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The map label for this gene is metH [H]

Identifier: 21242311

GI number: 21242311

Start: 1803286

End: 1804377

Strand: Reverse

Name: metH [H]

Synonym: XAC1560

Alternate gene names: 21242311

Gene position: 1804377-1803286 (Counterclockwise)

Preceding gene: 21242312

Following gene: 21242310

Centisome position: 34.86

GC content: 64.74

Gene sequence:

>1092_bases
TTGCCGTGGCTGCATCCAGAACGCGCAGCCAAGCTCACTGCCGCGCTTGCCGAGCGCATCCTCATCATCGATGGGGCGAT
GGGCACCATGATCCAGCGCCACGATCTGCAGGAGCCCGATTACCGCGGCACCCGCTTTGCCGACGGCTACGACAGTGCGC
ACGTGCACGGCCCCGGTTGCGACCACGCGCACGTACCGCAGGGCCACGACCTGAAGGGCAACAACGATCTGCTGCTGTTG
ACCCGCCCGGAAATCATTGCCGGCATTCATCGTGCCTACCTGGAGGCAGGTGCCGATCTCCTGGAAACCAATACCTTCAA
CGCCACGTCGGTGAGCCAGGCCGACTATCACCTGGAACATCTGGTGTACGAGTTGAACAAGGCCGGCGCACAGGTCGCGC
GCGCCTGTTGCGACGACGTCGAGGCGTTGACCCCGCACAAGCCGCGCTTTGTGATCGGCGTGCTCGGCCCCACCAGCCGT
ACTGCCTCGATCAGCCCGGACGTCAACGACCCGGGCTACCGCAACACCAGTTTCGATGCGCTGCGCGCAACCTATCGCGA
GGCTATCGAGGGCCTGATCGATGGCGGCGCGGATACGCTGATGGTGGAAACCATCTTCGACACGCTCAACGCCAAGGCCG
CGCTGTATGCGATCGAAGAGGTCTTCGACGCGCGCGGCGGGCGTTTGCCGGTGATGGTCTCCGGCACCATCACCGATGCA
TCCGGTCGCACCTTGTCGGGACAGACTGCCGAAGCGTTTTATGCATCGGTCGCGCACGGCCGGCCGTTGTCGATCGGCCT
GAATTGCGCGCTCGGCGCCAGGGATTTGCGCCCGCATGTGGAAACGCTGGCGCAGATCGCCGACACCTATGTCAGTGCGC
ATCCGAATGCCGGCTTGCCGAATGCCTTCGGCGAATACGACGAGACGCCGGAAGAAATGGCGCAGACGCTGCGCGAATTC
GCACAGGCCGGCCTGCTCAATCTGGTCGGTGGCTGCTGCGGCACCTCGCCCGACCATATCCGCGCCATTGCCGAGGCGGT
CGCTGATCTGCCACCGCGGCAATTGCCGGGCGCACAAGAGCTGGCGGCGTGA

Upstream 100 bases:

>100_bases
GGGATTCGGGATTAGTGAAAAACAAAGACACCGCTGCGCGGAGAACCGGACCATGACGCACGTGCGAATCCCTAATCCCG
AATCTCCAATCCGGTTTGCA

Downstream 100 bases:

>100_bases
TGCGTCAGGCTCACCCTCATCCGCCCTTCGGGCACCTTCTCCCGCTAGCGGGAGAAGGACAGCGCGGCGCTTGGATTGCC
GCAAGGACGCGCCGTGCGCA

Product: 5-methyltetrahydrofolate-homocysteine methyl transferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MPWLHPERAAKLTAALAERILIIDGAMGTMIQRHDLQEPDYRGTRFADGYDSAHVHGPGCDHAHVPQGHDLKGNNDLLLL
TRPEIIAGIHRAYLEAGADLLETNTFNATSVSQADYHLEHLVYELNKAGAQVARACCDDVEALTPHKPRFVIGVLGPTSR
TASISPDVNDPGYRNTSFDALRATYREAIEGLIDGGADTLMVETIFDTLNAKAALYAIEEVFDARGGRLPVMVSGTITDA
SGRTLSGQTAEAFYASVAHGRPLSIGLNCALGARDLRPHVETLAQIADTYVSAHPNAGLPNAFGEYDETPEEMAQTLREF
AQAGLLNLVGGCCGTSPDHIRAIAEAVADLPPRQLPGAQELAA

