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The map label for this gene is ansA [H]

Identifier: 21241022

GI number: 21241022

Start: 295087

End: 296022

Strand: Direct

Name: ansA [H]

Synonym: XAC0248

Alternate gene names: 21241022

Gene position: 295087-296022 (Clockwise)

Preceding gene: 21241021

Following gene: 21241023

Centisome position: 5.7

GC content: 66.99

Gene sequence:

>936_bases
ATGCTGGTCATCCATGGTGGCGCGGGCGTGGAGAAATCCAGCCTTTCTAAGGACGAGCAGGCGCAGGCGCGCGAGGCGAT
GCAACGCGCACTGCGTGCCGGGCACGCGGTGCTGCAACGTGGCGGCAGTGCAGTGGATGCAGTGGCCGCCACCATCACCG
TGCTGGAGGATGCGCCGCAGTTCAACGCTGGCCGCGGTGCGGTGTTTACCCATGACGGCAGGAACGAACTGGACGCGGCC
ATCATGGATGGCGCCAGCGGCAAGGCCGGTGCGATTACCGGCGTGCACACGGTCAAGAACCCGATCCTGCTGGCACGCAG
CGTGATGGACCGCTCCAAGCACGTGATGCTGGTCGGCGACGGCGCTGAAGCGTTCGCGCGCGAGCAGGGCATCACCCTGG
TAGACCCGGCCTATTTCCGCACCGACAAGCGCTGGCAGCAACTGCAGAAAGCGCTCAAGGCCGAGGCCGGCGACCGCCAG
GCGCAGGCCGAGCTGGATCTGGAGACCGCCAAGCATTTCGGCACCGTCGGCGCGCTGGCGCTGGACCGCGACGGCCATCT
GGCTGCCGGTACCTCCACCGGCGGCATGACCAACAAGCGCTATGGCCGGGTCGGCGATGCACCCATCATCGGGGCCGGCA
CCTATGCCAACAGCCAATGCGCGGTGTCCGGCACCGGCTGGGGCGAGTTCTACATCCGCGCCGTGGCTGCCTACGACATC
TGTGCGCGCATGAAATACGCCGGGCAATCGCTGCAGCAGGCCGCCGAGACCGTGATCGACCAGCAGATCCCGAAGGCCGG
CGGGGACGGCGGCGCCATCGCGCTGGATGCACAGGGCAACCTGGCGTTTCCATTCAATACCGAGGGCATGTACCGCGGCT
GGATCGGCGCCGACGGCACCGCGCATGTGGCGATTTTCAAGGACGAGACGTTGTAA

Upstream 100 bases:

>100_bases
CACTCCATCGAGGAACTTCATGATCGCTAAGTCCGCACGCGCGTTATGCCTGTCGCTCTTTCTGCTGCTCATCGCCGGCC
CGGCGCTTGCAGCCACGCCG

Downstream 100 bases:

>100_bases
CGCCGCCATCCCCAACCGCGGCCTTCCACCCAATCCAACGGCACATGCCAGCGGTCGCGAAACCCGCTAGCGTGCCCGGC
TGCACTACCCACTCAGGAAT

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MLVIHGGAGVEKSSLSKDEQAQAREAMQRALRAGHAVLQRGGSAVDAVAATITVLEDAPQFNAGRGAVFTHDGRNELDAA
IMDGASGKAGAITGVHTVKNPILLARSVMDRSKHVMLVGDGAEAFAREQGITLVDPAYFRTDKRWQQLQKALKAEAGDRQ
AQAELDLETAKHFGTVGALALDRDGHLAAGTSTGGMTNKRYGRVGDAPIIGAGTYANSQCAVSGTGWGEFYIRAVAAYDI
CARMKYAGQSLQQAAETVIDQQIPKAGGDGGAIALDAQGNLAFPFNTEGMYRGWIGADGTAHVAIFKDETL

Sequences:

