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The map label for this gene is wcaL [C]

Identifier: 21226751

GI number: 21226751

Start: 779325

End: 780449

Strand: Direct

Name: wcaL [C]

Synonym: MM_0649

Alternate gene names: 21226751

Gene position: 779325-780449 (Clockwise)

Preceding gene: 21226742

Following gene: 21226752

Centisome position: 19.02

GC content: 40.36

Gene sequence:

>1125_bases
TTGAAAATAGCCCAGATCTGTCCTCGTTATTCTCCTGATATTGGAGGAGTGGAAACACATGTCAAAGAAATTAGCGAGAG
GCTGGTCAAGGCAGGGCATGATGTTGAGGTTATAACAACGGACCCGAGTGGAAAACTCGAAAAAAGGGACACGATAAATG
GAGTGAAGGTCATAAGGTTCAGGTCTTTCGCCCCAGGAAATGCATACTACTTTGCCCCTCAGATCTATTTTTATCTTAAA
AAGCACAATTTTGATGTGATTCATGCACACAGCTATCACGCTCTTCCGGCTTTATTTGCAGCTCTTGGAAAACGTGAAAG
AAAGTTAGTTTTTACGCCCCATTATCACCGGAGCGGGCATACTGCATTTAGAAACCTGCTGCATAAACCGTACAGGCTCT
TTGGAAAAGTTATATTTTCCAGGTCAGATTCCGTGATATGTGTCTCAGAGTACGAAAAAAGGCTAGTTGAGGCTGATTTT
AGAGTTGAAGGAAAAACTGTAAAAATTCCAAATGGGATCAATCTCAAAGAGTTTGAGCATCTGAGAAATAGTCAAAAAGG
TAAAGGCGTTGAAAAGAAAAAAGGAAAAGAGAAAACTCTCCTTTATGTTGGTCGTCTGGAAGAGTATAAAGGGGTGCAGT
ATATAATTCAAAGCCTGCCTGAACTGAAAGATTTCAGGTTACAAGTCGTTGGAAATGGGCCCTACGAAGAAGAGCTCCGC
AATATGGCAAAAAACCTCGGAGTAGATGAGAGGGTTGAATGGCTGAAAAATCTCTCAAGAGAAGAACTGCTTGAATGCTA
TGCAGATGCCGATATATTTTTGATGCTCTCTTCCCATGAAGCATATGGAATAACGGTTGCTGAGGCACTGGCTGCAGGAA
TACCATGTGTGGTTGCAAAGGGGAGTGCACTTGAAGAGTTTGTTGACGGGGAAAATTGCGCAGGGATTGAGAATCCTGTA
ACAGTAGAAAAAGTGATTGATTCGCTTCAAAAAATTGAGATATTTAAAAGAGAACCGGTTTCTGGGATATTAAAGGGAAT
AATGGACTGGAATGAAGTATCAGCTAGAATAGAAAAAGAGTACATAAGGCAAAGTAATAAGACAAATCATAAATTGTTTA
ATTAA

Upstream 100 bases:

>100_bases
AATGTTTCTGCTGGATGCCATCTATCTTAACTATCACATTATCTTTATATTAAGCTAATGCATTACTCCTTATAATTATT
CGTGTAAAAGAGGTTTATTC

Downstream 100 bases:

>100_bases
AAGTAACAATTTACAAAGTCATTCCTGAATTTTTGGGGAACTTAGAGATGGAAATTGATTATATAAACGGCCTGAAAACG
GACGAAATATTCGGGATGTC

Product: glucosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKIAQICPRYSPDIGGVETHVKEISERLVKAGHDVEVITTDPSGKLEKRDTINGVKVIRFRSFAPGNAYYFAPQIYFYLK
KHNFDVIHAHSYHALPALFAALGKRERKLVFTPHYHRSGHTAFRNLLHKPYRLFGKVIFSRSDSVICVSEYEKRLVEADF
RVEGKTVKIPNGINLKEFEHLRNSQKGKGVEKKKGKEKTLLYVGRLEEYKGVQYIIQSLPELKDFRLQVVGNGPYEEELR
NMAKNLGVDERVEWLKNLSREELLECYADADIFLMLSSHEAYGITVAEALAAGIPCVVAKGSALEEFVDGENCAGIENPV
TVEKVIDSLQKIEIFKREPVSGILKGIMDWNEVSARIEKEYIRQSNKTNHKLFN

Sequences:

>Translated_374_residues
MKIAQICPRYSPDIGGVETHVKEISERLVKAGHDVEVITTDPSGKLEKRDTINGVKVIRFRSFAPGNAYYFAPQIYFYLK
KHNFDVIHAHSYHALPALFAALGKRERKLVFTPHYHRSGHTAFRNLLHKPYRLFGKVIFSRSDSVICVSEYEKRLVEADF
RVEGKTVKIPNGINLKEFEHLRNSQKGKGVEKKKGKEKTLLYVGRLEEYKGVQYIIQSLPELKDFRLQVVGNGPYEEELR
NMAKNLGVDERVEWLKNLSREELLECYADADIFLMLSSHEAYGITVAEALAAGIPCVVAKGSALEEFVDGENCAGIENPV
TVEKVIDSLQKIEIFKREPVSGILKGIMDWNEVSARIEKEYIRQSNKTNHKLFN
>Mature_374_residues
MKIAQICPRYSPDIGGVETHVKEISERLVKAGHDVEVITTDPSGKLEKRDTINGVKVIRFRSFAPGNAYYFAPQIYFYLK
KHNFDVIHAHSYHALPALFAALGKRERKLVFTPHYHRSGHTAFRNLLHKPYRLFGKVIFSRSDSVICVSEYEKRLVEADF
RVEGKTVKIPNGINLKEFEHLRNSQKGKGVEKKKGKEKTLLYVGRLEEYKGVQYIIQSLPELKDFRLQVVGNGPYEEELR
NMAKNLGVDERVEWLKNLSREELLECYADADIFLMLSSHEAYGITVAEALAAGIPCVVAKGSALEEFVDGENCAGIENPV
TVEKVIDSLQKIEIFKREPVSGILKGIMDWNEVSARIEKEYIRQSNKTNHKLFN

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Homo sapiens, GI11863130, Length=384, Percent_Identity=23.4375, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17532709, Length=386, Percent_Identity=25.6476683937824, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI9755344, Length=387, Percent_Identity=24.031007751938, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24654571, Length=322, Percent_Identity=23.9130434782609, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 42642; Mature: 42642

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAQICPRYSPDIGGVETHVKEISERLVKAGHDVEVITTDPSGKLEKRDTINGVKVIRF
CCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEE
RSFAPGNAYYFAPQIYFYLKKHNFDVIHAHSYHALPALFAALGKRERKLVFTPHYHRSGH
ECCCCCCEEEECCEEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCH
TAFRNLLHKPYRLFGKVIFSRSDSVICVSEYEKRLVEADFRVEGKTVKIPNGINLKEFEH
HHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCEECCCEEECCCCCCHHHHHH
LRNSQKGKGVEKKKGKEKTLLYVGRLEEYKGVQYIIQSLPELKDFRLQVVGNGPYEEELR
HHCCCCCCCCHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCHHCCEEEEECCCCHHHHHH
NMAKNLGVDERVEWLKNLSREELLECYADADIFLMLSSHEAYGITVAEALAAGIPCVVAK
HHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEC
GSALEEFVDGENCAGIENPVTVEKVIDSLQKIEIFKREPVSGILKGIMDWNEVSARIEKE
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHH
YIRQSNKTNHKLFN
HHHCCCCCCCCCCC
>Mature Secondary Structure
MKIAQICPRYSPDIGGVETHVKEISERLVKAGHDVEVITTDPSGKLEKRDTINGVKVIRF
CCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEE
RSFAPGNAYYFAPQIYFYLKKHNFDVIHAHSYHALPALFAALGKRERKLVFTPHYHRSGH
ECCCCCCEEEECCEEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCH
TAFRNLLHKPYRLFGKVIFSRSDSVICVSEYEKRLVEADFRVEGKTVKIPNGINLKEFEH
HHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCEECCCEEECCCCCCHHHHHH
LRNSQKGKGVEKKKGKEKTLLYVGRLEEYKGVQYIIQSLPELKDFRLQVVGNGPYEEELR
HHCCCCCCCCHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCHHCCEEEEECCCCHHHHHH
NMAKNLGVDERVEWLKNLSREELLECYADADIFLMLSSHEAYGITVAEALAAGIPCVVAK
HHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCEEEEC
GSALEEFVDGENCAGIENPVTVEKVIDSLQKIEIFKREPVSGILKGIMDWNEVSARIEKE
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHH
YIRQSNKTNHKLFN
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]