACGACGCCTTGGCGCTGCTGATGGCCTTCAACGA
CAACCGCCATGAGGTCGTCGGCCTGACCATCGCCGCCGGCAACGTGGGCTTGGAGCACACCGTGCGCAACGCCTTGAAGG
TCTGCGAGATCGCTGGCCGTCCCGATGTGCCGGTCTACGCCGGCTGCCCGCAGCCATTGCTGCACCCGTCGGTGGATGCG
GCACACGTGCACGGTCTCGACGGATTCGGCGATGTGGGCCTGGCACCGGCCAAGCGCACCGCCGAGGCCGAACACGCCGC
GCTCGCCATCCTGCGTCTGTCGCACGAACACGCCGGCAAGCTGCTGCTGGTGGCGCTCGGTCCACTGACCAATCTTGCCC
TGGCACTCACTCTGGACCCGACCCTCCCGCAACGCGTGGCGCGGCTGGTGGTGATGGGCGGGGCGTTGACCGGGCACGGC
AATATCACCGCCGCGGCCGAGTTCAACATCGGGTTCGACCCGGAAGCGGCGCATATCGTGTTCCGAGGTTTTCCGCAGTT
CGACGTGGCCGATTGGGAAGCCACCATTGCGCACGGGCTACTGCACCGGGACGTGGAGCACTGGCTGGCGGCCGATTCTG
CCCGCGCCCGGTTCTACGAGGATATCTCGCGCAAGACCCGGCTGTGGTCCGAAGACAGCCGCGGCGAGTACTGGTATGCC
GCCGACGCGCTGGCGATGGCTTTCGCCTTGCACCCGGAAGGCGCGCAGCGGCTGGAGCAGCGCCCGGTACACATCGAACT
GTCCGGTGCGCATACCCGTGGCATGACCCTGGTGGACTGGAACCGGCAGGGCGGGGCGCCCGACAATGCCAATCTGCTGC
TGGCCTATGACATCCAGCAGTTCTACCGGCTGGTAGGGGCGGCCCTGGCGGCGAACTGA

Upstream 100 bases:

>100_bases
AAGCAAAGGCTGCTGCCCCTGTGCAACCTGGGCGTTCGCGTGACCGGCCGATCCTTGCGAAGATGCGGCAACGAATCCCG
AATGCCCGTTCCCGAATCCC

Downstream 100 bases:

>100_bases
GGCGATCTTGCACCGGCTGCCTGGGCGCAGCTATAATGCTCGGCTTATCCAGGCAGCCCGGCGCCAAGTCCATGAAAGAT
AACGTTCATCCCAACTACAA

Product: inosine-uridine preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTKKIPLLIDTDPGVDDALALLMAFNDNRHEVVGLTIAAGNVGLEHTVRNALKVCEIAGRPDVPVYAGCPQPLLHPSVDA
AHVHGLDGFGDVGLAPAKRTAEAEHAALAILRLSHEHAGKLLLVALGPLTNLALALTLDPTLPQRVARLVVMGGALTGHG
NITAAAEFNIGFDPEAAHIVFRGFPQFDVADWEATIAHGLLHRDVEHWLAADSARARFYEDISRKTRLWSEDSRGEYWYA
ADALAMAFALHPEGAQRLEQRPVHIELSGAHTRGMTLVDWNRQGGAPDNANLLLAYDIQQFYRLVGAALAAN

Sequences:

>Translated_312_residues
MTKKIPLLIDTDPGVDDALALLMAFNDNRHEVVGLTIAAGNVGLEHTVRNALKVCEIAGRPDVPVYAGCPQPLLHPSVDA
AHVHGLDGFGDVGLAPAKRTAEAEHAALAILRLSHEHAGKLLLVALGPLTNLALALTLDPTLPQRVARLVVMGGALTGHG
NITAAAEFNIGFDPEAAHIVFRGFPQFDVADWEATIAHGLLHRDVEHWLAADSARARFYEDISRKTRLWSEDSRGEYWYA
ADALAMAFALHPEGAQRLEQRPVHIELSGAHTRGMTLVDWNRQGGAPDNANLLLAYDIQQFYRLVGAALAAN
>Mature_311_residues
TKKIPLLIDTDPGVDDALALLMAFNDNRHEVVGLTIAAGNVGLEHTVRNALKVCEIAGRPDVPVYAGCPQPLLHPSVDAA
HVHGLDGFGDVGLAPAKRTAEAEHAALAILRLSHEHAGKLLLVALGPLTNLALALTLDPTLPQRVARLVVMGGALTGHGN
ITAAAEFNIGFDPEAAHIVFRGFPQFDVADWEATIAHGLLHRDVEHWLAADSARARFYEDISRKTRLWSEDSRGEYWYAA
DALAMAFALHPEGAQRLEQRPVHIELSGAHTRGMTLVDWNRQGGAPDNANLLLAYDIQQFYRLVGAALAAN

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=303, Percent_Identity=33.6633663366337, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788486, Length=304, Percent_Identity=31.5789473684211, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1786213, Length=307, Percent_Identity=33.5504885993485, Blast_Score=117, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI32565474, Length=290, Percent_Identity=31.0344827586207, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17565698, Length=294, Percent_Identity=30.952380952381, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320608, Length=208, Percent_Identity=32.6923076923077, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24641839, Length=297, Percent_Identity=33.6700336700337, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24583915, Length=306, Percent_Identity=31.3725490196078, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24641837, Length=323, Percent_Identity=30.3405572755418, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33652; Mature: 33520

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKIPLLIDTDPGVDDALALLMAFNDNRHEVVGLTIAAGNVGLEHTVRNALKVCEIAGR
CCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCC
PDVPVYAGCPQPLLHPSVDAAHVHGLDGFGDVGLAPAKRTAEAEHAALAILRLSHEHAGK
CCCCEECCCCCCCCCCCCCHHEECCCCCCCCCCCCCCCHHCHHHHHHEEEEEECCCCCCC
LLLVALGPLTNLALALTLDPTLPQRVARLVVMGGALTGHGNITAAAEFNIGFDPEAAHIV
EEEEEECCCCCEEEEEEECCCHHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCCCEEEE
FRGFPQFDVADWEATIAHGLLHRDVEHWLAADSARARFYEDISRKTRLWSEDSRGEYWYA
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEH
ADALAMAFALHPEGAQRLEQRPVHIELSGAHTRGMTLVDWNRQGGAPDNANLLLAYDIQQ
HHHHHHHHEECCCHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEEECHHH
FYRLVGAALAAN
HHHHHHHHHHCC
>Mature Secondary Structure 
TKKIPLLIDTDPGVDDALALLMAFNDNRHEVVGLTIAAGNVGLEHTVRNALKVCEIAGR
CCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCC
PDVPVYAGCPQPLLHPSVDAAHVHGLDGFGDVGLAPAKRTAEAEHAALAILRLSHEHAGK
CCCCEECCCCCCCCCCCCCHHEECCCCCCCCCCCCCCCHHCHHHHHHEEEEEECCCCCCC
LLLVALGPLTNLALALTLDPTLPQRVARLVVMGGALTGHGNITAAAEFNIGFDPEAAHIV
EEEEEECCCCCEEEEEEECCCHHHHHHHHHHCCCEEECCCCEEEEEEECCCCCCCCEEEE
FRGFPQFDVADWEATIAHGLLHRDVEHWLAADSARARFYEDISRKTRLWSEDSRGEYWYA
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEH
ADALAMAFALHPEGAQRLEQRPVHIELSGAHTRGMTLVDWNRQGGAPDNANLLLAYDIQQ
HHHHHHHHEECCCHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEEECHHH
FYRLVGAALAAN
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA