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The map label for this gene is rfaE [C]

Identifier: 21226344

GI number: 21226344

Start: 325004

End: 325459

Strand: Direct

Name: rfaE [C]

Synonym: MM_0242

Alternate gene names: 21226344

Gene position: 325004-325459 (Clockwise)

Preceding gene: 21226340

Following gene: 21226356

Centisome position: 7.93

GC content: 46.71

Gene sequence:

>456_bases
ATGCTTGCAGGATATTACCCGAGTCCAGGTGATTTATTGACGCGTGTATTGGCTACCGGAACTTTTGATATTCTCCATCC
GGGACATGTTTATTTCCTCGCCCAGGCGAAGGCTCTCGGAGACGAGCTCTTTGTCATTATTGCCAGGGATTCCAATGTGA
CCCATAAGCCGAAACCAGTGATTCCCGAGGAACAGCGGCTTGAGATGGTAGATGCACTCAAAGCGGTAAATAAAGCCATT
CTTGGCAGTGAAAAGGACATGTTCGAACCTCTCAGGGAGATCAAACCGGACATCATTGCTCTGGGCTATGACCAGCGTTT
TGACACAGAGATTCTGGAAAAAGAACTTACAAAAAGAGGGCTTCCTGCAAAAGTAGTAAGGATCCCGCTTTCAAAAGAAT
GCCCCCTTTGCAGTACCGGCACAATTATAAAAGAAGTCCTTAAACGGTACGGGTAA

Upstream 100 bases:

>100_bases
TTATTTTCGGAAAAATCAACGACTGTCCACATTATCTACAGGCTGTATAACATGGAATTCTTACCTGCAGGGAAATGAAG
TTATTTTCAGTAAGTTTCTT

Downstream 100 bases:

>100_bases
ACTTACCCATACCGCCTGCTGTTTTATGCAAACACTTTTTCAAGCCTGTCAAGGGCTTCACGAATTCTATCAATGGACGT
TGCATAGGAAATCCGGATAA

Product: glycerol-3-phosphate cytidylyltransferase

Products: diphosphate; CDPglycerol

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MLAGYYPSPGDLLTRVLATGTFDILHPGHVYFLAQAKALGDELFVIIARDSNVTHKPKPVIPEEQRLEMVDALKAVNKAI
LGSEKDMFEPLREIKPDIIALGYDQRFDTEILEKELTKRGLPAKVVRIPLSKECPLCSTGTIIKEVLKRYG

Sequences:

>Translated_151_residues
MLAGYYPSPGDLLTRVLATGTFDILHPGHVYFLAQAKALGDELFVIIARDSNVTHKPKPVIPEEQRLEMVDALKAVNKAI
LGSEKDMFEPLREIKPDIIALGYDQRFDTEILEKELTKRGLPAKVVRIPLSKECPLCSTGTIIKEVLKRYG
>Mature_151_residues
MLAGYYPSPGDLLTRVLATGTFDILHPGHVYFLAQAKALGDELFVIIARDSNVTHKPKPVIPEEQRLEMVDALKAVNKAI
LGSEKDMFEPLREIKPDIIALGYDQRFDTEILEKELTKRGLPAKVVRIPLSKECPLCSTGTIIKEVLKRYG

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0615

COG function: function code MI; Cytidylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789432, Length=134, Percent_Identity=33.5820895522388, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2 [H]

EC number: 2.7.7.39

Molecular weight: Translated: 16859; Mature: 16859

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAGYYPSPGDLLTRVLATGTFDILHPGHVYFLAQAKALGDELFVIIARDSNVTHKPKPV
CCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCEEEEEEECCCCCCCCCCCC
IPEEQRLEMVDALKAVNKAILGSEKDMFEPLREIKPDIIALGYDQRFDTEILEKELTKRG
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCC
LPAKVVRIPLSKECPLCSTGTIIKEVLKRYG
CCEEEEECCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLAGYYPSPGDLLTRVLATGTFDILHPGHVYFLAQAKALGDELFVIIARDSNVTHKPKPV
CCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCEEEEEEECCCCCCCCCCCC
IPEEQRLEMVDALKAVNKAILGSEKDMFEPLREIKPDIIALGYDQRFDTEILEKELTKRG
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCC
LPAKVVRIPLSKECPLCSTGTIIKEVLKRYG
CCEEEEECCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; sn-glycerol 3-phosphate

Specific reaction: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]