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The map label for this gene is phoB [H]

Identifier: 21226674

GI number: 21226674

Start: 697918

End: 699669

Strand: Reverse

Name: phoB [H]

Synonym: MM_0572

Alternate gene names: 21226674

Gene position: 699669-697918 (Counterclockwise)

Preceding gene: 21226677

Following gene: 21226673

Centisome position: 17.08

GC content: 44.18

Gene sequence:

>1752_bases
ATGGATACTAAAGAAACAAATGTTCTACTAACCCTCCTCGTAGGGCTTCTGCTACTGGGAAATGCTGGTTCTGCAGCAGC
TGTGGAAGATGATTCAAATAGCAAAGATTTTTCAGGTAATTATACCTGTCTCACTGATTCCTTAAAATGTAAATCTTGTC
CAGAATCGGAAGCTAAAATTAAAAACGTGATAATCTTGGTTCCAGATGGGTGTTTTCAGAGTGTCCAGACTCTTGCCAGA
TGGTACAGTGGAAGTCCTCTAATGCTTGATGAAATGGTCGCAGGAACGGTCTCTACTTACAGTACAGACTCAGTTATTAC
TGATTCTTCATCAGCTGCAAGTGCTTTTGCTACCGGATACAAGACAACCAACGGCTTTGTAAGTGTCGGACCGAGCAATG
CTTCAGTTTTAAGTATACTGGAAAACCCTTCTGAAGATATGCAATACAGACCGCTTGCAACAGTGCTTGAAGGGTCAAAG
CTTGAAGGCAAGGCTACAGGGCTTGTAGCAACTTCCCGGGTCACCCATGCTACACCTGCAGCTTTTGCTTCTCACGTGGA
TAACAGGGATAACGAAAACGAGATCATGGAACAGATGGTCTATGAAAATGTTGATGTGGTTTTCGGAGGAGGTTCCCGCC
ATCTAGTGCCTGAAGAAGAAGGTGGAAAAAGGACAGATAGAGAAAACCTGACAGAAGTGCTTCTTGATAGGGGTTACCAG
TATGTTGACAGCAGAGATGAAATGTTGAATCTGTCATCCGGAAAAGCATGGGGCCTTTTTGCCAGCAGTCACATGGCACC
GGATATTGATCGTACCGAACTTGTACCTGAAGAACCCTCTCTTTCAGAAATGACCGGAAAAGCCCTAGAACTGCTTTCAC
AGGACAAAGATGGCTTTTTCCTTCTGGTGGAAGGTAGCCAGGTAGACTGGGCAAATCACGCTAATGACCCTGTTTATGCT
GTTACGGACTTCCTGGCTTTTGATGAAGCTGTTAAGGTTGCAGTTGAATTTGCAGAACAAGATGGACATACCCTTGTCCT
CGCTTTCCCTGACCACAATACAGGCGGGATGACCATTGGTAGTGATTCTGATTCTAATTATACTTCTACAACCATAGAAG
ATGTCATTGATCCTCTCAAAAGCATGAAGCTCAGCTCTGCAGGTATTGCGGAAAAAATAGGAACAGATGTTTCCTCCGAA
AATATTAAGACTCAGCTAAAAACCTGGTGGGGAATTGATGCTACTGATGATGAAGTAACTGAAATTCTTGAAATCTATAA
CAATGGAGAGGGCCTTTCCCTCGATTATGCAATCAGCGAAGTAATCGATCAAAATCATACTGTTATCGGGTGGACTACTC
ATGGACATTGTGGTGAAGATGTCCCTCTCTGGGCTTATGGTCCTGACCACCCCGCAGGTCACCTGGATAACACAGAAATT
GCAGGATATATCGCTGAAAAAGCCGGTTTCAACCTGGAGGAAACAAATAGGTACCTGTTTGTTGAAGTGAGTGAGGTATT
CTCCAGAGACAACGGAGACGGGAAACTGGATGAAAATGAATATCTGCTGGACCTGACAAGCTCCTTAAACCCTGTGCTGA
GGATAGGAGATGCCGAACTACCGGTAAGCACAAATATTCTCATCAAACAAGGCGTAACTTATGAGCTCGAAGGAATAGTC
GTTTATGCTCCGGCGACAGGTAAAGTTTACATCCCTTCCGAAGTGCTTTCACTGGTCAATGGGAAAAAGTAA

Upstream 100 bases:

>100_bases
ATACAGCTGGCTACCTCTTCAAATTTGAGATAAAAAATTTGAGTATAGTCCGAAATAGAGCCTGCTCTTAAAAGCATGTT
CTCAATTTGAGGTAGTTTTA

Downstream 100 bases:

