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The map label for this gene is ackA [H]

Identifier: 21226597

GI number: 21226597

Start: 616158

End: 617402

Strand: Reverse

Name: ackA [H]

Synonym: MM_0495

Alternate gene names: 21226597

Gene position: 617402-616158 (Counterclockwise)

Preceding gene: 21226598

Following gene: 21226596

Centisome position: 15.07

GC content: 46.35

Gene sequence:

>1245_bases
TTGAACTGGGGTTCACAAATGAAAGTACTGGTTATAAACGCAGGGAGTTCTTCTCTCAAATATCAATTAATTGATATGAC
AAATGAGTCCGCTCTTGCAATAGGGCTCTGTGAGAGAATAGGTATCGATAACTCTATTATCACCCAGAAGAGGTTTGACG
GCAAAAAGCTGGAAAAACAGACCGATCTTCCCAACCACAAGATCGCACTCGAAGAAGTTGTCAAGGCTCTCACAGATTCT
GAATTCGGTGTTATAAAGAGCATGGACGAGATCAATGCTGTCGGGCACAGAGTTGTGCACGGTGGGGAAAAATTCAATTC
CTCGGCTTTGATCAATGAAGGCGTAGAACAAGCAATCAAGGACTGCTTCGAACTGGCACCTCTTCATAACCCTCCAAACA
TGATGGGAATTTCTTCCTGTCAGGAAATTATGCCTGGGGTCCCGATGGTTGCTGTCTTTGATACGGCATTCCACCATACA
ATCCCGCCATATGCTTACATGTATGCTCTGCCGTATGAACTGTACGAAAAGTACGGGATCAGAAAATATGGTTTCCACGG
CACCTCTCACTTCTATGTTGCCAAGAGGGCTGCCGCTATGCTCGGAAAACCCGAACAGGATGTCAAGGTAATCACCTGCC
ACCTTGGTAACGGCTCAAGCATTACAGCTGTTAAAGGCGGAAAATCCATTGAAACTACTATGGGCTTTACCCCTCTTGAG
GGAGTTGCAATGGGAACCAGGTGCGGTTCTATTGACCCCGCAGTTGTCCCCTTCATCATGGAAAAGGAAGGACTGTCAAC
CAGAGAGATTGATACCCTTATGAACAAGAAGTCGGGTGTGCTTGGGGTTTCCAGTCTGAGCAACGACTTCAGAGATCTTG
ACGAAGCAGCTTCCAAAGGCAACCAGAAAGCCGAACTTGCCCTTGAGATCTTCGCATATAAGATCAAGAAAGTCATAGGT
GAGTATATAGCCGTTCTCAATGGTGTAGACGCAATAGTCTTTACCGCAGGTATCGGAGAAAACAGCGCAAGCATCAGAAA
GAGAATCCTTGCTGACCTTGACGGAATTGGCATCAAGATCGACGAAGAGAAGAACAAGATCAGAGGCCAGGAAATCGACA
TCAGCACTCCGGATGCAACAGTCAGGGTCCTTGTTATCCCAACCAATGAAGAACTGACCATTGCAAGGGACACAAAGGAA
ATCTGTGAAACCGAAGTAAAGCTGAGAAGCTCAGTGCCTATCTGA

Upstream 100 bases:

>100_bases
GCGCAGGATAAATAATATTAGATGTTAAAACAGCGTTTTATTAACTTTCATATATAGTAACATTTATCCGGAAACTCTAA
ATGTAATTGAAAGTAATGGA

Downstream 100 bases:

>100_bases
TGGTAAAGGAACCTTTCCTTTGCCTTTTATTATCTTTTTTACCCCGAATTGCGGCTCGGGAGTACGCTGTCCTTCCCGAA
TTGCGGCTCGGGAGTACGCT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MNWGSQMKVLVINAGSSSLKYQLIDMTNESALAIGLCERIGIDNSIITQKRFDGKKLEKQTDLPNHKIALEEVVKALTDS
EFGVIKSMDEINAVGHRVVHGGEKFNSSALINEGVEQAIKDCFELAPLHNPPNMMGISSCQEIMPGVPMVAVFDTAFHHT
IPPYAYMYALPYELYEKYGIRKYGFHGTSHFYVAKRAAAMLGKPEQDVKVITCHLGNGSSITAVKGGKSIETTMGFTPLE
GVAMGTRCGSIDPAVVPFIMEKEGLSTREIDTLMNKKSGVLGVSSLSNDFRDLDEAASKGNQKAELALEIFAYKIKKVIG
EYIAVLNGVDAIVFTAGIGENSASIRKRILADLDGIGIKIDEEKNKIRGQEIDISTPDATVRVLVIPTNEELTIARDTKE
ICETEVKLRSSVPI

Sequences:

