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The map label for this gene is pgk

Identifier: 21244072

GI number: 21244072

Start: 3943351

End: 3944526

Strand: Reverse

Name: pgk

Synonym: XAC3347

Alternate gene names: 21244072

Gene position: 3944526-3943351 (Counterclockwise)

Preceding gene: 21244073

Following gene: 21244071

Centisome position: 76.21

GC content: 66.24

Gene sequence:

>1176_bases
ATGTCCATTGTCCGCATGACCGACCTCGATCTCTCCGGCAAGCGCGTGCTGATCCGCCAGGATCTCAACGTGCCGATCGA
CAACGGCCAGATCACCTCCGAACAGCGCATCACCGCCTCGGTGCCCACCATCAAGCTAGCGCTGGAAAAAGGTGCGGCCG
TCATGGTGACCTCGCACCTGGGCCGCCCCAAGGAAGGCAGCTGGACCGAAGAAGATTCGCTGGCGCCGGTTGCCACGCGT
CTGGCCGCATTGCTGGGCGTGGACGTGCCGCTGGTGCGCGACTGGGTCGATGGCGTGGAGGTCGCGCCGGGCCAGGTGGT
GCTGCTGGAAAACTGCCGCATGAATGTCGGCGAGGGCAAGGACGATGAGACGCTGGCGCGCAAGTACGCCGCGTTGTGCG
ACGTGTTCGTGATGGATGCCTTCGGCACCGCGCACCGCGCGCAGGCCTCCACGCATGGCGTGATCCGTTTCGCGCCGGTC
GCCGCAGGCGGCCCGCTGTTGATGGCCGAACTCGACGCCCTGGCCAAGGCGCTGGACAACCCCGCCAAGCCCTTGCTGGC
GATCGTGGCCGGCAGCAAGGTGTCCACCAAGCTGGAGCTGCTGTCCAACCTGGTCAACAAGGTCGACCAGCTGATCGTCG
GCGGCGGCATCGCCAACACCTTCATCGCCGCGGCCGGGCATGCCGTGGGCAAGTCGTTGAACGAGCCGGATTTGATCCCG
ACCGCCAACCGGATCGTGGCCGATGCCAAGGCGCGTGGCGCCGAGATCCCGCTGCCCACCGATGTGGTCGTTGCCAAGCA
GTTCCTGCCCGATGCGCAAGCCAGCGTGAAGTCGTTGGACGCGGTCGACGCGGACGATCTGATCCTGGATATCGGCCCGC
AAACCGCGCAGCGCTACGCCGAACTGATCGCCAGTGCCGGTACCGTGGTGTGGAACGGTCCGGTGGGCGTGTTCGAATTC
GAGTCCTTCAGCCACGGCACCGAAACCCTGGCGCGCGCGATCGCCAGCTCCAAGGCGTTCTCGATCGCTGGCGGTGGCGA
CACCCTGGCCGCAGTGGACAAATACGACATCGCCCAGGACGTCACCTACATTTCCACGGGCGGCGGTGCGTTCCTGGAGT
TTCTGGAAGGCAAGACCTTGCCGGCGGTGGCCGCACTCCAGGCCCGCGGTCAGTGA

Upstream 100 bases:

>100_bases
GCTGAGGCAAAGCGCTCACATGTTTGCACCGGCGCGTCTGCTGGGTGCTTCTGCATGACCATTCCCCACTCCCCCTTCCT
AGACCGAACGAGCTGAGCCC

Downstream 100 bases:

>100_bases
GTGCAGCCACGCTGTTTTTCGATCTGGACGGCACGCTGGTCGATTCTGAGCCGGGCATCGTTGCCAGCATCGTGCATGCC
TTCGACGAACTCGGCCAGCC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSIVRMTDLDLSGKRVLIRQDLNVPIDNGQITSEQRITASVPTIKLALEKGAAVMVTSHLGRPKEGSWTEEDSLAPVATR
LAALLGVDVPLVRDWVDGVEVAPGQVVLLENCRMNVGEGKDDETLARKYAALCDVFVMDAFGTAHRAQASTHGVIRFAPV
AAGGPLLMAELDALAKALDNPAKPLLAIVAGSKVSTKLELLSNLVNKVDQLIVGGGIANTFIAAAGHAVGKSLNEPDLIP
TANRIVADAKARGAEIPLPTDVVVAKQFLPDAQASVKSLDAVDADDLILDIGPQTAQRYAELIASAGTVVWNGPVGVFEF
ESFSHGTETLARAIASSKAFSIAGGGDTLAAVDKYDIAQDVTYISTGGGAFLEFLEGKTLPAVAALQARGQ

Sequences:

>Translated_391_residues
MSIVRMTDLDLSGKRVLIRQDLNVPIDNGQITSEQRITASVPTIKLALEKGAAVMVTSHLGRPKEGSWTEEDSLAPVATR
LAALLGVDVPLVRDWVDGVEVAPGQVVLLENCRMNVGEGKDDETLARKYAALCDVFVMDAFGTAHRAQASTHGVIRFAPV
AAGGPLLMAELDALAKALDNPAKPLLAIVAGSKVSTKLELLSNLVNKVDQLIVGGGIANTFIAAAGHAVGKSLNEPDLIP
TANRIVADAKARGAEIPLPTDVVVAKQFLPDAQASVKSLDAVDADDLILDIGPQTAQRYAELIASAGTVVWNGPVGVFEF
ESFSHGTETLARAIASSKAFSIAGGGDTLAAVDKYDIAQDVTYISTGGGAFLEFLEGKTLPAVAALQARGQ
>Mature_390_residues
SIVRMTDLDLSGKRVLIRQDLNVPIDNGQITSEQRITASVPTIKLALEKGAAVMVTSHLGRPKEGSWTEEDSLAPVATRL
AALLGVDVPLVRDWVDGVEVAPGQVVLLENCRMNVGEGKDDETLARKYAALCDVFVMDAFGTAHRAQASTHGVIRFAPVA
AGGPLLMAELDALAKALDNPAKPLLAIVAGSKVSTKLELLSNLVNKVDQLIVGGGIANTFIAAAGHAVGKSLNEPDLIPT
ANRIVADAKARGAEIPLPTDVVVAKQFLPDAQASVKSLDAVDADDLILDIGPQTAQRYAELIASAGTVVWNGPVGVFEFE
SFSHGTETLARAIASSKAFSIAGGGDTLAAVDKYDIAQDVTYISTGGGAFLEFLEGKTLPAVAALQARGQ

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=43.0656934306569, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI31543397, Length=409, Percent_Identity=42.5427872860636, Blast_Score=294, Evalue=1e-79,
Organism=Escherichia coli, GI1789294, Length=391, Percent_Identity=63.4271099744246, Blast_Score=493, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17508823, Length=406, Percent_Identity=45.320197044335, Blast_Score=307, Evalue=8e-84,
Organism=Saccharomyces cerevisiae, GI6319857, Length=414, Percent_Identity=42.7536231884058, Blast_Score=291, Evalue=2e-79,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=43.3414043583535, Blast_Score=284, Evalue=6e-77,
Organism=Drosophila melanogaster, GI24581203, Length=409, Percent_Identity=40.8312958435208, Blast_Score=265, Evalue=5e-71,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_XANAC (Q8PHB2)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643654.1
- ProteinModelPortal:   Q8PHB2
- SMR:   Q8PHB2
- GeneID:   1157418
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3347
- NMPDR:   fig|190486.1.peg.3298
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- ProtClustDB:   PRK00073
- BioCyc:   XAXO190486:XAC3347-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 40789; Mature: 40658

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 113-113 BINDING 146-146 BINDING 197-197 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVRMTDLDLSGKRVLIRQDLNVPIDNGQITSEQRITASVPTIKLALEKGAAVMVTSHL
CCEEEEECCCCCCCEEEEEECCCCCCCCCEECCCCEEEECCCEEEEEECCCCEEEEEECC
GRPKEGSWTEEDSLAPVATRLAALLGVDVPLVRDWVDGVEVAPGQVVLLENCRMNVGEGK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCEEEEECCCCCCCCCC
DDETLARKYAALCDVFVMDAFGTAHRAQASTHGVIRFAPVAAGGPLLMAELDALAKALDN
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCC
PAKPLLAIVAGSKVSTKLELLSNLVNKVDQLIVGGGIANTFIAAAGHAVGKSLNEPDLIP
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
TANRIVADAKARGAEIPLPTDVVVAKQFLPDAQASVKSLDAVDADDLILDIGPQTAQRYA
CCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHH
ELIASAGTVVWNGPVGVFEFESFSHGTETLARAIASSKAFSIAGGGDTLAAVDKYDIAQD
HHHHHCCCEEECCCCCEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHC
VTYISTGGGAFLEFLEGKTLPAVAALQARGQ
CEEEECCCHHHHHHHCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
SIVRMTDLDLSGKRVLIRQDLNVPIDNGQITSEQRITASVPTIKLALEKGAAVMVTSHL
CEEEEECCCCCCCEEEEEECCCCCCCCCEECCCCEEEECCCEEEEEECCCCEEEEEECC
GRPKEGSWTEEDSLAPVATRLAALLGVDVPLVRDWVDGVEVAPGQVVLLENCRMNVGEGK
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCEECCCCEEEEECCCCCCCCCC
DDETLARKYAALCDVFVMDAFGTAHRAQASTHGVIRFAPVAAGGPLLMAELDALAKALDN
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCC
PAKPLLAIVAGSKVSTKLELLSNLVNKVDQLIVGGGIANTFIAAAGHAVGKSLNEPDLIP
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
TANRIVADAKARGAEIPLPTDVVVAKQFLPDAQASVKSLDAVDADDLILDIGPQTAQRYA
CCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHH
ELIASAGTVVWNGPVGVFEFESFSHGTETLARAIASSKAFSIAGGGDTLAAVDKYDIAQD
HHHHHCCCEEECCCCCEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHC
VTYISTGGGAFLEFLEGKTLPAVAALQARGQ
CEEEECCCHHHHHHHCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217