>Translated_311_residues
MLVIHGGAGVEKSSLSKDEQAQAREAMQRALRAGHAVLQRGGSAVDAVAATITVLEDAPQFNAGRGAVFTHDGRNELDAA
IMDGASGKAGAITGVHTVKNPILLARSVMDRSKHVMLVGDGAEAFAREQGITLVDPAYFRTDKRWQQLQKALKAEAGDRQ
AQAELDLETAKHFGTVGALALDRDGHLAAGTSTGGMTNKRYGRVGDAPIIGAGTYANSQCAVSGTGWGEFYIRAVAAYDI
CARMKYAGQSLQQAAETVIDQQIPKAGGDGGAIALDAQGNLAFPFNTEGMYRGWIGADGTAHVAIFKDETL
>Mature_311_residues
MLVIHGGAGVEKSSLSKDEQAQAREAMQRALRAGHAVLQRGGSAVDAVAATITVLEDAPQFNAGRGAVFTHDGRNELDAA
IMDGASGKAGAITGVHTVKNPILLARSVMDRSKHVMLVGDGAEAFAREQGITLVDPAYFRTDKRWQQLQKALKAEAGDRQ
AQAELDLETAKHFGTVGALALDRDGHLAAGTSTGGMTNKRYGRVGDAPIIGAGTYANSQCAVSGTGWGEFYIRAVAAYDI
CARMKYAGQSLQQAAETVIDQQIPKAGGDGGAIALDAQGNLAFPFNTEGMYRGWIGADGTAHVAIFKDETL

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=312, Percent_Identity=37.5, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI145275200, Length=312, Percent_Identity=37.5, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI98991769, Length=359, Percent_Identity=29.8050139275766, Blast_Score=117, Evalue=9e-27,
Organism=Escherichia coli, GI1787050, Length=311, Percent_Identity=49.1961414790997, Blast_Score=291, Evalue=3e-80,
Organism=Drosophila melanogaster, GI18921183, Length=319, Percent_Identity=36.0501567398119, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=35.0746268656716, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI221330102, Length=272, Percent_Identity=32.3529411764706, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652135, Length=272, Percent_Identity=32.3529411764706, Blast_Score=101, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 32571; Mature: 32571

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVIHGGAGVEKSSLSKDEQAQAREAMQRALRAGHAVLQRGGSAVDAVAATITVLEDAPQ
CEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
FNAGRGAVFTHDGRNELDAAIMDGASGKAGAITGVHTVKNPILLARSVMDRSKHVMLVGD
CCCCCCCEEECCCCCHHHHHHHCCCCCCCCCEECHHHHCCHHHHHHHHHCCCCEEEEEEC
GAEAFAREQGITLVDPAYFRTDKRWQQLQKALKAEAGDRQAQAELDLETAKHFGTVGALA
CHHHHHHHCCCEEECHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
LDRDGHLAAGTSTGGMTNKRYGRVGDAPIIGAGTYANSQCAVSGTGWGEFYIRAVAAYDI
ECCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHHHHH
CARMKYAGQSLQQAAETVIDQQIPKAGGDGGAIALDAQGNLAFPFNTEGMYRGWIGADGT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCEEEEECCCCC
AHVAIFKDETL
EEEEEEECCCC
>Mature Secondary Structure
MLVIHGGAGVEKSSLSKDEQAQAREAMQRALRAGHAVLQRGGSAVDAVAATITVLEDAPQ
CEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
FNAGRGAVFTHDGRNELDAAIMDGASGKAGAITGVHTVKNPILLARSVMDRSKHVMLVGD
CCCCCCCEEECCCCCHHHHHHHCCCCCCCCCEECHHHHCCHHHHHHHHHCCCCEEEEEEC
GAEAFAREQGITLVDPAYFRTDKRWQQLQKALKAEAGDRQAQAELDLETAKHFGTVGALA
CHHHHHHHCCCEEECHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
LDRDGHLAAGTSTGGMTNKRYGRVGDAPIIGAGTYANSQCAVSGTGWGEFYIRAVAAYDI
ECCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHHHHH
CARMKYAGQSLQQAAETVIDQQIPKAGGDGGAIALDAQGNLAFPFNTEGMYRGWIGADGT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCEEEEECCCCC
AHVAIFKDETL
EEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]