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The map label for this gene is leuC1 [H]

Identifier: 21226511

GI number: 21226511

Start: 520855

End: 522030

Strand: Direct

Name: leuC1 [H]

Synonym: MM_0409

Alternate gene names: 21226511

Gene position: 520855-522030 (Clockwise)

Preceding gene: 21226509

Following gene: 21226512

Centisome position: 12.72

GC content: 50.0

Gene sequence:

>1176_bases
GTGCTGGCAGATGTGGACTATGCGATGGCGCATGACGGCACATCAATACTTGCGGTAAATGCTTTTAAGGAAATGGAGAT
GGAAAGGGTCTGGGACCCCTCAAGAATTGTGATTCCTTTTGACCATATTGCCCCTGCAAACACCGAGACATCAGCTACCC
TGCAAAAAGAGATCCGAGAATGGGTAAGGGAACAGAGTATACCCAATTTCTATGAAATAGGAGAAGGGATCTGCCATCAG
GTTCTTCCTGAAAACGGCTTTGCACTGCCTGGAAAACTGCTCGTCGGAGCTGACTCTCATTCCTGTACATACGGGGCTTT
CGGGGCTTTTGCAACTGGAGTCGGGGCAACCGATATGGCTGAAATCTTTGCTACGGGAAAGCTCTGGTTCAAGGTACCGG
AAAGCTTCAGGATGACAGTTGAAGGAAGCCTTGACAAACACGTTTACGCAAAAGACCTTACCCTTTACCTGATAGGAAAA
ACAGGAATTGCAGGTGCAACCTATAAAGCAGTTGAGTTTTACGGGCAGGCAATCAGCGAACTTTCTGTTGCCGGCAGGAT
GACGCTCTGCAACATGGCAATCGAGATGGGAGCAAAGACCGGAATAGTCCCTCCCGACGAAAAGACCTTTGATTTCCTGA
AAAACAGGGCAGTTGCTCCTTATGAACCTGTGTATTCAGACCCTGATGCTTCTTATCTTAAAGAGTTTGTTTATGATGCC
GGAGACATAGAGCCCCAGGTCGCCTGCCCCCACCAGGTGGATAACGTAAAGCCAGTAGGGGAGGTTGAAGGGACTCACGT
GGACCAGGTCTTTATAGGGACGTGCACCAACGGAAGGCTTGAAGACCTCGAAGTAGCAGCATCAGTCCTGAAAGGAAAAA
AGGTTACAGTCAGGACAATCATAATCCCTGCATCCCGCTCAACCCTCCTTGCAGCAATCAAGAATGGGACAATGGAAATC
CTGCTGAAAGCCGGTGTGACCCTTGCAACCCCTGGCTGCGGGCCCTGCCTTGGCGCTCATCAGGGCGTTCTTGGGGAAGG
CGAAGTCTGTGTTTCAACCGCAAACAGGAACTTTAAAGGCAGGATGGGAAAAGACGGCTTTATCTATCTTGCGTCTCCCG
CAACCGCAGCAGCTTCTGCACTTACAGGCGAAATCACTGACCCGAGGAAGATATAA

Upstream 100 bases:

>100_bases
AATTATCATTCAATATTGGAAAGGAGGCTTACTTCTGGGAACAATCAGCGAAAAAATCTTTTCCCGGGCAGCAGGAAAAG
AGGTAAAGGCTAATGATTTT

Downstream 100 bases:

>100_bases
AAGAAGAAAAAATCTAAAAGAAGAAAAAATCTAAGAGAAGAAAAAATCTAAGAGAAGAAAAATCTGAAAGGCAGTCTTCA
CGGCTGCCCTCTCGATTCTT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MLADVDYAMAHDGTSILAVNAFKEMEMERVWDPSRIVIPFDHIAPANTETSATLQKEIREWVREQSIPNFYEIGEGICHQ
VLPENGFALPGKLLVGADSHSCTYGAFGAFATGVGATDMAEIFATGKLWFKVPESFRMTVEGSLDKHVYAKDLTLYLIGK
TGIAGATYKAVEFYGQAISELSVAGRMTLCNMAIEMGAKTGIVPPDEKTFDFLKNRAVAPYEPVYSDPDASYLKEFVYDA
GDIEPQVACPHQVDNVKPVGEVEGTHVDQVFIGTCTNGRLEDLEVAASVLKGKKVTVRTIIIPASRSTLLAAIKNGTMEI
LLKAGVTLATPGCGPCLGAHQGVLGEGEVCVSTANRNFKGRMGKDGFIYLASPATAAASALTGEITDPRKI

Sequences:

>Translated_391_residues
MLADVDYAMAHDGTSILAVNAFKEMEMERVWDPSRIVIPFDHIAPANTETSATLQKEIREWVREQSIPNFYEIGEGICHQ
VLPENGFALPGKLLVGADSHSCTYGAFGAFATGVGATDMAEIFATGKLWFKVPESFRMTVEGSLDKHVYAKDLTLYLIGK
TGIAGATYKAVEFYGQAISELSVAGRMTLCNMAIEMGAKTGIVPPDEKTFDFLKNRAVAPYEPVYSDPDASYLKEFVYDA
GDIEPQVACPHQVDNVKPVGEVEGTHVDQVFIGTCTNGRLEDLEVAASVLKGKKVTVRTIIIPASRSTLLAAIKNGTMEI
LLKAGVTLATPGCGPCLGAHQGVLGEGEVCVSTANRNFKGRMGKDGFIYLASPATAAASALTGEITDPRKI
>Mature_391_residues
MLADVDYAMAHDGTSILAVNAFKEMEMERVWDPSRIVIPFDHIAPANTETSATLQKEIREWVREQSIPNFYEIGEGICHQ
VLPENGFALPGKLLVGADSHSCTYGAFGAFATGVGATDMAEIFATGKLWFKVPESFRMTVEGSLDKHVYAKDLTLYLIGK
TGIAGATYKAVEFYGQAISELSVAGRMTLCNMAIEMGAKTGIVPPDEKTFDFLKNRAVAPYEPVYSDPDASYLKEFVYDA
GDIEPQVACPHQVDNVKPVGEVEGTHVDQVFIGTCTNGRLEDLEVAASVLKGKKVTVRTIIIPASRSTLLAAIKNGTMEI
LLKAGVTLATPGCGPCLGAHQGVLGEGEVCVSTANRNFKGRMGKDGFIYLASPATAAASALTGEITDPRKI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=415, Percent_Identity=28.6746987951807, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI8659555, Length=149, Percent_Identity=28.8590604026846, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1786259, Length=441, Percent_Identity=36.734693877551, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI87081781, Length=353, Percent_Identity=25.2124645892351, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI2367097, Length=359, Percent_Identity=26.4623955431755, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787531, Length=393, Percent_Identity=25.1908396946565, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=344, Percent_Identity=30.5232558139535, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=344, Percent_Identity=30.5232558139535, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=300, Percent_Identity=28, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17568399, Length=402, Percent_Identity=23.8805970149254, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321429, Length=391, Percent_Identity=35.5498721227622, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6320440, Length=416, Percent_Identity=34.375, Blast_Score=212, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6323335, Length=419, Percent_Identity=28.1622911694511, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322261, Length=347, Percent_Identity=28.8184438040346, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281365315, Length=416, Percent_Identity=26.2019230769231, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI17864292, Length=416, Percent_Identity=26.2019230769231, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI161076999, Length=411, Percent_Identity=26.2773722627737, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI28571643, Length=415, Percent_Identity=26.2650602409639, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24645686, Length=401, Percent_Identity=26.6832917705736, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17137564, Length=402, Percent_Identity=25.1243781094527, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 41938; Mature: 41938

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLADVDYAMAHDGTSILAVNAFKEMEMERVWDPSRIVIPFDHIAPANTETSATLQKEIRE
CCCCCCHHHHCCCCEEEEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
WVREQSIPNFYEIGEGICHQVLPENGFALPGKLLVGADSHSCTYGAFGAFATGVGATDMA
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCHHHH
EIFATGKLWFKVPESFRMTVEGSLDKHVYAKDLTLYLIGKTGIAGATYKAVEFYGQAISE
HHHHCCEEEEECCCCEEEEEECCCCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHH
LSVAGRMTLCNMAIEMGAKTGIVPPDEKTFDFLKNRAVAPYEPVYSDPDASYLKEFVYDA
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
GDIEPQVACPHQVDNVKPVGEVEGTHVDQVFIGTCTNGRLEDLEVAASVLKGKKVTVRTI
CCCCCCEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEE
IIPASRSTLLAAIKNGTMEILLKAGVTLATPGCGPCLGAHQGVLGEGEVCVSTANRNFKG
EEECCCCCEEHHHHCCCEEEHEECCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
RMGKDGFIYLASPATAAASALTGEITDPRKI
CCCCCCEEEEECCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLADVDYAMAHDGTSILAVNAFKEMEMERVWDPSRIVIPFDHIAPANTETSATLQKEIRE
CCCCCCHHHHCCCCEEEEEHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHH
WVREQSIPNFYEIGEGICHQVLPENGFALPGKLLVGADSHSCTYGAFGAFATGVGATDMA
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCCHHHH
EIFATGKLWFKVPESFRMTVEGSLDKHVYAKDLTLYLIGKTGIAGATYKAVEFYGQAISE
HHHHCCEEEEECCCCEEEEEECCCCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHH
LSVAGRMTLCNMAIEMGAKTGIVPPDEKTFDFLKNRAVAPYEPVYSDPDASYLKEFVYDA
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
GDIEPQVACPHQVDNVKPVGEVEGTHVDQVFIGTCTNGRLEDLEVAASVLKGKKVTVRTI
CCCCCCEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEE
IIPASRSTLLAAIKNGTMEILLKAGVTLATPGCGPCLGAHQGVLGEGEVCVSTANRNFKG
EEECCCCCEEHHHHCCCEEEHEECCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
RMGKDGFIYLASPATAAASALTGEITDPRKI
CCCCCCEEEEECCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]