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The map label for this gene is gdhA-2 [H]

Identifier: 21226459

GI number: 21226459

Start: 464319

End: 465443

Strand: Direct

Name: gdhA-2 [H]

Synonym: MM_0357

Alternate gene names: 21226459

Gene position: 464319-465443 (Clockwise)

Preceding gene: 21226458

Following gene: 21226462

Centisome position: 11.33

GC content: 47.11

Gene sequence:

>1125_bases
ATGTTCAGATTTGCAGATGAACTGGGGCCATTTAAGATAATTCACATTTATGAACCGTCTATTGACCTGAAAGCAGTGCT
CGTTGTTGATAATATTGCAAGGGGACCAGCCCTTGGAGGGGTAAGAATAGCTCCGGACGTGAGTGCCGAAGAATGCTTCA
GGCTGGCGAGGGCCATGACCCTTAAAAATGCGGCTGCAGACCTTCCTTATGGGGGCGGTAAAATTGTCGTTTACGGGGAC
CCGAAAATGCCTTTCGAAAAGAAAAGCCAGCTACTGAGGGCTCTTGCAGGTGCTCTCCGGTACACAGAGGAATATATCTT
TGCCCCTGACATGGGGACAGATGAAATATGCATGGCCTGCATAAAAGATGAAATCGGAAGGGTTGTGGGTCTTCCCTGTG
AGATGGGAGGCATCCCTCTTGATGAGGTTGGAGCTACAGGCTGGGGGCTTTTTAATGCAACAGAAGTCGCACTTAAGTAT
TGCGATTTTGAGCTGAAAGGAGCCAGGGTAGTTATTCAGGGCTTCGGCGCAGTCGGAAAACACGCTGCCCGCTTCCTTGT
AAGGAAAGGAGCTGTCCTTGTAGGAGTCGCCGATTCCAGAGGTGCAGTCCATAACCCTGAGGGGCTTGATGTTGATTCTC
TGATCGCACTTAAAAATGAAGGGAAAAATGTGTTTGAATATCCCGAAGGCAAAAAGCTAGCAAGTGATGAAATTGTCTCT
GTTCCCTGCGACATCTGGATTCCGGCAGCCAGACCTGATGTTATAAACGGGAATAATGTCCATCTTCTGGATACGAAACT
GGTAGTTGAGGGAGCAAACATACCCCTCACTGGGGAGGCTGAAAAAATTCTCTATGATAAAGGAATTCTTTATGTTCCGG
ACTTTATTGCAAATGCAGGTGGGGTTATCTGTGCTGCCTCGGAGCACCAGAAAACTACCAGATCCATAGTTTTCGGTTTA
ATTGAAGAAAAGGTTAGAAGCAATACGGAACAGGTGCTGGAAGCTTCAAAAACCAGAGGAATACTGCCAAGGGAAGCTGC
TTTTGAACTTGCCATAAAAAGAGTTAACAGGGCAATGAAGTACAGGCGCTGGTCGATCTTTTCTTCTGCCCCGGGATTTG
TATAA

Upstream 100 bases:

>100_bases
AACTATAATAGGTCTCTGTAACATATCTTTCTTAGTCTGAGATTCATGCAACATTAGAACCTCGGAATACAGATCATAGG
GGGATAAGGTAACGGAGGAT

Downstream 100 bases:

>100_bases
CTTTAAGCTACGCTCCGGGATTTGTATAACTTTAAGCTATTTTCCAGTAGAGCCTTTAATTTTTCAGGCTTCCCGGAATT
CTTAGCTCTTTTATCTTTCA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH-2 [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MFRFADELGPFKIIHIYEPSIDLKAVLVVDNIARGPALGGVRIAPDVSAEECFRLARAMTLKNAAADLPYGGGKIVVYGD
PKMPFEKKSQLLRALAGALRYTEEYIFAPDMGTDEICMACIKDEIGRVVGLPCEMGGIPLDEVGATGWGLFNATEVALKY
CDFELKGARVVIQGFGAVGKHAARFLVRKGAVLVGVADSRGAVHNPEGLDVDSLIALKNEGKNVFEYPEGKKLASDEIVS
VPCDIWIPAARPDVINGNNVHLLDTKLVVEGANIPLTGEAEKILYDKGILYVPDFIANAGGVICAASEHQKTTRSIVFGL
IEEKVRSNTEQVLEASKTRGILPREAAFELAIKRVNRAMKYRRWSIFSSAPGFV

Sequences:

>Translated_374_residues
MFRFADELGPFKIIHIYEPSIDLKAVLVVDNIARGPALGGVRIAPDVSAEECFRLARAMTLKNAAADLPYGGGKIVVYGD
PKMPFEKKSQLLRALAGALRYTEEYIFAPDMGTDEICMACIKDEIGRVVGLPCEMGGIPLDEVGATGWGLFNATEVALKY
CDFELKGARVVIQGFGAVGKHAARFLVRKGAVLVGVADSRGAVHNPEGLDVDSLIALKNEGKNVFEYPEGKKLASDEIVS
VPCDIWIPAARPDVINGNNVHLLDTKLVVEGANIPLTGEAEKILYDKGILYVPDFIANAGGVICAASEHQKTTRSIVFGL
IEEKVRSNTEQVLEASKTRGILPREAAFELAIKRVNRAMKYRRWSIFSSAPGFV
>Mature_374_residues
MFRFADELGPFKIIHIYEPSIDLKAVLVVDNIARGPALGGVRIAPDVSAEECFRLARAMTLKNAAADLPYGGGKIVVYGD
PKMPFEKKSQLLRALAGALRYTEEYIFAPDMGTDEICMACIKDEIGRVVGLPCEMGGIPLDEVGATGWGLFNATEVALKY
CDFELKGARVVIQGFGAVGKHAARFLVRKGAVLVGVADSRGAVHNPEGLDVDSLIALKNEGKNVFEYPEGKKLASDEIVS
VPCDIWIPAARPDVINGNNVHLLDTKLVVEGANIPLTGEAEKILYDKGILYVPDFIANAGGVICAASEHQKTTRSIVFGL
IEEKVRSNTEQVLEASKTRGILPREAAFELAIKRVNRAMKYRRWSIFSSAPGFV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=331, Percent_Identity=32.9305135951662, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI4885281, Length=331, Percent_Identity=32.02416918429, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1788059, Length=327, Percent_Identity=29.0519877675841, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17544676, Length=333, Percent_Identity=31.2312312312312, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI45549226, Length=339, Percent_Identity=33.0383480825959, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45553475, Length=334, Percent_Identity=33.2335329341317, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24649460, Length=314, Percent_Identity=32.8025477707006, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24649283, Length=332, Percent_Identity=32.8313253012048, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 40534; Mature: 40534

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRFADELGPFKIIHIYEPSIDLKAVLVVDNIARGPALGGVRIAPDVSAEECFRLARAMT
CCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHH
LKNAAADLPYGGGKIVVYGDPKMPFEKKSQLLRALAGALRYTEEYIFAPDMGTDEICMAC
HHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
IKDEIGRVVGLPCEMGGIPLDEVGATGWGLFNATEVALKYCDFELKGARVVIQGFGAVGK
HHHHHHHEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCEEECCCEEEEEECCHHHH
HAARFLVRKGAVLVGVADSRGAVHNPEGLDVDSLIALKNEGKNVFEYPEGKKLASDEIVS
HHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHEEEECCCCCCEEECCCCCCCCCCCEEE
VPCDIWIPAARPDVINGNNVHLLDTKLVVEGANIPLTGEAEKILYDKGILYVPDFIANAG
CCCCEEECCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCHHHEEECCCCEECCHHHCCCC
GVICAASEHQKTTRSIVFGLIEEKVRSNTEQVLEASKTRGILPREAAFELAIKRVNRAMK
CEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YRRWSIFSSAPGFV
HHHHEEECCCCCCC
>Mature Secondary Structure
MFRFADELGPFKIIHIYEPSIDLKAVLVVDNIARGPALGGVRIAPDVSAEECFRLARAMT
CCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHH
LKNAAADLPYGGGKIVVYGDPKMPFEKKSQLLRALAGALRYTEEYIFAPDMGTDEICMAC
HHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
IKDEIGRVVGLPCEMGGIPLDEVGATGWGLFNATEVALKYCDFELKGARVVIQGFGAVGK
HHHHHHHEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCEEECCCEEEEEECCHHHH
HAARFLVRKGAVLVGVADSRGAVHNPEGLDVDSLIALKNEGKNVFEYPEGKKLASDEIVS
HHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHEEEECCCCCCEEECCCCCCCCCCCEEE
VPCDIWIPAARPDVINGNNVHLLDTKLVVEGANIPLTGEAEKILYDKGILYVPDFIANAG
CCCCEEECCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCHHHEEECCCCEECCHHHCCCC
GVICAASEHQKTTRSIVFGLIEEKVRSNTEQVLEASKTRGILPREAAFELAIKRVNRAMK
CEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
YRRWSIFSSAPGFV
HHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726; 1730244 [H]