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The map label for this gene is pleD [H]

Identifier: 21242240

GI number: 21242240

Start: 1720078

End: 1721778

Strand: Reverse

Name: pleD [H]

Synonym: XAC1488

Alternate gene names: 21242240

Gene position: 1721778-1720078 (Counterclockwise)

Preceding gene: 21242242

Following gene: 21242238

Centisome position: 33.27

GC content: 65.78

Gene sequence:

>1701_bases
GTGTTGACCAAAAGAACCAAACGTCGCGCTGCCGGGGCCTTGCTGGTGGGCGGGCTGATCTTTGTCCTGATCGGCGTGGG
CGGGTATCTCACGACGCAGCGTTCGCTGTCGGACGCCGGCTGGGTGACGCACACGCAGGAAGTGATTGCCAGCATCGACG
AGATCCAGGCGGGCATGCTGTCGGCGGAATCGTCGGCGCGCGGTTATGTCTTGACCGACAACGAGGCCTTTCTGGGCGTG
TATGCCGATGCGATCGGCCGCCTGCCCGAGCGCATCGTGCGGCTGGATGCCCTGGTGCAGGACAACCCGACGCAGCGGCG
CAATGTGGTCGTGCTGAGCCGTTTGGTGGACGCGCGCCTGGTGCAGATCGACGAGTTATTGCATAGCTATCGCAAGCAAG
GCCTGGATGGCGCGCGTGCTGCCATCGCGCGCGGGGTGTTCCAGACCTCTTCATCGCTGCGCCGCCAGGTGCAGACCATG
ACGGAGCTGGAGCGGCAGCAACTGATCCGGCGCAACGCAGCCACCACCCGCAGCGCACAGTGGGTGCTGTGGGTGACTGT
GATCGGCATTGCCAGTGGCTTGCTGGTGATGCTGCTGGCCTACCGCTTGTTGTCGCGCGAGCTGGAACGGCGTACGCGTG
CCGAAGACGACGCCAGCCGGACCAGCGAGCGCCTGATCGAGTCGTTTTCCGCACTCGAACGCACCTCCGCTGGCCTGGAG
GCGCTGGCACGCTACTCCGGGTTGCTGCAGAACTGCCGCGATGCCGAAGAAGCGCTGGAAATCACTGCACGCACGATTGC
ACCGCTGATTCCCGGTGCTGCCGGCGCGGTCTACTTGCTACGCGCCTCGCGCGATCGCGCCGAACAGGTGGTCGCCTGGG
GCGAGATGGCAGACGACGCCAGTGCCAGCATCGCGCCGGATAACTGCTGGGCGCTGCGCCGCGACCGCACCCATGTGGTG
ATGGACGCCGGCCAGGGCATGCTGTGCCAGCATGCCGGCGTCAACCTTCCGGCGCATGCGGCCACGGCCTGTATTCCGCT
ATCGGCGCAGGGCACGCAGTTGGGAATGCTGGCATTGCGTAGCGAAGACCCTGACGATCTTGCCTCGCTGACCGTGGCCG
AGGCGGCAGCGGAACAACTCTCCTTGGCGCTGCACAACCTGCGCCTGCGCGAAACGCTGCGCCAGCAATCGATCCGCGAC
CCGCTGACAGGCCTGTACAACCGCCGCTATCTGGAAGAAACCCTCAGTCACGAATTGGCGCGCTGCACGCGCCGCGAGCT
GCCCTTGTCGGTGCTGATGCTGGATGTGGATCACTTCAAGCGGTTCAACGACCTGCACGGGCACAGCGGCGGCGACCGCG
TGCTGGCGGCCATTGGCGAATTGTTGCTGGCGCAGACGCGTGGCGAAGACATCTGCTGCCGGTACGGCGGCGAAGAATTG
ACGATCATCCTGCCCGAAGTCGATCTGCCAACCGCGTGCATGATGGCGGAGAAATTGCGCGCCGGCATCGAGGCCCTGCA
GGTCATGGGCGATGGCGTCTGTCTGCCGAAGGTCACCGCCTCGTTCGGCGCGGCCAGCTTTCCCGCTCACGCCAGCACCG
TGGCCTCGTTGCTGCAACGGGCGGACGCGGCGCTGTATCGCGCCAAACAGGGTGGCCGCAATCAGGTCGTGAGCGCGGAT
GGGCTTGCATCCGTGGCCTGA