>100_bases
ATAGAGCCGAAGTGAGAAGAGCCTGCCTGAAATTTGAATTAATTAAAGGGGATTATTTCCCTTTTCCTGTTTTTTGGTCG
TGACTTATCAGGAGGCTTGT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MDTKETNVLLTLLVGLLLLGNAGSAAAVEDDSNSKDFSGNYTCLTDSLKCKSCPESEAKIKNVIILVPDGCFQSVQTLAR
WYSGSPLMLDEMVAGTVSTYSTDSVITDSSSAASAFATGYKTTNGFVSVGPSNASVLSILENPSEDMQYRPLATVLEGSK
LEGKATGLVATSRVTHATPAAFASHVDNRDNENEIMEQMVYENVDVVFGGGSRHLVPEEEGGKRTDRENLTEVLLDRGYQ
YVDSRDEMLNLSSGKAWGLFASSHMAPDIDRTELVPEEPSLSEMTGKALELLSQDKDGFFLLVEGSQVDWANHANDPVYA
VTDFLAFDEAVKVAVEFAEQDGHTLVLAFPDHNTGGMTIGSDSDSNYTSTTIEDVIDPLKSMKLSSAGIAEKIGTDVSSE
NIKTQLKTWWGIDATDDEVTEILEIYNNGEGLSLDYAISEVIDQNHTVIGWTTHGHCGEDVPLWAYGPDHPAGHLDNTEI
AGYIAEKAGFNLEETNRYLFVEVSEVFSRDNGDGKLDENEYLLDLTSSLNPVLRIGDAELPVSTNILIKQGVTYELEGIV
VYAPATGKVYIPSEVLSLVNGKK

Sequences:

>Translated_583_residues
MDTKETNVLLTLLVGLLLLGNAGSAAAVEDDSNSKDFSGNYTCLTDSLKCKSCPESEAKIKNVIILVPDGCFQSVQTLAR
WYSGSPLMLDEMVAGTVSTYSTDSVITDSSSAASAFATGYKTTNGFVSVGPSNASVLSILENPSEDMQYRPLATVLEGSK
LEGKATGLVATSRVTHATPAAFASHVDNRDNENEIMEQMVYENVDVVFGGGSRHLVPEEEGGKRTDRENLTEVLLDRGYQ
YVDSRDEMLNLSSGKAWGLFASSHMAPDIDRTELVPEEPSLSEMTGKALELLSQDKDGFFLLVEGSQVDWANHANDPVYA
VTDFLAFDEAVKVAVEFAEQDGHTLVLAFPDHNTGGMTIGSDSDSNYTSTTIEDVIDPLKSMKLSSAGIAEKIGTDVSSE
NIKTQLKTWWGIDATDDEVTEILEIYNNGEGLSLDYAISEVIDQNHTVIGWTTHGHCGEDVPLWAYGPDHPAGHLDNTEI
AGYIAEKAGFNLEETNRYLFVEVSEVFSRDNGDGKLDENEYLLDLTSSLNPVLRIGDAELPVSTNILIKQGVTYELEGIV
VYAPATGKVYIPSEVLSLVNGKK
>Mature_583_residues
MDTKETNVLLTLLVGLLLLGNAGSAAAVEDDSNSKDFSGNYTCLTDSLKCKSCPESEAKIKNVIILVPDGCFQSVQTLAR
WYSGSPLMLDEMVAGTVSTYSTDSVITDSSSAASAFATGYKTTNGFVSVGPSNASVLSILENPSEDMQYRPLATVLEGSK
LEGKATGLVATSRVTHATPAAFASHVDNRDNENEIMEQMVYENVDVVFGGGSRHLVPEEEGGKRTDRENLTEVLLDRGYQ
YVDSRDEMLNLSSGKAWGLFASSHMAPDIDRTELVPEEPSLSEMTGKALELLSQDKDGFFLLVEGSQVDWANHANDPVYA
VTDFLAFDEAVKVAVEFAEQDGHTLVLAFPDHNTGGMTIGSDSDSNYTSTTIEDVIDPLKSMKLSSAGIAEKIGTDVSSE
NIKTQLKTWWGIDATDDEVTEILEIYNNGEGLSLDYAISEVIDQNHTVIGWTTHGHCGEDVPLWAYGPDHPAGHLDNTEI
AGYIAEKAGFNLEETNRYLFVEVSEVFSRDNGDGKLDENEYLLDLTSSLNPVLRIGDAELPVSTNILIKQGVTYELEGIV
VYAPATGKVYIPSEVLSLVNGKK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI94721246, Length=347, Percent_Identity=30.835734870317, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI157266296, Length=347, Percent_Identity=30.835734870317, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI157266292, Length=348, Percent_Identity=29.5977011494253, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI116734717, Length=338, Percent_Identity=29.2899408284024, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI294660772, Length=290, Percent_Identity=29.3103448275862, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI294660770, Length=290, Percent_Identity=29.3103448275862, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI48994877, Length=404, Percent_Identity=28.7128712871287, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320689, Length=493, Percent_Identity=33.2657200811359, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI21355151, Length=331, Percent_Identity=30.8157099697885, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI19921912, Length=340, Percent_Identity=31.1764705882353, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI85725200, Length=342, Percent_Identity=27.7777777777778, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI85815833, Length=342, Percent_Identity=27.7777777777778, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21355981, Length=331, Percent_Identity=29.0030211480363, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21358067, Length=458, Percent_Identity=26.8558951965066, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24651554, Length=350, Percent_Identity=28.8571428571429, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI18859923, Length=318, Percent_Identity=29.874213836478, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI21355149, Length=342, Percent_Identity=29.8245614035088, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24585213, Length=355, Percent_Identity=29.5774647887324, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24657835, Length=340, Percent_Identity=29.1176470588235, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24651556, Length=301, Percent_Identity=29.5681063122924, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24657827, Length=342, Percent_Identity=28.0701754385965, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24657842, Length=340, Percent_Identity=28.5294117647059, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 63057; Mature: 63057

