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The map label for this gene is frhB [H]

Identifier: 21226729

GI number: 21226729

Start: 757673

End: 758713

Strand: Reverse

Name: frhB [H]

Synonym: MM_0627

Alternate gene names: 21226729

Gene position: 758713-757673 (Counterclockwise)

Preceding gene: 21226730

Following gene: 21226728

Centisome position: 18.52

GC content: 48.41

Gene sequence:

>1041_bases
TTGCCACCAAAGATTGCAGAAGTCATTCAACATGACGTATGCGCAGCCTGTGGGGCATGCGAGGCTGTATGTCCCATTGG
AGCTGTTACAGTAAAGAAGGCGGCAGAAATTCGAGACCCGAACGATCTGAGCCTGTATGAAAAAGGGGCTGCGTTCCAGG
TCTGCGAAGGCTGCCTGACCTGCAGCAGGATCTGTCCCGTAGTGGACGGTTTCATTGAAAACGAGCTTTTAAATGTCCGC
AAATTCTTTGGCGCAAAGTCTAAAGATAATGCCGGCAGCCAGGACGGAGGCGTAACCAGCGGAATTCTCAAAGCCCTCTT
CAATAAAGGTGAAATTGACTGTGCTGTGGGAATCACCCGGAACGAAAACTGGGAACCCGAAGTTGTTCTGCTCACAAGTG
CGGAAGACGTTGAGAGGACAAGAGGAACAAAATATACTTCTGATCCCGTAGTAGCAGCTCTCAGGGAAGCTTTTGAGAAG
TACGACAGGATTGCAGTTGTAGGGGTGCCCTGCCAGGCTCATGCTGCACGTTTGATCCGGGAAAACGTAAACGAGAAGAT
TGTGCTCATTATTGGCCTGCTCTGCATGGAAAGTTTCCATCATGATGTCATGCTCGACAAAATTATCCCTGAGATCATGA
AAGTAAACGTCAGGGATATCGTAAAGATGGAATTCACAAAAGGCAAGTTCTGGGTCTACACAAAGGATGGGGAAGTCCAT
TCCGTGCCTATCAAGGATATTGCAAAGTATGCAAGGAATCCCTGCCATCACTGCTGCGACTATACCTCTGTCTTTGCCGA
TATCTCTGTGGGTTCTGTTGGAGCACCTGACGGGTGGAACTCTGTTTTCATAAGGACTGAAATCGGGGAAAAATACTTCG
ATATGGTCCGGGATGAGATGGAGATAATGGAAGACCCCAAGCCAGGCCTCGAACTTGTTGGAAAGCTTATCGAAATGAAG
CGCAAGGGTAATGCGGAACATTTCCAGGAAGTCTGCAAGGAATTCAGCTTTGAGACCGGAATCCGCAGTGAAACAGTCTG
A

Upstream 100 bases:

>100_bases
TTTATTACATCCTGCAATAATTTTTTTTAATTTATCTCATTATACTTTTAAAAACATCTTGCCAGTAAGGCAAGCTTATC
AAGGCAGTGGAGGTAACTGA

Downstream 100 bases:

>100_bases
AGTCCGAAACTTAATTTAAATTCAAAACTTAATTTAAATCCGAAACTTAATTCAAAAAAGAAATATGGTACATTCGAGGT
TGGATACGATGACGCGAGCT

Product: F420H2 dehydrogenase subunit F

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta; FRH [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MPPKIAEVIQHDVCAACGACEAVCPIGAVTVKKAAEIRDPNDLSLYEKGAAFQVCEGCLTCSRICPVVDGFIENELLNVR
KFFGAKSKDNAGSQDGGVTSGILKALFNKGEIDCAVGITRNENWEPEVVLLTSAEDVERTRGTKYTSDPVVAALREAFEK
YDRIAVVGVPCQAHAARLIRENVNEKIVLIIGLLCMESFHHDVMLDKIIPEIMKVNVRDIVKMEFTKGKFWVYTKDGEVH
SVPIKDIAKYARNPCHHCCDYTSVFADISVGSVGAPDGWNSVFIRTEIGEKYFDMVRDEMEIMEDPKPGLELVGKLIEMK
RKGNAEHFQEVCKEFSFETGIRSETV