Upstream 100 bases:

>100_bases
GGGGCGATGTGCCGTCTGCCAGCTGACTCGGCAAGCGAAGTGGAGCGTAGGCACGCGGCTTGCATCGGCCTCGACCAGAC
AATCGTGCCGGGGGAACGCG

Downstream 100 bases:

>100_bases
ACGCAAGCAGCCGGGCGTGCTGCTGCGGTCTGTCGCAGAGGCCTGTGTCGCGCAGTCGACGCTCAGATCCGCATCGCACG
CGCTTGGCTAACCCGGGTGC

Product: sensor histidine kinase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MLTKRTKRRAAGALLVGGLIFVLIGVGGYLTTQRSLSDAGWVTHTQEVIASIDEIQAGMLSAESSARGYVLTDNEAFLGV
YADAIGRLPERIVRLDALVQDNPTQRRNVVVLSRLVDARLVQIDELLHSYRKQGLDGARAAIARGVFQTSSSLRRQVQTM
TELERQQLIRRNAATTRSAQWVLWVTVIGIASGLLVMLLAYRLLSRELERRTRAEDDASRTSERLIESFSALERTSAGLE
ALARYSGLLQNCRDAEEALEITARTIAPLIPGAAGAVYLLRASRDRAEQVVAWGEMADDASASIAPDNCWALRRDRTHVV
MDAGQGMLCQHAGVNLPAHAATACIPLSAQGTQLGMLALRSEDPDDLASLTVAEAAAEQLSLALHNLRLRETLRQQSIRD
PLTGLYNRRYLEETLSHELARCTRRELPLSVLMLDVDHFKRFNDLHGHSGGDRVLAAIGELLLAQTRGEDICCRYGGEEL
TIILPEVDLPTACMMAEKLRAGIEALQVMGDGVCLPKVTASFGAASFPAHASTVASLLQRADAALYRAKQGGRNQVVSAD
GLASVA

Sequences:

>Translated_566_residues
MLTKRTKRRAAGALLVGGLIFVLIGVGGYLTTQRSLSDAGWVTHTQEVIASIDEIQAGMLSAESSARGYVLTDNEAFLGV
YADAIGRLPERIVRLDALVQDNPTQRRNVVVLSRLVDARLVQIDELLHSYRKQGLDGARAAIARGVFQTSSSLRRQVQTM
TELERQQLIRRNAATTRSAQWVLWVTVIGIASGLLVMLLAYRLLSRELERRTRAEDDASRTSERLIESFSALERTSAGLE
ALARYSGLLQNCRDAEEALEITARTIAPLIPGAAGAVYLLRASRDRAEQVVAWGEMADDASASIAPDNCWALRRDRTHVV
MDAGQGMLCQHAGVNLPAHAATACIPLSAQGTQLGMLALRSEDPDDLASLTVAEAAAEQLSLALHNLRLRETLRQQSIRD
PLTGLYNRRYLEETLSHELARCTRRELPLSVLMLDVDHFKRFNDLHGHSGGDRVLAAIGELLLAQTRGEDICCRYGGEEL
TIILPEVDLPTACMMAEKLRAGIEALQVMGDGVCLPKVTASFGAASFPAHASTVASLLQRADAALYRAKQGGRNQVVSAD
GLASVA
>Mature_566_residues
MLTKRTKRRAAGALLVGGLIFVLIGVGGYLTTQRSLSDAGWVTHTQEVIASIDEIQAGMLSAESSARGYVLTDNEAFLGV
YADAIGRLPERIVRLDALVQDNPTQRRNVVVLSRLVDARLVQIDELLHSYRKQGLDGARAAIARGVFQTSSSLRRQVQTM
TELERQQLIRRNAATTRSAQWVLWVTVIGIASGLLVMLLAYRLLSRELERRTRAEDDASRTSERLIESFSALERTSAGLE
ALARYSGLLQNCRDAEEALEITARTIAPLIPGAAGAVYLLRASRDRAEQVVAWGEMADDASASIAPDNCWALRRDRTHVV
MDAGQGMLCQHAGVNLPAHAATACIPLSAQGTQLGMLALRSEDPDDLASLTVAEAAAEQLSLALHNLRLRETLRQQSIRD
PLTGLYNRRYLEETLSHELARCTRRELPLSVLMLDVDHFKRFNDLHGHSGGDRVLAAIGELLLAQTRGEDICCRYGGEEL
TIILPEVDLPTACMMAEKLRAGIEALQVMGDGVCLPKVTASFGAASFPAHASTVASLLQRADAALYRAKQGGRNQVVSAD
GLASVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=171, Percent_Identity=38.0116959064327, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=38.3720930232558, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=36.8098159509202, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87082007, Length=174, Percent_Identity=36.7816091954023, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=34.5238095238095, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787802, Length=195, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787816, Length=171, Percent_Identity=35.0877192982456, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=30.2469135802469, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=30.5882352941176, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=28.2352941176471, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081977, Length=184, Percent_Identity=29.3478260869565, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=173, Percent_Identity=24.2774566473988, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 61501; Mature: 61501

