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The map label for this gene is pldA [C]

Identifier: 21242215

GI number: 21242215

Start: 1690479

End: 1691696

Strand: Reverse

Name: pldA [C]

Synonym: XAC1463

Alternate gene names: 21242215

Gene position: 1691696-1690479 (Counterclockwise)

Preceding gene: 21242216

Following gene: 21242211

Centisome position: 32.69

GC content: 62.81

Gene sequence:

>1218_bases
GTGTACACACTGCGTATGCAGACGCGACCATTAAGCTGCGCATCTCTGCGCCTGCCTGGCGCCTTCACTTCGGCCAGACT
GCCCATGTCCAAGCACCACACCCGCCCCCTGATGCTGATCGCACTGGCCGCCGCGCCATTGGCATATGCGCAGGAAATCG
CACCGCAGCCGGCCTCGCCGCAAGCCTGCACCTCGGTGACCTCCGATGCCGCTCGTCTGGCCTGTTACGACAAGGCGCTG
GGCTATACGCCACAGCAAACCGCAGAGGCAGATGCCGCAGCGCAATTGGCCAAGCAGAAGGAGGCCAAGCCCGACGATAC
GCGCGCGATCAGCCGGGTCGGCGATTTCTTCCGCGCCGACGGCCAGGACCAACCGAAAGAAGAAGCCGTGGCCAATGCCG
GCCGCGGCTCGTTGCTGGACCGACGCTGGGAACTGGCCAAGGACTCCAAGCTGGGCACTTTCCAGCTACGCGGTTACAAG
CCGGTGTACCTGTTGCCGGCGTTCTGGACCAGCGACACCAACCGCACCCCGCAGTCGCCGAATCCGGCCAACTCGGTCAA
TACCCCGCAGCAGCTGGACAGCACCGAGCTCAAGTTCCAGCTCAGCTTCAAGACCAAGGTGGTGGAAGACCTGTTCGGCG
ACAACGGCGATATCTGGATGGGCTACACCCAGAGCTCGCGCTGGCAGGCCTACAACACCGATGCGTCGCGCCCGTTCCGC
GAGACCAATTACGAGCCCGAAGCGATGCTGCTCTTTCGCAACAACTACAGCATCTTCGGCTGGAAGGGCCGCATGAGCGG
CATCAGCCTCAATCACATGTCCAACGGGCGCGCCGACCCGCTGTCGCGCTCGTGGAATCGGGTGATCCTGAACTTCGGGC
TGGATCGGGAGAACTGGGCGCTGACACTGCGCCCGTGGTTCCGCATCAAGGAAGACCGCGCCGACGACAACAATCCCGAT
ATCGAGGATTACATGGGCCGCGGCGATGCAACGCTGGTGTACAACAAGGACGGCCACGAATTCGCGCTGATCGCGCGCCA
TTCGCTGCGTGGCGGCGACCGCTCGCACGGTTCGGTGCAGCTGGATTACGGCTTCCCGATCACCAACCTGTTGCGCGGCC
ATGTGCAGGTGTTCGATGGCTACGGCGAAAGCATGATCGACTACAACCACAAGGCAACCTACATCGGTGTGGGCGTGTCG
TTGCTGGAGTGGTTCTAA

Upstream 100 bases:

>100_bases
GCTGACGCGTGGCGGGGCAGACCATCCACATGCCCGCGTTAGGGCGGCACGTTGCAAGCAAACGCCGAGTTGAGGTGATT
CGGTCGAATGTTATGCCGCC

Downstream 100 bases:

>100_bases
GCACTCCCAGCACCGGCTGCGCTCACTGCCACGCGGGCGCAGCCGGTGTCGGTGTTCGGTGTGTACTACTTCAACTTTGC
GAGCCCGATGCGCCCGTGCC

Product: phospholipase

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MYTLRMQTRPLSCASLRLPGAFTSARLPMSKHHTRPLMLIALAAAPLAYAQEIAPQPASPQACTSVTSDAARLACYDKAL
GYTPQQTAEADAAAQLAKQKEAKPDDTRAISRVGDFFRADGQDQPKEEAVANAGRGSLLDRRWELAKDSKLGTFQLRGYK
PVYLLPAFWTSDTNRTPQSPNPANSVNTPQQLDSTELKFQLSFKTKVVEDLFGDNGDIWMGYTQSSRWQAYNTDASRPFR
ETNYEPEAMLLFRNNYSIFGWKGRMSGISLNHMSNGRADPLSRSWNRVILNFGLDRENWALTLRPWFRIKEDRADDNNPD
IEDYMGRGDATLVYNKDGHEFALIARHSLRGGDRSHGSVQLDYGFPITNLLRGHVQVFDGYGESMIDYNHKATYIGVGVS
LLEWF

