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The map label for this gene is opuCB [H]

Identifier: 21226398

GI number: 21226398

Start: 396185

End: 396847

Strand: Reverse

Name: opuCB [H]

Synonym: MM_0296

Alternate gene names: 21226398

Gene position: 396847-396185 (Counterclockwise)

Preceding gene: 21226399

Following gene: 21226397

Centisome position: 9.69

GC content: 47.81

Gene sequence:

>663_bases
ATGATTATTGAAGGTCTTAACGATTTCACTGCAGAAATAATAAGAGTCTGGAACACTCAGATGCTCTCCCTCCGCACAGT
TGAACATCTCTACATGTTTTTCTTTGCCCTCTTTTTTTCTATCCTTATTGGTGTAGTCACAGGAGTTCTTACCTACAGGA
ACCAGAAACTTGCAGGCCCCGTCCTTAACAGCCTGAACGTGGTAGAAACAGTCCCTGATGTGGCTCTGCTTGTCCTTCTG
CTTCCCATATTCGGGATAGGTGCTGCACCGACAATAGTTGCTTCTATCCTCTATTCTATCCTCCCTATTGCCAGAGATAC
CTATACAGGCCTTTCAAACGTGCCCAGAGAATACATAGAAATTGCCGAAGCCCTGGGACTGACTCCCCGAGAAGTCCTTT
TCAAAGTCCGTTTTCCCCTTTCCCTGCCTCTGATTGCCGGAGGCGTCAGGATTGCGGTCGTATTTACAATGGGGGTTGTA
ACCCTTGGAGGTCTGATTGCTGCAGGCGGGCTTGGGGCTGCCCTTCAGAACGGAATTCAACTGTATGATATGGGCACAAT
CCTTGTTACGGGTGCCTGGGTGGGGCTTCTTGCAGTGCTTCTGGACGGAATAGCAGGTATAATAGAAAAAATTCTTAAAG
CGAGGTACGGGACATGGTCATAA

Upstream 100 bases:

>100_bases
GTGATAGCAAAAATAATTACCCGCATACTGAAGAGATGATCCAGTTTAAAATCAAACCACGTGCAGAGGTACAGGAGCAT
ATACCACTTGAGGTGATCGT

Downstream 100 bases:

>100_bases
CTGCAGAAATTCTTGAGGCAACGCTTGAACACCTTTTCCTTGCTTATACGGCTCTTCTTGTCGGAGCCCTTGTGTCCGTT
CCTCTGATATTCCTGTCCCT

Product: glycine betaine transport system, permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MIIEGLNDFTAEIIRVWNTQMLSLRTVEHLYMFFFALFFSILIGVVTGVLTYRNQKLAGPVLNSLNVVETVPDVALLVLL
LPIFGIGAAPTIVASILYSILPIARDTYTGLSNVPREYIEIAEALGLTPREVLFKVRFPLSLPLIAGGVRIAVVFTMGVV
TLGGLIAAGGLGAALQNGIQLYDMGTILVTGAWVGLLAVLLDGIAGIIEKILKARYGTWS

Sequences:

>Translated_220_residues
MIIEGLNDFTAEIIRVWNTQMLSLRTVEHLYMFFFALFFSILIGVVTGVLTYRNQKLAGPVLNSLNVVETVPDVALLVLL
LPIFGIGAAPTIVASILYSILPIARDTYTGLSNVPREYIEIAEALGLTPREVLFKVRFPLSLPLIAGGVRIAVVFTMGVV
TLGGLIAAGGLGAALQNGIQLYDMGTILVTGAWVGLLAVLLDGIAGIIEKILKARYGTWS
>Mature_220_residues
MIIEGLNDFTAEIIRVWNTQMLSLRTVEHLYMFFFALFFSILIGVVTGVLTYRNQKLAGPVLNSLNVVETVPDVALLVLL
LPIFGIGAAPTIVASILYSILPIARDTYTGLSNVPREYIEIAEALGLTPREVLFKVRFPLSLPLIAGGVRIAVVFTMGVV
TLGGLIAAGGLGAALQNGIQLYDMGTILVTGAWVGLLAVLLDGIAGIIEKILKARYGTWS

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1174

COG function: function code E; ABC-type proline/glycine betaine transport systems, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=177, Percent_Identity=33.8983050847458, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788451, Length=204, Percent_Identity=32.3529411764706, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1788449, Length=201, Percent_Identity=26.3681592039801, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 23616; Mature: 23616

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIEGLNDFTAEIIRVWNTQMLSLRTVEHLYMFFFALFFSILIGVVTGVLTYRNQKLAGP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VLNSLNVVETVPDVALLVLLLPIFGIGAAPTIVASILYSILPIARDTYTGLSNVPREYIE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHH
IAEALGLTPREVLFKVRFPLSLPLIAGGVRIAVVFTMGVVTLGGLIAAGGLGAALQNGIQ
HHHHHCCCHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCE
LYDMGTILVTGAWVGLLAVLLDGIAGIIEKILKARYGTWS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIIEGLNDFTAEIIRVWNTQMLSLRTVEHLYMFFFALFFSILIGVVTGVLTYRNQKLAGP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VLNSLNVVETVPDVALLVLLLPIFGIGAAPTIVASILYSILPIARDTYTGLSNVPREYIE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHH
IAEALGLTPREVLFKVRFPLSLPLIAGGVRIAVVFTMGVVTLGGLIAAGGLGAALQNGIQ
HHHHHCCCHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCE
LYDMGTILVTGAWVGLLAVLLDGIAGIIEKILKARYGTWS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10216873; 9384377 [H]