Click here to switch to the map view.

The map label for this gene is ddl [H]

Identifier: 21241551

GI number: 21241551

Start: 926577

End: 927533

Strand: Direct

Name: ddl [H]

Synonym: XAC0781

Alternate gene names: 21241551

Gene position: 926577-927533 (Clockwise)

Preceding gene: 21241550

Following gene: 21241552

Centisome position: 17.9

GC content: 66.77

Gene sequence:

>957_bases
ATGAGCGCGTTGGTCGGCAACGCCCGCACCAGCGATCCGGCTGCCTTTGGCCGCGTCGCCGTACTGCTCGGCGGCACCTC
GTCCGAGCGCGAGGTGTCGTTGAATTCCGGCGGCAACGTGCTGGACGCGCTGCGCGCACGCGGCGTGGATGCGCAGCCGG
TTGATGGCATTCCGGCCTTGGCCAAGGCATTGGTCGCGCAGCAGTTCGATCGCGTGTTCAACGTGCTGCACGGACATAAC
GGCGGCGGCGAAGACGGCATCGTGCAGGGCCTGATGGAAGCCTTCGGCGTGCCATATACCGGCTCGGATGTGCTGGGTTC
GGCGCTGAGCATGGACAAAATCCGCACCAAGCAGGTGTGGTTGTCGCTGGGCCTGTCGACCCCGCGCTATGCGCGGCTGG
CCGCTGGCGCGAGCGCCGAGGACATTCATGCCGCTGCACGCCAGATCGGGTTGCCGATCATCGTCAAGCCGGCCAATGAA
GGTTCCAGCGTCGGCGTCAGCCGCGTGTTCGACCAGGCCCAGCTCGAAGAAGCGGTGACCCTGGCGGCGCGCTACGACGG
CGCGCTGCTGATGGAGCAACTGATCGAAGGCGACGAACTCACCGTGGCCGTGCTCGGCGACACCGCGTTGCCGTCGATCC
GCATCGTGCCCAAGGGCCAGTGGTACGACTACAACGCCAAGTACATCGCCGACGACACGCAATACCTGTGCCCGGGGCTG
GAAGGCGAGGCCGAAGCGCAGATCCGCCAACTCGCGCTGGACGCCTTCCGCGCTGCCGGCTGCCGTGGCTGGGGCCGCGT
GGACGTGATGCGTGATGGCAGCAGTGGCCAGCTGTATCTGCTCGAAGTCAACACCGCTCCCGGCATGACCAGCCACTCGC
TGGTGCCCAAGGCCGCCCGCCAGCTCGGCATCGATTTCGAAACGCTGGTCTGGCGCGTGCTGGAGCAGACGCTATGA

Upstream 100 bases:

>100_bases
ACGTCCTGCAGGACGGCGATCTGCTGTTGATGATGGGCGCCGGCGATATCGGTTATGTGGCCCAGCACATCATCAACAAC
GGGTTTGTCGGAGAACCGGC

Downstream 100 bases:

>100_bases
AGCCGGGAATCGTGAATCGTGAATCGGGAATGGGTAACAGCGGATGCGCTGTTGCCGTTGCGATTCCCTATTCCCGACTC
CCAATTCCCGTGTGCGGAGC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase B; D-alanylalanine synthetase B [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSALVGNARTSDPAAFGRVAVLLGGTSSEREVSLNSGGNVLDALRARGVDAQPVDGIPALAKALVAQQFDRVFNVLHGHN
GGGEDGIVQGLMEAFGVPYTGSDVLGSALSMDKIRTKQVWLSLGLSTPRYARLAAGASAEDIHAAARQIGLPIIVKPANE
GSSVGVSRVFDQAQLEEAVTLAARYDGALLMEQLIEGDELTVAVLGDTALPSIRIVPKGQWYDYNAKYIADDTQYLCPGL
EGEAEAQIRQLALDAFRAAGCRGWGRVDVMRDGSSGQLYLLEVNTAPGMTSHSLVPKAARQLGIDFETLVWRVLEQTL

Sequences:

>Translated_318_residues
MSALVGNARTSDPAAFGRVAVLLGGTSSEREVSLNSGGNVLDALRARGVDAQPVDGIPALAKALVAQQFDRVFNVLHGHN
GGGEDGIVQGLMEAFGVPYTGSDVLGSALSMDKIRTKQVWLSLGLSTPRYARLAAGASAEDIHAAARQIGLPIIVKPANE
GSSVGVSRVFDQAQLEEAVTLAARYDGALLMEQLIEGDELTVAVLGDTALPSIRIVPKGQWYDYNAKYIADDTQYLCPGL
EGEAEAQIRQLALDAFRAAGCRGWGRVDVMRDGSSGQLYLLEVNTAPGMTSHSLVPKAARQLGIDFETLVWRVLEQTL
>Mature_317_residues
SALVGNARTSDPAAFGRVAVLLGGTSSEREVSLNSGGNVLDALRARGVDAQPVDGIPALAKALVAQQFDRVFNVLHGHNG
GGEDGIVQGLMEAFGVPYTGSDVLGSALSMDKIRTKQVWLSLGLSTPRYARLAAGASAEDIHAAARQIGLPIIVKPANEG
SSVGVSRVFDQAQLEEAVTLAARYDGALLMEQLIEGDELTVAVLGDTALPSIRIVPKGQWYDYNAKYIADDTQYLCPGLE
GEAEAQIRQLALDAFRAAGCRGWGRVDVMRDGSSGQLYLLEVNTAPGMTSHSLVPKAARQLGIDFETLVWRVLEQTL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=304, Percent_Identity=53.2894736842105, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1786579, Length=351, Percent_Identity=33.6182336182336, Blast_Score=156, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33688; Mature: 33556

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALVGNARTSDPAAFGRVAVLLGGTSSEREVSLNSGGNVLDALRARGVDAQPVDGIPAL
CCCCCCCCCCCCCHHHCEEEEEEECCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCHHHH
AKALVAQQFDRVFNVLHGHNGGGEDGIVQGLMEAFGVPYTGSDVLGSALSMDKIRTKQVW
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LSLGLSTPRYARLAAGASAEDIHAAARQIGLPIIVKPANEGSSVGVSRVFDQAQLEEAVT
HHCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
LAARYDGALLMEQLIEGDELTVAVLGDTALPSIRIVPKGQWYDYNAKYIADDTQYLCPGL
HHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCEECCCCEEEECCCHHHCCCC
EGEAEAQIRQLALDAFRAAGCRGWGRVDVMRDGSSGQLYLLEVNTAPGMTSHSLVPKAAR
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHH
QLGIDFETLVWRVLEQTL
HHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SALVGNARTSDPAAFGRVAVLLGGTSSEREVSLNSGGNVLDALRARGVDAQPVDGIPAL
CCCCCCCCCCCCHHHCEEEEEEECCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCHHHH
AKALVAQQFDRVFNVLHGHNGGGEDGIVQGLMEAFGVPYTGSDVLGSALSMDKIRTKQVW
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LSLGLSTPRYARLAAGASAEDIHAAARQIGLPIIVKPANEGSSVGVSRVFDQAQLEEAVT
HHCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
LAARYDGALLMEQLIEGDELTVAVLGDTALPSIRIVPKGQWYDYNAKYIADDTQYLCPGL
HHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCEECCCCEEEECCCHHHCCCC
EGEAEAQIRQLALDAFRAAGCRGWGRVDVMRDGSSGQLYLLEVNTAPGMTSHSLVPKAAR
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHH
QLGIDFETLVWRVLEQTL
HHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]