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The map label for this gene is nifH [H]

Identifier: 21226821

GI number: 21226821

Start: 866092

End: 866913

Strand: Direct

Name: nifH [H]

Synonym: MM_0719

Alternate gene names: 21226821

Gene position: 866092-866913 (Clockwise)

Preceding gene: 21226819

Following gene: 21226822

Centisome position: 21.14

GC content: 49.39

Gene sequence:

>822_bases
ATGAGACAAATAGCAATCTACGGAAAGGGCGGTATAGGAAAATCCACTACCACGCAGAATCTTACTGCAGCCCTTGCGAC
TATGGGAAACAAAATTCTCCTTGTGGGATGTGACCCCAAAGCAGACTCTACCAGAATGCTGCTTGGCGGCCTGAACCAGA
AAACCGTGCTTGACACACTCAGAAGTGAAGGAGACGAAGGAGTAAACCTTGACGTCGTTGTACAGCCAGGTTTTGGGAAT
ATTAAATGTGTGGAATCAGGAGGGCCCGAGCCCGGTGTAGGATGTGCAGGCAGGGGCATTATTACTTCCATAGGGCTGCT
CGAAAACCTGGGAGCCTACACTGAAGACCTTGACTATGTATTCTACGACGTGCTTGGTGACGTCGTGTGCGGAGGTTTTG
CCATGCCAATCCGTGAAGGAAAAGCCAAGGAGATCTACATTGTTGCCAGTGGGGAACTGATGGCAATTTACGCAGCAAAC
AACATCTGTAAAGGACTTGCCAAGTTTGCCAAAGGTGGAGCCCGTCTTGGAGGCATCATCTGTAACAGCAGAAACGTAGA
CGGAGAACGTGAACTTCTTGAGGCATTCGCGAAGAAACTGGGAAGCCAGCTAATCCATTTCGTGCCCAGGGACAACATCG
TTCAGAGGGCGGAAATCAACAGAAAAACCGTGATCGACTTTGACCCAGAATCCAATCAGGCAAAAGAATACCTGACTCTC
GCCAGCAACGTCCAGAACAACACCAAACTAGTGGTCCCGACTCCGCTCCCTATGGAAGAGTTAGAAGCCATGATGGTGGA
ATTCGGGATTGTTGAGCTATAA

Upstream 100 bases:

>100_bases
GAAAGGAAGTTTTCATTTTATAGAAAAATTTAAATAGTATGAGATAGTACAACGGTTACCGGCTTCCGGTGAAGAAATAA
AACGAGGAGCAAGGTAAAAC

Downstream 100 bases:

>100_bases
TTGCAGAATGCAGGAATTAAGAGCCTTAACTGCTGAGTTAACACCGAACCAGACGGAGGAGATAAAGAAATGCAAATGAT
ACGTGCGATCATAAGGCCCG

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein 2; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MRQIAIYGKGGIGKSTTTQNLTAALATMGNKILLVGCDPKADSTRMLLGGLNQKTVLDTLRSEGDEGVNLDVVVQPGFGN
IKCVESGGPEPGVGCAGRGIITSIGLLENLGAYTEDLDYVFYDVLGDVVCGGFAMPIREGKAKEIYIVASGELMAIYAAN
NICKGLAKFAKGGARLGGIICNSRNVDGERELLEAFAKKLGSQLIHFVPRDNIVQRAEINRKTVIDFDPESNQAKEYLTL
ASNVQNNTKLVVPTPLPMEELEAMMVEFGIVEL

Sequences:

>Translated_273_residues
MRQIAIYGKGGIGKSTTTQNLTAALATMGNKILLVGCDPKADSTRMLLGGLNQKTVLDTLRSEGDEGVNLDVVVQPGFGN
IKCVESGGPEPGVGCAGRGIITSIGLLENLGAYTEDLDYVFYDVLGDVVCGGFAMPIREGKAKEIYIVASGELMAIYAAN
NICKGLAKFAKGGARLGGIICNSRNVDGERELLEAFAKKLGSQLIHFVPRDNIVQRAEINRKTVIDFDPESNQAKEYLTL
ASNVQNNTKLVVPTPLPMEELEAMMVEFGIVEL
>Mature_273_residues
MRQIAIYGKGGIGKSTTTQNLTAALATMGNKILLVGCDPKADSTRMLLGGLNQKTVLDTLRSEGDEGVNLDVVVQPGFGN
IKCVESGGPEPGVGCAGRGIITSIGLLENLGAYTEDLDYVFYDVLGDVVCGGFAMPIREGKAKEIYIVASGELMAIYAAN
NICKGLAKFAKGGARLGGIICNSRNVDGERELLEAFAKKLGSQLIHFVPRDNIVQRAEINRKTVIDFDPESNQAKEYLTL
ASNVQNNTKLVVPTPLPMEELEAMMVEFGIVEL

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 29158; Mature: 29158

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIAIYGKGGIGKSTTTQNLTAALATMGNKILLVGCDPKADSTRMLLGGLNQKTVLDTL
CCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCCHHHHHHHH
RSEGDEGVNLDVVVQPGFGNIKCVESGGPEPGVGCAGRGIITSIGLLENLGAYTEDLDYV
HCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
FYDVLGDVVCGGFAMPIREGKAKEIYIVASGELMAIYAANNICKGLAKFAKGGARLGGII
HHHHHHHHHHCCEEECCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHCCCCEECEEE
CNSRNVDGERELLEAFAKKLGSQLIHFVPRDNIVQRAEINRKTVIDFDPESNQAKEYLTL
ECCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHH
ASNVQNNTKLVVPTPLPMEELEAMMVEFGIVEL
HHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRQIAIYGKGGIGKSTTTQNLTAALATMGNKILLVGCDPKADSTRMLLGGLNQKTVLDTL
CCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCCHHHHHHHH
RSEGDEGVNLDVVVQPGFGNIKCVESGGPEPGVGCAGRGIITSIGLLENLGAYTEDLDYV
HCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
FYDVLGDVVCGGFAMPIREGKAKEIYIVASGELMAIYAANNICKGLAKFAKGGARLGGII
HHHHHHHHHHCCEEECCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHCCCCEECEEE
CNSRNVDGERELLEAFAKKLGSQLIHFVPRDNIVQRAEINRKTVIDFDPESNQAKEYLTL
ECCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHH
ASNVQNNTKLVVPTPLPMEELEAMMVEFGIVEL
HHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2068380; 7961410 [H]