Sequences:

>Translated_346_residues
MPPKIAEVIQHDVCAACGACEAVCPIGAVTVKKAAEIRDPNDLSLYEKGAAFQVCEGCLTCSRICPVVDGFIENELLNVR
KFFGAKSKDNAGSQDGGVTSGILKALFNKGEIDCAVGITRNENWEPEVVLLTSAEDVERTRGTKYTSDPVVAALREAFEK
YDRIAVVGVPCQAHAARLIRENVNEKIVLIIGLLCMESFHHDVMLDKIIPEIMKVNVRDIVKMEFTKGKFWVYTKDGEVH
SVPIKDIAKYARNPCHHCCDYTSVFADISVGSVGAPDGWNSVFIRTEIGEKYFDMVRDEMEIMEDPKPGLELVGKLIEMK
RKGNAEHFQEVCKEFSFETGIRSETV
>Mature_345_residues
PPKIAEVIQHDVCAACGACEAVCPIGAVTVKKAAEIRDPNDLSLYEKGAAFQVCEGCLTCSRICPVVDGFIENELLNVRK
FFGAKSKDNAGSQDGGVTSGILKALFNKGEIDCAVGITRNENWEPEVVLLTSAEDVERTRGTKYTSDPVVAALREAFEKY
DRIAVVGVPCQAHAARLIRENVNEKIVLIIGLLCMESFHHDVMLDKIIPEIMKVNVRDIVKMEFTKGKFWVYTKDGEVHS
VPIKDIAKYARNPCHHCCDYTSVFADISVGSVGAPDGWNSVFIRTEIGEKYFDMVRDEMEIMEDPKPGLELVGKLIEMKR
KGNAEHFQEVCKEFSFETGIRSETV

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG1035

COG function: function code C; Coenzyme F420-reducing hydrogenase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the frhB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007516
- InterPro:   IPR017679
- InterPro:   IPR007525 [H]

Pfam domain/function: PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 38360; Mature: 38228

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPKIAEVIQHDVCAACGACEAVCPIGAVTVKKAAEIRDPNDLSLYEKGAAFQVCEGCLT
CCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHH
CSRICPVVDGFIENELLNVRKFFGAKSKDNAGSQDGGVTSGILKALFNKGEIDCAVGITR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECC
NENWEPEVVLLTSAEDVERTRGTKYTSDPVVAALREAFEKYDRIAVVGVPCQAHAARLIR
CCCCCCCEEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHH
ENVNEKIVLIIGLLCMESFHHDVMLDKIIPEIMKVNVRDIVKMEFTKGKFWVYTKDGEVH
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEECCEEEEEECCCCEE
SVPIKDIAKYARNPCHHCCDYTSVFADISVGSVGAPDGWNSVFIRTEIGEKYFDMVRDEM
ECCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
EIMEDPKPGLELVGKLIEMKRKGNAEHFQEVCKEFSFETGIRSETV
HHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PPKIAEVIQHDVCAACGACEAVCPIGAVTVKKAAEIRDPNDLSLYEKGAAFQVCEGCLT
CCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHH
CSRICPVVDGFIENELLNVRKFFGAKSKDNAGSQDGGVTSGILKALFNKGEIDCAVGITR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECC
NENWEPEVVLLTSAEDVERTRGTKYTSDPVVAALREAFEKYDRIAVVGVPCQAHAARLIR
CCCCCCCEEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHH
ENVNEKIVLIIGLLCMESFHHDVMLDKIIPEIMKVNVRDIVKMEFTKGKFWVYTKDGEVH
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEECCEEEEEECCCCEE
SVPIKDIAKYARNPCHHCCDYTSVFADISVGSVGAPDGWNSVFIRTEIGEKYFDMVRDEM
ECCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
EIMEDPKPGLELVGKLIEMKRKGNAEHFQEVCKEFSFETGIRSETV
HHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8521835 [H]