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRTKRRAAGALLVGGLIFVLIGVGGYLTTQRSLSDAGWVTHTQEVIASIDEIQAGML
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SAESSARGYVLTDNEAFLGVYADAIGRLPERIVRLDALVQDNPTQRRNVVVLSRLVDARL
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VQIDELLHSYRKQGLDGARAAIARGVFQTSSSLRRQVQTMTELERQQLIRRNAATTRSAQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
WVLWVTVIGIASGLLVMLLAYRLLSRELERRTRAEDDASRTSERLIESFSALERTSAGLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALARYSGLLQNCRDAEEALEITARTIAPLIPGAAGAVYLLRASRDRAEQVVAWGEMADDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCHHHHHHHHHHHHHCCCC
SASIAPDNCWALRRDRTHVVMDAGQGMLCQHAGVNLPAHAATACIPLSAQGTQLGMLALR
CCCCCCCCHHHCCCCCCEEEEECCCCCEECCCCCCCCCHHHHHEEECCCCCCCEEEEEEC
SEDPDDLASLTVAEAAAEQLSLALHNLRLRETLRQQSIRDPLTGLYNRRYLEETLSHELA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RCTRRELPLSVLMLDVDHFKRFNDLHGHSGGDRVLAAIGELLLAQTRGEDICCRYGGEEL
HHHHHCCCHHHEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEE
TIILPEVDLPTACMMAEKLRAGIEALQVMGDGVCLPKVTASFGAASFPAHASTVASLLQR
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
ADAALYRAKQGGRNQVVSADGLASVA
HHHHHHHHHCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MLTKRTKRRAAGALLVGGLIFVLIGVGGYLTTQRSLSDAGWVTHTQEVIASIDEIQAGML
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SAESSARGYVLTDNEAFLGVYADAIGRLPERIVRLDALVQDNPTQRRNVVVLSRLVDARL
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VQIDELLHSYRKQGLDGARAAIARGVFQTSSSLRRQVQTMTELERQQLIRRNAATTRSAQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
WVLWVTVIGIASGLLVMLLAYRLLSRELERRTRAEDDASRTSERLIESFSALERTSAGLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALARYSGLLQNCRDAEEALEITARTIAPLIPGAAGAVYLLRASRDRAEQVVAWGEMADDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCHHHHHHHHHHHHHCCCC
SASIAPDNCWALRRDRTHVVMDAGQGMLCQHAGVNLPAHAATACIPLSAQGTQLGMLALR
CCCCCCCCHHHCCCCCCEEEEECCCCCEECCCCCCCCCHHHHHEEECCCCCCCEEEEEEC
SEDPDDLASLTVAEAAAEQLSLALHNLRLRETLRQQSIRDPLTGLYNRRYLEETLSHELA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RCTRRELPLSVLMLDVDHFKRFNDLHGHSGGDRVLAAIGELLLAQTRGEDICCRYGGEEL
HHHHHCCCHHHEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEE
TIILPEVDLPTACMMAEKLRAGIEALQVMGDGVCLPKVTASFGAASFPAHASTVASLLQR
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHH
ADAALYRAKQGGRNQVVSADGLASVA
HHHHHHHHHCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA