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The map label for this gene is purB [H]

Identifier: 21242290

GI number: 21242290

Start: 1779734

End: 1781101

Strand: Reverse

Name: purB [H]

Synonym: XAC1539

Alternate gene names: 21242290

Gene position: 1781101-1779734 (Counterclockwise)

Preceding gene: 21242292

Following gene: 77748593

Centisome position: 34.41

GC content: 65.72

Gene sequence:

>1368_bases
ATGTCGGATTCCGCTCTGCTCGCCCTGTCCCCGCTCGACGGCCGCTACGCCTCCAAAGTGGATGCGCTGCGCCCGATCTT
CTCCGAATACGGCCTGATCAAGGCGCGGGTCAAGGTCGAAATCGAATGGCTGCTGGCACTGGCCGCCGAGCCGGGCATCG
CCGAACTGGCGCCGTTCTCGCCGGCTGCCACGCAGCGGCTGCGCGCCCTGGCCGACGATTTCGGCGTGGCCCATGCCGCG
CGGGTCAAGGAAATCGAGCGCACCACCAACCATGACGTCAAGGCGGTGGAGTACTTCATCAAGGAGCAGCTCAAGGACGA
TGCCGAACTCGGCCCGGCGCTGGAATTCGTGCACTTTGCCTGCACCAGCGAAGACATCAACAACCTGAGCTACGGGCTGA
TGCTGGAGCAGGCACGCCGTCAGGTGTTGCTGCCCAGCCTGGATGGTATTACCGCCGCGCTGCGCACCCTCGCCCACGCC
CAGGCCGCCCAGCCGATGCTCTCGCGCACCCACGGCCAGACCGCCTCGCCCACCACGCTGGGCAAGGAAATCGCCAACGT
GGTCGCGCGCCTGGAGCGCCAGCGCAAGCAGATCGCCGCGGTCGAACTGACCGGCAAGATCAACGGCGCGGTCGGTAATT
ACAACGCGCACCTGGTGTCGTACCCGGACCTGGACTGGTCGGCGTTCGCACAGCGTTTCGTGGAAAGCCTGGGCCTGGTG
TTCAACCCCTACACCACCCAGATCGAGCCGCACGACAATGTGGCCGAAATCGGCGATGCCAGCCGCCGCGCCAACACCAT
CCTGATCGACCTGGCGCGCGACATCTGGGGCTATATCTCGCTGGGCTATTTCAAGCAGAAGCTGAAAGAGGGCGAAGTTG
GCTCCTCGACCATGCCGCATAAGGTCAACCCCATCGACTTCGAAAATGCCGAGGGCAATTTCGGCATCGCCAACGCGCTG
TTCGAACATTTCTCCGCCAAGTTGCCGATCAGCCGCTGGCAGCGCGACCTCACCGATTCCACCGTGCTGCGCGCACTCGG
CACCGCGTTCGGCCATACCCAGGTCGCGCTGGATTCGCTGGCCAAGGGCCTGGGCAAGCTCACCGTCAACCCCGAGCGGC
TGGATGCCGACCTGGATGCGGCCTGGGAAGTGCTGGCCGAAGCCGTGCAGACGGTCATGCGCCGCCACGGCCTGCCGAAC
CCCTACGAACAACTCAAGGCGCTGACCCGCGGCCAGGGCATCACCGCCGCCTCGATGCAGGCCTTCGTGGAAAGTCTGCA
ACTGCCGGAACAAGACAAGCAGCGCCTGCGCGCGCTCACCCCGGGCGCCTACACCGGGCTGGCCGAGCAGCTGGCGCGCG
CGATCTGA

Upstream 100 bases:

>100_bases
TGCCGTGGGCTTGGGAACCCGTTTTGCAGGGCACCGGGCCTGCCCGCAGCCTCGGCGGCGTAGAATGCTGGCCCCACCGT
CACCGCTGCCTCTCCTGCCC

Downstream 100 bases:

>100_bases
GCTGACCGAACCGCGAGCTTCGGTGGGAGCTTGCGAAGCGGGCCACGTCATCGACCTCATCGGCGAGGCGTCATCGGCAT
CAGCGCATGCCGATGACGCA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSDSALLALSPLDGRYASKVDALRPIFSEYGLIKARVKVEIEWLLALAAEPGIAELAPFSPAATQRLRALADDFGVAHAA
RVKEIERTTNHDVKAVEYFIKEQLKDDAELGPALEFVHFACTSEDINNLSYGLMLEQARRQVLLPSLDGITAALRTLAHA
QAAQPMLSRTHGQTASPTTLGKEIANVVARLERQRKQIAAVELTGKINGAVGNYNAHLVSYPDLDWSAFAQRFVESLGLV
FNPYTTQIEPHDNVAEIGDASRRANTILIDLARDIWGYISLGYFKQKLKEGEVGSSTMPHKVNPIDFENAEGNFGIANAL
FEHFSAKLPISRWQRDLTDSTVLRALGTAFGHTQVALDSLAKGLGKLTVNPERLDADLDAAWEVLAEAVQTVMRRHGLPN
PYEQLKALTRGQGITAASMQAFVESLQLPEQDKQRLRALTPGAYTGLAEQLARAI

Sequences:

>Translated_455_residues
MSDSALLALSPLDGRYASKVDALRPIFSEYGLIKARVKVEIEWLLALAAEPGIAELAPFSPAATQRLRALADDFGVAHAA
RVKEIERTTNHDVKAVEYFIKEQLKDDAELGPALEFVHFACTSEDINNLSYGLMLEQARRQVLLPSLDGITAALRTLAHA
QAAQPMLSRTHGQTASPTTLGKEIANVVARLERQRKQIAAVELTGKINGAVGNYNAHLVSYPDLDWSAFAQRFVESLGLV
FNPYTTQIEPHDNVAEIGDASRRANTILIDLARDIWGYISLGYFKQKLKEGEVGSSTMPHKVNPIDFENAEGNFGIANAL
FEHFSAKLPISRWQRDLTDSTVLRALGTAFGHTQVALDSLAKGLGKLTVNPERLDADLDAAWEVLAEAVQTVMRRHGLPN
PYEQLKALTRGQGITAASMQAFVESLQLPEQDKQRLRALTPGAYTGLAEQLARAI
>Mature_454_residues
SDSALLALSPLDGRYASKVDALRPIFSEYGLIKARVKVEIEWLLALAAEPGIAELAPFSPAATQRLRALADDFGVAHAAR
VKEIERTTNHDVKAVEYFIKEQLKDDAELGPALEFVHFACTSEDINNLSYGLMLEQARRQVLLPSLDGITAALRTLAHAQ
AAQPMLSRTHGQTASPTTLGKEIANVVARLERQRKQIAAVELTGKINGAVGNYNAHLVSYPDLDWSAFAQRFVESLGLVF
NPYTTQIEPHDNVAEIGDASRRANTILIDLARDIWGYISLGYFKQKLKEGEVGSSTMPHKVNPIDFENAEGNFGIANALF
EHFSAKLPISRWQRDLTDSTVLRALGTAFGHTQVALDSLAKGLGKLTVNPERLDADLDAAWEVLAEAVQTVMRRHGLPNP
YEQLKALTRGQGITAASMQAFVESLQLPEQDKQRLRALTPGAYTGLAEQLARAI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=448, Percent_Identity=58.0357142857143, Blast_Score=549, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49812; Mature: 49681

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSALLALSPLDGRYASKVDALRPIFSEYGLIKARVKVEIEWLLALAAEPGIAELAPFS
CCCCCEEEECCCCCHHHHHHHHHHHHHHHCCEEEEHHEEEHHHHHHHHCCCCHHHCCCCC
PAATQRLRALADDFGVAHAARVKEIERTTNHDVKAVEYFIKEQLKDDAELGPALEFVHFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHCCHHHHHHHHH
CTSEDINNLSYGLMLEQARRQVLLPSLDGITAALRTLAHAQAAQPMLSRTHGQTASPTTL
HCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GKEIANVVARLERQRKQIAAVELTGKINGAVGNYNAHLVSYPDLDWSAFAQRFVESLGLV
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCE
FNPYTTQIEPHDNVAEIGDASRRANTILIDLARDIWGYISLGYFKQKLKEGEVGSSTMPH
ECCCCCCCCCCCCHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KVNPIDFENAEGNFGIANALFEHFSAKLPISRWQRDLTDSTVLRALGTAFGHTQVALDSL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
AKGLGKLTVNPERLDADLDAAWEVLAEAVQTVMRRHGLPNPYEQLKALTRGQGITAASMQ
HHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
AFVESLQLPEQDKQRLRALTPGAYTGLAEQLARAI
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SDSALLALSPLDGRYASKVDALRPIFSEYGLIKARVKVEIEWLLALAAEPGIAELAPFS
CCCCEEEECCCCCHHHHHHHHHHHHHHHCCEEEEHHEEEHHHHHHHHCCCCHHHCCCCC
PAATQRLRALADDFGVAHAARVKEIERTTNHDVKAVEYFIKEQLKDDAELGPALEFVHFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHCCHHHHHHHHH
CTSEDINNLSYGLMLEQARRQVLLPSLDGITAALRTLAHAQAAQPMLSRTHGQTASPTTL
HCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GKEIANVVARLERQRKQIAAVELTGKINGAVGNYNAHLVSYPDLDWSAFAQRFVESLGLV
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCE
FNPYTTQIEPHDNVAEIGDASRRANTILIDLARDIWGYISLGYFKQKLKEGEVGSSTMPH
ECCCCCCCCCCCCHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KVNPIDFENAEGNFGIANALFEHFSAKLPISRWQRDLTDSTVLRALGTAFGHTQVALDSL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
AKGLGKLTVNPERLDADLDAAWEVLAEAVQTVMRRHGLPNPYEQLKALTRGQGITAASMQ
HHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
AFVESLQLPEQDKQRLRALTPGAYTGLAEQLARAI
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]