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The map label for this gene is nadE

Identifier: 21226548

GI number: 21226548

Start: 567493

End: 568263

Strand: Reverse

Name: nadE

Synonym: MM_0446

Alternate gene names: 21226548

Gene position: 568263-567493 (Counterclockwise)

Preceding gene: 21226554

Following gene: 21226546

Centisome position: 13.87

GC content: 51.36

Gene sequence:

>771_bases
ATGGACCTTGAAAACGCACAGAACAGGATTATTGATTTTATCCGGGACGAGACTGGCAGAGCAGGCGTAAAAGGGGTAGT
CGTTGGCATCAGCGGAGGGATTGATTCCGCCCTTACCGCAACTCTTGCCGTAAAAGCTCTCGGAAAGGACAGGGTTCTTG
GCATCCACATGCCTGAGAGCAGCCTTACCCCTGCCGTTGACAGTGAGGACGCTGAAGCTCTGGCAGACTGGCTCGGGATA
GAGTACAGGACAATTGACATCTCAGGCATTATCTCGGCTTTTATGGCTGCGGTCCCGGAGAGCGAATCTGCGGACAGGCT
CACGAAAGGCAACCTGAAAGCAAGGACCCGGATGTCCCTTCTTTATTTCCATGCAAACCGGCTGAACCGCATGGTTATCG
GGACAGGAAACAAAACCGAGATCCTTCTCGGCTACTACACGAAATACGGGGATGGAGGCGTTGACCTTGAACCTATAGGA
GGGCTTTATAAAACCGGGGTATGGGAGCTTTCCAGCAGGCTGGGAATCCCGGAATCTCTCATCACCAAAAAACCGTCCGC
AGGTCTATGGGCAGGCCAGACAGATGAAGCAGATCTCGGCATCTCATACGTGAAGGTTGATGAAGTTCTGAAAATGATTG
AGGATGGCGTAGAACCGGAAGTAATTCTGGATAAGACAGGGATTTCTGCAGATCAGCTTAACTCTGTCACCAGGCGCATT
GAGAGAAATGAACACAAAAGAAAAGCTCCCCCGGTGCCGGAACTCTATTGA

Upstream 100 bases:

>100_bases
GATGTTATCGTTCAGACTCTCCAGCAAAGCAACCTTAGTTTAAATTTTAGATTAAAATATTTGAATTCACACGATTCACA
AATTTCAGGAATTTAAGATT

Downstream 100 bases:

>100_bases
ATTTACTGGTCAACTCCATTGAATTTGCTGGTTAACTCCATTGAACTGACTTGTTAACTTCATTGGATTCGCCTGTTAAC
TTTATTGAATTCACCGGTTA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MDLENAQNRIIDFIRDETGRAGVKGVVVGISGGIDSALTATLAVKALGKDRVLGIHMPESSLTPAVDSEDAEALADWLGI
EYRTIDISGIISAFMAAVPESESADRLTKGNLKARTRMSLLYFHANRLNRMVIGTGNKTEILLGYYTKYGDGGVDLEPIG
GLYKTGVWELSSRLGIPESLITKKPSAGLWAGQTDEADLGISYVKVDEVLKMIEDGVEPEVILDKTGISADQLNSVTRRI
ERNEHKRKAPPVPELY

Sequences:

>Translated_256_residues
MDLENAQNRIIDFIRDETGRAGVKGVVVGISGGIDSALTATLAVKALGKDRVLGIHMPESSLTPAVDSEDAEALADWLGI
EYRTIDISGIISAFMAAVPESESADRLTKGNLKARTRMSLLYFHANRLNRMVIGTGNKTEILLGYYTKYGDGGVDLEPIG
GLYKTGVWELSSRLGIPESLITKKPSAGLWAGQTDEADLGISYVKVDEVLKMIEDGVEPEVILDKTGISADQLNSVTRRI
ERNEHKRKAPPVPELY
>Mature_256_residues
MDLENAQNRIIDFIRDETGRAGVKGVVVGISGGIDSALTATLAVKALGKDRVLGIHMPESSLTPAVDSEDAEALADWLGI
EYRTIDISGIISAFMAAVPESESADRLTKGNLKARTRMSLLYFHANRLNRMVIGTGNKTEILLGYYTKYGDGGVDLEPIG
GLYKTGVWELSSRLGIPESLITKKPSAGLWAGQTDEADLGISYVKVDEVLKMIEDGVEPEVILDKTGISADQLNSVTRRI
ERNEHKRKAPPVPELY

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=261, Percent_Identity=31.0344827586207, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_METMA (Q8PZP6)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632470.1
- ProteinModelPortal:   Q8PZP6
- SMR:   Q8PZP6
- GeneID:   1478788
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0446
- NMPDR:   fig|192952.1.peg.446
- HOGENOM:   HBG351567
- OMA:   FRYARDW
- ProtClustDB:   PRK13980
- BioCyc:   MMAZ192952:MM0446-MONOMER
- BRENDA:   6.3.1.5
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 27786; Mature: 27786

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: ACT_SITE 31-31

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLENAQNRIIDFIRDETGRAGVKGVVVGISGGIDSALTATLAVKALGKDRVLGIHMPES
CCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCC
SLTPAVDSEDAEALADWLGIEYRTIDISGIISAFMAAVPESESADRLTKGNLKARTRMSL
CCCCCCCCCHHHHHHHHHCCEEEEEEHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHH
LYFHANRLNRMVIGTGNKTEILLGYYTKYGDGGVDLEPIGGLYKTGVWELSSRLGIPESL
EEEECCCCCEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
ITKKPSAGLWAGQTDEADLGISYVKVDEVLKMIEDGVEPEVILDKTGISADQLNSVTRRI
HCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
ERNEHKRKAPPVPELY
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDLENAQNRIIDFIRDETGRAGVKGVVVGISGGIDSALTATLAVKALGKDRVLGIHMPES
CCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCC
SLTPAVDSEDAEALADWLGIEYRTIDISGIISAFMAAVPESESADRLTKGNLKARTRMSL
CCCCCCCCCHHHHHHHHHCCEEEEEEHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHH
LYFHANRLNRMVIGTGNKTEILLGYYTKYGDGGVDLEPIGGLYKTGVWELSSRLGIPESL
EEEECCCCCEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
ITKKPSAGLWAGQTDEADLGISYVKVDEVLKMIEDGVEPEVILDKTGISADQLNSVTRRI
HCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
ERNEHKRKAPPVPELY
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824