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTKETNVLLTLLVGLLLLGNAGSAAAVEDDSNSKDFSGNYTCLTDSLKCKSCPESEAKI
CCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
KNVIILVPDGCFQSVQTLARWYSGSPLMLDEMVAGTVSTYSTDSVITDSSSAASAFATGY
CEEEEEECCHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCCCCCEEECCCCHHHHHHCCC
KTTNGFVSVGPSNASVLSILENPSEDMQYRPLATVLEGSKLEGKATGLVATSRVTHATPA
CCCCCEEEECCCCCEEHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCCHH
AFASHVDNRDNENEIMEQMVYENVDVVFGGGSRHLVPEEEGGKRTDRENLTEVLLDRGYQ
HHHHHCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCH
YVDSRDEMLNLSSGKAWGLFASSHMAPDIDRTELVPEEPSLSEMTGKALELLSQDKDGFF
HHCCHHHEEECCCCCEEEEEECCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LLVEGSQVDWANHANDPVYAVTDFLAFDEAVKVAVEFAEQDGHTLVLAFPDHNTGGMTIG
EEEECCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEC
SDSDSNYTSTTIEDVIDPLKSMKLSSAGIAEKIGTDVSSENIKTQLKTWWGIDATDDEVT
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHEECCCCCHHHHH
EILEIYNNGEGLSLDYAISEVIDQNHTVIGWTTHGHCGEDVPLWAYGPDHPAGHLDNTEI
HHHHHHCCCCCCEEHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHH
AGYIAEKAGFNLEETNRYLFVEVSEVFSRDNGDGKLDENEYLLDLTSSLNPVLRIGDAEL
HHEEHHHCCCCHHHCCCEEEEEEHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCC
PVSTNILIKQGVTYELEGIVVYAPATGKVYIPSEVLSLVNGKK
CCCCCEEEECCCEEEECEEEEEECCCCCEEECHHHHHHHCCCC
>Mature Secondary Structure
MDTKETNVLLTLLVGLLLLGNAGSAAAVEDDSNSKDFSGNYTCLTDSLKCKSCPESEAKI
CCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
KNVIILVPDGCFQSVQTLARWYSGSPLMLDEMVAGTVSTYSTDSVITDSSSAASAFATGY
CEEEEEECCHHHHHHHHHHHHHCCCCEEHHHHHHCCCCCCCCCCEEECCCCHHHHHHCCC
KTTNGFVSVGPSNASVLSILENPSEDMQYRPLATVLEGSKLEGKATGLVATSRVTHATPA
CCCCCEEEECCCCCEEHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCCHH
AFASHVDNRDNENEIMEQMVYENVDVVFGGGSRHLVPEEEGGKRTDRENLTEVLLDRGYQ
HHHHHCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCH
YVDSRDEMLNLSSGKAWGLFASSHMAPDIDRTELVPEEPSLSEMTGKALELLSQDKDGFF
HHCCHHHEEECCCCCEEEEEECCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LLVEGSQVDWANHANDPVYAVTDFLAFDEAVKVAVEFAEQDGHTLVLAFPDHNTGGMTIG
EEEECCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEC
SDSDSNYTSTTIEDVIDPLKSMKLSSAGIAEKIGTDVSSENIKTQLKTWWGIDATDDEVT
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHEECCCCCHHHHH
EILEIYNNGEGLSLDYAISEVIDQNHTVIGWTTHGHCGEDVPLWAYGPDHPAGHLDNTEI
HHHHHHCCCCCCEEHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCHH
AGYIAEKAGFNLEETNRYLFVEVSEVFSRDNGDGKLDENEYLLDLTSSLNPVLRIGDAEL
HHEEHHHCCCCHHHCCCEEEEEEHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCC
PVSTNILIKQGVTYELEGIVVYAPATGKVYIPSEVLSLVNGKK
CCCCCEEEECCCEEEECEEEEEECCCCCEEECHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]