>Translated_414_residues
MNWGSQMKVLVINAGSSSLKYQLIDMTNESALAIGLCERIGIDNSIITQKRFDGKKLEKQTDLPNHKIALEEVVKALTDS
EFGVIKSMDEINAVGHRVVHGGEKFNSSALINEGVEQAIKDCFELAPLHNPPNMMGISSCQEIMPGVPMVAVFDTAFHHT
IPPYAYMYALPYELYEKYGIRKYGFHGTSHFYVAKRAAAMLGKPEQDVKVITCHLGNGSSITAVKGGKSIETTMGFTPLE
GVAMGTRCGSIDPAVVPFIMEKEGLSTREIDTLMNKKSGVLGVSSLSNDFRDLDEAASKGNQKAELALEIFAYKIKKVIG
EYIAVLNGVDAIVFTAGIGENSASIRKRILADLDGIGIKIDEEKNKIRGQEIDISTPDATVRVLVIPTNEELTIARDTKE
ICETEVKLRSSVPI
>Mature_414_residues
MNWGSQMKVLVINAGSSSLKYQLIDMTNESALAIGLCERIGIDNSIITQKRFDGKKLEKQTDLPNHKIALEEVVKALTDS
EFGVIKSMDEINAVGHRVVHGGEKFNSSALINEGVEQAIKDCFELAPLHNPPNMMGISSCQEIMPGVPMVAVFDTAFHHT
IPPYAYMYALPYELYEKYGIRKYGFHGTSHFYVAKRAAAMLGKPEQDVKVITCHLGNGSSITAVKGGKSIETTMGFTPLE
GVAMGTRCGSIDPAVVPFIMEKEGLSTREIDTLMNKKSGVLGVSSLSNDFRDLDEAASKGNQKAELALEIFAYKIKKVIG
EYIAVLNGVDAIVFTAGIGENSASIRKRILADLDGIGIKIDEEKNKIRGQEIDISTPDATVRVLVIPTNEELTIARDTKE
ICETEVKLRSSVPI

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=43.609022556391, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI145693184, Length=393, Percent_Identity=40.9669211195929, Blast_Score=312, Evalue=2e-86,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 45159; Mature: 45159

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWGSQMKVLVINAGSSSLKYQLIDMTNESALAIGLCERIGIDNSIITQKRFDGKKLEKQ
CCCCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEHHHHHCCCCCCCCCCCCCCHHHHHH
TDLPNHKIALEEVVKALTDSEFGVIKSMDEINAVGHRVVHGGEKFNSSALINEGVEQAIK
CCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DCFELAPLHNPPNMMGISSCQEIMPGVPMVAVFDTAFHHTIPPYAYMYALPYELYEKYGI
HHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEHHHHHHHCCCCCHHHHHHCCHHHHHHCCC
RKYGFHGTSHFYVAKRAAAMLGKPEQDVKVITCHLGNGSSITAVKGGKSIETTMGFTPLE
EEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCCEEHHCCCCCCC
GVAMGTRCGSIDPAVVPFIMEKEGLSTREIDTLMNKKSGVLGVSSLSNDFRDLDEAASKG
CEEECCCCCCCCHHHHHEEECCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCC
NQKAELALEIFAYKIKKVIGEYIAVLNGVDAIVFTAGIGENSASIRKRILADLDGIGIKI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEE
DEEKNKIRGQEIDISTPDATVRVLVIPTNEELTIARDTKEICETEVKLRSSVPI
ECCHHHCCCCEEECCCCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNWGSQMKVLVINAGSSSLKYQLIDMTNESALAIGLCERIGIDNSIITQKRFDGKKLEKQ
CCCCCCEEEEEEECCCCCEEEEEEECCCCCCCEEEHHHHHCCCCCCCCCCCCCCHHHHHH
TDLPNHKIALEEVVKALTDSEFGVIKSMDEINAVGHRVVHGGEKFNSSALINEGVEQAIK
CCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DCFELAPLHNPPNMMGISSCQEIMPGVPMVAVFDTAFHHTIPPYAYMYALPYELYEKYGI
HHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEHHHHHHHCCCCCHHHHHHCCHHHHHHCCC
RKYGFHGTSHFYVAKRAAAMLGKPEQDVKVITCHLGNGSSITAVKGGKSIETTMGFTPLE
EEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCCEEHHCCCCCCC
GVAMGTRCGSIDPAVVPFIMEKEGLSTREIDTLMNKKSGVLGVSSLSNDFRDLDEAASKG
CEEECCCCCCCCHHHHHEEECCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCC
NQKAELALEIFAYKIKKVIGEYIAVLNGVDAIVFTAGIGENSASIRKRILADLDGIGIKI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEE
DEEKNKIRGQEIDISTPDATVRVLVIPTNEELTIARDTKEICETEVKLRSSVPI
ECCHHHCCCCEEECCCCCCEEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]