Sequences:

>Translated_363_residues
MPWLHPERAAKLTAALAERILIIDGAMGTMIQRHDLQEPDYRGTRFADGYDSAHVHGPGCDHAHVPQGHDLKGNNDLLLL
TRPEIIAGIHRAYLEAGADLLETNTFNATSVSQADYHLEHLVYELNKAGAQVARACCDDVEALTPHKPRFVIGVLGPTSR
TASISPDVNDPGYRNTSFDALRATYREAIEGLIDGGADTLMVETIFDTLNAKAALYAIEEVFDARGGRLPVMVSGTITDA
SGRTLSGQTAEAFYASVAHGRPLSIGLNCALGARDLRPHVETLAQIADTYVSAHPNAGLPNAFGEYDETPEEMAQTLREF
AQAGLLNLVGGCCGTSPDHIRAIAEAVADLPPRQLPGAQELAA
>Mature_362_residues
PWLHPERAAKLTAALAERILIIDGAMGTMIQRHDLQEPDYRGTRFADGYDSAHVHGPGCDHAHVPQGHDLKGNNDLLLLT
RPEIIAGIHRAYLEAGADLLETNTFNATSVSQADYHLEHLVYELNKAGAQVARACCDDVEALTPHKPRFVIGVLGPTSRT
ASISPDVNDPGYRNTSFDALRATYREAIEGLIDGGADTLMVETIFDTLNAKAALYAIEEVFDARGGRLPVMVSGTITDAS
GRTLSGQTAEAFYASVAHGRPLSIGLNCALGARDLRPHVETLAQIADTYVSAHPNAGLPNAFGEYDETPEEMAQTLREFA
QAGLLNLVGGCCGTSPDHIRAIAEAVADLPPRQLPGAQELAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=346, Percent_Identity=53.1791907514451, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1790450, Length=349, Percent_Identity=59.3123209169054, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1786456, Length=335, Percent_Identity=25.6716417910448, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535405, Length=349, Percent_Identity=53.5816618911175, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38818; Mature: 38687

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWLHPERAAKLTAALAERILIIDGAMGTMIQRHDLQEPDYRGTRFADGYDSAHVHGPGC
CCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCC
DHAHVPQGHDLKGNNDLLLLTRPEIIAGIHRAYLEAGADLLETNTFNATSVSQADYHLEH
CCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHH
LVYELNKAGAQVARACCDDVEALTPHKPRFVIGVLGPTSRTASISPDVNDPGYRNTSFDA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
LRATYREAIEGLIDGGADTLMVETIFDTLNAKAALYAIEEVFDARGGRLPVMVSGTITDA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEEEECC
SGRTLSGQTAEAFYASVAHGRPLSIGLNCALGARDLRPHVETLAQIADTYVSAHPNAGLP
CCCEECCCHHHHHHHHHHCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
NAFGEYDETPEEMAQTLREFAQAGLLNLVGGCCGTSPDHIRAIAEAVADLPPRQLPGAQE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHH
LAA
HCC
>Mature Secondary Structure 
PWLHPERAAKLTAALAERILIIDGAMGTMIQRHDLQEPDYRGTRFADGYDSAHVHGPGC
CCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCC
DHAHVPQGHDLKGNNDLLLLTRPEIIAGIHRAYLEAGADLLETNTFNATSVSQADYHLEH
CCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHH
LVYELNKAGAQVARACCDDVEALTPHKPRFVIGVLGPTSRTASISPDVNDPGYRNTSFDA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
LRATYREAIEGLIDGGADTLMVETIFDTLNAKAALYAIEEVFDARGGRLPVMVSGTITDA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEEEECC
SGRTLSGQTAEAFYASVAHGRPLSIGLNCALGARDLRPHVETLAQIADTYVSAHPNAGLP
CCCEECCCHHHHHHHHHHCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
NAFGEYDETPEEMAQTLREFAQAGLLNLVGGCCGTSPDHIRAIAEAVADLPPRQLPGAQE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHH
LAA
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]