Click here to switch to the map view.

The map label for this gene is cysD

Identifier: 21244054

GI number: 21244054

Start: 3921818

End: 3922726

Strand: Reverse

Name: cysD

Synonym: XAC3329

Alternate gene names: 21244054

Gene position: 3922726-3921818 (Counterclockwise)

Preceding gene: 21244058

Following gene: 21244053

Centisome position: 75.79

GC content: 66.45

Gene sequence:

>909_bases
ATGACTCTGCCGCCGCTGTCCCATCTCGACCGGCTCGAAGCCGAAAGCATCCACATCCTGCGCGAAGTCGCCGCCGAGTT
CCGGGTTCCGGTCATGCTGTATTCGGTGGGCAAGGACAGCTCGGTGCTGCTGCACCTGCTGCTCAAGGCGTTCGCGCCGT
CGCGGCCGCCGATCCCGCTGCTGCATATCGACACACGCTGGAAGTTCCGCGAGATGATCGCCTTCCGCGACCGCCGCGCG
GCCGAGACCGGGGTGGACCTGCGCGTGCACATCAACCCCGACGGCGTTATGCAGGACGTTGGCCCGATCAGCCACGGTGC
CGCCGTGCACACCGACATCATGAAAACCCAGGGCCTGAAGCAGGCCCTGGAGCACGGCGGCTTCGATGCGGCGATCGGCG
GGGCACGCCGCGACGAAGAGAAATCGCGCGCCAAGGAGCGCGTCTTTTCGTTTCGCACTGCGCGCCACCGCTGGGATCCG
AAGAACCAGCGCCCGGAGTTGTGGAATCTGTACAACGCGCGCACCGGTCCGGGCGAAAGCGTGCGTGTGTTTCCGCTCTC
CAACTGGACCGAGCTGGACATCTGGCTGTACATCTACCGCGAGAAGATTCCGGTGGTGCCGCTGTACTTCGCCGCCCCGC
GCCCGGTGGTGGAGCGCGATGGTGCCTGGATCATGGTCGACGACGCGCGCCTGCCGCTGCACCCGGGCGAAACCCCGCAA
CTGCGCTCGGTGCGCTTCCGCACGCTGGGCTGCTACCCGCTCACCGGCGCCATCGACTCCAACGCCGACACGCTGGAAGC
GGTCATCGCCGAAATGCTGGTCAACACCAGCTCCGAGCGCCAGGGCCGCATGATCGACCACGCCCCGGGCGCCTCGATGG
AACAGAAGAAGCTTGAGGGGTATTTCTGA

Upstream 100 bases:

>100_bases
TATGCCGGGCGCTTATTTCGTGCAGTCATCAGGGCGGCACACACTGCGGCGCCTTGCCCTACCATCGGCTGGTCTGCCCG
CCGACATCCACGATATCCCG

Downstream 100 bases:

>100_bases
TGGGCAGCGAATGGGGAATCGGGAATGGAGAATCGGAACAGCAGATTGCCGCGGATGCAGTGAGGGAGGGCACCACCGTT
GCGATTCCCGATTCCCGATT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTLPPLSHLDRLEAESIHILREVAAEFRVPVMLYSVGKDSSVLLHLLLKAFAPSRPPIPLLHIDTRWKFREMIAFRDRRA
AETGVDLRVHINPDGVMQDVGPISHGAAVHTDIMKTQGLKQALEHGGFDAAIGGARRDEEKSRAKERVFSFRTARHRWDP
KNQRPELWNLYNARTGPGESVRVFPLSNWTELDIWLYIYREKIPVVPLYFAAPRPVVERDGAWIMVDDARLPLHPGETPQ
LRSVRFRTLGCYPLTGAIDSNADTLEAVIAEMLVNTSSERQGRMIDHAPGASMEQKKLEGYF

Sequences:

>Translated_302_residues
MTLPPLSHLDRLEAESIHILREVAAEFRVPVMLYSVGKDSSVLLHLLLKAFAPSRPPIPLLHIDTRWKFREMIAFRDRRA
AETGVDLRVHINPDGVMQDVGPISHGAAVHTDIMKTQGLKQALEHGGFDAAIGGARRDEEKSRAKERVFSFRTARHRWDP
KNQRPELWNLYNARTGPGESVRVFPLSNWTELDIWLYIYREKIPVVPLYFAAPRPVVERDGAWIMVDDARLPLHPGETPQ
LRSVRFRTLGCYPLTGAIDSNADTLEAVIAEMLVNTSSERQGRMIDHAPGASMEQKKLEGYF
>Mature_301_residues
TLPPLSHLDRLEAESIHILREVAAEFRVPVMLYSVGKDSSVLLHLLLKAFAPSRPPIPLLHIDTRWKFREMIAFRDRRAA
ETGVDLRVHINPDGVMQDVGPISHGAAVHTDIMKTQGLKQALEHGGFDAAIGGARRDEEKSRAKERVFSFRTARHRWDPK
NQRPELWNLYNARTGPGESVRVFPLSNWTELDIWLYIYREKIPVVPLYFAAPRPVVERDGAWIMVDDARLPLHPGETPQL
RSVRFRTLGCYPLTGAIDSNADTLEAVIAEMLVNTSSERQGRMIDHAPGASMEQKKLEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=67.3400673400673, Blast_Score=417, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_XANAC (Q8PHD0)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643636.1
- HSSP:   Q87WW0
- ProteinModelPortal:   Q8PHD0
- SMR:   Q8PHD0
- GeneID:   1157400
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3329
- NMPDR:   fig|190486.1.peg.3280
- HOGENOM:   HBG758022
- OMA:   HYGFDAA
- ProtClustDB:   PRK05253
- BioCyc:   XAXO190486:XAC3329-MONOMER
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 34242; Mature: 34111

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPPLSHLDRLEAESIHILREVAAEFRVPVMLYSVGKDSSVLLHLLLKAFAPSRPPIPL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEE
LHIDTRWKFREMIAFRDRRAAETGVDLRVHINPDGVMQDVGPISHGAAVHTDIMKTQGLK
EEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHH
QALEHGGFDAAIGGARRDEEKSRAKERVFSFRTARHRWDPKNQRPELWNLYNARTGPGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
VRVFPLSNWTELDIWLYIYREKIPVVPLYFAAPRPVVERDGAWIMVDDARLPLHPGETPQ
EEEEECCCCCEEEEEEEEEECCCCEEEEEECCCCCHHCCCCCEEEEECCCCCCCCCCCCC
LRSVRFRTLGCYPLTGAIDSNADTLEAVIAEMLVNTSSERQGRMIDHAPGASMEQKKLEG
HHHEEEEEECCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHCC
YF
CC
>Mature Secondary Structure 
TLPPLSHLDRLEAESIHILREVAAEFRVPVMLYSVGKDSSVLLHLLLKAFAPSRPPIPL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEE
LHIDTRWKFREMIAFRDRRAAETGVDLRVHINPDGVMQDVGPISHGAAVHTDIMKTQGLK
EEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHH
QALEHGGFDAAIGGARRDEEKSRAKERVFSFRTARHRWDPKNQRPELWNLYNARTGPGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCC
VRVFPLSNWTELDIWLYIYREKIPVVPLYFAAPRPVVERDGAWIMVDDARLPLHPGETPQ
EEEEECCCCCEEEEEEEEEECCCCEEEEEECCCCCHHCCCCCEEEEECCCCCCCCCCCCC
LRSVRFRTLGCYPLTGAIDSNADTLEAVIAEMLVNTSSERQGRMIDHAPGASMEQKKLEG
HHHEEEEEECCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHCC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217