Sequences:

>Translated_405_residues
MYTLRMQTRPLSCASLRLPGAFTSARLPMSKHHTRPLMLIALAAAPLAYAQEIAPQPASPQACTSVTSDAARLACYDKAL
GYTPQQTAEADAAAQLAKQKEAKPDDTRAISRVGDFFRADGQDQPKEEAVANAGRGSLLDRRWELAKDSKLGTFQLRGYK
PVYLLPAFWTSDTNRTPQSPNPANSVNTPQQLDSTELKFQLSFKTKVVEDLFGDNGDIWMGYTQSSRWQAYNTDASRPFR
ETNYEPEAMLLFRNNYSIFGWKGRMSGISLNHMSNGRADPLSRSWNRVILNFGLDRENWALTLRPWFRIKEDRADDNNPD
IEDYMGRGDATLVYNKDGHEFALIARHSLRGGDRSHGSVQLDYGFPITNLLRGHVQVFDGYGESMIDYNHKATYIGVGVS
LLEWF
>Mature_405_residues
MYTLRMQTRPLSCASLRLPGAFTSARLPMSKHHTRPLMLIALAAAPLAYAQEIAPQPASPQACTSVTSDAARLACYDKAL
GYTPQQTAEADAAAQLAKQKEAKPDDTRAISRVGDFFRADGQDQPKEEAVANAGRGSLLDRRWELAKDSKLGTFQLRGYK
PVYLLPAFWTSDTNRTPQSPNPANSVNTPQQLDSTELKFQLSFKTKVVEDLFGDNGDIWMGYTQSSRWQAYNTDASRPFR
ETNYEPEAMLLFRNNYSIFGWKGRMSGISLNHMSNGRADPLSRSWNRVILNFGLDRENWALTLRPWFRIKEDRADDNNPD
IEDYMGRGDATLVYNKDGHEFALIARHSLRGGDRSHGSVQLDYGFPITNLLRGHVQVFDGYGESMIDYNHKATYIGVGVS
LLEWF

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=280, Percent_Identity=35.7142857142857, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 45536; Mature: 45536

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTLRMQTRPLSCASLRLPGAFTSARLPMSKHHTRPLMLIALAAAPLAYAQEIAPQPASP
CEEEEECCCCCCEEEEECCCCCCCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCC
QACTSVTSDAARLACYDKALGYTPQQTAEADAAAQLAKQKEAKPDDTRAISRVGDFFRAD
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
GQDQPKEEAVANAGRGSLLDRRWELAKDSKLGTFQLRGYKPVYLLPAFWTSDTNRTPQSP
CCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCC
NPANSVNTPQQLDSTELKFQLSFKTKVVEDLFGDNGDIWMGYTQSSRWQAYNTDASRPFR
CCCCCCCCCHHCCCCEEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHH
ETNYEPEAMLLFRNNYSIFGWKGRMSGISLNHMSNGRADPLSRSWNRVILNFGLDRENWA
HCCCCCCEEEEEECCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCE
LTLRPWFRIKEDRADDNNPDIEDYMGRGDATLVYNKDGHEFALIARHSLRGGDRSHGSVQ
EEEECCEEEECCCCCCCCCCHHHHCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCEEE
LDYGFPITNLLRGHVQVFDGYGESMIDYNHKATYIGVGVSLLEWF
EEECCCHHHHHHHHHEEECCCCCHHHCCCCCEEEEECCHHHHHCC
>Mature Secondary Structure
MYTLRMQTRPLSCASLRLPGAFTSARLPMSKHHTRPLMLIALAAAPLAYAQEIAPQPASP
CEEEEECCCCCCEEEEECCCCCCCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCC
QACTSVTSDAARLACYDKALGYTPQQTAEADAAAQLAKQKEAKPDDTRAISRVGDFFRAD
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
GQDQPKEEAVANAGRGSLLDRRWELAKDSKLGTFQLRGYKPVYLLPAFWTSDTNRTPQSP
CCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCC
NPANSVNTPQQLDSTELKFQLSFKTKVVEDLFGDNGDIWMGYTQSSRWQAYNTDASRPFR
CCCCCCCCCHHCCCCEEEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHH
ETNYEPEAMLLFRNNYSIFGWKGRMSGISLNHMSNGRADPLSRSWNRVILNFGLDRENWA
HCCCCCCEEEEEECCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCE
LTLRPWFRIKEDRADDNNPDIEDYMGRGDATLVYNKDGHEFALIARHSLRGGDRSHGSVQ
EEEECCEEEECCCCCCCCCCHHHHCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCEEE
LDYGFPITNLLRGHVQVFDGYGESMIDYNHKATYIGVGVSLLEWF
EEECCCHHHHHHHHHEEECCCCCHHHCCCCCEEEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]